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Tiller number and grain size are important agronomic traits that determine grain yield in rice. Here, we demonstrate that DEFECTIVE TILLER GROWTH 1 (DTG1), a member of the casein kinase 1 protein family, exerts a co-regulatory effect on tiller number and grain size. We identified a single amino acid substitution in DTG1 (I357K) that caused a decrease in tiller number and an increase in grain size in NIL-dtg1. Genetic analyses revealed that DTG1 plays a pivotal role in regulation of tillering and grain size. The DTG1I357K allelic variant exhibited robust functionality in suppressing tillering. We show that DTG1 is preferentially expressed in tiller buds and young panicles, and negatively regulates grain size by restricting cell proliferation in spikelet hulls. We further confirm that DTG1 functioned in grain size regulation by directly interacting with Grain Width 2 (GW2), a critical grain size regulator in rice. The CRISPR/Cas9-mediated elimination of DTG1 significantly enhanced tiller number and grain size, thereby increasing rice grain yield under field conditions, thus highlighting potential value of DTG1 in rice breeding.

The utilization of heavy-panicle hybrid rice exemplifies the successful integration of architectural enhancement and heterosis, which has been widely adopted in the southwest rice-producing area of China. Iterative improvement in disease resistance and grain quality of heavy-panicle hybrid rice varieties is crucial to promote their sustainable utilization. Here, we performed a molecular design breeding strategy to introgress beneficial alleles of broad-spectrum disease resistance and grain quality into a heavy-panicle hybrid backbone restorer line Shuhui 600 (R600). We successfully developed introgression lines through marker-assisted selection to pyramid major genes (Wxb + ALKA-GC + Pigm + Xa23) derived from three parents (Huanghuazhan, I135, I488), which significantly enhance grain quality and confer resistance to rice blast and bacterial blight (BB). The improved parental R600 line (iR600) exhibited superior grain quality and elevated disease resistance while maintaining the heavy-panicle architecture and high-yield capacity of R600. Moreover, the iR600 was crossed with male sterility line 608A to obtain a new heavy-panicle hybrid rice variety with excellent eating and cooking quality (ECQ) and high yield potential. This study presents an effective breeding strategy for rice breeders to expedite the improvement of grain quality and disease resistance in heavy-panicle hybrid rice.

Calcium sulphoaluminate cement (CSA) is considered a sustainable alternative to traditional Portland cement to reduce CO2 emissions. To further improve the hardening properties and water resistance of CSA and advance its extensive special engineering application, nanoparticles including nano-SiO2 (NS), nano-MgO (NM) and nano-Al2O3 (NA) were used as nano-modifiers for CSA. The effects of nanoparticles dosage on the setting time, compressive strength and water resistance of nano-modified CSA were experimentally evaluated, and the micro-mechanisms were also clarified by conducting X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TG) tests. The results showed that incorporating nanoparticles into CSA obviously shortened the initial and final setting times. The appropriate addition of nanoparticles effectively enhanced the compressive strength of CSA, and the optimum dosages of NS, NM, and NA were respectively determined to be 0.5 %, 1.0 %, and 0.5 %. Water immersion decreased the bonding strength of CSA, and adding nanoparticles can effectively weaken this water-induced deterioration effect. Under water immersion environment, the optimum dosages of nanoparticles would be increased to obtain the highest residual water immersion strength and minimum strength loss rate. The generation of ettringite was mainly responsible for the compressive strength and water resistance improvements of nano-modified CSA. The hydration process of nano-modified CSA was clarified and the corresponding micro-mechanism model was proposed. Furthermore, the benefits and challenges of using nano-modified CSA were also analyzed, advancing its widespread application in rapid repair of special engineering.

The state of health (SOH) and remaining useful life (RUL) of lithium-ion batteries are crucial for health management and diagnosis. However, most data-driven estimation methods heavily rely on scarce labeled data, while traditional transfer learning faces challenges in handling domain shifts across various battery types. This paper proposes an enhanced vision-transformer integrating with semi-supervised transfer learning for SOH and RUL estimation of lithium-ion batteries. A depth-wise separable convolutional vision-transformer is developed to extract local aging details with depth-wise convolutions and establishes global dependencies between aging information using multi-head attention. Maximum mean discrepancy is employed to initially reduce the distribution difference between the source and target domains, providing a superior starting point for fine-tuning the target domain model. Subsequently, the abundant aging data of the same type as the target battery are labeled through semi-supervised learning, compensating for the source model's limitations in capturing target battery aging characteristics. Consistency regularization incorporates the cross-entropy between predictions with and without adversarial perturbations into the gradient backpropagation of the overall model. In particular, across the experimental groups 13–15 for different types of batteries, the root mean square error of SOH estimation was less than 0.66 %, and the mean relative error of RUL estimation was 3.86 %. Leveraging extensive unlabeled aging data, the proposed method could achieve accurate estimation of SOH and RUL.

Objective: The present study aims to evaluate the impact of Astilbin (AST) on cortical neuron survival in vitro under conditions of oxygen-glucose deprivation and reoxygenation (OGD/R) and determine the role of NF-E2-related factor 2 (Nrf2) in this process. Methods: Primary neurons were pre-treated with various concentrations of AST for 8 h before OGD induction. Cell viability and lactate dehydrogenase (LDH) leakage were assessed to determine the optimal concentration. Biomarkers related to oxidative stress, antioxidant enzyme activities, and apoptosis were evaluated at 24 h post-OGD/R. To investigate the involvement of Nrf2 in AST-mediated neuroprotection, we conducted molecular docking and microscale thermophoresis analyses, as well as examined the expression levels of Nrf2 and its regulatory genes including heme oxygenase-1(HO-1), (NAD(P)H: quinone oxidoreductase 1 (NQO-1), and peroxiredoxin 1 (Prdx1). Additionally, lentivirus-mediated knockdown of Nrf2 and overexpression of Nrf2 with L-sulforaphane (SFN) were performed, followed by an assessment of cell viability, oxidative stress, antioxidant enzyme activities and apoptosis. Results: Pre-treatment with AST reduced oxidative stress levels while increasing antioxidant enzyme activities and mitigating neuronal apoptosis. After OGD/R exposure, AST upregulated nuclear Nrf2 expression and increased the expression of HO-1, NQO-1 and Prdx1 in the cytoplasm. However, the knockdown of Nrf2 abolished the antioxidative and protective effects exerted by AST treatment. Conversely, combining AST with the Nrf2 agonist SFN demonstrated an enhancement in the protective effects provided by AST. These results demonstrate that Nrf2-dependent antioxidant responses contribute to AST-induced tolerance against neuronal injury caused by OGD/R injury. Conclusions: Overall findings support the ability of AST to protect primary neurons from OGD/R-induced damage through activation of Nrf2-dependent antioxidant responses.

Abstract To reveal the mechanism of the influence of the curing temperature on the strength of lime activated fly ash-GGBS cured silt soil, the curing of dredged silt was carried out by using fly ash and GGBS as the curing agent and lime as the activator. Unconfined compressive strength (UCS) experiments were carried out, and the micro-analysis of the cured silt was carried out by experimental methods including scanning electron microscope (SEM) tests, X-ray diffraction (XRD), etc. to reveal the mechanism of the curing temperature on the dredged silt. According to the test results, the hydration reaction and pozzolanic reaction between lime-fly ash-GGBS and silt soil were promoted with the increase of the curing temperature. when the curing temperature of the sample reached 40 ℃, a large amount of gel products such as hydrated calcium aluminate (C–A–H) and hydrated calcium silicate (C–S–H) were generated, which enhanced the bonding force between soil particles and filled up the inter-particle pore space, thereby improving the UCS of the sample. The results of SEM confirmed that C–A–H and C–S–H were the main substances for the construction of cured silt skeleton. C–S–H and C–A–H were detected by XRD. The results of the study fill the gap in the effect of curing temperature on the direction of lime-activated fly ash-GGBS cured silt soil.

Glycophagy has evolved from an alternative glycogen degradation pathway into a multifaceted pivot to regulate cellular metabolic hemostasis in peripheral tissues. However, the pattern of glycophagy in the brain and its potential therapeutic impact on ischemic stroke remain unknown. Here, we observed that the dysfunction of astrocytic glycophagy was caused by the downregulation of the GABA type A receptor-associated protein like 1 (GABARAPL1) during reperfusion in ischemic stroke patients and mice. PI3K-Akt pathway activation is involved in driving GABARAPL1 downregulation during cerebral reperfusion. Moreover, glycophagy dysfunction-induced glucosamine deficiency suppresses the nuclear translocation of specificity protein 1 and TATA binding protein, the transcription factors for GABARAPL1, by decreasing their O-GlcNAcylation levels, and accordingly feedback inhibits GABARAPL1 in astrocytes during reperfusion. Restoring astrocytic glycophagy by overexpressing GABARAPL1 decreases DNA damage and oxidative injury in astrocytes and improves the survival of surrounding neurons during reperfusion. In addition, a hypocaloric diet in the acute phase after cerebral reperfusion can enhance astrocytic glycophagic flux and accelerate neurological recovery. In summary, glycophagy in the brain links autophagy, metabolism, and epigenetics together, and glycophagy dysfunction exacerbates reperfusion injury after ischemic stroke.

Abstract Background Lipid metabolism has a crucial role in neural repair in neurodegenerative diseases. We recently revealed that lipogenesis-mediated interleukin-33 (IL-33) upregulation lead to blood–brain barrier (BBB) repair after ischemic stroke. However, manipulating the key enzyme fatty acid synthase (FASN) to enhance lipogenesis was very challenging. Glyceryl triacetate (GTA) was used as a donor of acetate and precursor of acetyl coenzyme A, the key substrate for de novo lipogenesis catalyzed by FASN. Therefore, we hypothesized that GTA would promote lipogenesis the peri-infarct after ischemic stroke and contribute to the BBB repair through IL-33. Methods Middle cerebral artery occlusion (MCAO) was performed on C57BL mice and GTA was gavage administrated (4 g/kg) on day 2 and 4 after MCAO. Lipogenesis was evaluated by assessment of the protein level of FASN, lipid droplets, and fatty acid products through liquid chromatography-mass spectrometry in the peri-infarct area on day 3 after MCAO, respectively. BBB permeability was determined by extravasation of Evans blue, IgG and dextran, and levels of tight junction proteins in the peri-infarct area on day 7 after MCAO, respectively. Infarct size and neurological defects were assessed on day 7 after MCAO. Brain atrophy on day 30 and long-term sensorimotor abilities after MCAO were analyzed as well. The inhibitor of FASN, C75 and the virus-delivered FASN shRNA were used to evaluate the role of FASN-driven lipogenesis in GTA-improved BBB repair. Finally, the therapeutic potential of recombinant IL-33 on BBB repair and neurological recovery was evaluated. Results We found that treatment with GTA increased the lipogenesis as evidenced by lipid droplets level and lauric acid content, but not the FASN protein level. Treatment with GTA increased the IL-33 level in the peri-infarct area and decreased the BBB permeability after MCAO. However, infarct size and neurological defect score were unchanged on day 7 after MCAO, while the long-term recovery of sensorimotor function and brain atrophy were improved by GTA. Inhibition of lipogenesis using C75 or FASN shRNA reversed the beneficial effect of GTA. Finally, exogenous IL-33 improved BBB repair and long-term functional recovery after stroke. Conclusion Collectively, we concluded that treatment with GTA improved the BBB repair and functional recovery after ischemic stroke, probably by the enhancement of lipogenesis and IL-33 expression. Graphical Abstract

Cement-based solidification is a widely used sustainable technology for transforming waste dredged sludge (DS) into backfill materials. This study developed a novel solidification/oxidization synergistic (SOS) method, using Portland cement as a binder and sodium persulfate (SP) as an oxidant, for treating the organic DS with different fulvic acid (FA) content. The results demonstrated that adding SP could significantly improve the UCS of the cement-solidified DS doped with FA. When the FA content was 4%, the 28-day UCS of the sample with 25% cement content was only 160.5 kPa. The addition of 6% SP increased the UCS to 2225.5 kPa, which was 15.1 times that of the sample without SP. However, excessive SP hydrolyzed to produce a large amount of sulfuric acid, leading to a low pH value of the pore solution. This in turn hindered further persulfate activation and cement hydration, which was detrimental to the strength of cement-solidified DS.

Shank3, a key molecule related to the development and deterioration of autism, has recently been found to downregulate in the murine brain after ischemia/reperfusion (I/R). Despite this discovery, however, its effects on neuronal injury and the mechanism underlying the effects remain to be clarified. To address this, in this study, based on genetically modified mice models, we revealed that the expression of Shank3 showed a time-dependent change in murine hippocampal neurons after I/R, and that conditional knockout (cko) of Shank3 in neurons resulted in aggravated neuronal injuries. The protective effects of Shank3 against oxidative stress and inflammation after I/R were achieved through direct binding STIM1 and subsequent proteasome-mediated degradation of STIM1. The STIM1 downregulation induced the phosphorylation of downstream Nrf2 Ser40, which subsequently translocated to the nucleus, and further increased the expression of antioxidant genes such as NQO1 and HO-1 in HT22 cells. In vivo, the study has further confirmed that double knockout of Shank3 and Stim1 alleviated oxidative stress and inflammation after I/R in Shank3cko mice. In conclusion, the present study has demonstrated that Shank3 interacts with STIM1 and inhibits post-I/R neuronal oxidative stress and inflammatory response via the Nrf2 pathway. This interaction can potentially contribute to the development of a promising method for I/R treatment.

A composite material was developed using carbide slag, water glass, slag, and micron silicon to facilitate the use of industrial waste resources. The mechanical properties of dredge sludge (DS) were analyzed, considering different proportions of cement, organic debris, and carbide slag. The composition and microstructure of the hydration products were analyzed using the X-ray diffractometer (XRD), scanning electron microscopy (SEM), and thermogravimetric (TG) analysis. The results indicate that with a precursor content of 20%, a water glass content of 3%, and an increase in carbide slag content from 4% to 12%, the strength of the sample initially increases and subsequently drops at each age. With a carbide slag level of 8%, the combination of CaO in the slag and water glass stimulated the slag and micron silica, leading to the formation of gel substances such C-S-H and C-A-S-H. The soil particles exhibited increased density as a result of the cohesive properties of the gel products. Following a maintenance period of 28 days, the sample's compressive strength rose to 2280 kPa. When the carbide slag level exceeds 8%, the presence of Ca(OH)2 in the mixture leads to the formation of carbonates, such as calcite, during the carbonization process. The organic matter subsequently undergoes a reaction with the Ca(OH)2 produced during the hydration of the mixture, leading to the formation of a highly soluble complex. As a result, only a limited quantity of calcium ions in the pore solution participate in the pozzolanic reaction, hence reducing the formation of gel reaction products such C-S-H.

The rapid development of modern communication systems propels the demand of high performance and miniaturization of RF/microwave components. Power dividers and filters are indispensable circuit components in amplifiers and antenna feed-networks which occupy large footprint of circuit broads, particularly at low frequency. The purpose of this study is to initiate a new type of planar transmission line with unique dual signal-path characteristics and ultimately achieve circuit miniaturization. In this paper, a novel technique of consolidating microstrip lines (MLs) and coplanar waveguide (CPW) lines on a single dielectric substrate, designated as the composite microstrip/CPW line (CM/CPW line), is proposed. With reference to the average line length of ML and CPW, it warrants a physical length reduction of 48.3% and further achieves a reduction of 80.8% after zigzagging CM/CPW line. The proof-of-concept design example of a two-way Wilkinson power divider (WPD) has demonstrated the effectiveness of the miniaturization technique. The simulation, in combination with the experimental results, validates that a size reduction factor of 86.9% when compared to conventional one has been achieved while maintaining a fractional bandwidth of 57.7% for the WPD.

Background: Stroke is a major health concern and a leading cause of mortality and morbidity. We and other groups have documented that hyperbaric oxygen preconditioning could significantly alleviate neuronal damage in ischemia‒reperfusion models through various mechanisms. However, we found that some of the subjects did not benefit from preconditioning with hyperbaric oxygen. The preconditioning phenomenon is similar to vaccination, in which the endogenous survival system is activated to fight against further injuries. However, with vaccine inoculations, we could test for specific antibodies against the pathogens to determine if the vaccination was successful. Likewise, this experiment was carried out to explore a biomarker that can reveal the effectiveness of the preconditioning before neuronal injury occurs. Methods: Middle cerebral artery occlusion (MCAO) was used to induce focal cerebral ischemia-reperfusion injury. 2D-DIGE-MALDI-TOF-MS/MS proteomic technique was employed to screen the differentially expressed proteins in the serum of rats among the control (Con) group (MCAO model without hyperbaric oxygen (HBO) preconditioning), hyperbaric oxygen protective (HBOP) group (in which the infarct volume decreased after HBO preconditioning vs. Con), and hyperbaric oxygen nonprotective (HBOU) group (in which the infarct volume remained the same or even larger after HBO preconditioning vs. Con). Candidate biomarkers were confirmed by western blot and enzyme linked immunosorbent assay (ELISA), and the relationship between the biomarkers and the prognosis of cerebral injury was further validated. Results: Among the 15 differentially expressed protein spots detected in the HBOP group by Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE), 3 spots corresponding to 3 different proteins (haptoglobin, serum albumin, and haemopexin) products were identified by MALDI-TOF-MS/MS. Serum albumin and haemopexin were upregulated, and haptoglobin was downregulated in the HBOP group (p < 0.05 vs. Con and HBOU groups). After the western blot study, only the changes in haemopexin were validated and exhibited similar changes in subjects from the HBOP group in accordance with MALDI-TOF-MS/MS proteomic analysis and enzyme linked immunosorbent assay (ELISA) analysis. The serum level of the hemopexin (HPX) at 2 h after HBO preconditioning was correlated with the infarct volume ratio after MCAO. Conclusions: Haemopexin may be developed as a predictive biomarker that indicated the effectiveness of a preconditioning strategy against cerebral ischaemic injury.

Schizophrenia is a severe mental disorder that is often comorbid with heart dysfunction and even sudden cardiac death (SCD). Clinical studies of SCD in schizophrenia have been largely reported, while there are limited autopsy studies that directly showed whole-scale information of such events. In this study, we present nine autopsy-based SCD cases in schizophrenia patients who died suddenly during hospitalization. Their medical records before and during hospitalization, and postmortem autopsy findings were summarized. These decedents had an average duration of schizophrenia for 6.83 ± 3.75 years with a male/female ratio of 4:5. They were all on intermittent antipsychotics medication before hospitalization and died within 15 days after hospitalization. Seven of the nine cases (77.8%) died of organic heart diseases such as severe coronary artery atherosclerosis (n = 4), myocarditis (n = 1), cardiomyopathy (n = 1), and pulmonary thromboembolism (n = 1). Two cases remained unexplained after systemic autopsy and toxicological examinations. Postmortem autopsy identified hepatic steatosis (n = 6) and respiratory inflammation (n = 3) as the most common associate extra-cardiac lesions. Our data provided autopsy-based data of SCD cases in schizophrenia and highlighted an intensive care of such patients during hospitalization.

In this manuscript, we reconsider the amplitude and phase combination of the plane-wave generator (PWG) from an electromagnetic field inverse radiation perspective and further propose a new method based on the Gauss-Newton inversion (GNI) to perform the inverse design of PWG. Furthermore, the wideband antenna pattern is applied in the transfer function and makes the inversion results closer to the engineering implementation. Compared with the traditional least-squares method (LSM) and singular value decomposition (SVD) method, the proposed method owns the merits of less amplitude range of the input source and stronger robustness.