Search

Abstract This study aimed to evaluate alternative in vivo treatment trials using natural products for ectoparasitic infestation on Nile tilapia; these two products were not previously used in the treatment of parasitic fish diseases. So, a total of 400 Oreochromis niloticus (O. niloticus) fish measured 10–15 cm in length; 350 from a fish farm in (Kafr Elsheikh and 50 from Nile River (Al Bahr Al Aazam), Egypt. The examined fishes were 10–15 ± 0.5 cm long and weighed from 45 g ± 5. The collected fish were examined for different clinical abnormalities. Each part of the fish underwent a careful microscopic examination of mucous surrounding the skin; gills and fins. Two feed supplements were used experimentally to decrease mortality and treat fish against ectoparasites (Herb-All PARA-X® and Herb-All CALM®). Total mRNA was extracted from the gills of different examined groups. Glucose; nitric oxide; cortisol as well as lysozyme activity were assessed in all groups. The gills of the examined fish were collected for histopathological examination. Only, Dactyolgyrus sp. was recovered. The intensity of the parasite was counted per microscopic field. The treated groups showed low levels of the parameters compared to the control positive group. Up-regulation of both Tumor necrosis factor-α (TNF- α) and heat shock protein 70 (hsp-70) were detected in fins, gills, and skin in the infested tilapia. The treatment and prophylaxis significantly downregulated both genes in the studied organ in a dose-dependent manner. Recorded lesions which were scored according to their severity. In conclusion; following the use of those products, fish health has been greatly improved and that is indicated by findings of immune reactions as well as histopathology.

Abstract The global focus has recently shifted away from fuel-based power sources, and one of the most important projects for energy production is wind energy. To maintain low costs, the current research examines the problem of vibrations affecting wind turbine towers’ performance (WTTs). In particular, the tower, resulting from excessive vibrations, can negatively affect a structure’s power output and service life, as it can cause fatigue. Therefore, we conducted numerical tests on various types of controlled systems. Our tests revealed that combining a new technique cubic negative velocity control (CNVC) and linear negative acceleration control (LNAC) was the most effective and cost-efficient option for vibration damping. This solution was derived by using an approximation method for the averaging technique. The external force is an important component of a nonlinear dynamic system and can be characterized by two-degree-of-freedom (2-DOF) differential coupled equations. After implementing the control measures, we conducted a numerical analysis of the vibration values before and after the operation. Stability is studied numerically. The numerical and approximate solutions were confirmed through the frequency response equation and time history with fourth-order Runge–Kutta (RK-4). Finally, we investigated the effect of parameters and compared our results with those of previously published studies.

Abstract To arrive at an equivalent linear differential equation, the non-perturbative approach (NPA) is established. The corresponding linear equation is employed for performing the structural analysis. A numerical computation demonstrates a high consistency with the precise frequency. The correlation with the numerical solution explains the reasonableness of the obtained solutions. For additional nonlinear kinds of oscillation, the methodology gives an exact simulation. The stable construction of the prototype is shown in a series of diagrams. Positive position feedback (PPF), integral resonant control (IRC), nonlinear integral positive position feedback (NIPPF), and negative derivative feedback (NDF) are proposed to get rid of the damaging vibration in the system. It is found that the NDF control is more efficient than other controllers for vibration suppression. The theoretical methodology is applied by using the averaging method for getting a perturbed solution. The stability and influence of various parameters of the structure are established at main and 1:1 internal resonance, which is presented as one of the worst resonance cases. Association concerning mathematical solution and computational simulation is achieved.

Abstract A series of new coumarin-N-heterocyclic hybrids, coumarin-quinolines 7a–e, coumarin-acridines 10b,c and coumarin-neocryptolepines 13b,c were synthesized and evaluated for their anticancer and antimicrobial activities. The structures of all synthesized hybrids were confirmed by FT-IR, 1H-NMR, 13C-NMR, and MS spectrometry. The anti-proliferative activity of hybrids 7a–e, 10c and 13c were bio-evaluated using MTT-assay against colon (CaCo-2), lung (A549), breast (MDA-MB-231), and hepatocellular carcinoma (HepG-2) human cancer cell lines using doxorubicin as a reference drug. The results demonstrated that, all hybrids displayed moderate to good anti-proliferative activity against the cell lines. The most active hybrids were 7a–d and 10c against CaCo-2 cancer cell line with IC50: 57.1, 52.78, 57.29, 51.95 and 56.74 µM, and selectivity index 1.38, 1.76, 2.6, 1.96 and 0.77; respectively. While, 7a,d were potent against A549 cancer cell line with IC50: 51.72, 54.8 µM and selectivity index 1.5, 0.67; respectively. Moreover, 7c showed the most potency against MDA-MB-231 cancer cell line with IC50: 50.96 µM and selectivity index 2.20. Interestingly, docking results revealed that binding energy of the current compounds showed marked affinity values ranging from -6.54 to -5.56 kcal with interactions with the reported key amino acid SER 79. Furthermore, the antimicrobial activity of the synthesized hybrids 7a–e, 10b,c, 13b and 13c were evaluated against Gram‐positive and Gram‐negative bacterial and fungal strains. The hybrids 10b, 13b, 10c, and 13c exhibited broad-spectrum antibacterial activity against E.coli, S. mutans, and S. aureus with MIC from 3.2 to 66 µM, this hybrids also displayed antifungal activity against C. albicans with MIC values ranging from 0.0011 to 29.5 µM. In-silico investigation of the pharmacokinetic properties indicated that tested hybrids had high GI absorption, low Blood Brain Barrier (BBB) permeability in addition to cell membrane penetrability.

This article contemplates the demeanor of the giant magnetostrictive actuator (GMA) when a positive position feedback (PPF) damper is used to enable tight control over its vibration. The methodology followed here mathematically searches for the approximate solution for the motion equations of the GMA with the PPF damper, which has been accomplished by using one of the most famous perturbation methods. The multiple scale perturbation technique (MSPT) of the second-order approximation is our strategy to obtain the analytical results. The stability of the system has also been investigated and observed by implementing frequency response equations to close the concurrent primary and internal resonance cases. By utilizing Matlab and Maple programs, all numerical discussions have been accomplished and explained. The resulting influence on the amplitude due to changes in the parameters' values has been studied by the frequency response curves. Finally, a comparison between both the analytical and numerical solutions using time history and response curves is made. In addition to the comparison between our PPF damper's effect on the GMA, previous works are presented. To get our target in this article, we have mentioned some important applications utilized in the GMA system just to imagine the importance of controlling the GMA vibration.

Abstract The inverted pendulum is controlled in this article by using the nonlinear control theory. From classical analytical mechanics, its substructure equation of motion is derived. Because of the inclusion of the restoring forces, the Taylor expansion is employed to facilitate the analysis. An estimated satisfactory periodic solution is obtained with the aid of the modified Homotopy perturbation method. A numerical technique based on the fourth-order Runge–Kutta method is employed to justify the previous solution. On the other hand, a positive position feedback control is developed to dampen the vibrations of an IP system subjected to multi-excitation forces. The multiple time scale perturbation technique of the second order is introduced as a mathematical method to solve a two-degree-of-freedom system that simulates the IP with the PPF at primary and 1:1 internal resonance. The stability of these solutions is checked with the aid of the Routh–Hurwitz criterion. A set of graphs, based on the frequency response equations resulting from the MSPT method, is incorporated. Additionally, a numerical simulation is set up with RK-4 to confirm the overall controlled performance of the studied model. The quality of the solution is confirmed by the match between the approximate solution and the numerical simulation. Numerous other nonlinear systems can be controlled using the provided control method. Illustrations are offered that pertain to implications in design and pedagogy. The linearized stability of IP near the fixed points as well as the phase portraits is depicted for the autonomous and non-autonomous cases. Because of the static stability of the IP, it is found that its instability can be suppressed by the increase of both the generalized force as well as the torsional constant stiffness of the spring. Additionally, the presence of the magnetic field enhances the stability of IP.

Abstract In the sight of the ever-increasing significance of green-based iron nanoparticles especially in wastewater treatment applications is a compelling reason for their use in a waste prevention opportunity, safer environment and benign precursor materials become the vital considerations. Hence, in the current investigation, an efficient co-precipitation technique was applied to prepare highly active chitosan-coated magnetic iron oxide that is applied for wastewater remediation. In the current investigation, chitosan coupled with magnetite nanoparticles namely CS-M was attained by coupling chitosan (CS) with magnetite nanoparticles via simple co-precipitation in different weight proportions and the attained samples labeled as CS-M-(2:1), CS-M-(3:1) and CS-M-(1:2). The structure, morphology and characteristics of the prepared samples were characterized using X-ray diffraction spectroscopy and transmission electron microscopy (TEM). The catalytic oxidation activity of the prepared samples was investigated to eliminate Basic Blue 9 (BB9) dye from aqueous effluent as simulated textile polluted stream. The experimental data exposed almost BB9 dye emanation. The system parameters revealed the maximal BB9 oxidation (99%) was attained within 2 h of irradiance time. Box–Behnken design factorial design based on response surface methodology was applied to optimize the Fenton’s system (CS-M-(2:1)/H2O2) parameters to maximize the efficiency 2.4 and 767 mg/L of CS-M and H2O2, respectively, at pH 7.0. The experimental data exposed that CS-M-(2:1) is signified as the optimal catalyst mixture. The kinetic data verify the oxidation system follows the second-order reaction kinetic model. Further, thermodynamic variables predicted that the reaction is endothermic and non-spontaneous in nature. Hence, the catalyst could be environmental benign and the evaluation introduces the role of engineers and chemists in a world for a sustainable material use.

Abstract Background Early detection of hepatocellular carcinoma (HCC) is crucial for improving the survival rate for patients. Talin-1 is first identified as a cytoskeleton protein that is required for cell adhesion and motility and plays a role in tumor migration and metastasis. In the present work, we aimed to study the possible role of Talin1 compared to alpha fetoprotein (AFP) in the diagnosis and prognosis of HCC. Methods To achieve this goal, serum levels of Talin-1 were measured using enzyme-linked immunosorbent assay (ELISA) in 90 patients divided into four groups. Group I: 30 patients with early HCC. Group II: 30 patients with late HCC according to Modified Barcelona-Clinic Liver Cancer (BCLC). Group III: 15 patients with liver cirrhosis, and group IV: 15 healthy controls. Receiver operating characteristics (ROC) curve analysis was used to create a predictive model for Talin-1 relative to AFP in HCC diagnosis. Results It was found that serum Talin-1 in HCC patients was significantly higher compared to its level in cirrhotic patients and the healthy control group. Talin-1 was superior to AFP regarding sensitivity, specificity, positive, and negative predictive value in the diagnosis of HCC. We also found a significant positive correlation between serum Talin-1 and the degree of tumor burden of HCC (BCLC staging), tumor size, and vascular invasion. Conclusion Talin-1 holds a promise as a potential marker for HCC diagnosis and prognosis.

Objectives: During the COVID-19 pandemic, a quick and reliable phone-triage system is critical for early care and efficient distribution of hospital resources. The study aimed to assess the accuracy of the traditional phone-triage system and phone triage-driven deep learning model in the prediction of positive COVID-19 patients. Setting: This is a retrospective study conducted at the family medicine department, Cairo University. Methods: The study included a dataset of 943 suspected COVID-19 patients from the phone triage during the first wave of the pandemic. The accuracy of the phone triaging system was assessed. PCR-dependent and phone triage-driven deep learning model for automated classifications of natural human responses was conducted. Results: Based on the RT-PCR results, we found that myalgia, fever, and contact with a case with respiratory symptoms had the highest sensitivity among the symptoms/ risk factors that were asked during the phone calls (86.3%, 77.5%, and 75.1%, respectively). While immunodeficiency, smoking, and loss of smell or taste had the highest specificity (96.9%, 83.6%, and 74.0%, respectively). The positive predictive value (PPV) of phone triage was 48.4%. The classification accuracy achieved by the deep learning model was 66%, while the PPV was 70.5%. Conclusion: Phone triage and deep learning models are feasible and convenient tools for screening COVID-19 patients. Using the deep learning models for symptoms screening will help to provide the proper medical care as early as possible for those at a higher risk of developing severe illness paving the way for a more efficient allocation of the scanty health resources.

Objectives Early detection of coronavirus disease 2019 (COVID-19) is crucial for patients and public health to ensure pandemic control. We aimed to correlate clinical and laboratory data of patients with COVID-19 and their polymerase chain reaction (PCR) results and to assess the accuracy of a deep learning model in diagnosing COVID-19. Methods This was a retrospective study using an anonymized dataset of patients with suspected COVID-19. Only patients with a complete dataset were included (n = 440). A deep analytics framework and dual-modal approach for PCR-based classification was used, integrating symptoms and laboratory-based modalities. Results Participants with loss of smell or taste were two times more likely to have positive PCR results (odds ratio [OR] 1.86). Participants with neutropenia, high serum ferritin, or monocytosis were three, four, and five times more likely to have positive PCR results (OR 2.69, 4.18, 5.42, respectively). The rate of accuracy achieved using the deep learning framework was 78%, with sensitivity of 83.9% and specificity of 71.4%. Conclusion Loss of smell or taste, neutropenia, monocytosis, and high serum ferritin should be routinely assessed with suspected COVID-19 infection. The use of deep learning for diagnosis is a promising tool that can be implemented in the primary care setting.

Introduction: The results of patients at one hospital who were judged eligible for conservative care of abdominal aortic aneurysms (AAA) are examined in this research. Optimizing patient care and management tactics requires an understanding of the mortality trends and causes of death within this group., Methodology: Sunderland Royal Hospital carried out a single-center retrospective analysis between May 2018 and January 2024. Out of the 447 individuals who had a AAA diagnosis, 396 had repairs, while 51 were rejected. Medical records were used to gather information on demographics, aneurysm features, length of survival, and reasons for death. The reasons for death and survival rates were ascertained using statistical analysis., Results: Thirty (58.8%) of the 51 patients had passed away, while 21 (41.1%) were still alive. The remaining 26 fatalities (87%) were ascribed to various causes, such as myocardial infarction (27%), cancer (17%), and chronic obstructive pulmonary disease (COPD) (13%), with just four deaths (13%) being caused by ruptured AAA (rAAA). According to the research, the one-year survival rate was 17.6%, and the first six months after choosing not to intervene were when the majority of deaths occurred., Conclusion: Comorbidities have a substantial influence on outcomes for patients treated conservatively for AAA, as evidenced by the low proportion of rupture-related fatalities. This study emphasizes the need for more investigation, especially large-scale, population-based studies, to gain a better understanding of AAA patient care and outcomes., Competing Interests: Human subjects: Consent for treatment and open access publication was obtained or waived by all participants in this study. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Zulfiquar et al.)

Background: The aging population is a growing challenge for healthcare services and as such multimorbidity and associated aging are the focus of research programs. Chronic limb-threatening ischemia (CLTI) in the older patient is perceived to be associated with high morbidity and mortality but a potentially contentious area with limited evidence., Methods: Retrospective review of all consecutive CLTI admissions to a UK tertiary vascular during 2020. Analysis included descriptive statistics and comparisons by age. The primary outcome was survival (by Kaplan-Meier) with secondary outcomes being major adverse limb and cardiovascular events., Results: One hundred eighty-three patients with a median age of 72 of which 55 (30%) were octogenarians. Fewer octogenarians were diabetic (38.2% vs. 58.6%, P = 0.015), but comorbidities such as previous stroke (25.5% vs. 10.9%, P = 0.015) and atrial fibrillation (36.4% vs. 16.4%, P = 0.004) were increasingly common. 87.3% of octogenarians had moderate or severe frailty compared to 57.8% in those <80 (P = 0.001) (by electronic frailty index). Median survival time was 30 months with no significant difference between groups (P = 0.406). Major adverse cardiovascular event (10.9% vs. 7.81%, P = 0.504) and major adverse limb event (34.5% vs. 24.2% P = 0.261) were comparable between groups. Octogenarians were less likely to have open surgery (10.9% vs. 25.8%, P = 0.024)., Conclusions: Octogenarians have similar cardiovascular, limb, and survival outcomes following intervention despite being increasingly frail and comorbid. Holistic assessment, perioperative optimization, and risk stratification are important in this group., (Crown Copyright © 2024. Published by Elsevier Inc. All rights reserved.)

Newly synthesized vaccines prepared from formalin-killed bacteria Streptococcus pyogenes were investigated in the current study to evaluate the effectiveness of the newly synthesized vaccine as well as their safety by injected intraperitoneal. The study involved several steps 1st step is the preparation of the vaccine followed by the 2nd step: Evaluate the effectiveness and vaccine safety against pathogenic S. pyogenes through 4 different groups including control (Group I). Group II (Bacterial, infected group), Group III (Vaccine), and the Last group was the challenged group after the vaccination (Vacc + Bac). Different Immunological and biochemical parameters were measured in addition to hematological and histopathological examinations. For example, oxidative/antioxidants, inflammatory biomarkers, fragmentation and cell damage, and finally the histopathological study. The current study showed an increase in all oxidative, inflammatory, and cell damage (DNA fragmentation assays), additionally markedly elevation in histopathological cell damage in the infected group (Group II) compared with the control group. The vaccine and challenged after vaccination group (vaccine + Bacteria), showed great improvement in oxidative biomarkers (LPO) and an increase in antioxidants biomarkers (GSH, SOD, GST, DPPH, ABTS, GR and GPx), Also the inflammation and histopathological examination. The newly synthesized vaccine improved the resistance of Oreochromis niloticus and can be used as a preventive therapy agent for pathogenic bacteria S. pyogenes., (© 2024. The Author(s).)

A new decadentate chelator, H 2 ampa, was designed to be a potential radiopharmaceutical chelator component. The chelator involves both amide and picolinate functional groups on a large non-macrocyclic, ether-bridged backbone. With its large scaffold, H 2 ampa was paired with [ nat/203 Pb]Pb 2+ , [ nat/213 Bi]Bi 3+ , and nat La 3+ /[ 225 Ac]Ac 3+ ions. Nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry were used to study the non-radioactive metal complexes. A single crystal of [Bi(ampa)](NO 3 ) was obtained; its asymmetric, 10-coordinate complex structure was revealed by X-ray diffraction. Optimal conformations of the metal complexes were assessed by density functional theory studies to provide further structural information. Solution studies providing thermodynamic insights into metal complex formation revealed H 2 ampa coordinated Bi 3+ , Pb 2+ , and La 3+ ions to obtain pM values of 26, 14.8, and 15.1, respectively. Preliminary concentration-dependent radiolabeling experiments were carried out between H 2 ampa and three different radiometals to evaluate their compatibility for radiopharmaceutical applications. The chelator radiolabeled [ 203 Pb]Pb 2+ , [ 213 Bi]Bi 3+ , and [ 225 Ac]Ac 3+ in short reaction times (7-30 min), at dilute concentrations, and under mild conditions. Thus, H 2 ampa was proven to be a versatile chelator able to well coordinate a small range of radiometals frequently considered to be alpha therapeutic candidates.

Silver nanoparticles (AgNPs) are a potentially effective tool for preventing viral plant diseases. This study was carried out to evaluate the effectiveness of AgNPs for managing bean yellow mosaic virus (BYMV) disease in faba bean plants from the plant-virus-vector interaction side. AgNPs were evaluated as foliar protective and curative agents. In addition, the effect of AgNPs on virus acquisition and transmission by its vector aphid was investigated. The results indicated that AgNPs exhibited curative viricidal activity and were able to inactivate BYMV when applied 48 hours after virus inoculation. The occurrence of disease was prevented using an AgNP concentration as low as 100 mg L -1 , whereas virus infection was completely inhibited when plants were preventatively treated with AgNPs at a concentration of to 200 mg L -1 24 h before virus inoculation. AgNPs proved to be highly bio-reactive, binding to viral particles and suppressing their replication and accumulation within plant tissues. Moreover, AgNPs, at all concentrations tested, were found to upregulate the pathogenesis-related gene PR-1 and induce the production of defense-related oxidizing enzymes in treated plants. Exposure of aphids to AgNPs-treated plants before virus acquisition reduced BYMV acquisition and transmission efficiency by 40.65 to 100% at 24 h post-application, depending on the AgNP dosage. At 10 days after treatment, virus acquisition was reduced by 36.82% and 79.64% upon exposure to AgNPs at a concentration of 250 and 300 mg L- 1 , respectively. These results suggest that AgNPs have curative viricidal activity due to targeting the virus coat protein and affecting virus-vector interactions. Accordingly, AgNPs may contribute to alleviating the natural disease and virus transmission under field conditions. This is the first report on the activity of nanomaterials against plant virus acquisition and transmission by insects., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)