Search

ObjectiveCarotid artery stenosis, primarily caused by atherosclerosis, is a major risk factor for ischemic stroke. Carotid endarterectomy (CEA) and carotid artery stenting (CAS) are established interventions to reduce stroke risk and restore cerebral blood flow. However, the effect of these treatments on circadian rhythms, and their influence on stroke recovery, remains underexplored. This study aims to assess how disruptions in circadian rhythms—specifically sleep quality and blood pressure variability—impact recovery in patients undergoing CEA or CAS.MethodsWe conducted a prospective study involving 177 patients with carotid artery stenosis, all treated with either CEA or CAS. Patients were followed for 90 days post-treatment, with neurological outcomes evaluated using the NIHSS Stroke Scale (NIHSS). Circadian rhythm-related factors, including sleep quality (Pittsburgh Sleep Quality Index [PSQI]) and blood pressure variability (daytime systolic and nighttime diastolic BP), were assessed pre-and post-treatment. Stepwise regression was used to identify predictors of stroke recovery.ResultsIn a cohort of 177 patients with symptomatic carotid atherosclerotic stenosis, stepwise regression identified post-treatment changes in PSQI, nighttime diastolic blood pressure, and the presence of coronary heart disease as significant independent predictors of poor neurological outcomes (p

With the progress of communication technology, the cost of optical fiber and 5G continues to decrease, and data transmission becomes more convenient and fast, making it possible to realize differential protection of distribution network by various intelligent algorithms using signal waveforms. Aiming at the problem that the traditional relay protection device can not meet the actual demand when the single-phase ground fault occurs in the distribution network with distributed generation, this paper proposes a new differential protection scheme. The characteristic mode decomposition improved by the whale optimization algorithm is used to decompose the zero-sequence current waveform collected at both ends of the line. Based on the basic principle of current differential protection, the derivative dynamic time warping of the component with the largest fault feature can effectively solve the problem that the grounding current of the distribution network cannot meet the working requirements of the differential protection device, and ensure the safe and stable operation of the system. Finally, based on MATLAB software, the performance of this method is comprehensively evaluated by simulating different fault conditions, so as to ensure the feasibility and accuracy of this method in the case of diversified faults when the distributed generation is used as part of the power supply.

Abstract Oncolytic viruses (OVs) represent a new class of multi-modal immunotherapies for cancer, with OV-elicited antitumor immunity being key to their overall therapeutic efficacy. Currently, the clinical effectiveness of OV as monotherapy remains limited, and thus investigators have been exploring various combinations with other anti-cancer agents and demonstrated improved therapeutic efficacy. As cancer cells have evolved to alter key signaling pathways for enhanced cell proliferation, cancer progression and metastasis, these cellular and molecular changes offer promising targets for rational cancer therapy design. In this regard, key molecules in relevant signaling pathways for cancer cells or/and immune cells, such as EGFR-KRAS (e.g., KRASG12C), PI3K-AKT-mTOR, ERK-MEK, JAK-STAT, p53, PD-1-PD-L1, and epigenetic, or immune pathways (e.g., histone deacetylases, cGAS-STING) are currently under investigation and have the potential to synergize with OV to modulate the immune milieu of the tumor microenvironment (TME), thereby improving and sustaining antitumor immunity. As many small molecule modulators of these signaling pathways have been developed and have shown strong therapeutic potential, here we review key findings related to both OV-mediated immunotherapy and the utility of small molecule modulators of signaling pathways in immuno-oncology. Then, we focus on discussion of the rationales and potential strategies for combining OV with selected modulators targeting key cellular signaling pathways in cancer or/and immune cells to modulate the TME and enhance antitumor immunity and therapeutic efficacy. Finally, we provide perspectives and viewpoints on the application of novel experimental systems and technologies that can propel this exciting branch of medicine into a bright future.

In response to the deformation resistance deficiency and poor toughness characteristics of soil after microbial curing, a combination of fibre reinforcement technology and microbial curing technology was used to conduct microbial curing tests using basalt fibres and denitrifying bacteria. In this paper, the effects of fibre on the strength and toughness of soil consolidation were analysed by unconfined compressive strength test and direct shear test, and the stability of reinforced slope was analysed by numerical simulation. The results show the following. (1) Basalt fibre can effectively improve the characteristics of brittle damage of microbially consolidated soil while increasing the compressive and shear strength. (2) Fibre dosing and fibre length have important effects on the mechanical properties of microbially consolidated soil. (3) The appropriate amount of basalt fibre can promote the generation of calcium carbonate. (4) The plastic strain area of the slope decreases after microbial reinforcement and the maximum equivalent plastic stress decreases by 65 kPa.

The COVID-19 outbreak has been a public health crisis of international concern, causing huge impact on people’s lives. As an important part of social public crisis management, how to quickly and effectively raise resources to participate in emergency relief in the era of self-media is a common challenge faced by global charitable organizations. This article attempts to use empirical evidence from Tencent charitable crowdfunding platform, the largest charitable crowdfunding platform in China, to answer this question. We consider 205 COVID-19 charitable projects and 11,177,249 donors to assess the process by which non-profit organizations raise funds through the information about project descriptions. Based on the effects of information and emotional framing, we explore the effects of the readability (i.e., complexity and understandability) and negative tone of the project description on fundraising amount. We then investigate the mediating role of forwarding times, as affective response to the text might explain forwarding times, which in turn affects money raised by increasing the visibility of the campaign. On this basis, the moderating role of recipient’s crisis involvement is tested during this process. The empirical results indicate that the complexity of the description will reduce the fundraising amount, while understandability and negative tone help to improve it. Furthermore, we found that forwarding times played an important mediating role in this process. Then the buffer effect of crisis involvement on the negative effect of complexity was validated, and its amplification on the positive effects of understandability was also verified.

Dexmedetomidine displays multiple mechanisms of neuroprotection in ameliorating ischemic brain injury. In this study, we explored the beneficial effects of dexmedetomidine on blood-brain barrier (BBB) integrity and neuroinflammation in cerebral ischemia/reperfusion injury. Sprague-Dawley rats were subjected to middle cerebral artery occlusion (MCAO) for 1.5 h and reperfusion for 24 h to establish a rat model of cerebral ischemia/reperfusion injury. Dexmedetomidine (9 g/kg) was administered to rats 30 min after MCAO through intravenous injection, and SB203580 (a p38 MAPK inhibitor, 200 g/kg) was injected intraperitoneally 30 min before MCAO. Brain damages were evaluated by 2,3,5-triphenyltetrazolium chloride staining, hematoxylin-eosin staining, Nissl staining, and brain water content assessment. BBB permeability was examined by Evans blue staining. Expression levels of claudin-5, zonula occludens-1, occludin, and matrix metalloproteinase-9 (MMP-9) as well as M1/M2 phenotypes-associated markers were assessed using immunofluorescence, RT-qPCR, Western blotting, and gelatin zymography. Enzyme-linked immunosorbent assay was used to examine inflammatory cytokine levels. We found that dexmedetomidine or SB203580 attenuated infarct volume, brain edema, BBB permeability, and neuroinflammation, and promoted M2 microglial polarization after cerebral ischemia/reperfusion injury. Increased MMP-9 activity by ischemia/reperfusion injury was inhibited by dexmedetomidine or SB203580. Dexmedetomidine inhibited the activation of the ERK, JNK, and p38 MAPK pathways. Moreover, activation of JNK or p38 MAPK reversed the protective effects of dexmedetomidine against ischemic brain injury. Overall, dexmedetomidine ameliorated brain injury by alleviating BBB permeability and promoting M2 polarization in experimental cerebral ischemia/reperfusion injury model by inhibiting the activation of JNK and p38 MAPK pathways. [ABSTRACT FROM AUTHOR]

Under the background of the shortage of traditional energy and the increasingly serious environmental problems, this paper proposes a bi-level optimization configuration method for microgrids based on stepped carbon trading and price-based demand response. The stepped carbon trading mechanism is introduced into the planning model to clarify the impact of construction processes on land carbon emissions. Simultaneously, price-based demand response during system operation is introduced, guiding users to change the electricity consumption strategies to promote the consumption of renewable energy. Then, a bi-level optimal configuration framework is constructed. The upper level is optimized to allocate the capacity of the microgrid, with the lowest annual equivalent comprehensive cost as the objective function. The lower level is optimized for microgrid operation, with the minimum sum of the microgrid operation cost and carbon emission cost as the objective function. In view of the advantages of enhanced whale optimization algorithm (E-WOA) in solving multidimensional feature selection problems, E-WOA and CPLEX solver are combined to solve the bi-level optimization model for the first time. Simulation results show that after introducing a stepped carbon trading mechanism, the system carbon emissions decreased by 23.09%. Considering price-based demand response under the premise of introducing a stepped carbon trading mechanism, the total cost decreased by 3.57% and the carbon emissions decreased by 19.83%. [ABSTRACT FROM AUTHOR]

Objective To evaluate the clinical efficacy of Mulligan mobilization with movement （MWM） technique combined with dynamic interference electrotherapy in the treatment of cervical spondylosis. Methods A total of 42 patients with cervical spondylosis were retrospectively selected and divided into the observation group （MWM combined with dynamic interference electrotherapy, n = 21） and control group （dynamic interference electrotherapy only, n = 21）. The visual analogue scale （VAS）, neck disability index （NDI）, Hamilton anxiety scale （HAMA） and Pittsburgh sleep quality index （PSQI） scores before treatment, at the end of treatment, at 1 and 3 months after treatment were all compared between two groups. Results In two groups, all parameters at different time points after corresponding treatment were significantly improved compared with those before treatment （all P < 0.05）. The VAS score at the end of treatment in the observation group was significantly lower than that in the control group （P < 0.05）. In the observation group, the HAMA scores at the end of treatment, at 1 and 3 months after treatment were significantly lower, and the PSQI score was significantly lower compared with those in the control group （all P < 0.05）. Conclusion In addition to relieving neck pain and improving cervical spine function, MWM combined with dynamic interference electrotherapy can also alleviate anxiety and enhance sleep quality in patients with cervical spondylosis.