1. In Situ Real-Time Monitoring for Aseptic Drilling: Lessons Learned from the Atacama Rover Astrobiology Drilling Studies Contamination Control Strategy and Implementation and Application to the Icebreaker Mars Life Detection Mission
- Author
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National Aeronautics and Space Administration (US), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Bonaccorsi, Rosalba, Glass, Brian J., Moreno-Paz, Mercedes, García-Villadangos, Miriam, Warren-Rhodes, Kimberley, Parro-García, Víctor, Wilhite, Patrick, McKay, Christopher P., National Aeronautics and Space Administration (US), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Bonaccorsi, Rosalba, Glass, Brian J., Moreno-Paz, Mercedes, García-Villadangos, Miriam, Warren-Rhodes, Kimberley, Parro-García, Víctor, Wilhite, Patrick, and McKay, Christopher P.
- Abstract
In 2019, the Atacama Rover Astrobiology Drilling Studies (ARADS) project field-tested an autonomous rover-mounted robotic drill prototype for a 6-Sol life detection mission to Mars (Icebreaker). ARADS drilled Mars-like materials in the Atacama Desert (Chile), one of the most life-diminished regions on Earth, where mitigating contamination transfer into life-detection instruments becomes critical. Our Contamination Control Strategy and Implementation (CCSI) for the Sample Handling and Transfer System (SHTS) hardware (drill, scoop and funnels) included out-of-simulation protocol testing (out-of-sim) for hardware decontamination and verification during the 6-Sol simulation (in-sim). The most effective five-step decontamination combined safer-to-use sterilants (3%_hydrogen-peroxide-activated 5%_sodium-hypochlorite), and in situ real-time verification by adenosine triphosphate (ATP) and Signs of Life Detector (SOLID) Fluorescence Immunoassay characterization of hardware bioburden and airborne contaminants. The 20- to 40-min protocol enabled a 4-log bioburden reduction down to <0.1 fmoles ATP detection limit (funnels and drill) to 0.2–0.7 fmoles (scoop) of total ATP. The (post-cleaning) hardware background was 0.3 to 1–2 attomoles ATP/cm2 (cleanliness benchmark background values) equivalent to ca. 1–10 colony forming unit (CFU)/cm2. Further, 60–100% of the in-sim hardware background was ≤3–4 bacterial cells/cm2, the threshold limit for Class <7 aseptic operations. Across the six Sols, the flux of airborne contaminants to the drill sites was ∼5 and ∼22 amoles ATP/(cm2·day), accounting for an unexpectedly high Fluorescence Intensity (FI) signal (FI: ∼6000) against aquatic cyanobacteria, but negligible anthropogenic contribution. The SOLID immunoassay also detected microorganisms from multiple habitats across the Atacama Desert (anoxic, alkaline/acidic microenvironments in halite fields, playas, and alluvial fans) in both airborne and post-cleaning hardware background. Final
- Published
- 2023