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Abstract The gut microbiota offers an extensive resource of enzymes, but many remain uncharacterized. To distinguish the activities of similar annotated proteins and mine the potentially applicable ones in the microbiome, we applied an effective Activity-Based Metaproteomics (ABMP) strategy using a specific activity-based probe (ABP) to screen the entire gut microbiome for directly discovering active enzymes and their potential applications, not for exploring host-microbiome interactions. By using an activity-based cyclophellitol aziridine probe specific to α-galactosidases (AGAL), we successfully identified and characterized several gut microbiota enzymes possessing AGAL activities. Cryo-electron microscopy analysis of a newly characterized enzyme (AGLA5) revealed the covalent binding conformations between the AGAL5 active site and the cyclophellitol aziridine ABP, which could provide insights into the enzyme’s catalytic mechanism. The four newly characterized AGALs have diverse potential activities, including raffinose family oligosaccharides (RFOs) hydrolysis and enzymatic blood group transformation. Collectively, we present a ABMP platform that facilitates gut microbiota AGALs discovery, biochemical activity annotations and potential industrial or biopharmaceutical applications.

Summary: An extended lithium-ion battery model is proposed, which simplifies the solid-liquid diffusion process compared with the full-order pseudo two-dimensional (P2D) model, in order to reduce computational complexity and enhance modeling speed. To simplify the model, the three-parameter method is utilized to simplify the solid-phase diffusion process. Meanwhile, exponential fitting is applied to simplify the liquid-phase diffusion process. In addition, the average current flux is used to simplify the current exchange density at the solid-liquid interface. Finally, experiments are performed to verify the model, and experimental results demonstrate that, even though fewer parameters need to be identified, the simplified model still has satisfied accuracy under constant current and dynamic situations.

Highlights The SiO2 nanofiber membranes and MXene@c-MWCNT6:4 as one unit layer (SMC1) were bonded together with 5 wt% PVA solution. When the structural unit is increased to three layers, the resulting SMC3 has an average electromagnetic interference SET of 55.4 dB and a low thermal conductivity of 0.062 W m−1 K−1. SMCx exhibit stable electromagnetic interference shielding and excellent thermal insulation even in extreme heat and cold environment.

Abstract Background Ubiquitin-conjugating enzyme E2 N (UBE2N) is recognized in the progression of some cancers; however, little research has been conducted to describe its role in prostate cancer. The purpose of this paper is to explore the function and mechanism of UBE2N in prostate cancer cells. Methods UBE2N expression was detected in Cancer Genome Atlas Prostate Adenocarcinoma (TCGA-PRAD) data, prostate cancer tissue microarrays, and prostate cancer cell lines, respectively. UBE2N knockdown or overexpression was used to analyze its role in cell viability and glycolysis of prostate cancer cells and tumor growth. XAV939 or Axin1 overexpression was co-treated with UBE2N overexpression to detect the involvement of the Wnt/β-catenin signaling and Axin1 in the UBE2N function. UBE2N interacting with Axin1 was analyzed by co-immunoprecipitation assay. Results UBE2N was upregulated in prostate cancer and the UBE2N-high expression correlated with the poor prognosis of prostate cancer. UBE2N knockdown inhibited cell viability and glycolysis in prostate cancer cells and restricted tumor formation in tumor-bearing mice. Wnt/β-catenin inhibition and Axin1 overexpression reversed the promoting viability and glycolysis function of UBE2N. UBE2N promoted Axin1 ubiquitination and decreased Axin1 protein level.

To solve the low power transfer efficiency and magnetic field leakage problems of cardiac pacemaker wireless powering, we proposed a wireless power supply system suitable for implanted cardiac pacemaker based on mu-negative (MNG) and mu-near-zero (MNZ) metamaterials. First, a hybrid metamaterial consisted of central MNG unit for magnetic field concentration and surrounding MNZ units for magnetic leakage shielding was established by theoretical calculation. Afterwards, the magnetic field distribution of wireless power supply system with MNG-MNZ metamaterial slab was acquired via finite element simulation and verified to be better than the distribution with conventional MNG slab deployed. Finally, an experimental platform of wireless power supply system was established with which power transfer experiment and system temperature rise experiment were conducted. Simulation and experimental results showed that the power transfer efficiency was improved from 44.44%, 19.42%, 8.63% and 6.19% to 55.77%, 62.39%, 20.81% and 14.52% at 9.6 mm, 20 mm, 30 mm and 50 mm, respectively. The maximum SAR acquired by SAR simulation under human body environment was -7.14 dbm and maximum reduction of the magnetic field strength around the receiving coil was 2.82 A/m. The maximum temperature rise during 30min charging test was 3.85°C, and the safety requirements of human bodies were met.

Abstract Background Oral squamous cell carcinoma in minors is considered to be a distinct entity from OSCC in older patients, with an uncertain etiology. Human papillomavirus (HPV) infection may trigger the initiation and promote the progression of OSCC, but these roles have not been firmly established.We aimed to explore the correlation between HPV infection and the development of oral squamous cell carcinoma in minors and know the characteristics of OSCC in young patients more thoroughly. Method From January 2013 to December 2022,6 cases of OSCC aged

IntroductionIn the fragrance and perfume industry, the controlled release of fragrances are crucial factors that contribute to consumer appeal and product quality enhancement. In this study, various aromatic active substances were extracted from dandelion root (DR), which was subsequently calcined to produce high-performance porous biochar material.MethodsThe dandelion root biochar (DRB) material was identified as promising adsorbents for the controlled release of fragrances. Furfuryl alcohol was chosen as the model fragrance for inclusion and controlled release studies.Results and discussionThe DRB exhibited a substantial specific surface area of 991.89 m2/g, facilitating efficient storage and controlled release capabilities. Additionally, the DRB’s high stability and porous nature facilitated rapid collection and efficient recyclability. This research significantly contributes to the development of a sustainable, zero-waste multistage utilization strategy for dandelion roots, indicating a potential applications in the food flavoring industry and environmental conservations.

Abstract Background This study aimed to analyze the effect of surgical compliance on the survival outcome of patients with meningioma and explore the factors affecting surgical compliance. Methods We selected data from the Surveillance, Epidemiology, and End Results database for 122,632 meningioma patients diagnosed between 2004 and 2018. The effect of surgical compliance on patients’ overall survival (OS) was analyzed through Cox regression and Kaplan–Meier curves. Independent risk factors for surgical compliance were identified through multifactorial logistic regression analyses to construct diagnostic nomograms, further assessed by receiver operating characteristic curves. Furthermore, we used univariate and multivariate logistic regression analyses to evaluate relevant variables linked to adherence with meningioma surgery. Moreover, 1:1 propensity score matching was applied to assess the validity of the results in patients with favorable and poor surgical compliance. Results A total of 48,735 were eligible from the initial cohort of 122,632 patients with meningioma. Among them, 45,038 (92.40%) exhibited good surgical compliance, while 3697 (7.60%) had poor surgical compliance. The rate of patients with good surgical compliance was significantly higher than that of patients with inadequate surgical compliance (p

IntroductionThe buffalo is an important domestic animal globally, providing milk, meat, and labor to more than 2 billion people in 67 countries. The rumen microorganisms of buffaloes play an indispensable role in enabling the healthy functionality and digestive function of buffalo organisms. Currently, there is a lack of clarity regarding the differences in the composition and function of rumen microorganisms among buffaloes at different growth stages.MethodsIn this study, metagenomics sequencing technology was applied to examine the compositional and functional differences of rumen microorganisms in adult and breastfed buffaloes.ResultsThe results revealed that the rumen of adult buffaloes had significantly higher levels of the following dominant genera: Prevotella, UBA1711, RF16, Saccharofermentans, F23-D06, UBA1777, RUG472, and Methanobrevibacter_A. Interestingly, the dominant genera specific to the rumen of adult buffaloes showed a significant positive correlation (correlation>0.5, p-value0.5, p-value

Abstract Background The association between triglyceride–glucose (TyG) index and poor prognosis remains controversial. Whether renal function status affects the ability of the TyG index to predict poor prognosis has not yet been elucidated and merits further studies. Methods This retrospective cohort study included 22,031 participants from communities in the U.S. By juxtaposing the TyG categories with the estimated glomerular filtration rate (eGFR, either

Abstract. Background:. Atherosclerosis-related diseases represent significant health issues among adults globally. Despite their widespread impact, comprehensive data concerning the global and national burden and trends of these diseases remain sparse. Our objective is to examine the trends in the burden of atherosclerosis among adults from 1990 to 2019 at both global and national levels. Methods:. We reported the average annual percentage changes (AAPCs) in prevalence, incidence, mortality, and disability-adjusted life years (DALYs) of atherosclerosis-related diseases (ischemic heart disease [IHD], ischemic stroke, and peripheral arterial disease [PAD]) at the global and national levels among individuals based on a trend analysis of the Global Burden of Diseases Study (GBD) 2019. We further analyzed these global trends as a function of age, gender, and the social development index. We also used joinpoint regression analysis to identify the year with the most substantial changes in global trends. Results:. Globally, the AAPC of IHD incidence rose from 1990 to 2019 (0.20; 95% confidence interval [CI], 0.12-0.28), with substantial surges in 1995, 2001, 2005, 2010, and 2017. Conversely, AAPC of IHD mortality rates exhibited a different trend until a rise in 2014. The AAPC of incidence rates of ischemic stroke and PAD also escalated during the same period, with respective 0.43 (95% CI, 0.39-0.48) and 0.13 (95% CI, 0.06-0.21). For ischemic stroke, both incidence and mortality soared in 2014, while PAD incidence declined in 1994 and 1998, then sharply climbed in 2016. Nationally, the Northern Mariana Islands experienced the steepest increase in IHD and PAD incidence and mortality between 1990 and 2019. China saw a significant rise in ischemic stroke incidence, whereas the highest mortality rate increase occurred in Timor-Leste. By sociodemographic index (SDI) quintile, low-middle-, middle-, and high-middle-SDI countries all showed upward trends in IHD, ischemic stroke, and PAD incidence. Simultaneously, IHD and ischemic stroke mortality rates, as well as DALYs, dropped in the low-, high-middle-, and high-SDI nations. However, PAD mortality rates and DALYs saw an uptick across all SDI quintiles. Regarding age demographics, a global decrease in the AAPC IHD incidence as noted in individuals above 55 years old, in contrast to an increase in the 20-55 age group during this period. AAPC of mortality rates for IHD, ischemic stroke, and PAD decreased across all ages. The AAPC showed an increase in IHD incidence in both genders. Conversely, IHD's DALYs saw a reduction in both males and females. Ischemic stroke patterns mirrored these trends, whereas all measures for PAD exhibited growth for both sexes. Conclusions:. From 1990 to 2019, there was an overall increasing trend in the global incidence of all three clinical manifestations of atherosclerosis. Between 1990 and 2019, both the mortality rate and DALYs for IHD and ischemic stroke declined across all age groups. Overall, the burden of atherosclerosis-related diseases has not significantly decreased and even shows signs of trending upward. These findings strongly suggest that despite some progress made, efforts to control atherosclerosis diseases globally need to be intensified.

Abstract Layered transition-metal oxide materials are ideal cathode candidates for sodium-ion batteries due to high specific energy, yet suffer severe interfacial instability and capacity fading owing to strongly nucleophilic surface. In this work, the interfacial stability of layered NaNi1/3Fe1/3Mn1/3O2 cathode was effectively enhanced by electrolyte optimization. And the interfacial chemistry between the cathode and four widely used electrolytes (EC/DMC, EC/EMC, EC/DEC and EC/PC) was elucidated through experiments and theoretical calculations. The Na+ solvation structures at cathode-electrolyte interface in all four electrolytes exhibited enhanced coordination due to high electron density and strong nucleophilicity of oxide surface, which promoted the electrolytes’ decomposition with decreased oxidation stability. Among them, the EC/DMC electrolyte showed the tightest solvation structure due to smaller molecular chains and stable electrochemistry, which derived an even and robust cathode electrolyte interphase. It effectively protected the cathode and facilitated the reversible Na+ transport during long cycles, enabling the batteries with a high capacity retention of 83.3% after 300 cycles. This work provides new insights into the role of electrode surface characteristics in interface chemistry that can guide the design of advanced electrode and electrolyte materials for rechargeable batteries.

Abstract Background Measurement of muscle mass is important in the diagnosis of sarcopenia. Current measurement equipment are neither cost‐effective nor standardized and cannot be used in a variety of medical settings. Some simple measurement tools have been proposed that are subjective and unvalidated. We aimed to develop and validate a new estimation equation in a more objective and standardized way, based on current proven variables that accurately reflect muscle mass. Methods Cross‐sectional analysis with The National Health and Nutrition Examination Survey database for equation development and validation. Overall, 9875 participants were included for development (6913 participants) and validation (2962 participants), for whom the database included demographic data, physical measurements, and main biochemical indicators. Appendicular skeletal muscle mass (ASM) was estimated by dual‐energy x‐ray absorptiometry (DXA) and low muscle mass was defined by reference to five international diagnostic criteria. Linear regression was used to estimate the logarithm of the actual ASM from demographic data, physical measurements, and biochemical indicators. Results This study of 9875 participants comprised 4492 females (49.0%), with a weighted mean (SE) age of 41.83 (0.36) years and range of 12 to 85 years. The estimated ASM equations performed well in the validation data set. The variability in estimated ASM was low compared with the actual ASM (R2: Equation 1 = 0.91, Equation 4 = 0.89), with low bias (median difference: Equation 1 = −0.64, Equation 4 = 0.07; root mean square error: Equation 1 = 1.70 [1.69–1.70], Equation 4 = 1.85 [1.84–1.86]), high precision (interquartile range of the differences: Equation 1 = 1.87, Equation 4 = 2.17), and high efficacy in diagnosing low muscle mass (area under the curve: Equation 1 = 0.91 to 0.95, Equation 4 = 0.90 to 0.94). Conclusions The estimated ASM equations are accurate and simple and can be routinely applied clinically to estimate ASM and thus assess sarcopenia.

Graphene was first employed to improve the mechanical properties of Nb/Nb5Si3 composite, which is a critical candidate for the next-generation of high-temperature structural materials for aero-engines. Microstructures and mechanical properties of graphene reinforced Nb/Nb5Si3 composites with interfacial nano-Nb4C3 phase were systematically investigated. The graphene reinforced Nb/Nb5Si3 composite with outstanding room temperature fracture toughness and compressive strength was fabricated successfully via spark plasma sintering. The microstructure of this composite changes from laminated morphology to uniformly distributed equiaxed microstructure, with the increase of fabrication temperature from 1450 °C to 1550 °C. The high compressive strength of graphene reinforced Nb/Nb5Si3 composites is attributed to the load transfer of graphene, and this load transfer efficiency is strengthened due to the anchoring effect of the interfacial nano-scale Nb4C3 phase. The high fracture toughness of this composite is attributed to the pull-out of graphene, as well as the crack deflection and crack bridging induced by graphene. The laminated architecture is also beneficial to improve fracture toughness because it can promote the minor deflection of cracks. The superior combination of strength and toughness of graphene reinforced Nb/Nb5Si3 composites validates that our design strategy can successfully break through the strength-toughness trade-off. This study is expected to offer a new strategy and theoretical basis for the development of Nb/Nb5Si3 composites with outstanding mechanical properties.

Abstract Growing evidence indicates that the epithelial to mesenchymal (E/M) hybrid state plays a key role in tumorigenesis. Importantly, a hybrid mesenchymal to epithelial transition (MET) state in which individual cells express both epithelial and mesenchymal markers was recently identified in vivo, further strengthening the bonds between the hybrid EMT state and cancer progression. However, the role and the molecular mechanisms by which the hybrid MET state is maintained in triple-negative breast cancer cells (TNBC) remain elusive. Here, we find that loss of ZHX2 expression results in the hybrid MET phenotype in mesenchymal TNBC cells. Mechanistically, through directly binding to the CDH1 promoter, depletion of ZHX2 specifically reactivates expression of CDH1 encoding E-cadherin, an epithelial marker that is crucial for maintaining epithelial phenotype. Functionally, loss of ZHX2 expression enriches the hybrid MET cells and inhibits the migration and dissemination of TNBC cells or organoids, which could be reversed by restoration of E-cadherin. Moreover, depletion of ZHX2 suppresses lung metastasis in preclinical models of TNBC. In patients with TNBC, ZHX2 expression was amplified and negatively correlated with the expression of E-cadherin. These findings suggest that loss of ZHX2 promotes the hybrid MET state to impair TNBC progression.

Abstract Background Acute kidney disease (AKD) has received increasing attention owing to its poor prognosis. However, few studies have investigated the trends in AKD incidence and prognosis among patients undergoing coronary angiography (CAG). Therefore, this study was designed to explore trends in the incidence and mortality of AKD following CAG in China. Methods We enrolled patients undergoing CAG using data of the Cardiorenal Improvement (CIN) cohort registry of Guangdong Provincial People's Hospital. Univariate and multivariate logistic regression analyses were applied to examine the incidence of AKD from 2008 to 2017. Mortality rates are presented as deaths per 1000 person‐years and were evaluated in univariate and multivariate Cox regression analyses. Results A total of 9665 patients (mean age: 61.6 ± 9.6 years, 40% women) were enrolled in the final analysis. From 2008 to 2017, the overall incidence and mortality of AKD were 19% and 323.0 per 1000 person‐years, respectively. The incidence of AKD decreased from 25% to 13% during 2008–2017 (adjusted odds ratio, 0.40; 95% confidence interval [CI], 0.31–0.51; p

Abstract Non-aqueous sodium-ion batteries (SiBs) are a viable electrochemical energy storage system for grid storage. However, the practical development of SiBs is hindered mainly by the sluggish kinetics and interfacial instability of positive-electrode active materials, such as polyanion-type iron-based sulfates, at high voltage. Here, to circumvent these issues, we proposed the multiscale interface engineering of Na2.26Fe1.87(SO4)3, where bulk heterostructure and exposed crystal plane were tuned to improve the Na-ion storage performance. Physicochemical characterizations and theoretical calculations suggested that the heterostructure of Na6Fe(SO4)4 phase facilitated ionic kinetics by densifying Na-ion migration channels and lowering energy barriers. The (11-2) plane of Na2.26Fe1.87(SO4)3 promoted the adsorption of the electrolyte solution ClO4 − anions and fluoroethylene carbonate molecules, which formed an inorganic-rich Na-ion conductive interphase at the positive electrode. When tested in combination with a presodiated FeS/carbon-based negative electrode in laboratory- scale single-layer pouch cell configuration, the Na2.26Fe1.87(SO4)3-based positive electrode enables an initial discharge capacity of about 83.9 mAh g−1, an average cell discharge voltage of 2.35 V and a specific capacity retention of around 97% after 40 cycles at 24 mA g−1 and 25 °C.

Magnetically coupled resonant technology is a novel method for solving the breakpoint locating of power grounding grid. But the method can only detect breakpoints of a single mesh grounding grid at present. In this paper, a magnetically coupled resonant detection method for four-hole grounding grid breakpoint is proposed. Firstly, the equivalent circuit model of the four mesh grounding grid with two types of breakpoints, namely edge branch and intermediate branch, is established. The input impedance and phase angle of the system are obtained by analyzing the equivalent capacitance and equivalent resistance in the model. Secondly, the magnetically coupled resonant physical process of grounding grid faults is solved via HFSS software. The magnetic field intensity and phase frequency characteristic curves of four mesh holes with different branches and positions of breakpoints and different corrosion degrees are studied, and an experimental system is built to verify the feasibility. The results show that under the condition of grounding grid buried depth of 0.5 m and input frequency of 1∼15MHz, and there is an inverse relationship between equivalent capacitance and distortion frequency, the phase angle is positively correlated with the degree of corrosion of grounding grid, and the error of signal distortion frequency can be positioned at 5%. This paper provides some ideas for the application of magnetic coupling grounding grid detection technology.

Abstract Given the leveling off in oxidized nitrogen emissions around the world, the atmospheric deposition of reduced nitrogen (NH x = NH3 + NH4 +) has become progressively critical, especially dry deposition, which presents great threats to plant growth. A combination of historical deposition data of measured wet NH x and modeled dry NH x in China suggests that dry NHx deposition has been increasing substantially (4.50% yr−1, p

AbstractAcute kidney injury (AKI) occurred in 12.8% of patients undergoing surgery and is associated with increased mortality. Chronic kidney disease (CKD) is a well-known risk for death and cardiovascular disease (CVD). Effects of AKI and CKD on patients undergoing coronary angiography (CAG) remain incompletely defined. The aim of our study was to investigate the relationship between acute and CKD and mortality in patients undergoing CAG. The cohort study included 49,194 patients in the multicenter cohort from January 2007 to December 2018. Cox regression analyses and Fine-Gray proportional subdistribution risk regression analysis are used to examine the association between kidney disease and all-cause and cardiovascular mortality. In the present study, 13,989 (28.4%) patients had kidney disease. During follow-up, 6144 patients died, of which 4508 (73.4%) were due to CVD. AKI without CKD (HR: 1.54, 95% CI: 1.36–1.74), CKD without AKI (HR: 2.02, 95% CI: 1.88–2.17), AKI with CKD (HR: 3.26, 95% CI: 2.90–3.66), and end-stage kidney disease (ESKD; HR: 5.63, 95% CI: 4.40–7.20) were significantly associated with all-cause mortality. Adjusted HR (95% CIs) for cardiovascular mortality was significantly elevated among patients with AKI without CKD (1.78 [1.54–2.06]), CKD without AKI (2.28 [2.09–2.49]), AKI with CKD (3.99 [3.47–4.59]), and ESKD (6.46 [4.93–8.46]). In conclusion, this study shows that acute or CKD is present in up to one-third of patients undergoing CAG and is associated with a substantially increased mortality. These findings highlight the importance of perioperative management of kidney function, especially in patients with CKD.Impact StatementWhat is already known on this subject? Acute kidney injury (AKI) occurred in 12.8% of patients undergoing surgery and is linked to a 22.2% increase in mortality. Chronic kidney disease (CKD) is a well-known risk for death and cardiovascular events. Effects of AKI and CKD on patients undergoing coronary angiography (CAG) remain incompletely defined.What do the results of this study add? This study shows that kidney disease is present in up to one-third of patients undergoing CAG and is associated with a substantially increased mortality. AKI and CKD are independent predicators for mortality in patients undergoing CAG.What are the implications of these findings for clinical practice and/or further research? These findings highlight the importance of perioperative management of kidney function, especially in patients with CKD.

Abstract With the power conversion efficiency of binary polymer solar cells dramatically improved, the thermal stability of the small-molecule acceptors raised the main concerns on the device operating stability. Here, to address this issue, thiophene-dicarboxylate spacer tethered small-molecule acceptors are designed, and their molecular geometries are further regulated via the thiophene-core isomerism engineering, affording dimeric TDY-α with a 2, 5-substitution and TDY-β with 3, 4-substitution on the core. It shows that TDY-α processes a higher glass transition temperature, better crystallinity relative to its individual small-molecule acceptor segment and isomeric counterpart of TDY-β, and a more stable morphology with the polymer donor. As a result, the TDY-α based device delivers a higher device efficiency of 18.1%, and most important, achieves an extrapolated lifetime of about 35000 hours that retaining 80% of their initial efficiency. Our result suggests that with proper geometry design, the tethered small-molecule acceptors can achieve both high device efficiency and operating stability.

Abstract Background A lthough the triglyceride-glucose (TyG) index has been shown to closely correlate with cardiometabolic outcomes and predict cardiovascular events in many groups, it remains unclear whether obese status in young and middle-aged adults is associated with long-term unfavorable cardiovascular events. This warrants further investigation. Methods This retrospective cohort study analyzed data from the National Health and Nutrition Examination Survey spanning the years 1999–2018, with follow-up for mortality status until December 31, 2019. To categorize participants based on the TyG level, the optimal critical value was determined through restricted cubic spline function analysis, dividing them into high and low TyG groups. The study assessed the relationship between TyG and cardiovascular events and all-cause mortality in young and middle-aged adults stratified by obesity status. Kaplan‒Meier and Cox proportional risk models were used to analyze the data. Results During a follow-up period of 123 months, a high TyG index increased the risk of cardiovascular events by 63% (P = 0.040) and the risk of all-cause mortality by 32% (P = 0.010) in individuals after adjusting for all covariates. High TyG was shown to be linked to cardiovascular events in obese people (Model 3: HR = 2.42, 95% CI = 1.13–5.12, P = 0.020); however, there was no significant difference in TyG groups for nonobese adults in Model 3 (P = 0.08). Conclusions TyG was independently associated with harmful long-term cardiovascular events in young and middle-aged US populations, with a stronger association observed in those who were obese.

The combined effect of Sn and pre-aging on hardening and precipitation behavior of Al-1.0Mg-0.6Si(-0.3Cu) alloy was investigated by using hardness measurement, tensile test, transmission electron microscopy (TEM) and differential scanning calorimetry (DSC). The results indicated that the natural aging (NA) hardening of Al-1.0Mg-0.6Si (−0.3Cu) alloy was suppressed by pre-aging treatment, and the suppression of NA can be enhanced by the addition of Cu. However, the suppression of NA time of Al-1.0Mg-0.6Si and Al-1.0Mg-0.6Si-0.3Cu alloy, i.e., without obvious hardness increase after pre-aging treatment, is reduced to 5 and 7 days by the addition of Sn, which is not conducive to the suppression of NA. For subsequent artificial aging (AA) of the Al-1.0Mg-0.6Si (−0.3Cu) alloys, the AA precipitation of Al-1.0Mg-0.6Si alloy with pre-aging treatment is accelerated by the addition of Sn or Cu, particularly by adding both Sn and Cu, resulting in the increase in the bake hardening response. The effect of the evolution of clusters after pre-aging on underling mechanisms of NA and its AA were also discussed.

With the development of urbanization, more and more construction and demolition waste (CDW) is generated. To enhance the mechanical properties and durability of concrete through the incorporation of recycled aggregate, the water/cement ratio was controlled to optimize the properties of concrete. In this work, one reference concrete with a water/cement ratio of 0.5 was prepared. The demolition concrete waste from East China was used, and 50% and 100% of the natural aggregates of the reference concrete were substituted. Furthermore, the water/cement ratio of concrete with 50% and 100% CDW was reduced to 0.3, and the superplasticizer was used to justify the workability of fresh concrete. Finally, the workability of fresh concrete was determined. After curing for 28 days, the density, water absorption, and resistance to chloride penetration of concrete were realized. The compressive and flexural strength were examined at 14 and 28 days, and the electrical resistivity test was conducted at 7, 14, and 28 days. The results indicate that with increasing CDW content, the mechanical properties and durability of concrete decreased. However, when the water/cement ratio decreased to 0.3, the concrete properties were optimized, such as the compressive strength and resistance to chloride penetration of concrete with 50% CDW increased by 74.2% and 28%, respectively.

Utilizing panel data pertaining to green building across 333 prefecture-level administrative units in China (excluding Hong Kong, Macao, and Taiwan) during the period spanning 2008–2020, an exhaustive examination of the evolution of China’s spatial pattern in green building is conducted employing the nearest neighbor index method, spatial autocorrelation analysis method, and kernel density analysis method. Furthermore, geographic probes are employed to scrutinize the determinants influencing China’s spatial configuration of green buildings. The findings reveal that: (1) An alteration in the density distribution from a “unipolar nucleus and double sub-nuclei” configuration to a “triple polar nuclei and multiple sub-nuclei” manifestation has been discerned in the spatial agglomeration of green buildings in China, exhibiting annual growth. Additionally, the center of green building development has shifted from the northwest to the southwest. (2) Pronounced agglomerations are predominantly situated in the eastern, central, and western regions of the country. High-high agglomerations have gradually dissipated over time in the central provincial capitals of China, the Yangtze River Delta, the Pearl River Delta, and the city clusters of Beijing-Tianjin-Hebei along the eastern seaboard. The western regions manifest a concentration of low-low and low-high aggregates, with high-low agglomeration primarily observed in the provincial capitals of the western regions. (3) The spatial differentiation of green buildings in China is attributable to a multitude of variables encompassing the environment, economy, society, and policies. Among these factors, economic, social, and innovative elements exert the most significant influence on the explicable degree of spatial differentiation.

The limestone reservoir of the Da’anzhai section is the main production layer of Jurassic tight oil in central Sichuan, with tight and strongly heterogeneous reservoir rocks. In the early stages, small-scale plug removal and acidification techniques were mainly applied to the dacite section of the reservoir. However, using a single conversion method was not suitable for tight reservoirs with underdeveloped fractures. To address this challenge, we conducted large-scale real-world triaxial simulation test system experiments on hydraulic fracture propagation using samples from dense limestone outcrops. The aim was to elucidate the laws of hydraulic fracture propagation in dense limestone oil reservoirs. The L1 well in this area was selected to test the volumetric fracturing technology in order to achieve a breakthrough in test production and the reconstruction technology of the Da’anzhai section of the tight limestone reservoir. The experiments have shown that low stress differences, natural fracture development, and low viscosity slippery water are favorable factors for obtaining complex fracture morphology. In contrast to the construction parameters, the structural properties of the rock are the dominant factors in the formation of complex fracture morphologies. By optimizing the geological dessert and implementing targeted technological measures, bulk fracturing has been proven to be feasible for the dense limestone reservoir in the Da’anzhai section.

Butylparaben (BuP) is a common antibacterial preservative utilized extensively in food, medical supplies, cosmetics, and personal care products. The current study reports the use of Zebrafish (Danio rerio) embryos to investigate potential developmental toxicity caused by exposure to BuP. The development of Neural crest cells (NCCs) is highly active during gastrulation in Zebrafish embryos. Thus, we utilized 0.5 mg/L, 0.75 mg/L, and 1 mg/L BuP solutions, respectively, in accordance with the international safety standard dosage. We observed severe craniofacial cartilage deformities, periocular edema, cardiac dysplasia, and delayed otolith development in the Zebrafish larvae 5 days after exposure. The oxidative stress response was significantly enhanced. In addition, the biochemical analysis revealed that the activities of catalase (CAT) and superoxide dismutase (SOD) were significantly reduced relative to the control group, whereas the concentration of malondialdehyde (MDA) was significantly elevated. Furthermore, ALP activity, a marker of osteoblast activity, was also reduced. Moreover, the RT-qPCR results indicated that the expression of chondrocyte marker genes sox9a, sox9b, and col2a1a was down-regulated. In addition, the morphology of maxillofacial chondrocytes was altered in Zebrafish larvae, and the proliferation of cranial NCCs was inhibited. Accordingly, our findings indicate that strong oxidative stress induced by BuP inhibits the proliferation of NCCs in larval Zebrafish, leading to craniofacial deformities.

Objective: Deleted in liver cancer 1 (DLC1) is a GTPase-activating protein that is reported as a suppressor in certain human cancers. However, the detailed biological function of DLC1 is still unclear in human prostate cancer (PCa). In the present study, we aimed to explore the function of DLC1 in PCa cells. Methods: Silencing and overexpression of DLC1 were induced in an androgen-sensitive PCa cell line (LNCaP) using RNA interference and lentiviral vector transduction. The Cell Counting Kit-8 assay was performed to determine cell proliferation. The cell cycle was examined by performing a propidium iodide staining assay. Results: Our results indicated that DLC1 overexpression markedly suppressed the proliferation and cell cycle progression of LNCaP cells. Moreover, DLC1 expression was negatively correlated with Rho-associated protein kinase (ROCK) expression in LNCaP cells. Importantly, this study showed that the ROCK inhibitor Y27632 restored the function of DLC1 in LNCaP cells and reduced the tumorigenicity of LNCaP cells in vivo. Conclusion: Our results indicated that DLC1 overexpression markedly suppressed the proliferation and cell cycle progression of PCa cells and negatively correlated with ROCK expression in PCa cells and tissue.

Abstract Background The triglyceride-glucose (TyG) index is a reliable alternative biomarker of insulin resistance (IR). However, whether the TyG index has prognostic value in critically ill patients with coronary heart disease (CHD) remains unclear. Methods Participants from the Medical Information Mart for Intensive Care III (MIMIC-III) were grouped into quartiles according to the TyG index. The primary outcome was in-hospital all-cause mortality. Cox proportional hazards models were constructed to examine the association between TyG index and all-cause mortality in critically ill patients with CHD. A restricted cubic splines model was used to examine the associations between the TyG index and outcomes. Results A total of 1,618 patients (65.14% men) were included. The hospital mortality and intensive care unit (ICU) mortality rate were 9.64% and 7.60%, respectively. Multivariable Cox proportional hazards analyses indicated that the TyG index was independently associated with an elevated risk of hospital mortality (HR, 1.71 [95% CI 1.25–2.33] P = 0.001) and ICU mortality (HR, 1.50 [95% CI 1.07–2.10] P = 0.019). The restricted cubic splines regression model revealed that the risk of hospital mortality and ICU mortality increased linearly with increasing TyG index (P for non-linearity = 0.467 and P for non-linearity = 0.764). Conclusions The TyG index was a strong independent predictor of greater mortality in critically ill patients with CHD. Larger prospective studies are required to confirm these findings.

As a promising cathode material, Na3V2(PO4)2F3 (NVPF) has attracted wide attention for sodium-ion batteries (SIBs) because of its high operating voltage and high structural stability. However, the low intrinsic electronic conductivity and insufficient Na ion mobility of NVPF limit its development. Herein, K-doping NVPF is prepared through a facile ball-milling combined calcination method. The effects of K-doping on the crystal structure, kinetic properties and electrochemical performance are investigated. The results demonstrate that the Na2.90K0.10V2(PO4)3F3 (K0.10-NVPF) exhibits a high capacity (120.8 mAh g−1 at 0.1 C), high rate capability (66 mAh g−1 at 30 C) and excellent cycling performance (a capacity retention of 97.5% at 1 C over 500 cycles). Also, the occupation site of K ions in the lattice, electronic band structure and Na-ion transport kinetic property in K-doped NVPF are investigated by density functional theory (DFT) calculations, which reveals that the K-doped NVPF exhibits improved electronic and ionic conductivities, and located K+ ions in the lattice to contribute to high reversible capacity, rate capability and cycling stability. Therefore, the K-doped NVPF serves as a promising cathode material for high-energy and high-power SIBs.

The CoCrxCuFeMnNiV (x = 0, 0.25, 0.5, 0.75, 1) high-entropy alloy was prepared via vacuum arc smelting, and the effect of different Cr contents on the microstructure evolution and mechanical properties of it was evaluated. The results show that the CoCuFeMnNiV alloy is composed of FCC1+FCC2 phases, while Cr-added CoCrxCuFeMnNiV alloy is composed of FCC1+FCC2+sigma phases; moreover, the amount of sigma phase increases with the Cr content. Through calculations, a phase formation criterion suitable for the CoCrxCuFeMnNiV alloy was established. It was observed that adding Cr does not affect the early solidification behaviour of the FCC1 and FCC2 phases; however, it affects the stability of the FCC1 phase and promotes the transformation of this phase into the sigma phase, as well as secondary precipitation of a nanoscale copper-rich phase on this phase. The formation of sigma phase has a significant strengthening effect on the alloy but reduces its plasticity. The CoCr0.5CuFeMnNiV alloy has favourable comprehensive properties; specifically, its yield strength, compressive strength, microhardness, and fracture strain are 820 MPa, 1050 MPa, 320 HV, and 22.5%, respectively. With an increase in Cr content, the alloy fracture mode changes from ductile to brittle.

Abstract Background Red blood cell distribution width (RDW) is highly associated with adverse clinical outcomes in many diseases. The present study aimed to evaluate the relationship between RDW and gastrointestinal bleeding (GIB) after isolated coronary artery bypass grafting (CABG). Methods This was a retrospective observational study that included 4473 patients who received CABG, and all the data were extracted from the Medical Information Mart for Intensive Care III database. Data collected included patient demographics, associated comorbid illnesses, laboratory parameters, and medications. The receiver operating characteristic (ROC) curve was used to determine the best cutoff value of RDW for the diagnosis of GIB. Multivariable logistic regression analysis was used to analyze the relationship between RDW and GIB. Results The incidence of GIB in patients receiving CABG was 1.1%. Quartile analyses showed a significant increase in GIB incidence at the fourth RDW quartile (> 14.3%; P 14.1% measured on admission had 59.6% sensitivity and 69.4% specificity in predicting GIB after CABG. After adjustment for confounders, high RDW was still associated with an increased risk of GIB in patients with CABG (odds ratio = 2.83, 95% confidence interval 1.46–5.51, P = 0.002). Conclusions Our study indicates that the elevated RDW level is associated with an increased risk of GIB after CABG, and it can be an independent predictor of GIB. The introduction of RDW to study GIB enriches the diagnosis method of GIB and ensures the rapid and accurate diagnosis of GIB.

Abstract Background Despite many significant advances in treatment and management, cardiovascular disease remains the main cause of the global disease burden. Nutrition-related disease is a modifiable cardiovascular risk factor. However, few studies have examined the relationship between nutrition-related diseases and cardiovascular mortality. Objective We aimed to investigate the association of nutrition-related diseases with cardiovascular mortality based on a large nationally representative community population. Design We analyzed data from the National Health and Nutrition Examination Survey (NHANES) 1999–2006 with mortality follow-up through December 31, 2015. Finally, 12,469 participants were analyzed. Each participant was assigned to one of four groups: normal nutrition without sarcopenia, sarcopenia with normal nutrition, malnutrition without sarcopenia, and malnutrition-sarcopenia syndrome. Survival curves and Cox regressions based on the NHANES recommended weights were used to assess the association between nutrition-related diseases and cardiovascular mortality. Results Of the 12,469 patients included in the study and divided into four groups, malnutrition-sarcopenia syndrome had the highest 5- and 10-year cardiovascular mortality rates. After adjustment for related factors, sarcopenia with normal nutrition (hazard ratio [HR]: 1.62, 95% confidence interval [CI]: 1.28–2.06; P

ObjectiveInflammation plays an important role in the pathophysiology of hypertension (HTN). Aggregate index of systemic inflammation (AISI), as a new inflammatory and prognostic marker has emerged recently. Our goal was to determine whether there was a relationship between HTN and AISI.MethodsWe analyzed patients with HTN from the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2018. The primary end point was cardiovascular mortality. A total of 23,765 participants were divided into four groups according to the AISI quartile level. The association between AISI and cardiovascular mortality in patients with HTN was assessed by survival curves and Cox regression analyses based on NHANES recommended weights.ResultsHigh levels of AISI were significantly associated with cardiovascular mortality in patients with HTN. After full adjustment for confounders, there was no significant difference in the risk of cardiovascular mortality in Q2 and Q3 compared to Q1, while Q4 (HR: 1.91, 95% CI: 1.42–2.58; P

The burial depth of Penglai gas field reservoir in the Sichuan Basin of China exceeds 7,000 m, and the reservoir temperature is 160°C. Penglai gas field belongs to the fractured reservoir, and there are many natural fractures distributed in the reservoir. Natural fractures are not only the storage place of natural gas, but also the transportation channel of natural gas. Gas wells mainly increase natural gas production through acid fracturing. In the early stage, Penglai Gas Field mainly used gelled acid fracturing technology to create an artificial fracture in the reservoir by injecting a large amount of acid fluid. However, the stimulation range of gelled acid fracturing is very small. After acid fracturing, gas wells can only produce 120,000 cubic meters of natural gas per day. To obtain higher natural gas production, this paper proposes a multi-stage temporary acid fracturing technology that can greatly improve the effect of acid fracturing. The temporary plugging agent for acid fracturing has been developed to meet the high-temperature requirements of the Penglai gas field. Based on the simulation of fracture propagation, the feasibility of acid fracturing expansion of natural fractures in the Penglai Gas Field is clarified. Fiber and granular temporary plugging agents that meet the plugging strength greater than 20 MPa are selected by using the dynamic temporary plugging instrument. MultiFracS software is used to optimize the multi-stage temporary acid fracturing process parameters. The research results in this paper have been applied to wells PS101, PS102, and PS103. After fracturing, compared with the gelling acid fracturing, the natural gas production has increased by more than three times, and the multi-stage temporary plugging acid fracturing has achieved a very good stimulation effect.

Article highlights According to the characteristics of dispersed and superimposed development of high-quality reservoirs in long horizontal section, the acid fracturing process criterion of "one section and one policy" was established. The acid fracturing process criteria for reservoirs with different transformation potential are established, and the pertinence of acid fracturing transformation is improved. Gel acid fracturing process is adopted for high-quality reservoir, the acid fracturing process of authigenic acid preflush is adopted for medium reservoir and the acid fracturing process of multistage alternating injection of authigenic acid preflush + gelled acid is adopted for low-quality reservoirs. The targeted reconstruction of strong heterogeneity long well section is realized.

Abstract The need for large‐scale electrochemical energy storage devices in the future has spawned several new breeds of batteries in which aqueous zinc ion batteries (AZIBs) have attracted great attention due to their high safety, low cost, and excellent electrochemical performance. In the current research, the dendrite and corrosion caused by aqueous electrolytes are the main problems being studied. However, the research on the zinc metal anode is still in its infancy. We think it really needs to provide clear guidelines about how to reasonably configure the system of AZIBs to realize high‐energy density and long cycle life. Therefore, it is worth analyzing the works on the zinc anode, and several strategies are proposed to improve the stability and cycle life of the battery in recent years. Based on the crystal chemistry and interface chemistry, this review reveals the key factors and essential causes that inhibit dendrite growth and side reactions and puts forward the potential prospects for future work in this direction. It is foreseeable that guiding the construction of AZIBs with high‐energy density and long cycle life in various systems would be quite possible by following this overview as a roadmap.

The acoustic radiation characteristics of pipeline structures caused by turbulent fluctuating pressure are among the most important acoustic radiation sources in vessel cabins. Studying the vibro-acoustic characteristics of pipeline structures is of great relevance to engineering. The turbulence fluctuation pressure of pipeline structures’ inner surfaces was numerically analyzed by using the large eddy simulation (LES) method. At first, the vibro-acoustic differential equation of the pipeline structure system was derived by defining the fluctuation pressure as the excitation loading. According to the acoustic radiation characteristic in the quarter-infinite space, which had a combination of two rigid wall interfaces at the same time for the pipeline structures in the vessel cabins, the double reflection method, mirror image method and elastic wave Graf addition principle were applied, and the analytical function of the acoustic radiation for the cylindrical shell was derived. For the pipeline structures in the quarter-infinite acoustic space, for example, the numerical calculation for the acoustic radiation of cylindrical shell was carried out, which was excited by the turbulent pulsating pressure. Finally, the influence of inner flow velocity, frequency, and pipeline installation position were compared using numerical analysis. The results can provide technical support for the acoustic design of pipeline structure systems with complex acoustic boundaries.

Continuous fiber-reinforced thermoplastic composites (CFRTPCs) have advantages such as high specific strength, high specific modulus, corrosion resistance, and recyclability and are widely used in the fields of aerospace, rail transit, new energy, and so on. However, traditional methods for preparing CFRTPCs, such as placement and molding, rely more on forming molds, resulting in high manufacturing costs and a slow response speed, which limits the promotion and application of the new generation of CFRTPCs with complex configurations and designable performance. Three-dimensional printing can efficiently create products with multiple materials, complex structures, and integrated functions, introducing new ways and opportunities for the manufacturing of CFRTPCs. However, poor mechanical properties are the bottleneck problem in achieving 3D printing of CFRTPCs. This paper summarizes the research status of the fused deposition modeling (FDM) 3D printing process and the corresponding mechanical properties of CFRTPCs. The focus is on analyzing the influences of the FDM process parameters, such as the material type, printing temperature, speed parameters, layer thickness, scanning space, stacking direction, and fiber volume content, on the mechanical properties of CFRTPCs. Finally, the main problems and future prospects of current CFRTPCs-FDM are analyzed and forecasted, providing new references and ideas for 3D printing of high-performance CFRTPCs.

Magneto-acoustic concentration tomography of magnetic nanoparticles (MNPs) with magnetic induction (MACT-MI) is an emerging method for magnetic particle imaging based on the magneto-acoustic coupling effect. However, the limitation of this method is that the concentration of magnetic nanoparticles in the human body should be regulated to prevent excessive metabolic pressure on the kidneys. Therefore, on the premise of keeping the concentration constant, the magnetic calculation method of MNPs under the relaxation mechanism is specifically studied. We deduce the optimal particle size for imaging and greatly increased the magnetic force and sound pressure of MACT-MI, which is more conducive to the acquisition of magneto-acoustic signals. In addition, rationally increasing the fluid ambient temperature and selecting the diameter of magnetic nanoparticles according to different target tissue fluids can also optimize the imaging effect. This research provides a technical basis for the application of MNPs in tumor markers and targeted drug release.

The traditional manual periodic screening method of grading electrode sediments is prone to cause the equipment failure of high voltage direct current converter valves. Therefore, we propose to use ultrasonic time-domain reflection method to detect the sediments. However, the ultrasonic echo signals are characterized by nonlinearity and nonsmoothness, which makes it very difficult to extract effective features for sediment detection. To address this issue, we propose an intelligent detection method based on multiscale hybrid entropy characteristics in the time-frequency domain. First, a multiscale decomposition of the signal is performed. Second, the weighted form factor index is proposed to select the noise modes. Moreover, we propose to calculate the hybrid entropy in the time-frequency domain of each mode as the characteristic input bidirectional long and short-term memory network model, and verified that feature enhancement can be achieved by noise modes noise reduction. Finally, the experimental validation shows that the proposed method can achieve nondestructive testing and intelligent identification of graded electrode sediment with a correct identification rate of 94.25%.

Multi-cluster horizontal well fracturing is one of the key technologies to develop the unconventional reservoirs such as shales. However, the field data shows that some perforation clusters have little production contribution. In this study, a three-dimensional (3D) numerical model for simulating the multiple fracture propagation based on 3D block discrete element method was established, and this model considers the stress interference, perforation friction and fluid-mechanical coupling effect. In order to determine the most appropriate measures to improve the uniformity of multiple fracture propagation, the effect of the geologic and engineering parameters on the multiple fracture propagation in shale reservoirs is investigated. The modeling results show that the geometry of each fracture within a stage is different, and the outer fractures generally receive more fracturing fluid than the interior fractures. The vertical stress almost has no effect on the geometries of multiple fractures. However, higher horizontal stress difference, larger cluster spacing, smaller perforation number, higher injection rate, and smaller fracturing fluid viscosity are conducive to promote the uniform propagation of multiple fractures. The existence of bedding planes will increase the fluid filtration, resulting in a reduction in fracture length. The middle two fractures receive less fluid and the width of them is smaller. Through analyzing the numerical results, a large amount of fracturing fluid should be injected and the proppant with smaller size is suggested to be used to effectively prop the bedding planes. Cluster spacing and perforation number should be controlled in an appropriate range according to reservoir properties. Increasing the injection rate and reducing the viscosity of fracturing fluid are important means to improve the geometry of each fracture.

IntroductionEngineering membrane transporters to achieve desired functionality is reliant on availability of experimental data informing structure-function relationships and intelligent design. Plant aquaporin (AQP) isoforms are capable of transporting diverse substrates such as signaling molecules, nutrients, metalloids, and gases, as well as water. AQPs can act as multifunctional channels and their transport function is reliant on many factors, with few studies having assessed transport function of specific isoforms for multiple substrates.MethodsHigh-throughput yeast assays were developed to screen for transport function of plant AQPs, providing a platform for fast data generation and cataloguing of substrate transport profiles. We applied our high-throughput growth-based yeast assays to screen all 13 Arabidopsis PIPs (AtPIPs) for transport of water and several neutral solutes: hydrogen peroxide (H2O2), boric acid (BA), and urea. Sodium (Na+) transport was assessed using elemental analysis techniques.ResultsAll AtPIPs facilitated water and H2O2 transport, although their growth phenotypes varied, and none were candidates for urea transport. For BA and Na+ transport, AtPIP2;2 and AtPIP2;7 were the top candidates, with yeast expressing these isoforms having the most pronounced toxicity response to BA exposure and accumulating the highest amounts of Na+. Linking putative AtPIP isoform substrate transport profiles with phylogenetics and gene expression data, enabled us to align possible substrate preferences with known and hypothesized biological roles of AtPIPs.DiscussionThis testing framework enables efficient cataloguing of putative transport functionality of diverse AQPs at a scale that can help accelerate our understanding of AQP biology through big data approaches (e.g. association studies). The principles of the individual assays could be further adapted to test additional substrates. Data generated from this framework could inform future testing of AQP physiological roles, and address knowledge gaps in structure-function relationships to improve engineering efforts.

BackgroundPatients with Rheumatoid arthritis (RA) are prone to malnutrition. However, it is rare studies assessing the relationship between malnutrition and all-cause mortality in patients with RA.ObjectiveTo investigate the relationship between malnutrition and all-cause mortality in patients with RA in a large national sample cohort.MethodsWe analyzed data on 1,976 adults ≥ 18 years of age during National Health and Nutrition Examination Survey (NHANES) 1999–2014. We chose the Controlled Nutritional Status Score (CONUT) and the Nutritional Risk Index (NRI) to assess the nutritional status of patients with RA. The Kaplan–Meier (KM) survival curves Cox proportional hazards regression models were used to analyze the associations between malnutrition and all-cause mortality.ResultsOf the 1,976 patients with RA (57.38 ± 0.40 years, female 59.9%, non-Hispanic white 69.9%), the prevalence of malnutrition was 18.8% by used the CONUT and 26.6% by used the NRI. The KM survival curves showed that malnutrition was associated with a higher incidence of all-cause mortality during the 10-year follow-up period (log-rank test, P < 0.001). In the fully corrected model, the adjusting hazard ratio (aHR) for all-cause mortality in patients with moderate to severe malnutrition with CONUT and NRI were 5.63 (95% CI, 2.55–12.45; P < 0.001) and 2.56 (95% CI, 1.81–3.62; P < 0.001), respectively, compared with patients without malnutrition.ConclusionMalnutrition is very prevalent in patients with RA, approximately 18.8% (CONUT) to 26.6% (NRI). Malnutrition is strongly associated with an increased risk of all-cause mortality. These findings underscore the importance of attention and intervention in the nutritional status of patients with RA. Further clinical trials are needed to prospectively assess the effect of nutritional interventions on the prognosis of patients with RA.

Abstract Background The platelet-to-hemoglobin ratio (PHR) has emerged as a prognostic biomarker in coronary artery disease (CAD) patients after PCI but not clear in CAD complicated with congestive heart failure (CHF). Hence, we aimed to assess the association between PHR and long-term all-cause mortality among CAD patients with CHF. Methods Based on the registry at Guangdong Provincial People’s Hospital in China, we analyzed data of 2599 hospitalized patients who underwent coronary angiography (CAG) and were diagnosed with CAD complicated by CHF from January 2007 to December 2018. Low PHR was defined as ˂ 1.69 (group 1) and high PHR as ≥ 1.69 (group 2). Prognosis analysis was performed using Kaplan–Meier method. To assess the association between PHR and long-term all-cause mortality, a Cox-regression model was fitted. Results During a median follow-up of 5.2 (3.1–7.8) years, a total of 985 (37.9%) patients died. On the Kaplan–Meier analysis, patients in high PHR group had a worse prognosis than those in low PHR group (log-rank, p = 0.0011). After adjustment for confounders, high PHR was correlated with an increased risk of long-term all-cause mortality in CAD patients complicated with CHF. (adjusted hazard ratio [aHR], 1.31; 95% confidence interval [CI], 1.13–1.52, p

Objective The American Heart Association (AHA) proposed the concept of ideal cardiovascular health (CVH) to reduce the risk of cardiovascular mortality. We attempted to broaden the impact of CVH and further contribute to AHA 2030 goals by identifying the relationship between CVH and non-cardiovascular diseases such as sarcopenia.Design Cross-sectional surveySetting National Health and Nutrition Examination Survey conducted in the USA from 2011 to 2018.Participants This study included participants with reliable first 24-hour dietary recall and ≥20 years of age and excluded those who could not diagnose sarcopenia or insufficient data to calculate the CVH scores.Primary and secondary outcome measures The prevalence of sarcopenia as measured by dual-energy X-ray absorptiometry.Results This cohort study involving 9326 adults≥20 years comprised 4733 females (50.0%). The number of intermediate or ideal and poor CVH participants was 5654 and 3672 with mean CVH score of 9.70±0.03 and 5.66±0.04, respectively. After adjusting for related confounding factors, intermediate or ideal CVH was associated with an odds reduction of sarcopenia than poor CVH (adjusted OR (aOR): 0.36, 95% CI 0.26 to 0.50, p5, the odds of sarcopenia decreased by up to 84% (aOR: 0.16, 95% CI 0.08 to 0.30).Conclusions Our findings suggest a relationship between the CVH and the prevalence of sarcopenia in adults. The results of our study can contribute to achieving the 2030 public health goal of achieving CVH for all, which may be supported by efforts to reduce the prevalence of sarcopenia.

The health status of equipment is of paramount importance during the operation of nuclear power plants. The occurrence of faults not only leads to significant economic losses but also poses risks of casualties and even major accidents, with unimaginable consequences. This paper proposed a deep learning framework called PT-Informer for fault prediction, detection, and localization in order to address the challenges of online monitoring of the operating health of nuclear steam turbines. Unlike traditional approaches that involve separate design and execution of feature extraction for fault diagnosis, classification, and prediction, PT-Informer aims to extract fault features from the raw vibration signal and perform ultra-real-time fault prediction prior to their occurrence. Specifically, the encoding and decoding structure in PT-Informer ensures the capture of temporal dependencies between input features, enabling accurate time series prediction. Subsequently, the predicted data are utilized for fault detection using PCA in the PT-Informer framework, aiming to assess the likelihood of equipment failure in the near future. In the event of potential future failures, t-SNE is utilized to project high-dimensional data into a lower-dimensional space, facilitating the identification of clusters or groups associated with different fault types or operational conditions, thereby achieving precise fault localization. Experimental results on a nuclear steam turbine rotor demonstrate that PT-Informer outperformed the traditional GRU with a 4.94% improvement in R2 performance for prediction. Furthermore, compared to the conventional model, the proposed PT-Informer enhanced the fault classification accuracy of the nuclear steam turbine rotor from 97.4% to 99.6%. Various comparative experiments provide strong evidence for the effectiveness of PT-Informer framework in the diagnosis and prediction of nuclear steam turbine.

BackgroundIdentifying patients with low muscle mass is crucial for the diagnosis of sarcopenia. Although the Creatinine/Cystatin C (Cr/CysC) is recommended as a simplified indicator to identify patients with low muscle mass, its ability to assess muscle mass and predict a poor prognosis has not been validated. We aimed to determine the diagnosis value of Cr/CysC for low muscle mass and examine the association of Cr/CysC with mortality.MethodsIn this cohort study we analyzed data from the National Health and Nutrition Examination Survey from 1999 to 2002. Follow-up was conducted up to December, 2015. Appendicular skeletal mass was calculated based on dual-energy X-ray absorptiometry (DXA) scans. Low muscle mass was defined referring to five international diagnostic criteria. The diagnostic value of Cr/CysC as a replacement indicator of muscle mass was measured using area under the curve, positive percent agreement, negative percent agreement and kappa. Cox proportional hazards regression models were developed to examine the association between Cr/CysC and risk of mortality.ResultsThis cohort study of 3,741 adults comprised 1,823 females (48.73%), with a weighted mean (SE) age of 44.46 (0.43) years. The positive percent agreement of Cr/CysC for the diagnosis of low muscle mass was poor (40.23–58.74%), except for Foundation of the National Institute of Health (FNIH) criteria (80.90–58.97%). But the negative percent agreement of Cr/CysC for the diagnosis of low muscle mass was high (males: 62.15–88.17%; females: 55.26–82.30%). Moreover, the risk of death was reduced by 2% per 0.01 unit increase in Cr/CysC (aHR, 0.98; 95% CI, 0.98–0.99, P < 0.001).ConclusionsCr/CysC performed well not only in identifying non-sarcopenia cases, especially when based on FNIH diagnostic criteria, but also in revealing a positive association with higher risk of mortality. The optimal cut-off values for Cr/CysC were

BackgroundRespiratory distress syndrome (RDS) is one of the leading causes of neonatal death in the neonatal intensive care unit (NICU). Previous studies have suggested that the development of neonatal RDS may be associated with inflammation and lead to organ dysfunction. The neonatal sequential organ failure assessment (nSOFA) scoring system is an operational definition of organ dysfunction, but whether it can be used to predict mortality in neonates RDS is unknown. The aim of this study was to clarify the performance of the nSOFA score in predicting mortality in patients with neonatal RDS, with the aim of broadening the clinical application of the nSOFA score.MethodsNeonates with RDS were identified from the Medical Information Mart for Intensive Care (MIMIC)-III database. Cox proportional hazards model were used to assess the association between nSOFA score and mortality. Propensity score matched analysis were used to assess the robustness of the analytical results.ResultsIn this study of 1,281 patients with RDS of which 57.2% were male, death occurred in 40 cases (3.1%). Patients with high nSOFA scores had a higher mortality rate of 10.7% compared with low nSOFA scores at 0.3%. After adjusting for confounding, multivariate Cox proportional risk analysis showed that an increase in nSOFA score was significantly associated with increased mortality in patients with RDS [adjusted Hazards Ratio (aHR): 1.48, 95% Confidence Interval (CI): 1.32–1.67; p < 0.001]. Similarly, the High nSOFA group was significantly associated with higher mortality in RDS patients (aHR: 19.35, 95% CI: 4.41–84.95; p < 0.001) compared with the low nSOFA group.ConclusionThe nSOFA score was positively associated with the risk of mortality in cases of neonatal RDS in the NICU, where its use may help clinicians to quickly and accurately identify high risk neonates and implement more aggressive intervention.