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In the past two to three decades, synthetic glycoconjugate vaccines have shown great potential in the prevention of severe infections and protection of high‐risk populations. Conjugation of synthetic oligosaccharide haptens to carrier proteins is the key step for the vaccine preparation. In this review, the conjugation methods currently used in the synthesis of glycoconjugate vaccines from synthetic/homogeneous oligosaccharide haptens are summarized with the focus on the reaction conditions (pH and sugar/protein ratio) and performance. This information can help researchers choose the appropriate conjugation methods. Further research directions toward site‐specific conjugations and fully homogeneous glycoconjugate vaccines are also discussed. [ABSTRACT FROM AUTHOR]

Traumatic dental injuries (TDIs) are a frequent challenge in pediatric dentistry, requiring a coordinated interdisciplinary approach for successful management. These injuries can affect various dental tissues and pose significant diagnostic and therapeutic challenges, particularly in young patients with developing dentition. This review aims to explore the complexities of managing TDIs in young permanent dentition, focusing on the role of orthodontics, endodontics, and other specialties in providing comprehensive care. We highlight the importance of interdisciplinary collaboration in achieving optimal functional and aesthetic outcomes, emphasizing the need for updated diagnostic tools and treatment protocols tailored to the growing child. Specific recommendations are provided for clinicians on integrating orthodontic and endodontic management in trauma cases, focusing on minimizing complications and ensuring long-term prognosis., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Objective: To evaluate the effect of childhood dental attendance pattern on self-rated oral health in middle adulthood among the British population., Methods: Data from the 1970 British Cohort Study involving participants born in England, Scotland, and Wales were used. Self-rated oral health was assessed at age 46. Childhood dental attendance patterns, derived from follow-up surveys at ages 5, 10, and 16, were classified as 'always', 'sometimes', or 'never' regular. Logistic regression was performed to estimate the effect of childhood dental attendance on adult oral health. Subgroup analyses by education level and oral hygiene practices examined potential variations across these factors., Results: This study analyzed data from 4699 participants. An association was found between childhood dental attendance pattern and self-rated oral health in middle adulthood. The odds of reporting poor oral health were higher among those with 'sometimes' and 'never' regular dental attendance patterns than 'always' regular attenders. However, this association became insignificant after adjusting for covariates. Subgroup analyses revealed that 'sometimes' and 'never' regular attendance patterns were associated with higher odds of reporting poor oral health among participants who brushed less than twice daily and those with lower parental academic qualifications., Conclusion: This study suggests that childhood dental attendance is associated with perceived adult oral health, though this relationship diminished after adjusting for covariates. However, the association persisted among those who brushed less than twice daily and had lower parental academic qualifications. It underscores the importance of both demographic factors and health-related behaviors in determining long-term oral health outcomes., (© 2025 The Author(s). Journal of Public Health Dentistry published by Wiley Periodicals LLC on behalf of American Association of Public Health Dentistry.)

Early initiation of breastfeeding (EIBF) and exclusive breastfeeding (EBF) are highly effective forms of preventive medicine in many low- and middle-income countries, including Anglophone and Francophone West African countries. Despite the proven benefits of EIBF and EBF in reducing mortality and morbidity, there is limited systematic evidence from West African countries. Hence, the aim of this systematic review and meta-analysis was to estimate the pooled prevalence of EIBF and EBF in Anglophone and Francophone West African countries. Six databases were searched for eligible studies based on inclusion criteria and a systematic review and a further meta-analysis were done. The weighted prevalence of EIBF was 51.7% (95% CI: 48.8, 54.6) in Anglophone West African countries and 45.5% (95% CI: 42.0, 48.9) in Francophone West African countries. The pooled prevalence of EBF was 41.2% (95% CI: 36.9, 45.5) in Anglophone West African countries and 30.1% (95% CI: 26.7, 33.5) in Francophone West African countries. Our study showed that the weighted average EIBF and EBF prevalence tended to be higher from studies in Anglophone West African countries than in Francophone West African countries. Despite these findings, EIBF and EBF rates overall in West African countries were suboptimal. A substantial improvement is necessary in promoting EIBF and EBF in West African countries. Programmes should target all mothers in the region no matter their colonial allegiance to achieve Sustainable Development Goals 2 and 3 by 2030., (© 2024 The Author(s). Maternal & Child Nutrition published by John Wiley & Sons Ltd.)

Reprogramming metabolic flux is a promising approach for constructing efficient microbial cell factories (MCFs) to produce chemicals. However, how to boost the transmission efficiency of metabolic flux is still challenging in complex metabolic pathways. In this study, metabolic flux is systematically reprogrammed by regulating flux size, flux direction, and flux rate to build an efficient MCF for chondroitin production. The ammoniation pool for UDP‐GalNAc synthesis and the carbonization pool for UDP‐GlcA synthesis are first enlarged to increase flux size for providing enough precursors for chondroitin biosynthesis. Then, the ammoniation pool and the carbonization pool are rematched using molecular valves to shift flux direction from cell growth to chondroitin biosynthesis. Next, the adaptability of polymerization pool with the ammoniation and carbonization pools is fine‐tuned by dynamic and static valve‐based adapters to accelerate flux rate for polymerizing UDP‐GalNAc and UDP‐GlcA to produce chondroitin. Finally, the engineered strain E. coli F51 is able to produce 9.2 g L−1 chondroitin in a 5‐L bioreactor. This strategy shown here provides a systematical approach for regulating metabolic flux in complex metabolic pathways for efficient biosynthesis of chemicals. [ABSTRACT FROM AUTHOR]

Breast milk is the perfect food during infancy. Adequate support from family and health systems can be helpful to continue breastfeeding. This study aimed to determine the status of breastfeeding challenges and its relationship with social support and socio-demographic factors. In this correlational-descriptive study, 348 breastfeeding mothers were recruited using cluster random sampling from health care centers in Tabriz, Iran in 2022. Socio-demographic, breastfeeding challenges and social support questionnaires were used for data collection. Data were analyzed for descriptive and inferential statistics (Pearson correlation tests, independent t-test, one-way ANOVA and general linear model) using SPSS version 16. Difficulty in completing household tasks and breastfeeding at the same time (32.5 percent) was the most common challenge reported by mothers. There was an inverse and significant correlation between perceived social support and experiencing challenges (r = −0.199؛ p =.001). Based on the adjusted general linear model, with increasing social support, the score of breastfeeding challenges decreased (B = −0.165; 95 percent CI: −0.07–0.25, p <.001). Considering the relationship between perceived social support and the challenges experienced during breastfeeding, it can be concluded that adequate support from family along with training and guidance from health care providers can lead women to have better breastfeeding experiences and overcome breastfeeding problems. [ABSTRACT FROM AUTHOR]

The fusion peptide (FP) on the HIV-1 envelope (Env) trimer can be targeted by broadly neutralizing antibodies (bNAbs). Here, we evaluated the ability of a human FP-directed bNAb, VRC34.01, along with two vaccine-elicited anti-FP rhesus macaque mAbs, DFPH-a.15 and DF1W-a.01, to protect against simian-HIV (SHIV)BG505 challenge. VRC34.01 neutralized SHIVBG505 with a 50% inhibitory concentration (IC50) of 0.58 μg/ml, whereas DF1W-a.01 and DFPH-a.15 were 4- or 30-fold less potent, respectively. VRC34.01 was infused into four rhesus macaques at a dose of 10 mg/kg and four rhesus macaques at a dose of 2.5 mg/kg. The animals were intrarectally challenged 5 days later with SHIVBG505. In comparison with all 12 control animals that became infected, all four animals infused with VRC34.01 (10 mg/kg) and three out of four animals infused with VRC34.01 (2.5 mg/kg) remained uninfected. Because of the lower potency of DF1W-a.01 and DFPH-a.15 against SHIVBG505, we infused both Abs at a higher dose of 100 mg/kg into four rhesus macaques each, followed by SHIVBG505 challenge 5 days later. Three of four animals that received DF1W-a.01 were protected against infection, whereas all animals that received DFPH-a.15 were protected. Overall, the protective serum neutralization titers observed in these animals were similar to what has been observed for other bNAbs in similar SHIV infection models and in human clinical trials. In conclusion, FP-directed mAbs can thus provide dose-dependent in vivo protection against mucosal SHIV challenges, supporting the development of prophylactic vaccines targeting the HIV-1 Env FP. Editor's summary: The fusion peptide (FP) on the HIV-1 envelope protein is a conserved site that can be targeted by neutralizing antibodies. However, the extent of protection conferred by FP-directed antibodies in the context of mucosal infection is unclear. Here, Pegu et al. tested three different anti-FP antibodies, including one human antibody and two rhesus macaque antibodies, for their ability to neutralize HIV-1 in vitro and confer protection against simian-HIV (SHIV) challenge of rhesus macaques in vivo. All three antibodies conferred protection against mucosal challenge with SHIV at clinically meaningful titers, supporting further development of both anti-FP antibody therapies and vaccines designed to elicit anti-FP humoral responses. —Courtney Malo [ABSTRACT FROM AUTHOR]

Herein, we describe the efficient gram‐scale synthesis of α2,3‐ and α2,6‐sialyllactose oligosaccharides as well as mimetics from N‐acyl mannosamines and lactose in metabolically engineered bacterial cells grown at high cell density. We designed new Escherichia coli strains co‐expressing sialic acid synthase and N‐acylneuraminate cytidylyltransferase from Campylobacter jejuni together with the α2,3‐sialyltransferase from Neisseria meningitidis or the α2,6‐sialyltransferase from Photobacterium sp. JT‐ISH‐224. Using their mannose transporter, these new strains actively internalized N‐acetylmannosamine (ManNAc) and its N‐propanoyl (N‐Prop), N‐butanoyl (N‐But) and N‐phenylacetyl (N‐PhAc) analogs and converted them into the corresponding sialylated oligosaccharides, with overall yields between 10 % and 39 % (200‐700 mg.L−1 of culture). The three α2,6‐sialyllactose analogs showed similar binding affinity for Sambucus nigra SNA−I lectin as for the natural oligosaccharide. They also proved to be stable competitive inhibitors of Vibrio cholerae neuraminidase. These N‐acyl sialosides therefore hold promise for the development of anti‐adhesion therapy against influenza viral infections. [ABSTRACT FROM AUTHOR]

Higher carbon saccharide 3‐deoxy‐d‐manno‐oct‐2‐ulosonic acid (Kdo) is a structural unit of bacterial lipopolysaccharides (LPSs) and capsular polysaccharides (CPSs). Kdo is present in the inner core region of LPSs, and this region is structurally conserved. Being non‐mammalian in origin, Kdos are effectively recognized by the native and adaptive immune systems. Therefore, the synthesis of new Kdo derivatives and neoglycoconjugates is highly important for the development of vaccines. This review highlights recent accomplishments related to α‐glycosylations, β‐glycosylations and C‐glycosylations of Kdos and their application to the stereoselective synthesis of inner core oligosaccharides. [ABSTRACT FROM AUTHOR]

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Introduction: We adopt the method of bibliometrics to analyze the annual volume of papers and author distribution in the field of campus emergency management in the past 43 years, to evaluate the main population and types of emergency in this study of campus emergency management, and to discuss the existing management methods. Thus, we explore the research hotspot, research gaps and provide reference for future research. Method: We made a statistical analysis of published literature through bibliometrics, searched relevant papers on campus emergency management in PubMed, Embase and Web of Science databases and included 364 valid literature works after screening. The characteristics were analyzed from multiple perspectives. Results: The research on campus emergency management is divided into three stages: "incubation period", "fluctuating growth period" and "rapid development period". Studies vary widely from continent to continent. There are many differences in the focus groups of different school types in the literature. The proportion of emergencies is divided into wound, disaster, school violence, illness and bombshell and others, accounting for 34.17%, 16.94%, 16.11%, 12.78%, 4.44% and 15.56%, respectively. Conclusion: Through the statistical analysis of English literature on campus emergency management from 1977 to 2020, it can be seen that the uneven distribution of research areas is very serious, and there is not much attention to special types of schools. The subdivision and management of the population are in continuous improvement. The research on campus emergency management has been continuously carried out and improved, with a promising prospect. Practical applications: This study can help explain the current situation and development trend of international campus emergency management, and provide reference for further research. [ABSTRACT FROM AUTHOR]

Cell surface sugar 5,7-diacetyl pseudaminic acid (Pse5Ac7Ac) is a bacterial analogue of the ubiquitous sialic acid, Neu5Ac, and contributes to the virulence of a number of multidrug resistant bacteria, including ESKAPE pathogens Pseudomonas aeruginosa, and Acinetobacter baumannii. Despite its discovery in the surface glycans of bacteria over thirty years ago, to date no glycosyltransferase enzymes (GTs) dedicated to the synthesis of a pseudaminic acid glycosidic linkage have been unequivocally characterised in vitro. Herein we demonstrate that A. baumannii KpsS1 is a dedicated pseudaminyltransferase enzyme (PseT) which constructs a Pse5Ac7Ac-α(2,6)-Glcp linkage, and proceeds with retention of anomeric configuration. We utilise this PseT activity in tandem with the biosynthetic enzymes required for CMP-Pse5Ac7Ac assembly, in a two-pot, seven enzyme synthesis of an α-linked Pse5Ac7Ac glycoside. Due to its unique activity and protein sequence, we also assign KpsS1 as the prototypical member of a previously unreported GT family (GT118)., (© 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)

Herein, we describe the efficient gram-scale synthesis of α2,3- and α2,6-sialyllactose oligosaccharides as well as mimetics from N-acyl mannosamines and lactose in metabolically engineered bacterial cells grown at high cell density. We designed new Escherichia coli strains co-expressing sialic acid synthase and N-acylneuraminate cytidylyltransferase from Campylobacter jejuni together with the α2,3-sialyltransferase from Neisseria meningitidis or the α2,6-sialyltransferase from Photobacterium sp. JT-ISH-224. Using their mannose transporter, these new strains actively internalized N-acetylmannosamine (ManNAc) and its N-propanoyl (N-Prop), N-butanoyl (N-But) and N-phenylacetyl (N-PhAc) analogs and converted them into the corresponding sialylated oligosaccharides, with overall yields between 10 % and 39 % (200-700 mg.L -1 of culture). The three α2,6-sialyllactose analogs showed similar binding affinity for Sambucus nigra SNA-I lectin as for the natural oligosaccharide. They also proved to be stable competitive inhibitors of Vibrio cholerae neuraminidase. These N-acyl sialosides therefore hold promise for the development of anti-adhesion therapy against influenza viral infections., (© 2023 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.)