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1. Implication toward a simple strategy to generate pH tunable FRET-based biosensing

Author

Dipak Kumar, Rana and Subhash Chandra, Bhattacharya

Subjects
Microscopy, Fluorescence, Doxorubicin, Fluorescence Resonance Energy Transfer, Humans, Hep G2 Cells, Hydrogen-Ion Concentration, Instrumentation, Spectroscopy, Atomic and Molecular Physics, and Optics, Analytical Chemistry
Abstract

The present contribution depicts a unique approach to generate tunable Förster resonance energy transfer (FRET) emission with variation of pH of the medium. The pH sensitive absorption of Doxorubicin leads to modification of spectral overlap between emission spectra of donor (Pyrazoline) and absorption spectra of acceptor (Doxorubicin) thereby sensing maximum FRET efficiency in an optimum pH (near pK

Published
2022

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