1. The venom of Habrobracon hebetor induces alterations in host metabolism.
- Author
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Kryukova NA, Yanshole LV, Zelentsova EA, Tsentalovich YP, Chertkova EA, Alekseev AA, and Glupov VV
- Subjects
- Animals, Metabolome drug effects, Magnetic Resonance Spectroscopy, Host-Parasite Interactions drug effects, Wasps physiology, Wasp Venoms metabolism, Wasp Venoms chemistry, Moths parasitology, Moths growth & development, Moths metabolism, Larva growth & development, Larva metabolism, Hemolymph metabolism, Hemolymph chemistry
- Abstract
The ability of parasitic wasps to manipulate a host's metabolism is under active investigation. Components of venom play a major role in this process. In the present work, we studied the effect of the venom of the ectoparasitic wasp Habrobracon hebetor on the metabolism of the greater wax moth host (Galleria mellonella). We identified and quantified 45 metabolites in the lymph (cell-free hemolymph) of wax moth larvae on the second day after H. hebetor venom injection, using NMR spectroscopy and liquid chromatography coupled with mass spectrometry. These metabolites included 22 amino acids, nine products of lipid metabolism (sugars, amines and alcohols) and four metabolic intermediates related to nitrogenous bases, nucleotides and nucleosides. An analysis of the larvae metabolome suggested that the venom causes suppression of the tricarboxylic acid cycle, an increase in the number of free amino acids in the lymph, an increase in the concentration of trehalose in the lymph simultaneously with a decrease in the amount of glucose, and destructive processes in the fat body tissue. Thus, this parasitoid venom not only immobilizes the prey but also modulates its metabolism, thereby providing optimal conditions for the development of larvae., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2024. Published by The Company of Biologists Ltd.)
- Published
- 2024
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