Search

Your search keyword '"Shinohara, Tokuyuki"' showing total 4 results
Start Over Author "Shinohara, Tokuyuki" Publication Year Range Last 3 years
4 results on '"Shinohara, Tokuyuki"'

1. Human iPSC-derived CD4+ Treg-like cells engineered with chimeric antigen receptors control GvHD in a xenograft model

Author

Yano, Hisashi, primary, Koga, Keiko, additional, Sato, Takayuki, additional, Shinohara, Tokuyuki, additional, Iriguchi, Shoichi, additional, Matsuda, Atsushi, additional, Nakazono, Kazuki, additional, Shioiri, Maki, additional, Miyake, Yasuyuki, additional, Kassai, Yoshiaki, additional, Kiyoi, Hitoshi, additional, and Kaneko, Shin, additional

Published
2024
Full Text
View/download PDF

4. Human iPSC-derived CD4+Treg-like cells engineered with chimeric antigen receptors control GvHD in a xenograft model

Author

Yano, Hisashi, Koga, Keiko, Sato, Takayuki, Shinohara, Tokuyuki, Iriguchi, Shoichi, Matsuda, Atsushi, Nakazono, Kazuki, Shioiri, Maki, Miyake, Yasuyuki, Kassai, Yoshiaki, Kiyoi, Hitoshi, and Kaneko, Shin

Abstract

CD4+T cells induced from human iPSCs (iCD4+T cells) offer a therapeutic opportunity for overcoming immune pathologies arising from hematopoietic stem cell transplantation. However, most iCD4+T cells are conventional helper T cells, which secrete inflammatory cytokines. We induced high-level expression of FOXP3, a master transcription factor of regulatory T cells, in iCD4+T cells. Human iPSC-derived, FOXP3-induced CD4+T (iCD4+Treg-like) cells did not secrete inflammatory cytokines upon activation. Moreover, they showed demethylation of the Treg-specific demethylation region, suggesting successful conversion to immunosuppressive iCD4+Treg-like cells. We further assessed these iCD4+Treg-like cells for CAR-mediated immunosuppressive ability. HLA-A2 CAR-transduced iCD4+Treg-like cells inhibited CD8+cytotoxic T cell (CTL) division in a mixed lymphocyte reaction assay with A2+allogeneic CTLs and suppressed xenogeneic graft-versus-host disease (GVHD) in NSG mice treated with A2+human PBMCs. In most cases, these cells suppressed the xenogeneic GvHD progression as much as natural CD25+CD127−Tregs did.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results