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Your search keyword '"Wieghofer, Peter"' showing total 18 results
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18 results on '"Wieghofer, Peter"'

2. Myelin insulation as a risk factor for axonal degeneration in autoimmune demyelinating disease

Author

Schäffner, Erik, Bosch-Queralt, Mar, Edgar, Julia M., Lehning, Maria, Strauß, Judith, Fleischer, Niko, Kungl, Theresa, Wieghofer, Peter, Berghoff, Stefan A., Reinert, Tilo, Krueger, Martin, Morawski, Markus, Möbius, Wiebke, Barrantes-Freer, Alonso, Stieler, Jens, Sun, Ting, Saher, Gesine, Schwab, Markus H., Wrede, Christoph, Frosch, Maximilian, Prinz, Marco, Reich, Daniel S., Flügel, Alexander, Stadelmann, Christine, Fledrich, Robert, Nave, Klaus-Armin, and Stassart, Ruth M.

Published
2023
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4. Redefining the ontogeny of hyalocytes as yolk sac-derived tissue-resident macrophages of the vitreous body.

Author

Rosmus, Dennis-Dominik, Koch, Jana, Hausmann, Annika, Chiot, Aude, Arnhold, Franz, Masuda, Takahiro, Kierdorf, Katrin, Hansen, Stefanie Marie, Kuhrt, Heidrun, Fröba, Janine, Wolf, Julian, Boneva, Stefaniya, Gericke, Martin, Ajami, Bahareh, Prinz, Marco, Lange, Clemens, and Wieghofer, Peter

Subjects
VITREOUS body, MACROPHAGE colony-stimulating factor, SENSE organs, MYELOID cells, MACROPHAGES
Abstract

Background: The eye is a highly specialized sensory organ which encompasses the retina as a part of the central nervous system, but also non-neural compartments such as the transparent vitreous body ensuring stability of the eye globe and a clear optical axis. Hyalocytes are the tissue-resident macrophages of the vitreous body and are considered to play pivotal roles in health and diseases of the vitreoretinal interface, such as proliferative vitreoretinopathy or diabetic retinopathy. However, in contrast to other ocular macrophages, their embryonic origin as well as the extent to which these myeloid cells might be replenished by circulating monocytes remains elusive. Results: In this study, we combine transgenic reporter mice, embryonic and adult fate mapping approaches as well as parabiosis experiments with multicolor immunofluorescence labeling and confocal laser-scanning microscopy to comprehensively characterize the murine hyalocyte population throughout development and in adulthood. We found that murine hyalocytes express numerous well-known myeloid cell markers, but concomitantly display a distinct immunophenotype that sets them apart from retinal microglia. Embryonic pulse labeling revealed a yolk sac-derived origin of murine hyalocytes, whose precursors seed the developing eye prenatally. Finally, postnatal labeling and parabiosis established the longevity of hyalocytes which rely on Colony Stimulating Factor 1 Receptor (CSF1R) signaling for their maintenance, independent of blood-derived monocytes. Conclusion: Our study identifies hyalocytes as long-living progeny of the yolk sac hematopoiesis and highlights their role as integral members of the innate immune system of the eye. As a consequence of their longevity, immunosenescence processes may culminate in hyalocyte dysfunction, thereby contributing to the development of vitreoretinal diseases. Therefore, myeloid cell-targeted therapies that convey their effects through the modification of hyalocyte properties may represent an interesting approach to alleviate the burden imposed by diseases of the vitreoretinal interface. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Transcriptional and Distributional Profiling of Microglia in Retinal Angiomatous Proliferation

Author

Schlecht, Anja, Wolf, Julian, Boneva, Stefaniya, Prinz, Gabriele, Braunger, Barbara M., Wieghofer, Peter, Agostini, Hansjürgen, Schlunck, Günther, Lange, Clemens, Schlecht, Anja, Wolf, Julian, Boneva, Stefaniya, Prinz, Gabriele, Braunger, Barbara M., Wieghofer, Peter, Agostini, Hansjürgen, Schlunck, Günther, and Lange, Clemens

Abstract

Macular neovascularization type 3, formerly known as retinal angiomatous proliferation (RAP), is a hallmark of age-related macular degeneration and is associated with an accumulation of myeloid cells, such as microglia (MG) and infiltrating blood-derived macrophages (MAC). However, the contribution of MG and MAC to the myeloid cell pool at RAP sites and their exact functions remain unknown. In this study, we combined a microglia-specific reporter mouse line with a mouse model for RAP to identify the contribution of MG and MAC to myeloid cell accumulation at RAP and determined the transcriptional profile of MG using RNA sequencing. We found that MG are the most abundant myeloid cell population around RAP, whereas MAC are rarely, if ever, associated with late stages of RAP. RNA sequencing of RAP-associated MG showed that differentially expressed genes mainly contribute to immune-associated processes, including chemotaxis and migration in early RAP and proliferative capacity in late RAP, which was confirmed by immunohistochemistry. Interestingly, MG upregulated only a few angiomodulatory factors, suggesting a rather low angiogenic potential. In summary, we showed that MG are the dominant myeloid cell population at RAP sites. Moreover, MG significantly altered their transcriptional profile during RAP formation, activating immune-associated processes and exhibiting enhanced proliferation, however, without showing substantial upregulation of angiomodulatory factors.

Published
2022

10. Comparative transcriptome analysis of human and murine choroidal neovascularization identifies fibroblast growth factor inducible-14 as phylogenetically conserved mediator of neovascular age-related macular degeneration

Author

Wolf, Julian, primary, Schlecht, Anja, additional, Rosmus, Dennis-Dominik, additional, Boneva, Stefaniya, additional, Agostini, Hansjürgen, additional, Schlunck, Günther, additional, Wieghofer, Peter, additional, and Lange, Clemens, additional

Published
2022
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14. Hyalocyte origin, structure, and imaging

Author

Wieghofer, Peter, Engelbert, Michael, Chui, Toco Y. P., Rosen, Richard B., Sakamoto, Taiji, and Sebag, J.

Subjects
Ophthalmology, Biomedical Engineering, ddc:610, Article, Optometry
Abstract

INTRODUCTION: Hyalocytes have been recognized as resident tissue macrophages of the vitreous body since the mid-19(th) century. Despite this, knowledge about their origin, turnover, and dynamics is limited. AREAS COVERED: Historically, initial studies on the origin of hyalocytes used light and electron microscopy. Modern investigations across species including rodents and humans will be described. Novel imaging is now available to study human hyalocytes in vivo. The shared ontogeny with retinal microglia and their eventual interdependence as well as differences will be discussed. EXPERT OPINION: Owing to a common origin as myeloid cells, hyalocytes and retinal microglia have similarities, but hyalocytes appear to be distinct as resident macrophages of the vitreous body.

Published
2022

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