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Byline: Liya. He, Nianyi. Zhang, Cuicui. Tian, Shengmei. Zhang, Liangchun. Wang Sir, As an uncommon adverse cutaneous event mimicking lichen planus in morphology and pathology.[1] lichenoid drug eruption (LDE) usually [...]

Pyropia yezoensis is the most widely cultivated and economically important alga. Affected by climate change, the cultivation of P. yezoensis has gradually migrated to the northern coast of China, increasing from 6.8% in 2019 to 19.5% in 2023. To date, the genetic impact of northern migration on cultivation resources has not been assessed and analyzed extensively. Here, DNA barcoding (rbcL and cox1) and the presence/absence of intronic-ORFs in mitochondrial regions (rnl and cox1) were applied to investigate genetic diversity in 44 P. yezoensis specimens from 17 aquaculture farms in China, with comparisons to Korean and Japanese cultivated resources. The lower intraspecific variation was 0.31% for the cox1 gene and 0.14% for the rbcL gene, with three haplotypes, indicating that intensive selection and breeding during cultivation had narrowed the germplasm genetic variation. The intron structure of mitochondrial regions showed that the cultivated resources had 17 phenotypes, and the northern specimens shared 35.3% of genotypes with the southern specimens, indicating that the cultivated P. yezoensis is expanding its cultivation ranges through north migration. Even with lower genetic diversity, the northern area of cultivation had already developed 17.6% site-specific specimens. The genetic diversity of cultivated P. yezoensis from the Northwest Pacific is also discussed. Our work provides a preliminary framework for P. yezoensis breeding and cultivation under climate change. [ABSTRACT FROM AUTHOR]

(E) Skin biopsy showed epidermal hyperplasia with parakeratosis and dense intraepidermal infiltrate of atypical, medium-to-large size lymphocytes Inset images showed pagetoid cells. In summary, PR can be challenging to diagnose and RCM may be a helpful adjunct tool for differential diagnosis, but descriptions of RCM from more cases are needed to confirm this hypothesis. Pagetoid reticulosis (PR) is a rare variant of mycosis fungoides (MF). [Extracted from the article]

Background The recurrence rate of extramammary Paget disease after surgical resection is high due to the lesions' poorly delineated and unclear margins. Aims To evaluate the impact of non-invasive tumour margin detection via photodynamic diagnosis plus reflectance confocal microscopy on the surgical outcomes of patients with extramammary Paget disease. Methods Thirty-six patients with histopathologically confirmed primary extramammary Paget disease between January 2017 and June 2018 were included in this study. The skin lesion margins were preoperatively observed using the naked eye, photodynamic diagnosis, and reflectance confocal microscopy. An incision was made 0.5-2 cm from the outermost non-invasive detection marker line. The incision depth was more significant than the follicle level or the deepest level affected by the tumour in the biopsy specimens. After the skin lesions were removed, a pathological examination of the specimens was conducted to ensure clear margins to prevent tumour recurrence and metastases. Results A total of 166 good-quality tissue sections were selected from 36 patients. The tumour surfaces and deep margins were within the scope of resection. Six patients (6/36, 15.4%) experienced local recurrence 2-12 months postoperatively. One patient (1/36, 2.8%) had lymph node metastasis without local recurrence 36 months postoperatively and died 50 months postoperatively (1/36, 2.8%). Limitations This study is limited by the small patient population, especially the number of patients with mucous membrane involvement. Conclusion Using photodynamic diagnosis plus reflectance confocal microscopy to detect the margins of extramammary Paget disease lesions non-invasively reduces the postoperative tumour recurrence rate and is a valuable guide for tumour treatment.