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This article discusses the engineering of soybean plants with high levels of herbicide resistance using a Cas12-SF01-based cytosine base editor. Non-transgenic herbicide-tolerant soybean varieties are in demand due to the challenges posed by weeds in soybean cultivation. The study explores the use of amino acid substitution mutations in multiple ALS genes to produce soybeans with sufficient herbicide resistance. The researchers successfully generated herbicide-resistant soybean mutants and evaluated their resistance in greenhouse and field experiments. The results showed that the mutants exhibited strong herbicide resistance without affecting plant productivity. This study demonstrates the effectiveness of the base editor system for breeding herbicide-tolerant soybeans. [Extracted from the article]

A recent article in the Journal of Integrative Plant Biology discusses a simple method for transforming and editing genes in medicinal plants. The method, called Cut-Dip-Budding (CDB), bypasses the need for tissue culture and hairy root formation, making it more efficient and successful than traditional gene delivery methods. The researchers successfully used the CDB method to transform and edit genes in several medicinal plant species, including Pugongying, Dihuang, Danshen, and Yuanzhi. This breakthrough has the potential to greatly expand the number of medicinal plants that can be genetically modified and studied. [Extracted from the article]

Nano‐reduced iron (NRI) is a promising uranium adsorbent due to its strong reducibility and good selectivity, but it still faces the challenges of slow kinetics, limited and non‐renewable active sites. In this work, we realized high efficiency uranium extraction under ultra‐low cell voltage (−0.1 V) in seawater with 20 ppm UO2(NO3)2 solution by coupling electrochemical mediated FeII/FeIII redox and uranium extraction. The adsorption capacity and extraction efficiency of NRI after electrochemical uranium extraction (EUE) could reach 452 mg/g and 99.1 %, respectively. Combined with quasi‐operando/operando characterization technologies, we clarified the mechanism of EUE and revealed that continuously regenerating FeII active sites by electroreduction could significantly enhance the property of EUE. This work here provides a new electrochemical mediated and low energy consumption uranium extraction strategy which also provides a reference for other metal resource recovery. [ABSTRACT FROM AUTHOR]

Nickel hydroxide (Ni(OH)2) is a promising electrocatalyst for the 5‐hydroxymethylfurfural oxidation reaction (HMFOR) and the dehydronated intermediates Ni(OH)O species are proved to be active sites for HMFOR. In this study, Ni(OH)2 is modified by platinum to adjust the electronic structure and the current density of HMFOR improves 8.2 times at the Pt/Ni(OH)2 electrode compared with that on Ni(OH)2 electrode. Operando methods reveal that the introduction of Pt optimized the redox property of Ni(OH)2 and accelerate the formation of Ni(OH)O during the catalytic process. Theoretical studies demonstrate that the enhanced Ni(OH)O formation kinetics originates from the reduced dehydrogenation energy of Ni(OH)2. The product analysis and transition state simulation prove that the Pt also reduces adsorption energy of HMF with optimized adsorption behavior as Pt can act as the adsorption site of HMF. Overall, this work here provides a strategy to design an efficient and universal nickel‐based catalyst for HMF electro‐oxidation, which can also be extended to other Ni‐based catalysts such as Ni(HCO3)2 and NiO. [ABSTRACT FROM AUTHOR]

Introduction: The incidence of anastomotic leakage is relatively high (6-26%) in esophagogastrocervical anastomosis. The traditional anastomotic mode has high tissue tension in the process of anastomosis, which can easily cause tissue tear in the anastomotic area and increase the incidence of anastomotic leakage., Aim: To investigate the usefulness of reversely connecting circular stapler technique for reducing anastomotic tension and injury of the esophageal and gastric wall, preventing anastomotic leakage in minimally invasive McKeown esophagectomy., Material and Methods: A total of 133 patients with esophageal cancer who underwent minimally invasive McKeown esophagectomy from January 2021 to December 2021 were collected. Characteristics, surgical data, and outcomes of patients were recorded and retrospectively analyzed. There were 83 patients in the reverse order connecting circular stapler group and 50 patients in the conventional order connecting group., Results: Anastomotic leakage was observed in 2 (2.4%) cases in the reverse order connecting circular stapler group. Compared with the conventional connection group, the reverse connecting group had lower incidence of anastomotic leakage, shorter anastomotic time, shorter hospital stay, and lower incidence of pulmonary and chest infections and hoarseness., Conclusions: The reversely connecting circular stapler technique could reduce the incidence of anastomotic leakage. The technique is simple to perform and easy to learn. Therefore, it is useful for the prevention of anastomotic leakage., Competing Interests: The authors declare no conflict of interest., (Copyright © 2023 Sekcja Wideochirurgii TChP.)

Nano-reduced iron (NRI) is a promising uranium adsorbent due to its strong reducibility and good selectivity, but it still faces the challenges of slow kinetics, limited and non-renewable active sites. In this work, we realized high efficiency uranium extraction under ultra-low cell voltage (-0.1 V) in seawater with 20 ppm UO 2 (NO 3 ) 2 solution by coupling electrochemical mediated Fe II /Fe III redox and uranium extraction. The adsorption capacity and extraction efficiency of NRI after electrochemical uranium extraction (EUE) could reach 452 mg/g and 99.1 %, respectively. Combined with quasi-operando/operando characterization technologies, we clarified the mechanism of EUE and revealed that continuously regenerating Fe II active sites by electroreduction could significantly enhance the property of EUE. This work here provides a new electrochemical mediated and low energy consumption uranium extraction strategy which also provides a reference for other metal resource recovery., (© 2023 Wiley-VCH GmbH.)

Of the more than 370 000 species of higher plants in nature, fewer than 0.1% can be genetically modified due to limitations of the current gene delivery systems. Even for those that can be genetically modified, the modification involves a tedious and costly tissue culture process. Here, we describe an extremely simple cut-dip-budding (CDB) delivery system, which uses Agrobacterium rhizogene to inoculate explants, generating transformed roots that produce transformed buds due to root suckering. We have successfully used CDB to achieve the heritable transformation of plant species in multiple plant families, including two herbaceous plants ( Taraxacum kok-saghyz and Coronilla varia ), a tuberous root plant (sweet potato), and three woody plant species ( Ailanthus altissima , Aralia elata , and Clerodendrum chinense ). These plants have previously been difficult or impossible to transform, but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protocol, under non-sterile conditions and without the need for tissue culture. Our work suggests that large numbers of plants could be amenable to genetic modifications using the CDB method., Competing Interests: The authors declare no competing interests., (© 2022 The Authors.)

Nickel hydroxide (Ni(OH) 2 ) is a promising electrocatalyst for the 5-hydroxymethylfurfural oxidation reaction (HMFOR) and the dehydronated intermediates Ni(OH)O species are proved to be active sites for HMFOR. In this study, Ni(OH) 2 is modified by platinum to adjust the electronic structure and the current density of HMFOR improves 8.2 times at the Pt/Ni(OH) 2 electrode compared with that on Ni(OH) 2 electrode. Operando methods reveal that the introduction of Pt optimized the redox property of Ni(OH) 2 and accelerate the formation of Ni(OH)O during the catalytic process. Theoretical studies demonstrate that the enhanced Ni(OH)O formation kinetics originates from the reduced dehydrogenation energy of Ni(OH) 2 . The product analysis and transition state simulation prove that the Pt also reduces adsorption energy of HMF with optimized adsorption behavior as Pt can act as the adsorption site of HMF. Overall, this work here provides a strategy to design an efficient and universal nickel-based catalyst for HMF electro-oxidation, which can also be extended to other Ni-based catalysts such as Ni(HCO 3 ) 2 and NiO., (© 2021 Wiley-VCH GmbH.)