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148 results on '"Tsuruyama, Tatsuaki"'

1. Large Deviation Theory Approach to Fluctuation Theorems and Heat Redefinition in Nonequilibrium Reaction Systems

Author

Tsuruyama, Tatsuaki

Subjects
Physics - Chemical Physics
Abstract

In this study, we analyze chemical reaction systems by applying large deviation theory (LDT) and demonstrate that the fluctuation theorem (FT) in nonequilibrium reaction systems can be derived based on the symmetry of the cumulant generating function (CGF) defined through the rate function. Furthermore, we redefine heat through this rate function, establishing a direct connection between heat and Shannon entropy. This redefinition allows for the evaluation of Landauer's principle, which concerns the minimum energy cost of information erasure. The results obtained highlight the effectiveness of LDT as a framework for various nonequilibrium systems.

Published
2024

18. Prostaglandin E2-EP2/EP4 signaling induces immunosuppression in human cancer by impairing bioenergetics and ribosome biogenesis in immune cells.

Author

Punyawatthananukool, Siwakorn, Matsuura, Ryuma, Wongchang, Thamrong, Katsurada, Nao, Tsuruyama, Tatsuaki, Tajima, Masaki, Enomoto, Yutaka, Kitamura, Toshio, Kawashima, Masahiro, Toi, Masakazu, Yamanoi, Koji, Hamanishi, Junzo, Hisamori, Shigeo, Obama, Kazutaka, Charoensawan, Varodom, Thumkeo, Dean, and Narumiya, Shuh

Abstract

While prostaglandin E2 (PGE2) is produced in human tumor microenvironment (TME), its role therein remains poorly understood. Here, we examine this issue by comparative single-cell RNA sequencing of immune cells infiltrating human cancers and syngeneic tumors in female mice. PGE receptors EP4 and EP2 are expressed in lymphocytes and myeloid cells, and their expression is associated with the downregulation of oxidative phosphorylation (OXPHOS) and MYC targets, glycolysis and ribosomal proteins (RPs). Mechanistically, CD8+ T cells express EP4 and EP2 upon TCR activation, and PGE2 blocks IL-2-STAT5 signaling by downregulating Il2ra, which downregulates c-Myc and PGC-1 to decrease OXPHOS, glycolysis, and RPs, impairing migration, expansion, survival, and antitumor activity. Similarly, EP4 and EP2 are induced upon macrophage activation, and PGE2 downregulates c-Myc and OXPHOS in M1-like macrophages. These results suggest that PGE2-EP4/EP2 signaling impairs both adaptive and innate immunity in TME by hampering bioenergetics and ribosome biogenesis of tumor-infiltrating immune cells. Mechanisms of prostaglandin E2 (PGE2)-mediated immunosuppression in the tumor microenvironment (TME) have been previously reported. Here, the authors profile PGE2 functions in human cancer, suggesting that prostaglandin E2-mediated signaling impairs the activity of human CD8+ T cells and macrophages by altering bioenergetics and ribosome biogenesis. [ABSTRACT FROM AUTHOR]

Published
2024
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20. Pathogenicity of IgG-Fc desialylation and its association with Th17 cells in an animal model of systemic lupus erythematosus.

Author

Nishida, Yuri, Shirakashi, Mirei, Hashii, Noritaka, Nakashima, Ran, Nakayama, Yoichi, Katsushima, Masao, Watanabe, Ryu, Onizawa, Hideo, Hiwa, Ryosuke, Tsuji, Hideaki, Kitagori, Koji, Akizuki, Shuji, Onishi, Akira, Murakami, Kosaku, Yoshifuji, Hajime, Tanaka, Masao, Tsuruyama, Tatsuaki, Morinobu, Akio, and Hashimoto, Motomu

Subjects
SYSTEMIC lupus erythematosus, T helper cells, AUTOIMMUNE diseases, IMMUNOGLOBULIN class switching, ANIMAL models in research, DISEASE exacerbation, BETA-glucans
Abstract

Objectives: Decreased sialylation of IgG-Fc glycans has been reported in autoimmune diseases, but its role in systemic lupus erythematosus (SLE) is not fully understood. In this study, we examined the pathogenicity of IgG desialylation and its association with Th17 in SLE using an animal model. Methods: B6SKG mice, which develop lupus-like systemic autoimmunity due to the ZAP70 mutation, were used to investigate the pathogenicity of IgG desialylation. The proportion of sialylated IgG was compared between B6SKG and wild-type mice with or without β-glucan treatment-induced Th17 expansion. Anti-interleukin (IL)-23 and anti-IL-17 antibodies were used to examine the role of Th17 cells in IgG glycosylation. Activation-induced cytidine deaminase–specific St6gal1 conditionally knockout (cKO) mice were generated to examine the direct effect of IgG desialylation. Results: The proportions of sialylated IgG were similar between B6SKG and wild-type mice in the steady state. However, IgG desialylation was observed after β-glucan-induced Th17 expansion, and nephropathy also worsened in B6SKG mice. Anti-IL-23/17 treatment suppressed IgG desialylation and nephropathy. Glomerular atrophy was observed in the cKO mice, suggesting that IgG desialylation is directly involved in disease exacerbation. Conclusions: IgG desialylation contributes to the progression of nephropathy, which is ameliorated by blocking IL-17A or IL-23 in an SLE mouse model. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Anti-complement 5 antibody ameliorates antibody-mediated rejection after liver transplantation in rats

Author

Tajima, Tetsuya, primary, Hata, Koichiro, additional, Kusakabe, Jiro, additional, Miyauchi, Hidetaka, additional, Badshah, Joshua Sam, additional, Kageyama, Shoichi, additional, Zhao, Xiangdong, additional, Kim, Sung-Kwon, additional, Tsuruyama, Tatsuaki, additional, Kirchner, Varvara A., additional, Watanabe, Takeshi, additional, Uemoto, Shinji, additional, and Hatano, Etsuro, additional

Published
2023
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24. Pathogenicity of IgG-Fc desialylation and its association with Th17 cells in an animal model of systemic lupus erythematosus

Author

Nishida, Yuri, primary, Shirakashi, Mirei, additional, Hashii, Noritaka, additional, Nakashima, Ran, additional, Nakayama, Yoichi, additional, Katsushima, Masao, additional, Watanabe, Ryu, additional, Onizawa, Hideo, additional, Hiwa, Ryosuke, additional, Tsuji, Hideaki, additional, Kitagori, Koji, additional, Akizuki, Shuji, additional, Onishi, Akira, additional, Murakami, Kosaku, additional, Yoshifuji, Hajime, additional, Tanaka, Masao, additional, Tsuruyama, Tatsuaki, additional, Morinobu, Akio, additional, and Hashimoto, Motomu, additional

Published
2023
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27. Supplementary Table from Concurrent Activation of Kras and Canonical Wnt Signaling Induces Premalignant Lesions That Progress to Extrahepatic Biliary Cancer in Mice

Author

Nagao, Munemasa, primary, Fukuda, Akihisa, primary, Omatsu, Mayuki, primary, Namikawa, Mio, primary, Sono, Makoto, primary, Fukunaga, Yuichi, primary, Masuda, Tomonori, primary, Araki, Osamu, primary, Yoshikawa, Takaaki, primary, Ogawa, Satoshi, primary, Masuo, Kenji, primary, Goto, Norihiro, primary, Hiramatsu, Yukiko, primary, Muta, Yu, primary, Tsuda, Motoyuki, primary, Maruno, Takahisa, primary, Nakanishi, Yuki, primary, Taketo, Makoto Mark, primary, Ferrer, Jorge, primary, Tsuruyama, Tatsuaki, primary, Nakanuma, Yasuni, primary, Taura, Kojiro, primary, Uemoto, Shinji, primary, and Seno, Hiroshi, primary

Published
2023
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28. Supplementary Data from Concurrent Activation of Kras and Canonical Wnt Signaling Induces Premalignant Lesions That Progress to Extrahepatic Biliary Cancer in Mice

Author

Nagao, Munemasa, primary, Fukuda, Akihisa, primary, Omatsu, Mayuki, primary, Namikawa, Mio, primary, Sono, Makoto, primary, Fukunaga, Yuichi, primary, Masuda, Tomonori, primary, Araki, Osamu, primary, Yoshikawa, Takaaki, primary, Ogawa, Satoshi, primary, Masuo, Kenji, primary, Goto, Norihiro, primary, Hiramatsu, Yukiko, primary, Muta, Yu, primary, Tsuda, Motoyuki, primary, Maruno, Takahisa, primary, Nakanishi, Yuki, primary, Taketo, Makoto Mark, primary, Ferrer, Jorge, primary, Tsuruyama, Tatsuaki, primary, Nakanuma, Yasuni, primary, Taura, Kojiro, primary, Uemoto, Shinji, primary, and Seno, Hiroshi, primary

Published
2023
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29. Supplementary Figure from Concurrent Activation of Kras and Canonical Wnt Signaling Induces Premalignant Lesions That Progress to Extrahepatic Biliary Cancer in Mice

Author

Nagao, Munemasa, primary, Fukuda, Akihisa, primary, Omatsu, Mayuki, primary, Namikawa, Mio, primary, Sono, Makoto, primary, Fukunaga, Yuichi, primary, Masuda, Tomonori, primary, Araki, Osamu, primary, Yoshikawa, Takaaki, primary, Ogawa, Satoshi, primary, Masuo, Kenji, primary, Goto, Norihiro, primary, Hiramatsu, Yukiko, primary, Muta, Yu, primary, Tsuda, Motoyuki, primary, Maruno, Takahisa, primary, Nakanishi, Yuki, primary, Taketo, Makoto Mark, primary, Ferrer, Jorge, primary, Tsuruyama, Tatsuaki, primary, Nakanuma, Yasuni, primary, Taura, Kojiro, primary, Uemoto, Shinji, primary, and Seno, Hiroshi, primary

Published
2023
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30. Data from Concurrent Activation of Kras and Canonical Wnt Signaling Induces Premalignant Lesions That Progress to Extrahepatic Biliary Cancer in Mice

Author

Nagao, Munemasa, primary, Fukuda, Akihisa, primary, Omatsu, Mayuki, primary, Namikawa, Mio, primary, Sono, Makoto, primary, Fukunaga, Yuichi, primary, Masuda, Tomonori, primary, Araki, Osamu, primary, Yoshikawa, Takaaki, primary, Ogawa, Satoshi, primary, Masuo, Kenji, primary, Goto, Norihiro, primary, Hiramatsu, Yukiko, primary, Muta, Yu, primary, Tsuda, Motoyuki, primary, Maruno, Takahisa, primary, Nakanishi, Yuki, primary, Taketo, Makoto Mark, primary, Ferrer, Jorge, primary, Tsuruyama, Tatsuaki, primary, Nakanuma, Yasuni, primary, Taura, Kojiro, primary, Uemoto, Shinji, primary, and Seno, Hiroshi, primary

Published
2023
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35. Increased number of T cells and exacerbated inflammatory pathophysiology in a human IgG4 knock-in MRL/lpr mouse model

Author

70599927, 50626637, 10294216, 20422975, Gon, Yoshie, Kandou, Tsugumitsu, Tsuruyama, Tatsuaki, Iwasaki, Takeshi, Kitagori, Koji, Murakami, Kosaku, Nakashima, Ran, Akizuki, Shuji, Morinobu, Akio, Hikida, Masaki, Mimori, Tsuneyo, Yoshifuji, Hajime, 70599927, 50626637, 10294216, 20422975, Gon, Yoshie, Kandou, Tsugumitsu, Tsuruyama, Tatsuaki, Iwasaki, Takeshi, Kitagori, Koji, Murakami, Kosaku, Nakashima, Ran, Akizuki, Shuji, Morinobu, Akio, Hikida, Masaki, Mimori, Tsuneyo, and Yoshifuji, Hajime

Abstract

Immunoglobulin (Ig) G4 is an IgG subclass that can exhibit inhibitory functions under certain conditions because of its capacity to carry out Fab-arm exchange, inability to form immune complexes, and lack of antibody-dependent and complement-dependent cytotoxicity. Although several diseases have been associated with IgG4, its role in the disease pathogeneses remains unclear. Since mice do not express an IgG subclass that is identical to the human IgG4 (hIgG4), we generated hIGHG4 knock-in (KI) mice and analyzed their phenotypes. To preserve the rearrangement of the variable, diversity, and joining regions in the IGH gene, we transfected a constant region of the hIGHG4 gene into C57BL/6NCrSlc mice by using a gene targeting method. Although the mRNA expression of hIGHG4 was detected in the murine spleen, the serum level of the hIgG4 protein was low in C57BL/6-IgG4KI mice. To enhance the production of IgG4, we established an MRL/lpr-IgG4KI mice model by backcrossing. These mice showed a high IgG4 concentration in the sera and increased populations of IgG4-positive plasma cells and CD3+B220+CD138+ T cells in the spleen. Moreover, these mice showed aggravated inflammation in organs, such as the salivary glands and stomach. The MRL/lpr-IgG4KI mouse model established in the present study might be useful for studying IgG4-related disease, IgG4-type antibody-related diseases, and allergic diseases.

Published
2023

36. Neutrophil S100A9 supports M2 macrophage niche formation in granulomas

Author

50701843, 40706173, 80333532, Mizutani, Tatsuaki, Ano, Toshiaki, Yoshioka, Yuya, Mizuta, Satoshi, Takemoto, Keiko, Ouchi, Yuki, Morita, Daisuke, Kitano, Satsuki, Miyachi, Hitoshi, Tsuruyama, Tatsuaki, Fujiwara, Nagatoshi, Sugita, Masahiko, 50701843, 40706173, 80333532, Mizutani, Tatsuaki, Ano, Toshiaki, Yoshioka, Yuya, Mizuta, Satoshi, Takemoto, Keiko, Ouchi, Yuki, Morita, Daisuke, Kitano, Satsuki, Miyachi, Hitoshi, Tsuruyama, Tatsuaki, Fujiwara, Nagatoshi, and Sugita, Masahiko

Published
2023

37. Best Practices:Recommendations for Repositories

Author

Abdelhafiz, Ahmed Samir, Quesada, Rocio Aguilar, Battiston, Christopher, Bledsoe, Marianna, Castelhano, Marta, Chadwick, Dianne, Chakraborty, Nilay, Edler, Michael, Ellis, Helena, Furlong, Sarah, Gabancho, Nicola, Garcia, Debra Leiolani, Healy, Lyn, Henderson, Marianne, Higgins, Samantha, Holanda, Paulo, Hunt, Charles, Hurtado-Ortiz, Raquel, James, Eric, Kilbride, Peter, Kulkarni, Manisha, Lessard, Carl, Lloyd, Kirsty, Maseme, Mantombi, Michalska-Falkowska, Anna, Michels, Cheryl, Moors, Amanda, Nansumba, Hellen, Nguyen, Trinh, Ovcharenko, Adelina, Paul, Shonali, Jeon, Jae Pil, Pope, Ian, Pugh, Rebecca, Rainbolt, Dean, Ramirez, Nilsa, Schleif, William, Seberg, Ole, Singh, Purnima, Stacey, Glyn, Tsuruyama, Tatsuaki, Upton, Rose, Vollmer, Rainer, Willers, Craig, Woods, Erik, Zaayenga, Andy, Abdelhafiz, Ahmed Samir, Quesada, Rocio Aguilar, Battiston, Christopher, Bledsoe, Marianna, Castelhano, Marta, Chadwick, Dianne, Chakraborty, Nilay, Edler, Michael, Ellis, Helena, Furlong, Sarah, Gabancho, Nicola, Garcia, Debra Leiolani, Healy, Lyn, Henderson, Marianne, Higgins, Samantha, Holanda, Paulo, Hunt, Charles, Hurtado-Ortiz, Raquel, James, Eric, Kilbride, Peter, Kulkarni, Manisha, Lessard, Carl, Lloyd, Kirsty, Maseme, Mantombi, Michalska-Falkowska, Anna, Michels, Cheryl, Moors, Amanda, Nansumba, Hellen, Nguyen, Trinh, Ovcharenko, Adelina, Paul, Shonali, Jeon, Jae Pil, Pope, Ian, Pugh, Rebecca, Rainbolt, Dean, Ramirez, Nilsa, Schleif, William, Seberg, Ole, Singh, Purnima, Stacey, Glyn, Tsuruyama, Tatsuaki, Upton, Rose, Vollmer, Rainer, Willers, Craig, Woods, Erik, and Zaayenga, Andy

Published
2023

38. Increased number of T cells and exacerbated inflammatory pathophysiology in a human IgG4 knock-in MRL/lpr mouse model

Author

Gon, Yoshie, primary, Kandou, Tsugumitsu, additional, Tsuruyama, Tatsuaki, additional, Iwasaki, Takeshi, additional, Kitagori, Koji, additional, Murakami, Kosaku, additional, Nakashima, Ran, additional, Akizuki, Shuji, additional, Morinobu, Akio, additional, Hikida, Masaki, additional, Mimori, Tsuneyo, additional, and Yoshifuji, Hajime, additional

Published
2023
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41. Enteric Toll-like receptor 7 stimulation causes acute exacerbation in lupus-susceptible mice

Author

Takase, Yudai, primary, Shirakashi, Mirei, additional, Nishida, Yuri, additional, Katsushima, Masao, additional, Onizawa, Hideo, additional, Hiwa, Ryosuke, additional, Tsuji, Hideaki, additional, Kitagori, Koji, additional, Akizuki, Shuji, additional, Onishi, Akira, additional, Nakashima, Ran, additional, Murakami, Kosaku, additional, Yoshifuji, Hajime, additional, Tanaka, Masao, additional, Tsuruyama, Tatsuaki, additional, Morinobu, Akio, additional, and Hashimoto, Motomu, additional

Published
2022
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42. Clinically relevant model of oxaliplatin‐induced sinusoidal obstruction syndrome

Author

Toda, Rei, primary, Seo, Satoru, additional, Uemoto, Yusuke, additional, Morino, Koshiro, additional, Nishino, Hiroto, additional, Nakamura, Naohiko, additional, Okuno, Masayuki, additional, Iguchi, Kohta, additional, Sato, Motohiko, additional, Nakamura, Kojiro, additional, Taura, Kojiro, additional, Nakagawa, Shunsaku, additional, Nakagawa, Takayuki, additional, Tsuruyama, Tatsuaki, additional, Manabe, Toshiaki, additional, Kawaguchi, Hiroaki, additional, Iwaisako, Keiko, additional, Ikegawa, Masaya, additional, Uemoto, Shinji, additional, and Hatano, Etsuro, additional

Published
2022
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43. Bile Duct Regeneration with an Artificial Bile Duct Made of Gelatin Hydrogel Nonwoven Fabrics

Author

Uemoto, Yusuke, primary, Taura, Kojiro, additional, Nakamura, Daichi, additional, Xuefeng, Li, additional, Nam, Nguyen Hai, additional, Kimura, Yusuke, additional, Yoshino, Kenji, additional, Fuji, Hiroaki, additional, Yoh, Tomoaki, additional, Nishio, Takahiro, additional, Yamamoto, Gen, additional, Koyama, Yukinori, additional, Seo, Satoru, additional, Tsuruyama, Tatsuaki, additional, Iwaisako, Keiko, additional, Uemoto, Shinji, additional, Tabata, Yasuhiko, additional, and Hatano, Etsuro, additional

Published
2022
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44. Simultaneous activation of Kras–Akt and Notch pathways induces extrahepatic biliary cancer via the mTORC1 pathway.

Author

Namikawa, Mio, Fukuda, Akihisa, Mizukoshi, Kenta, Iwane, Kosuke, Kawai, Munenori, Yamakawa, Go, Omatsu, Mayuki, Sono, Makoto, Masuda, Tomonori, Araki, Osamu, Nagao, Munemasa, Yoshikawa, Takaaki, Ogawa, Satoshi, Hiramatsu, Yukiko, Muta, Yu, Tsuda, Motoyuki, Maruno, Takahisa, Nakanishi, Yuki, Tsuruyama, Tatsuaki, and Taura, Kojiro

Subjects
BILIARY tract cancer, PRECANCEROUS conditions, RIBOSOMAL proteins, BILE ducts, CARCINOGENESIS
Abstract

Biliary tract cancer (BTC) has poor prognosis. The Notch receptor is aberrantly expressed in extrahepatic cholangiocarcinoma (eCCA). However, the role of Notch signaling in the initiation and progression of eCCA and gallbladder (GB) cancer remains unknown. Therefore, we investigated the functional role of Notch signaling during tumorigenesis of the extrahepatic bile duct (EHBD) and GB. Activation of Notch signaling and oncogenic Kras resulted in the development of biliary intraepithelial neoplasia (BilINs) in the EHBD and GB, which were premalignant lesions that progressed to adenocarcinoma in mice. The expression of genes involved in the mTORC1 pathway was increased in biliary spheroids from Hnf1b‐CreERT2; KrasLSL‐G12D; Rosa26LSL‐NotchIC mice and inhibition of the mTORC1 pathway suppressed spheroid growth. Additionally, simultaneous activation of the PI3K–AKT and Notch pathways in EHBD and GB induced biliary cancer development in mice. Consistent with this, we observed a significant correlation between activated NOTCH1 and phosphorylated Ribosomal Protein S6 (p‐S6) expression in human eCCA. Furthermore, inhibition of the mTORC1 pathway suppressed the growth of Notch‐activated human biliary cancer cells in vitro and in vivo. Mechanistically, the Kras/Notch–Myc axis activated mTORC1 through TSC2 phosphorylation in mutant biliary spheroids. These data indicate that inhibition of the mTORC1 pathway could be an effective treatment strategy for Notch‐activated human eCCA. © 2023 The Pathological Society of Great Britain and Ireland. [ABSTRACT FROM AUTHOR]

Published
2023
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45. Loss of Arid1a and Pten in Pancreatic Ductal Cells Induces Intraductal Tubulopapillary Neoplasm via the YAP/TAZ Pathway

Author

Fukunaga, Yuichi, primary, Fukuda, Akihisa, additional, Omatsu, Mayuki, additional, Namikawa, Mio, additional, Sono, Makoto, additional, Masuda, Tomonori, additional, Araki, Osamu, additional, Nagao, Munemasa, additional, Yoshikawa, Takaaki, additional, Ogawa, Satoshi, additional, Hiramatsu, Yukiko, additional, Muta, Yu, additional, Tsuda, Motoyuki, additional, Maruno, Takahisa, additional, Nakanishi, Yuki, additional, Ferrer, Jorge, additional, Tsuruyama, Tatsuaki, additional, Masui, Toshihiko, additional, Hatano, Etsuro, additional, and Seno, Hiroshi, additional

Published
2022
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47. The N⁶-methyladenosine methyltransferase METTL16 enables erythropoiesis through safeguarding genome integrity

Author

70878347, 60570140, 10379092, Yoshinaga, Masanori, Han, Kyuho, Morgens, David W., Horii, Takuro, Kobayashi, Ryosuke, Tsuruyama, Tatsuaki, Hia, Fabian, Yasukura, Shota, Kajiya, Asako, Cai, Ting, Cruz, Pedro H. C., Vandenbon, Alexis, Suzuki, Yutaka, Kawahara, Yukio, Hatada, Izuho, Bassik, Michael C., Takeuchi, Osamu, 70878347, 60570140, 10379092, Yoshinaga, Masanori, Han, Kyuho, Morgens, David W., Horii, Takuro, Kobayashi, Ryosuke, Tsuruyama, Tatsuaki, Hia, Fabian, Yasukura, Shota, Kajiya, Asako, Cai, Ting, Cruz, Pedro H. C., Vandenbon, Alexis, Suzuki, Yutaka, Kawahara, Yukio, Hatada, Izuho, Bassik, Michael C., and Takeuchi, Osamu

Abstract

During erythroid differentiation, the maintenance of genome integrity is key for the success of multiple rounds of cell division. However, molecular mechanisms coordinating the expression of DNA repair machinery in erythroid progenitors are poorly understood. Here, we discover that an RNA N⁶-methyladenosine (m⁶A) methyltransferase, METTL16, plays an essential role in proper erythropoiesis by safeguarding genome integrity via the control of DNA-repair-related genes. METTL16-deficient erythroblasts exhibit defective differentiation capacity, DNA damage and activation of the apoptotic program. Mechanistically, METTL16 controls m⁶A deposition at the structured motifs in DNA-repair-related transcripts including Brca2 and Fancm mRNAs, thereby upregulating their expression. Furthermore, a pairwise CRISPRi screen revealed that the MTR4-nuclear RNA exosome complex is involved in the regulation of METTL16 substrate mRNAs in erythroblasts. Collectively, our study uncovers that METTL16 and the MTR4-nuclear RNA exosome act as essential regulatory machinery to maintain genome integrity and erythropoiesis.

Published
2022

48. Bile Duct Regeneration with an Artificial Bile Duct Made of Gelatin Hydrogel Nonwoven Fabrics

Author

20842826, 90450238, 50211371, Uemoto, Yusuke, Taura, Kojiro, Nakamura, Daichi, Xuefeng, Li, Nam, Nguyen Hai, Kimura, Yusuke, Yoshino, Kenji, Fuji, Hiroaki, Yoh, Tomoaki, Nishio, Takahiro, Yamamoto, Gen, Koyama, Yukinori, Seo, Satoru, Tsuruyama, Tatsuaki, Iwaisako, Keiko, Uemoto, Shinji, Tabata, Yasuhiko, Hatano, Etsuro, 20842826, 90450238, 50211371, Uemoto, Yusuke, Taura, Kojiro, Nakamura, Daichi, Xuefeng, Li, Nam, Nguyen Hai, Kimura, Yusuke, Yoshino, Kenji, Fuji, Hiroaki, Yoh, Tomoaki, Nishio, Takahiro, Yamamoto, Gen, Koyama, Yukinori, Seo, Satoru, Tsuruyama, Tatsuaki, Iwaisako, Keiko, Uemoto, Shinji, Tabata, Yasuhiko, and Hatano, Etsuro

Abstract

Although choledochojejunostomy is the standard technique for biliary reconstruction, there are various associated problems that need to be solved such as reflux cholangitis. Interposition with an artificial bile duct (ABD) to replace the resected bile duct maintains a physiological conduit for bile and may solve this problem. This study investigated the usefulness of an ABD made of gelatin hydrogel nonwoven fabric (GHNF). GHNF was prepared by the solution blow spinning method. The migration and activity of murine fibroblast L929 cells were examined in GHNF sheets. L929 cells migrated into GHNF sheets, where they proliferated and synthesized collagen, suggesting GHNF is a promising scaffold for bile duct regeneration. ABDs made of GHNF were implanted in place of resected bile duct segments in rats. The rats were killed at 2, 6, and 12 weeks postimplantation. The implantation site was histologically evaluated for bile duct regeneration. At postoperative 2 weeks, migrating cells were observed in the ABD pores. The implanted ABD was mostly degraded and replaced by collagen fibers at 6 weeks. Ki67-positive bile duct epithelial cells appeared within the implanted ABD. These were most abundant within the central part of the ABD after 6 weeks. The percentages of Ki67-positive cells were 31.7 ± 9.1% in the experimental group and 0.8 ± 0.6% in the sham operation group at 6 weeks (p < 0.05), indicating that mature biliary epithelial cells at the stump proliferated to regenerate the biliary epithelium. Biliary epithelial cells had almost completely covered the bile duct lumen at 12 weeks (epithelialization ratios: 10.4 ± 6.9% at 2 weeks, 93.1 ± 5.1% at 6 weeks, 99.2 ± 1.6% at 12 weeks). The regenerated epithelium was positive for the bile duct epithelium marker cytokeratin 19. Bile duct regeneration was accompanied by angiogenesis, as evidenced by the appearance of CD31-positive vascular structures. Capillaries were induced 2 weeks after implantation. The number of capillaries

Published
2022

49. Concurrent Activation of Kras and Canonical Wnt Signaling Induces Premalignant Lesions That Progress to Extrahepatic Biliary Cancer in Mice

Author

Nagao, Munemasa, primary, Fukuda, Akihisa, additional, Omatsu, Mayuki, additional, Namikawa, Mio, additional, Sono, Makoto, additional, Fukunaga, Yuichi, additional, Masuda, Tomonori, additional, Araki, Osamu, additional, Yoshikawa, Takaaki, additional, Ogawa, Satoshi, additional, Masuo, Kenji, additional, Goto, Norihiro, additional, Hiramatsu, Yukiko, additional, Muta, Yu, additional, Tsuda, Motoyuki, additional, Maruno, Takahisa, additional, Nakanishi, Yuki, additional, Taketo, Makoto Mark, additional, Ferrer, Jorge, additional, Tsuruyama, Tatsuaki, additional, Nakanuma, Yasuni, additional, Taura, Kojiro, additional, Uemoto, Shinji, additional, and Seno, Hiroshi, additional

Published
2022
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50. Novel Techniques in Histologic Research : Morphometry and Mass Spectrometry Imaging

Author

Tsuruyama, Tatsuaki and Hieatsuka, Takuya

Subjects
Medical
Abstract

Application of mass spectrometry (MS) has recently been developed for the identification of biomarkers using pathologic samples, whereas its application using formaldehyde-fixed paraffin-embedded (FFPE) tissues remains limited. In this review, we introduce MS imaging (MSI) using FFPE tissue. This method is still challenging for peptide ionization, and various pretreatment techniques have been conducted for enhancing the ionization signal of peptides. A simple chemical pretreatment method involving heating in acetonitrile-containing buffer under pressurized conditions is introduced. Further, two-dimensional MSI data are summarized in a DM for region of interest (ROI) and hierarchical cluster analyses. These techniques enable MALDI-MSI analysis of archived pathological FFPE samples to identify new biomarkers.

Published
2022

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