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A case of polyparasitism in an 8-year-old female cat from Southern Brazil is reported. Among the described clinical signs, both gurltiosis and lagochilascariasis are of rare occurrence, being categorized as neglected diseases. The diagnosis of feline crural parasitic paraplegia was established through clinical signs, necropsy lesion observations, and the presence of Gurltia paralysans in histological sections of the spinal cord. Lagochilascaris minor was diagnosed with an ulcerated wound in the cervical region and further confirmed by coprology. In addition, other gastrointestinal parasites (i.e., Trichuris spp., Diphyllobothriidae, Toxocara cati, Ancylostoma spp., Cystoisospora spp., and Entamoeba spp.) were diagnosed through fecal examination and Sarcocystis felis by molecular analysis of muscle cysts. Aelurostrongylus abstrusus was detected in histological sections of lung parenchyma and confirmed by molecular analyses. The multiple parasitism detected in this case represents a unique report in terms of the number of parasites in the same individual, indicating the risk represented by stray cats as spreaders of zoonotic agents., Competing Interests: Declarations. Ethical approval: Not applicable. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Introduction: In soccer, most studies evaluate metabolic profile changes in male athletes, often using data from a single match. Given the current landscape of women's soccer and the effects of biological sex on the physiological response and adaptation to exercise, more studies targeting female athletes and analyzing pre- and post-game moments throughout the season are necessary., Objectives: To describe the metabolomics profile of female soccer athletes from an elite team in Brazil. The study observed the separation of groups in three pre- and post-game moments and identified the discriminating metabolites., Methods: The study included 14 female soccer athletes. Urine samples were collected and analyzed using Nuclear Magnetic Resonance in pre-game and immediate post-game moments over three national championship games. The metabolomics data were then used to generate OPLS-DA and VIP plots., Results: Forty-three metabolites were identified in the samples. OPLS-DA analyses demonstrated a progressive separation between pre-post conditions, as supported by an increasing Q 2 value (0.534, 0.625, and 0.899 for games 1, 2 and 3, respectively) and the first component value (20.2% and 19.1% in games 1 and 2 vs. 29.9% in game 3). Eight out of the fifteen most discriminating metabolites appeared consistently across the three games: glycine, formate, citrate, 3-hydroxyvalerate, glycolic acid, trimethylamine, urea, and dimethylglycine., Conclusion: The main difference between the three games was the increasing separation between groups throughout the championship. Since the higher VIP-scores metabolites are linked to energy and protein metabolism, this separation may be attributed several factors, one being the accumulation of fatigue., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Background: The benefits of caffeine to physical performance have been extensively demonstrated, however, it has recently been speculated that there is an effect of the administration route on its effectiveness. Purpose: The current study investigated the effect of caffeine mouth rinse in isolation or combined with ingestion on performance in a 30-minute constant-load exercise followed by a 10-km cycling time trial. Methods: Ten physically active men performed a 30-minute constant-load exercise at 50% of the graded test Wmax, followed by a 10-km cycling time trial. Before and at the middle points of the constant-load exercise and 10-km cycling time trial, the following conditions were administered: PLA (cellulose ingestion plus mouth rinsing with magnesium sulfate), ING (5 mg.kg -1 of caffeine ingestion plus mouth rinsing with magnesium sulfate), MR (cellulose ingestion plus mouth rinsing with 1.2% caffeine), and COMB (5 mg.kg -1 of caffeine ingestion plus mouth rinsing with 1.2% caffeine). Results: During the 30-minute constant-load exercise, COMB presented a lower rating of perceived exertion (RPE) than MR ( p  = .04). For the 10-km time trial, the COMB was faster than MR (MR = 1363 ± 345 vs. COMB = 1291 ± 308s, Δ% = 5.57, p  = .05). Mean power output was higher in COMB than PLA, ING, and MR (234 ± 15 vs. 169 ± 29, 148 ± 11, and 145 ± 12 W, respectively). There were no differences between conditions for heart rate and RPE during the 10-km time trial. Conclusion: In summary, caffeine mouth rinsing potentiated the effects of caffeine ingestion during the 10-km time trial compared to caffeine mouth rinsing alone.

The aim was to investigate the long-term effects of metformin ingestion on high-intensity interval training on performance, glycogen concentration (GC), GLUT-4 content, and metabolomics outcomes in rats. Fifty male Wistar rats were randomly divided into baseline, metformin (500 mg daily), and control groups. Training consisted of 4 sets of 10 jumps with 30 s of passive recovery per day, 5 days/week for 8 weeks. The intensity equivalent was 50% of body mass (BM) in the first four weeks and 70% of BM in the last four weeks. The animals were submitted to a weekly jump test until exhaustion at 50% of BM. Serum and tissues were collected at baseline and after 4 and 8 weeks for biochemical and metabolomics analysis. The number of jumps increased in the Control group without a significant difference between groups at 4 and 8 weeks. GLUT4 was lower in the gastrocnemius muscle in the Metformin at the fourth week compared to Control (P=0.03) and compared to Metformin (P=0.02) and Control (P=0.01) at eight weeks. Hepatic and soleus GC were not altered by metformin. Gastrocnemius GC was lower after 8 weeks in the Metformin group compared to Control (P=0.01). Significantly lower levels of pyruvate and phenylalanine and higher levels of ethanol, formate, betaine, very low-density lipoprotein, low-density lipoprotein, and creatine were found in the Metformin compared to the Control. Although chronic administration of metformin decreased food intake and negatively influenced the synthesis of muscle glycogen, it did not significantly change physical performance compared to the Control.

We analyzed the effects of load magnitude and bar velocity variables on sensitivity to fatigue. Seventeen resistance-trained men (age=25.7±4.9 years; height=177.0±7.2 cm; body mass=77.7±12.3 kg; back-squat 1RM=145.0±33.9 kg; 1RM/body mass=1.86) participated in the study. Pre- and post-exercise changes in the mean propulsive velocity (MPV) and peak velocity (PV) in the back-squat at different intensities were compared with variations in the countermovement jump (CMJ). CMJ height decreased significantly from pre- to post-exercise (∆%=-7.5 to -10.4; p<0.01; ES=0.37 to 0.60). Bar velocity (MPV and PV) decreased across all loads (∆%=-4.0 to -12.5; p<0.01; ES=0.32 to 0.66). The decrease in performance was similar between the CMJ, MPV (40% and 80% 1RM; p=1.00), and PV (80% 1RM; p=1.00). The magnitude of reduction in CMJ performance was greater than MPV (60% 1RM; p=0.05) and PV (40% and 60% 1RM; p<0.01) at the post-exercise moment. Low systematic bias and acceptable levels of agreement were only found between CMJ and MPV at 40% and 80% 1RM (bias=0.35 to 1.59; ICC=0.51 to 0.71; CV=5.1% to 8.5%). These findings suggest that the back-squat at 40% or 80% 1RM using MPV provides optimal sensitivity to monitor fatigue through changes in bar velocity., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)

There is a lack of evidence on the additional benefits of combining caffeine (CAF) and creatine (CRE) supplementation on anaerobic power and capacity. Thus, the aim of the present study was to test the effects of combined and isolated supplementation of CAF and CRE on anaerobic power and capacity. Twenty-four healthy men performed a baseline Wingate anaerobic test and were then allocated into a CRE (n = 12) or placebo (PLA; n = 12) group. The CRE group ingested 20 g/day of CRE for 8 days, while the PLA group ingested 20 g/day of maltodextrin for the same period. On the sixth and eighth days of the loading period, both groups performed a Wingate anaerobic test 1 hr after either CAF (5 mg/kg of body mass; CRE + CAF and PLA + CAF conditions) or PLA (5 mg/kg of body mass of cellulose; CRE + PLA and PLA + PLA conditions) ingestion. After the loading period, changes in body mass were greater (p < .05) in the CRE (+0.87 ± 0.23 kg) than in the PLA group (+0.13 ± 0.27 kg). In both groups, peak power was higher (p = .01) in the CAF (1,033.4 ± 209.3 W) than in the PLA trial (1,003.3 ± 204.4 W), but mean power was not different between PLA and CAF trials (p > .05). In conclusion, CAF, but not CRE ingestion, increases anaerobic power. Conversely, neither CRE nor CAF has an effect on anaerobic capacity.

Non-communicable diseases (NCDs) are the major cause of death worldwide and have economic, psychological, and social impacts. Air pollution is the second, contributing to NCDs-related deaths. Metabolomics are a useful diagnostic and prognostic tool for NCDs, as they allow the identification of biomarkers linked to emerging pathologic processes. The aim of the present study was to review the scientific literature on the application of metabolomics profiling in NCDs and to discuss environmental planning actions to assist healthcare systems and public managers based on early metabolic diagnosis. The search was conducted following PRISMA guidelines using Web of Science, Scopus, and PubMed databases with the following MeSH terms: "metabolomics" AND "noncommunicable diseases" AND "air pollution". Twenty-nine studies were eligible. Eleven involved NCDs prevention, eight addressed diabetes mellitus, insulin resistance, systemic arterial hypertension, or metabolic syndrome. Six studies focused on obesity, two evaluated nonalcoholic fatty liver disease, two studied cancer, and none addressed chronic respiratory diseases. The studies provided insights into the biological pathways associated with NCDs. Understanding the cost of delivering care where there will be a critical increase in NCDs prevalence is crucial to achieving universal health coverage and improving population health by allocating environmental planning and treatment resources.

This study aimed to test the agreement of the incremental test's physiological responses between tethered running on a nonmotorized treadmill (NMT) to matched relative intensities while running on a conventional motorized treadmill (MT). Using a within-subject crossover design, nine male recreational runners (age = 22 ± 5 years; height = 175 ± 6 cm; weight = 68.0 ± 16.6 kg) underwent two test sessions: one was an incremental intensity protocol on an MT; the other was on an instrumented NMT. Intensity thresholds at [Formula: see text]O 2max , respiratory compensation point (iRCP), and lactate threshold (iLT) were registered for analysis, together with [Formula: see text]̇O 2 , [Formula: see text]̇E, ƒ R, and blood lactate concentration ([Lac]). Comparisons were based on hypothesis testing (Student's T-test), effect sizes (Cohen's d), ICC, and Bland Altman analysis. Statistical significance was accepted at p < 0.05. Attained [Formula: see text]O 2max (MT = 52.2 ± 7.3 mL·kg -1 ·min -1 vs NMT = 50.1 ± 8.1 mL·kg -1 ·min -1 ) and [Formula: see text]̇O 2 at iRCP (MT = 46.3 ± 7.2 mL·kg -1 ·min -1 vs NMT = 42.8 ± 9.3 mL·kg -1 ·min -1 ) were not different between ergometers (p = 0.15 and 0.13, respectively), with significant ICCs (0.84 and 0.70, respectively) and Pearson's correlations (r = 0.87 and 0.76, respectively). The [Lac] at iLT presented poor agreement between conditions. Significant correlations were found (r between 0.72 and 0.83) for relative power values of i[Formula: see text]O 2max (6.56 ± 1.28 W·kg -1 ), iRCP (4.38 ± 1.50 W·kg -1 ), and iLT (4.15 ± 1.29 W·kg -1 ) related to their counterpart obtained on MT. Results show that running on an NMT offers a higher glycolytic demand under the same relative internal load as running on an MT but with a similar aerobic response and correlated intensity determination., (© 2022. The Author(s).)

This study aimed to compare four constructs from the three-minute all-out test (AO3)-end power (EP), the area above EP (WEP), maximum power (Pmax), and attained [Formula: see text]-to those derived from the classical CP model in tethered running. Seventeen male recreational runners underwent two experiments to test for reliability and agreement of AO3 parameters with those obtained from the classical CP model (Wꞌ and CP), a graded exercise test ([Formula: see text]) and a 30-second all-out test (AO30s; Pmax); all performed on a non-motorized treadmill (NMT). Significance levels were set at p<0.05. There were no significant differences between test-retest for Pmax (p = 0.51), WEP (p = 0.39), and EP (p = 0.64), showing generally close to zero bias. Further, retest ICC were high for Pmax and EP (ICC > 0.86) but moderate for WEP (ICC = 0.69). Pmax showed no difference between AO3 and AO30s (p = 0.18; CV% = 9.5%). EP and WEP disagreed largely with their classical critical power model counterparts (p = 0.05; CV%>32.7% and p = 0.23; CV%>39.7%, respectively), showing greater error than their test-retest reliability. [Formula: see text] from AO3 was not different (p = 0.13) and well related (CV% = 8.4; ICC = 0.87) to the incremental test [Formula: see text]. Under the studied conditions, the agreement of EP and WEP to CP and Wꞌ was not strong enough to assure their use interchangeably. Pmax and [Formula: see text] were closer to their criterion parameters., Competing Interests: The authors have declared that no competing interests exist.

Purpose: The study aimed to assess the metabolic impact of elite Brazilian U-20 players using the rating of perceived exertion scale (RPE) to discriminate metabolomics sensitivity post-two soccer games separated by a short recovery interval., Methods: Urine was collected immediately and then 20 h after two soccer matches of elite Brazilian U-20 players. RPE was collected after games. The spectra were pre-processed using TopSpin ® 3.2 software. Chenomx ® software was used to identify metabolites in the urine through the available database., Results: The results showed that the metabolic pathways related to energy production, cellular damage, and organic stresses were changed immediately after the game. 20 h after the games, antioxidant and anti-inflammatory pathways related to cell recovery were identified (e.g., gallic acid, ascorbate, and betaine). The matrix of positive correlations between metabolites was more predominant and stronger after game 2 than game 1. T-distribution registered metabolites discriminated below and above 7 on the RPE scale. Athletes with higher RPE values showed a high metabolite profile related to muscle damage (e.g., creatine, creatinine, and glycine) and energy production (e.g., creatine, formate, pyruvate, 1,3 dihydroxyacetone) 20 h post-soccer match. There was a different metabolic profile between athletes with higher and lower RPE values., Conclusion: Metabolomics analysis made it possible to observe the metabolic impacts of energy production and muscular damage. RPE identified internal load changes within the group as a result of match intensity in soccer. The correlation matrix indicated a greater predominance of positive and strong correlations between metabolites in the second game compared to the first game., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)