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Your search keyword '"Tweed, Joseph A"' showing total 3 results
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3 results on '"Tweed, Joseph A"'

2. Abstract 1764: Development of an alphalex™-auristatin low pH targeting conjugate for the treatment of solid tumors

Author

Gayle, Sophia, primary, Aiello, Robert, additional, Bechtold, Jane, additional, Bourassa, Patricia, additional, Csengery, Johanna, additional, Hagen, Connor, additional, Howard, Katia, additional, Jones, Kelli, additional, Lopresti-Morrow, Lori, additional, Maguire, Robert, additional, Paradis, Timothy, additional, Pasqualini, Theresa, additional, Tweed, Joseph, additional, Tylaska, Laurie, additional, Zhang, Qing, additional, and Paralkar, Vishwas, additional

Published
2022
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3. Bioanalysis of the Bicycle ® toxin conjugate BT5528 and released monomethyl auristatin E via liquid chromatography-tandem mass spectrometry.

Author

Tweed JA, Adams-Dam F, Allanson J, Holmes K, Senior R, Xu H, Li M, Bennett G, and Jeffrey P

Subjects
Humans, Chromatography, Liquid methods, Tandem Mass Spectrometry methods, Bicycling, Immunotoxins analysis, Immunoconjugates analysis, Antineoplastic Agents, Oligopeptides
Abstract

Background: The Bicycle ® toxin conjugate BT5528 is a novel peptide therapeutic conjugated to the cytotoxic agent monomethyl auristatin E (MMAE). A bioanalytical assay was developed to quantify BT5528 and unconjugated MMAE in human plasma. Methodology: BT5528 quantitation used a protein precipitation procedure followed by LC-MS/MS detection. Quantitation of MMAE required a selective offline and online solid-phase extraction with detection via LC-MS/MS. Results: BT5528 was quantified over the assay range of 5-2500 ng/ml and free MMAE was quantified over the assay range of 0.05-50 ng/ml. Conclusion: Bioanalytical methods were used in the bioanalysis of intact BT5528 and released MMAE, in a phase I/IIa clinical trial; to date, over 2000 human patient samples have been analyzed.

Published
2024
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