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In Indonesia, Boesenbergia rotunda, also referred to as Temu kunci. According to earlier research, B. rotunda is a rich source of flavonoids and other phenolic compounds. Extensive research is required to examine the chemical profiles of indigenous plants and analyze medicinal properties from extracts of B. rotunda. Thus, a phytochemical investigation is conducted on the extracts of B. rotunda and two recognized flavonoids, pinostrobin (1) and pinocembrin (2), were isolated from extracts of B. rotunda utilizing a variety of chromatographic methods. The structures are determined using the FTIR, and HPLC, 1H and 13C NMR data and comparison with published literature. The Physicochemical, pharmacokinetic, and toxicity prediction of substances (pKCSM) online tool was also used in this work to estimate the pharmacokinetic and toxicity potencies of pinostrobin and pinocembrin. The pKCSM prediction indicates that pinocembrin and pinostrobin have good pharmacokinetic characteristics and lack the potential to be hepatotoxic or mutagenic substances. Both pinostrobin and pinocembrin have low level lethal dosage values (LD50) of 2000 mol/kg. However, more investigation is still required to validate these hypotheses.

Abstract Gaharu bouya oil obtained from distillation of the woods from Gonystylus genus has attracted essential oil industry interest. However, the information about gaharu bouya essential oil profile is limited. The presence of Gonystylus species is also critically endangered on the IUCN Red List. Therefore, exploring the -omics profiles of Gonystylus bancanus, a native plant from Borneo Island, is important for Indonesia to conserve the population. This research investigated the metabolite profiling of G. bancanus oil, especially the volatile components of its essential oils. Distillations were performed in two technical ways: hydrodistillation on a laboratory scale and steam distillation on an industrial scale. According to LC–MS and GC–MS profiles, both essential oils displayed similar chemical compositions. This article also discusses the similarity of the chemical contents of gaharu bouya oil and agarwood oil from the gaharu superior type (Aquilaria) to support the value of the oil. This research also investigated the cytotoxicity of gaharu bouya oil against three cell lines: HeLa, MCF-7, and HT-29.

Abstract COVID-19 has been a major global health concern for the past three years, and currently we are still experiencing coronavirus patients in the following years. The virus, known as SARS-CoV-2, shares a similar genomic identity with previous viruses such as SARS-CoV and MERS-CoV. To combat the pandemic, modern drugs discovery techniques such as in silico experiments for docking and virtual screening have been employed to design new drugs against COVID-19. However, the release of new drugs for human use requires two safety assessment steps consisting of preclinical and clinical trials. To bypass these steps, scientists are exploring the potential of repurposing existing drugs for COVID-19 treatment. This approach involves evaluating antiviral activity of drugs previously used for treating respiratory diseases against other enveloped viruses such as HPV, HSV, and HIV. The aim of this study is to review repurposing of existing drugs, traditional medicines, and active secondary metabolites from plant-based natural products that target specific protein enzymes related to SARS-CoV-2. The review also analyzes the chemical structure and activity relationship between selected active molecules, particularly flavonol groups, as ligands and proteins or active sites of SARS-CoV-2.

Abstract Stachytarpheta jamaicensis is one of the folk medicines used for the treatment of diabetes in Ambon, Indonesia, but there are limited studies on the bioactivities of its constituents. This study aims to assess the antioxidant and antidiabetic activities of four extracts of S. jamaicensis leaves extracted using several solvents. Bioassay guided fractionation on each extract establishes for exploring S. jamaicensis leaves active compounds. The antioxidant was evaluated using the DPPH and ABTS methods, while the α-glucosidase inhibitory was carried out in vitro assay. The results showed that the methanol extract of S. jamaicensis leaves displays inhibition of DPPH, ABTS and α-glucosidase activity compared to other solvent extracts. Furthermore, 6β-hydroxyipolamiide was successfully isolated from the methanol extract of S. jamicensis leaves which was reported to have α-glucosidase inhibitory activity with an IC50 of 539.17 μg/mL. Based on the results, S. jamaicensis could be recommended as an antioxidant and antidiabetic agent.

Abstract Gonystylus bancanus wood or ramin wood has been generally known as a source of agarwood (gaharu) bouya, a kind of agarwood inferior type, or under the exported trading name of aetoxylon oil. The massive exploitation of ramin wood is causing this plant's extinction and putting it on Appendix II CITES and IUCN Red List of Threatened Species. To date, no scientific publication concerns the chemical exploration of G. bancanus wood and preserving this germplasm through its metabolite profiling. Therefore, research focused on chemical components profiling of G. bancanus is promised. This research is aimed to explore metabolomics and analyze the influence of solvent polarities on the partitioning of metabolites in G. bancanus wood. A range of solvents in different polarities was applied to provide comprehensive extraction of metabolites in G. bancanus wood. Moreover, a hydrodistillation was also carried out to extract the volatile compounds despite the non-volatile ones. LCMS and GCMS analyses were performed to identify volatile and non-volatile components in the extracts and essential oil. Multivariate data analysis was processed using Principal Component Analysis (PCA) and agglomerative hierarchical clustering. 142 metabolites were identified by LCMS analysis, while 89 metabolites were identified by GCMS analysis. Terpenoids, flavonoids, phenyl propanoids, and saccharides are some major compound classes available from LCMS data. Oxygenated sesquiterpenes, especially 10-epi-γ-eudesmol, and β-eudesmol, are the major volatile components identified from GCMS analysis. PCA of LCMS analysis demonstrated that PC1 discriminated two clusters: essential oil, dichloromethane, and n-hexane extracts were in the positive quadrant, while methanol and ethyl acetate extracts were in the negative quadrant. Three-dimensional analysis of GCMS data revealed that n-hexane extract was in the superior quadrant, and its composition can be significantly distinguished from other extracts and essential oil. G. bancanus wood comprises valuable metabolites, i.e., terpenoids, which benefit the essential oil industry. Comprehensive extraction by performing solvents in different polarities on G. bancanus wood could allow exploration of fully extracted metabolites, supported by the exhibition of identified metabolites from LCMS and GCMS analysis.

Mangifera rufocostata, a member of the Mangifera genus, is a source of secondary metabolites and has been used traditionally as antidiabetic agent. Therefore, this study aims to determine the total flavonoid contens (TFC), total phenolic contents (TPC), antioxidant, α-glucosidase inhibitory, and cytotoxic activities of M. rufocostata stem bark extract. Antioxidant activity were evaluated by 2-2″-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing-antioxidant power (FRAP) tests. α-glucosidase inhibitory to prediction antidiabetic activity, and cytotoxic activity were studied against HT-29, HeLa, and MCF-7 cancer cell lines. The results showed that the methanol extract have the highest TFC, TPC, antioxidant, and α-glucosidase inhibitory activity. Furthermore, the methanol extract has strong cytotoxicity againts HT-29 cancer cells. Based on LC-MS/MS, the methanol extract contain several major compounds such as mangiferin, quinic acid, gallic acid, and 3-methoxy-4-hydroxy-phenyl glycol.

Nicotiana tabacum (tobacco) is one of the most commercially agricultural crops in the world. Indonesia is one of the top ten tobacco producing countries in the world since 1990’s. Based on literature studies, tobacco has the main secondary metabolites such as cembranoids and flavonoids. They have fine biological activities as antiviral, antimicrobial, antioxidant, anti-HIV, anti-proliferative, anti-inflammatory, anti-parasitic, anti-termite, and cytotoxicity effects. Therefore, N. tabacum is potential for further investigation on natural products research. This review aims to provide scientific evidences related to structure activity relationship between secondary metabolites and biological activities of N. tabacum.

Based on the amount of discharge or current, the river flows in Central Kalimantan have the potential to produce electrical energy. The purposes of this study were to design an undershot type of floating waterwheel and to test the effective bending angle at the radius of the grasshopper elbow in producing the most optimum power. This research uses experimental methods. The tools used are: mobile phone, multimeter, the gate of light, timer counter, flow rate, and the dimensions of the waterwheel diameter is 6 meters. Grasshopper angles vary from 0°, 30°, 45°, 60o, and 90° with a submerged blade depth of 0.24 m. The results showed that the undershot waterwheel with a flexible pinwheel (like a grasshopper's elbow) produced a faster and more effective rotation than a wheel with a fixed pinwheel and blades. Because the waterwheel has a flexible pinwheel and the butterfly blades experience little resistance when moving in water, the wheel generates more electrical energy than a wheel with fixed pinwheels and blades.At the angle of bending of the radius of the grasshopper blade 30º with the butterfly blade, it produces more optimal electrical energy than angles 0°, 45°, 60o, and 90°. Suggestions for further research are to test the waterwheel in weak and medium current rivers

The nutritional content of mushrooms depends on the type of substrate in the growing medium. Lignocellulose is one of the substrates used for fungal growth media. In this study, the effect of adding dried reeds (Imperata cylindrical (L.) Beauv) on Sengon (Paraserianthes falcataria (L.) Nielsen) sawdust-based media was investigated for wood ear mushroom (Auricularia auricula-Judae) fruiting body. Reeds have been added to Sengon sawdust as wood ear mushroom cultivated medium with variations of S0, S1, S2, S3, and S4 (0%, 25%, 50%, 75%, and 100% of reeds, respectively). S0 was designed as the negative control and S4 as the positive control. The evaluation was carried out against the C/N ratio, yield, biological efficiency (BE), nutritional content, and antioxidant activity of fruiting body mushrooms after 10, 20, and 30 days planted. The highest C/N ratio and BE were obtained from the S1 medium. The lowest moisture content was obtained from the S0 medium. The lowest fat content was obtained from the S4 medium. The best result of nutritional analysis includes the following; the highest total carbohydrate, ash, crude fiber, and crude protein content was obtained from S3, S4, S3, and S0. Furthermore, the best result of antioxidant activity against ABTS and DPPH was obtained at methanol extract of fruiting body mushrooms from S1 medium, compared with ascorbic acid as the positive control. This study has shown that adding reeds to the cultivated medium influences the growth, nutritional content, and antioxidant activity of wood ear mushrooms.

The national history textbook is one of the most controversial textbooks. In books, historical events are presented in the form of narratives that can be used as lessons for the present and future challenges. Narratives can be studied from the point of view of critical discourse analysis that provides awareness. A literature study method with a close reading technique is used to understand the narrative pattern in the textbook. The textbooks in this analysis are textbooks published between 1975-2003. These books represent textbooks from the 1975 curriculum to the 1994 curriculum, supplement to GBPP 1999. The results of the analysis show that this textbook shows a role narrative pattern using a concentric chronological approach, using the principles of accommodative history writing, highlighting aspects of heroism, and using an event approach in the flow of food. The weakness of this textbook is that there are attempts to build an unbalanced role narrative. The narrative of the role of the regime in power at that time was presented with an exaggerated cult tendency. Buku teks sejarah nasional adalah salah satu buku teks yang paling sering mengundang kontroversi. Pada buku teks, setiap kejadian sejarah disajikan sebagai narasi sejarah yang menjadi materi pembelajaran untuk kehidupan masa sekarang dan menghadapi tantangan di masa depan. Sajian narasi dalam analisis buku dapat ditelaah menggunakan pespektif model analisis wacana kritis dengan mengedepankan aspek kesadaran sejarah. Untuk memahami pola narasi dalam buku teks digunakan metode studi literatur dengan teknik close reading. Buku teks dalam analisis ini merupakan buku teks yang diterbitkan antara tahun 1975-2003. Buku-buku tersebut mewakili buku teks dari kurikulum 1975 sampai dengan kurikulum 1994, suplemen GBPP 1999. Hasil analisis menunjukkan bahwa buku teks ini menunjukkan pola narasi peran dengan menggunakan pendekatan kronologis konsentris, menggunakan prinsip penulisan sejarah akomodatif, menonjolkan aspek heroism, dan menggunakan pendekatan peristiwa dalam alur sajiannya. Kelemahan buku teks ini adalah terdapat upaya membangun narasi peran yang tidak seimbang. Narasi peran bagi rezim yang sedang berkuasa pada saat itu ditampilkan dengan tendensi pengkultusan yang berlebihan.

The oyster mushroom (Pleurotus ostreatus) is the most cultivated mushroom which its growth performance and nutritional composition depend on substrate types. Thus, this study investigated the effect of coconut coir on minerals and vitamins B contents in the oyster mushroom cultivation medium. The composition of coconut coir and Sengon’s sawdust as growth medium were variated at ratios of 4:0 (F1), 3:1 (F2), 2:2 (F3), 1:3 (F4), 0:4 (F5) (w/w). Furthermore, the mineral and vitamin B contents of the harvested oyster mushrooms were analyzed by ICPMS and LCMS, respectively. The highest minerals contents of potassium 26,909 mg/kg, 1,136 mg/kg of phosphorus, 313 mg/kg of magnesium, 4,346 mg/kg of calcium, 15.4 mg/kg for zinc, 2.07 mg/kg of copper, 0.623 mg/kg of Selenium were identified at F1, whereas the highest manganese was at F2 (15.3 mg/kg). Potassium was the highest mineral content in oyster mushrooms in all growth media, and low toxic minerals content of cadmium was detected between 0.015-0.058 mg/kg in all variant substrates. Lead (0.525 mg/kg) and mercury (0.012 mg/kg) was only detected at F1, while arsenic was detected at F4 (0.002 mg/kg) and F5 (0.029 mg/kg). LCMS analysis showed that vitamin B1 (Thiamine), B3 (niacin), B6 (pyridoxine) and B12 (cyanocobalamin) were detected in all growth media, while vitamin B5 (pantothenic acid) was only detected at F5. These results indicated that the P. ostreatus cultivation on coconut coir become valuable nutritional resources to alleviate malnutrition and help dispose of coconut coir in an environment-friendly manner.