74 results on '"Shinoda, Y."'
Search Results
2. Brainstem neural circuits triggering vertical saccades and fixation
- Author
-
Takahashi, M., primary, Sugiuchi, Y., additional, and Shinoda, Y., additional
- Published
- 2023
- Full Text
- View/download PDF
3. Brainstem Neural Circuits Triggering Vertical Saccades and Fixation.
- Author
-
Takahashi, M., Sugiuchi, Y., and Shinoda, Y.
- Abstract
Neurons in the nucleus raphe interpositus have tonic activity that suppresses saccadic burst neurons (BNs) during eye fixations, and that is inhibited before and during saccades in all directions (omnipause neurons, OPNs). We have previously demonstrated via intracellular recording and anatomical staining in anesthetized cats of both sexes that OPNs are inhibited by BNs in the medullary reticular formation (horizontal inhibitory BNs, IBNs). These horizontal IBNs receive monosynaptic input from the caudal horizontal saccade area of the superior colliculus (SC), and then produce monosynaptic inhibition in OPNs, providing a mechanism to trigger saccades. However, it is well known that the neural circuits driving horizontal components of saccades are independent from the circuits driving vertical components. Thus, our previous results are unable to explain how purely vertical saccades are triggered. Here, we again apply intracellular recording to show that a disynaptic vertical IBN circuit exists, analogous to the horizontal circuit. Specifically, we show that stimulation of the SC rostral vertical saccade area produces disynaptic inhibition in OPNs, which is not abolished by midline section between the horizontal IBNs. This excludes the possibility that horizontal IBNs could be responsible for the OPN inhibition during vertical saccades. We then show that vertical IBNs in the interstitial nucleus of Cajal, which receive monosynaptic input from rostral SC, are responsible for the disynaptic inhibition of OPNs. These results indicate that a similarly functioning SC-IBN-OPN circuit exists for both the horizontal and vertical oculomotor pathways. These two IBN-mediated circuits are capable of triggering saccades in any direction. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. The role of non-English-language science in informing national biodiversity assessments
- Author
-
Amano, T., Berdejo-Espinola, V., Akasaka, M., de Andrade Junior, M.A.U., Blaise, N., Checco, J., Çilingir, F.G., Citegetse, G., Tor, M.C., Drobniak, S.M., Giakoumi, S., Golivets, Maryna, Ion, M.C., Jara-Díaz, J.P., Katayose, R., Lasmana, F.P.S., Lin, H.-Y., Lopez, E., Mikula, P., Morales-Barquero, L., Mupepele, A.-C., Narváez-Gómez, J.P., Nguyen, T.H., Nogueira Lisboa, S., Nuñez, M.A., Pavón-Jordán, D., Pottier, P., Prescott, G.W., Samad, F., Šćiban, M., Seo, H.-M., Shinoda, Y., Vajna, F., Vozykova, S., Walsh, J.C., Wee, A.K.S., Xiao, H., Zamora-Gutierrez, V., Amano, T., Berdejo-Espinola, V., Akasaka, M., de Andrade Junior, M.A.U., Blaise, N., Checco, J., Çilingir, F.G., Citegetse, G., Tor, M.C., Drobniak, S.M., Giakoumi, S., Golivets, Maryna, Ion, M.C., Jara-Díaz, J.P., Katayose, R., Lasmana, F.P.S., Lin, H.-Y., Lopez, E., Mikula, P., Morales-Barquero, L., Mupepele, A.-C., Narváez-Gómez, J.P., Nguyen, T.H., Nogueira Lisboa, S., Nuñez, M.A., Pavón-Jordán, D., Pottier, P., Prescott, G.W., Samad, F., Šćiban, M., Seo, H.-M., Shinoda, Y., Vajna, F., Vozykova, S., Walsh, J.C., Wee, A.K.S., Xiao, H., and Zamora-Gutierrez, V.
- Abstract
Consulting the best available evidence is key to successful conservation decision-making. While much scientific evidence on conservation continues to be published in non-English languages, a poor understanding of how non-English-language science contributes to conservation decision-making is causing global assessments and studies to practically ignore non-English-language literature. By investigating the use of scientific literature in biodiversity assessment reports across 37 countries/territories, we have uncovered the established role of non-English-language literature as a major source of information locally. On average, non-English-language literature constituted 65% of the references cited, and these were recognized as relevant knowledge sources by 75% of report authors. This means that by ignoring non-English-language science, international assessments may overlook important information on local and/or regional biodiversity. Furthermore, a quarter of the authors acknowledged the struggles of understanding English-language literature. This points to the need to aid the use of English-language literature in domestic decision-making, for example, by providing non-English-language abstracts or improving and/or implementing machine translation. (This abstract is also avaialble in 21 other languages in Supplementary Data 4).
- Published
- 2023
5. Entrepreneurship and Prospects as a Concrete Engineer
- Author
-
Shinoda, Y., primary
- Published
- 2022
- Full Text
- View/download PDF
6. Impact of low-dose or under-dose direct oral anticoagulant on coagulation and fibrinolytic markers in patients with atrial fibrillation
- Author
-
Watanabe, T, primary, Tachibana, K, additional, Shinoda, Y, additional, Minamisaka, T, additional, Inui, H, additional, Ueno, K, additional, Inoue, S, additional, and Hoshida, S, additional
- Published
- 2021
- Full Text
- View/download PDF
7. Massive calcification around large joints in a patient subsequently diagnosed with adult-onset hypophosphatasia
- Author
-
Koga, M., primary, Kinoshita, Y., additional, Kato, H., additional, Kobayashi, H., additional, Shinoda, Y., additional, Nangaku, M., additional, Makita, N., additional, Dahir, K. M., additional, and Ito, N., additional
- Published
- 2021
- Full Text
- View/download PDF
8. Adequate Vegetable Intake Improves Metabolic Indices in Healthy Japanese Participants: A Randomized Crossover Study.
- Author
-
Kawakami-Shinoda Y, Sato M, Bao A, Zheng X, Kamiya M, Li G, Hosaka T, Goda T, and Arai H
- Abstract
Aim: We aimed to elucidate the effect of a healthy diet containing adequate amounts of protein and vegetables on metabolic indices., Methods: In this randomized crossover study, twenty-two healthy Japanese participants ingested two different test meals: fish diet (F) or fish diet with adequate vegetable content (FV). Each 5-day diet load test was separated by a washout period of at least seven days. Metabolic indices were measured in fasting blood and 24-h urine samples., Results: The delta (Δ) plasma glucose and Δserum low-density lipoprotein (LDL) cholesterol concentrations were significantly larger in the participants in group FV than in group F (p=0.042, p=0.013, respectively). The urinary pH in participants in group F on day 6 was significantly lower than on day 1 (p=0.008), and the Δurinary pH and Δnet gastrointestinal absorption of alkali of participants in group FV tended to be smaller than in group F (p=0.070, p=0.075, respectively)., Conclusions: This study showed that a healthy diet containing adequate protein and vegetables reduced the dietary acid load and improved plasma glucose and serum LDL concentrations in healthy Japanese participants.
- Published
- 2024
- Full Text
- View/download PDF
9. Factors associated with work sustainability in patients with bone metastasis.
- Author
-
Sawada R, Shinoda Y, Ohki T, Ishibashi Y, Kobayashi H, Tanaka S, and Haga N
- Subjects
- Humans, Female, Male, Middle Aged, Surveys and Questionnaires, Aged, Adult, Bone Neoplasms secondary, Quality of Life, Employment statistics & numerical data
- Abstract
Background: Working while receiving cancer treatment is challenging for patients, with considerable impact on their quality of life (QOL). However, there have been no reports on the factors that prevent employment in patients with bone metastases. This study aimed to investigate the employment status and factors impacting the continued employment of patients with bone metastases., Methods: We analyzed clinical data from new patients consulting The University of Tokyo Hospital team for bone metastasis treatment between June 2015 and September 2017. Patients who were working at the time of cancer diagnosis (n = 124) completed four QOL questionnaires. Factors associated with work sustainability were identified via univariate analysis and a chi-squared test. Multivariate logistic regression analysis was used for significant variables. Relationships between employment and QOL scales were investigated using the Wilcoxon rank-sum test, with P < .05 considered as statistically significant., Results: Among the 124 patients, only 45 (36.3%) were still working when the questionnaire was administered. Multivariate analysis revealed temporary employment, lytic or mixed bone metastases, and lower limb or acetabular metastasis, as significant factors hindering work sustainability. The QOL scores were high in the continued employment group. However, the relationship between employment status and pain remains unclear., Conclusions: Lytic or mixed bone metastases and the lower limb and acetabular metastasis were significantly associated with employment resignation. Mobility difficulties may prevent patients with bone metastases from sustaining employment. Collaboration between rehabilitation professionals, oncologists, and workplaces is imperative to address this problem., (© The Author(s) 2024. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2024
- Full Text
- View/download PDF
10. Rate-Dependent Pacemap Matching in Scar-Related Ventricular Tachycardia: Impact of "TR Fusion" Phenomenon.
- Author
-
Shinoda Y, Jameria ZA, Sahara N, Upadhyay GA, Liao Y, Martinez J, Katrapati P, Bai R, Zawaneh M, Weiss JP, Su W, and Tung R
- Abstract
Background: The impact of varying rates of pacemapping (PM) rates on QRS morphology and PM score matching in patients with scar-related ventricular tachycardia (VT) has not been systematically assessed., Objectives: In this study, we sought to assess the variability in PM score matching at different pacing rates., Methods: During substrate mapping for VT ablation, PM was performed at cycle lengths (CLs) of 600 ms, 500 ms, 400 ms, 300 ms, and VT CL. PM scores were compared for the entire QRS, the first half (H1) of QRS, and the second half (H2) of QRS to examine the influence of the preceding T wave superimposed into the onset of paced QRS complex (TR fusion)., Results: A total of 269 PMs in 40 patients undergoing scar-related VT ablation were systematically analyzed. The PM score improved at rates closer to VT with a median difference of 6% (Q1-Q3: 4%-10%; range: 0-33%) between the lowest and the highest PM scores at a given site. Greater slurring of the QRS onset was observed at faster-paced CL, corresponding to a superimposition of the preceding T wave into QRS onset, with significant differences in H1 but not H2 of the QRS complex. At faster PM rates, 32% of overall sites developed pseudo delta wave and 69% of endocardial pacing sites fulfilled epicardial criteria., Conclusions: The rate of pacemapping can significantly alter morphologic score matching, with the most optimal match observed closest to VT CL. The onset of QRS complex morphology is influenced by superimposition of the preceding T wave at faster rates, resulting in an underrecognized TR fusion phenomenon that may confound epicardial electrocardiographic criteria predicated upon the initial QRS slope and vector., Competing Interests: Funding Support and Author Disclosures Dr Tung has received speaking honoraria from Abbott, Medtronic, Boston Scientific, and Biotronik. The other authors have reported that they have no relationships relevant to the contents of this paper to disclose., (Copyright © 2024 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
11. Enzyme-Free In-Situ Electrochemical Measurement Using a Porous Membrane Electrode for Glucose Transport into Cell Spheroids.
- Author
-
Utagawa Y, Ino K, Shinoda Y, Yamazaki M, Abe H, and Shiku H
- Subjects
- Humans, Porosity, MCF-7 Cells, Biological Transport, Glucose metabolism, Glucose analysis, Spheroids, Cellular metabolism, Electrodes, Electrochemical Techniques methods, Electrochemical Techniques instrumentation
- Abstract
Microphysiological systems have attracted attention because of their use in drug screening. However, it is challenging to measure cell functions in real time using a device. In this study, we developed a cell culture device using a porous membrane electrode for in situ electrochemical glucose measurements for cell analysis. First, a porous membrane electrode was fabricated and electrochemically evaluated for enzyme-free glucose measurement. Subsequently, the glucose uptake of MCF-7 spheroids was evaluated using living spheroids, fixed spheroids, supernatants, and glucose transporter inhibitor-treated spheroids. Conventionally, the direct optical measurement of glucose uptake requires fluorescence-labeled glucose derivatives. In addition, the glucose uptake can be evaluated by measuring the glucose concentration in the medium by optical or electrochemical measurements. However, glucose needs to be consumed in the entire cell culture medium, which needs a long culture time. In contrast, our system can measure glucose in approximately 5 min without any labels because of in situ electrochemical measurements. This system can be used for in situ measurements in in vitro cell culture systems, including organ-on-a-chip for drug screening.
- Published
- 2024
- Full Text
- View/download PDF
12. Optimal cardiac rhythm during substrate mapping in scar-related ventricular tachycardia: Significance of wavefront direction on identifying critical sites.
- Author
-
Shinoda Y, Komatsu Y, Hattori M, Oda Y, Iioka Y, Hanaki Y, Yamasaki H, Igarashi M, Ishizu T, and Nogami A
- Subjects
- Humans, Male, Female, Retrospective Studies, Aged, Heart Conduction System physiopathology, Middle Aged, Body Surface Potential Mapping methods, Heart Rate physiology, Heart Ventricles physiopathology, Electrophysiologic Techniques, Cardiac methods, Tachycardia, Ventricular physiopathology, Tachycardia, Ventricular etiology, Tachycardia, Ventricular diagnosis, Cicatrix physiopathology, Cicatrix diagnosis, Cicatrix etiology, Catheter Ablation methods
- Abstract
Background: A rotational activation pattern (RAP) around the localized line of a conduction block often correlates with sites specific to the critical zones of ventricular tachycardia (VT). The wavefront direction during substrate mapping affects manifestation of the RAP and line of block., Objective: The purpose of this study was to investigate the most optimal cardiac rhythm for identifying RAP and line of block in substrate mapping., Methods: We retrospectively evaluated 71 maps (median 3205 points/map) in 46 patients (65 ± 15 years; 33% with ischemic cardiomyopathy) who underwent high-density substrate mapping and ablation of scar-related VT. Appearance of a RAP during sinus, right ventricular (RV)-paced, left ventricular (LV)-paced, and biventricular-paced rhythms was investigated., Results: RAP was identified in 24 of 71 maps (34%) in the region where wavefronts from a single direction reached but not in the region where wavefronts from multiple directions centripetally collided. The probability of identifying the RAP depended on scar location; that is, anteroseptal and inferoseptal, inferior and apical, and basal lateral RAPs were likely to be identified during sinus/atrial, RV-paced, and LV-paced rhythms, respectively. In 13 patients, the RAP was not evident in the baseline map but became apparent during remapping in the other rhythm, in which the wavefront reached the site earlier within the entire activation time., Conclusion: The optimal rhythm for substrate mapping depends on the spatial distribution of the area of interest. A paced rhythm with pacing sites near the scar may facilitate the identification of critical VT zones., Competing Interests: Disclosures Dr Komatsu has received honoraria for lectures and advisory board activities from Johnson & Johnson. Dr Yamasaki received a scholarship fund from Johnson & Johnson. Dr. Igarashi received an endowment from Japan Lifeline, Astec Co. Ltd., Medtronic, DVX, Boston Scientific, Abbott Medical Inc., and Biotronik. Dr Nogami received honoraria from Abbott and Johnson & Johnson; and endowments from Medtronic and DVX. All other authors have no conflicts of interest to disclose., (Copyright © 2024 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
13. Calcium-dependent activator protein for secretion 2 is involved in dopamine release in mouse midbrain neurons.
- Author
-
Iguchi H, Katsuzawa T, Saruta C, Sadakata T, Kobayashi S, Sato Y, Sato A, Sano Y, Maezawa S, Shinoda Y, and Furuichi T
- Abstract
The Ca
2+ -dependent activator protein for secretion (CAPS/CADPS) family protein facilitates catecholamine release through the dense-core vesicle exocytosis in model neuroendocrine cell lines. However, it remains unclear if it induces dopamine release in the central neurons. This study aimed to examine the expression and function of CADPS2, one of the two CADPS paralogs, in dopamine neurons of the mouse midbrain. This study shows that CADPS2 was expressed in tyrosine hydroxylase and the vesicular monoamine transporter 2 (VMAT2)-positive dopaminergic neurons of the midbrain samples and primary mesencephalic cell cultures. Subcellular fractions rich in dopamine were collected using immunoaffinity for CADPS2 from midbrain protein extracts. Cell imaging using fluorescent false neurotransmitter FFN511 as a substrate for VMAT2 showed decreased activity-dependent dopamine release in Cadps2 -deficient cultures, compared to that in wild-type cultures. These results suggest that CADPS2 is involved in dopamine release from the central neurons, indicating its involvement in the central dopamine pathway., Competing Interests: The authors declare that the research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Iguchi, Katsuzawa, Saruta, Sadakata, Kobayashi, Sato, Sato, Sano, Maezawa, Shinoda and Furuichi.)- Published
- 2024
- Full Text
- View/download PDF
14. Clinical outcomes of spinal cord stimulation in patients with intractable leg pain in Japan.
- Author
-
Ueno K, Tachibana K, Masunaga N, Shinoda Y, Minamisaka T, Inui H, Amiya R, Inoue S, Murakami A, and Hoshida S
- Subjects
- Humans, Female, Male, Aged, Retrospective Studies, Japan, Treatment Outcome, Aged, 80 and over, Middle Aged, Pain Management methods, Pain Measurement methods, Spinal Cord Stimulation methods, Leg, Pain, Intractable therapy
- Abstract
Background: Neuromodulation through spinal cord stimulation (SCS) is a therapeutic option for relieving leg pain and improving the chances of limb salvage in patients with intractable chronic limb-threatening ischemia (CLTI); however, there is no consensus on its indications., Objective: The aim of this study was to assess the clinical outcomes of SCS in patients with intractable leg pain caused by various diseases treated in the department of cardiovascular medicine in Japan., Methods: This was a retrospective study of patients who underwent SCS for pain management. Patients were considered eligible for the therapy if they met the following criteria: (1) intractable leg pain (numerical rating scale [NRS] score of 10), (2) no revascularization option, and (3) no septicemia., Results: Twenty patients (mean age: 77 years; men/women: 11/9) were included in this study. The NRS score of the patients significantly reduced from 10 ± 0 before procedure to 4 ± 3 at discharge (p < 0.001). The clinical response rate of the entire cohort was 65% (13/20) at 17 ± 14 months after implantation; however, patients with intractable CLTI showed a low response rate (45%), whereas those with subacute limb ischemia showed a high response rate (100%). A multivariable regression analysis showed that hemoglobin level was significantly associated with treatment response, even after adjusting for age and sex (p = 0.026). The area under the receiver operating characteristic curve for the correlation between hemoglobin level (cutoff, 11.4 g/dL) and clinical response to SCS was 0.824 (0.619-1)., Conclusions: SCS can reduce clinical symptoms in majority of patients with intractable leg pain. Although implantation of an SCS device has been shown to improve microvascular perfusion insufficiency, the correlation between hemoglobin level and the clinical effect of SCS indicates that a preserved microcirculatory vascular bed is essential for the therapy to be effective., (© 2024 World Institute of Pain.)
- Published
- 2024
- Full Text
- View/download PDF
15. Clinical characteristics and survival outcomes of elderly patients with de novo metastatic germ cell tumors.
- Author
-
Kirisawa T, Okuno T, Hagimoto H, Matsuda A, Maejima A, Shinoda Y, Nakamura E, Komiyama M, Fujimoto H, and Matsui Y
- Subjects
- Humans, Male, Middle Aged, Adult, Age Factors, Retrospective Studies, Aged, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Disease-Free Survival, Induction Chemotherapy methods, Neoplasm Metastasis, Prognosis, Young Adult, Neoplasms, Germ Cell and Embryonal mortality, Neoplasms, Germ Cell and Embryonal secondary, Neoplasms, Germ Cell and Embryonal drug therapy, Neoplasms, Germ Cell and Embryonal therapy, Neoplasms, Germ Cell and Embryonal pathology, Testicular Neoplasms mortality, Testicular Neoplasms pathology, Testicular Neoplasms drug therapy, Testicular Neoplasms therapy
- Abstract
Objectives: To determine the outcomes for elderly patients with de novo metastatic germ cell tumors and the influence of patient age on adherence to standard chemotherapy., Methods: A total of 150 patients who were initially diagnosed with metastatic germ cell tumors and treated at our institution between 2007 and 2021 were included. Patients were classified according to three age groups: aged <40, 40-49, and ≥50 years. Clinicopathological features, adherence to standard first-line chemotherapy, overall survival, and disease-free survival were compared between these groups. We also analyzed the outcomes of patients who received low-intensity induction chemotherapy due to adverse events and/or comorbidities., Results: There was no significant difference in any of the survival outcomes and in the rate of adherence to standard first-line chemotherapy between the three age groups, although elderly patients with intermediate/poor prognosis group tended to receive less-intense chemotherapies. The rate of febrile neutropenia as a chemotherapy-related adverse event was significantly higher in patients aged ≥50 years. No statistical significance in survival outcomes was detected between the group of patients who received relatively low-intensity induction chemotherapy and those who received adequately intensive planned chemotherapy., Conclusions: The adherence rate of standard fist-line chemotherapy of elderly patients is almost comparable to that of younger patients, although some adverse events should be carefully managed. Even elderly patients with metastatic germ cell tumors can aim for equivalently good survival outcome like younger populations, with effort to adhere to standard chemotherapy., (© 2024 The Japanese Urological Association.)
- Published
- 2024
- Full Text
- View/download PDF
16. Evaluation of long-term pharmacist-led prospective audit and feedback in antimicrobial stewardship: An 8-year study.
- Author
-
Ohashi K, Matsuoka T, Shinoda Y, Takahashi T, Shikano H, Kagajo M, Yagi T, and Usami E
- Subjects
- Humans, Prospective Studies, Japan, Feedback, Female, Male, Pseudomonas aeruginosa drug effects, Antimicrobial Stewardship methods, Pharmacists, Anti-Bacterial Agents therapeutic use
- Abstract
Background: Limited research has evaluated the long-term outcomes of prospective audit and feedback (PAF) led by pharmacists. We assessed pharmacist-led PAF processes and outcome measures over 8 years., Methods: This study was conducted at a single public hospital in Japan. Between 2014 and 2021, pharmacists conducted weekday PAFs for hospitalized patients and annually evaluated the process and outcome measures. The endpoints included detection of drug-resistant bacteria, drug susceptibility rates, duration of antimicrobial therapy, and proportion of long-term administration., Results: Among inpatients, methicillin-resistant Staphylococcus aureus significantly decreased from 50.9% in 2014 to 32.8% in 2021 (P < .001). The susceptibility rate of Pseudomonas aeruginosa to meropenem significantly increased from 91.2% in 2014 to 94.4% in 2021 (P < .001) and levofloxacin increased from 84.9% in 2014 to 89.3% in 2021 (P < .001). Antimicrobial therapy duration did not differ significantly between 2014 and 2016, but decreased significantly from 2017 onwards (P < .001), except in 2018. The number of patients receiving intravenous antimicrobials for >10 days decreased significantly between 2014 and 2021 (P < .001)., Conclusions: Long-term continuous PAF interventions by pharmacists contribute to improving and maintaining process and outcome indicators and prevent the spread of drug-resistant bacteria., (Copyright © 2024 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
17. AlveoMPU: Bridging the Gap in Lung Model Interactions Using a Novel Alveolar Bilayer Film.
- Author
-
Hirano M, Iwata K, Yamada Y, Shinoda Y, Yamazaki M, Hino S, Ikeda A, Shimizu A, Otsuka S, Nakagawa H, and Watanabe Y
- Abstract
The alveoli, critical sites for gas exchange in the lungs, comprise alveolar epithelial cells and pulmonary capillary endothelial cells. Traditional experimental models rely on porous polyethylene terephthalate or polycarbonate membranes, which restrict direct cell-to-cell contact. To address this limitation, we developed AlveoMPU, a new foam-based mortar-like polyurethane-formed alveolar model that facilitates direct cell-cell interactions. AlveoMPU features a unique anisotropic mortar-shaped configuration with larger pores at the top and smaller pores at the bottom, allowing the alveolar epithelial cells to gradually extend toward the bottom. The underside of the film is remarkably thin, enabling seeded pulmonary microvascular endothelial cells to interact with alveolar epithelial cells. Using AlveoMPU, it is possible to construct a bilayer structure mimicking the alveoli, potentially serving as a model that accurately simulates the actual alveoli. This innovative model can be utilized as a drug-screening tool for measuring transepithelial electrical resistance, assessing substance permeability, observing cytokine secretion during inflammation, and evaluating drug efficacy and pharmacokinetics.
- Published
- 2024
- Full Text
- View/download PDF
18. Successful ablation of premature ventricular contractions from "late" activation site in cardiac sarcoidosis: Evidence suggestive of a reentrant mechanism.
- Author
-
Shinoda Y, Shatz NA, and Tung R
- Subjects
- Humans, Electrocardiography, Male, Middle Aged, Female, Heart Conduction System physiopathology, Sarcoidosis physiopathology, Sarcoidosis surgery, Sarcoidosis diagnosis, Sarcoidosis complications, Ventricular Premature Complexes physiopathology, Ventricular Premature Complexes surgery, Ventricular Premature Complexes diagnosis, Cardiomyopathies physiopathology, Cardiomyopathies diagnosis, Cardiomyopathies surgery, Catheter Ablation methods
- Abstract
Competing Interests: Disclosures Dr Tung has received speaking honoraria from Abbott, Boston Scientific, and Biosense Webster. All other authors have no conflicts of interest to disclose.
- Published
- 2024
- Full Text
- View/download PDF
19. Vancomycin-Induced Neutropenia With Subsequent Perioperative Readministration: A Case Report.
- Author
-
Shinoda Y, Kawabata T, Ohashi K, and Usami E
- Abstract
Vancomycin (VCM), an essential antibiotic for antimicrobial-resistant Gram-positive cocci, can lead to complications such as neutropenia. Here, we present a case of a 25-year-old male with noncommunicating hydrocephalus due to an intraventricular tumor who developed neutropenia during VCM therapy. Despite the suspected VCM-induced neutropenia, short-term readministration was deemed necessary for perioperative infection prophylaxis. This patient was readministered without neutropenia. A review of the literature revealed an earlier onset of VCM-induced neutropenia than that previously reported, emphasizing the importance of vigilant monitoring. Although readministration of VCM in patients with neutropenia is uncommon, it may be feasible with careful risk assessment, particularly in cases of mild neutropenia and short-term therapy. However, the mechanisms underlying VCM-induced neutropenia remain unclear, necessitating further research on the optimal management strategies., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2024, Shinoda et al.)
- Published
- 2024
- Full Text
- View/download PDF
20. Incidence of cryoballoon expansion dislodgement during pulmonary vein isolation-An underappreciated frequent cause of incomplete isolation.
- Author
-
Liao Y, Tomaiko-Clark ED, Martinez J, Shinoda Y, Morris MF, Liu Z, Shatz DY, Katrapati P, Sahara N, Weiss JP, Zawaneh MS, Tung R, Bai R, and Su W
- Subjects
- Humans, Incidence, Treatment Outcome, Pulmonary Veins surgery, Atrial Fibrillation surgery, Cryosurgery methods, Catheter Ablation methods
- Abstract
Background: Cryoballoon ablation for atrial fibrillation (AF) requires adequate contact between the pulmonary vein (PV) antrum and cryoballoon. The surge of intraballoon pressure during the initial phase of ablation may change the balloon's shape and compliance, resulting in balloon dislodgement and loss of PV occlusion. Without continuous monitoring, this phenomenon is often undetected but can be associated with incomplete PV isolation (PVI)., Methods: Primary cryoablation of AF was performed in 15 patients. PV occlusion status pre- and post-freezing were analyzed with intracardiac echocardiography (ICE) and dielectric imaging-based occlusion tool (DIOT) to calculate the incidence of expansion dislodgement of cryoballoon., Results: A total of 105 cryoablation applications were performed on 57 veins, including three common ostiums of left pulmonary veins. In the evaluation of PV occlusion, both modalities reported consistent results in 86.7% of the assessments. Despite complete PV occlusion before ablation, peri-balloon leak after initiation of freezing was detected by ICE in 5/22 (22.7%) applications and by DIOT in 8/25 (32%) applications., Conclusion: Incidence of expansion dislodgement of the cryoballoon was detected in one-fourth to one-third of cryoablation applications depending on the imaging modality used, which was clinically frequent and significant., (© 2023 Wiley Periodicals LLC.)
- Published
- 2024
- Full Text
- View/download PDF
21. A case of acute purulent pericarditis due to MRSA treated with daily pericardial lavage for one month followed by pericardial fenestration.
- Author
-
Inoue S, Tachibana K, Masunaga N, Shinoda Y, Minamisaka T, Inui H, Ueno K, Amiya R, Murakami A, and Hoshida S
- Abstract
Acute purulent pericarditis is a rare infection in developed countries. We herein report a case with diabetic nephropathy under maintenance hemodialysis who suffered from acute purulent pericarditis caused by methicillin-resistant Staphylococcus aureus (MRSA). The treatment of purulent pericarditis mainly involves rapid administration of appropriate antibiotics and drainage. However, in this case, the patient was unresponsive to vancomycin and performing early surgical intervention was challenging due to highly pathogenic MRSA. Therefore, we performed pericardial fenestration in the chronic phase to suppress the risk of fatal secondary infections after daily irrigation for one month to reduce bacterial load mechanically., Learning Objective: In a case of purulent pericarditis caused by highly pathogenic methicillin-resistant Staphylococcus aureus resistant to antibiotics and resulting in constrictive pericarditis, it was possible to perform pericardial fenestration in the chronic phase, while mitigating the risk of fatal secondary infections, by controlling the inflammation through daily irrigation for a long time to reduce the bacterial load mechanically., Competing Interests: The authors declare that there is no conflict of interest., (© 2024 Japanese College of Cardiology. Published by Elsevier Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
22. End-of-life walking ability in cancer patients with spinal metastases.
- Author
-
Sawada R, Shinoda Y, Ohki T, Ishibashi Y, Kobayashi H, Matsubayashi Y, Tanaka S, and Haga N
- Subjects
- Humans, Spine, Walking, Death, Retrospective Studies, Spinal Neoplasms secondary, Spinal Cord Compression complications
- Abstract
Background: Even terminal cancer patients desire to walk to the toilet by themselves until the very last day. This study aimed to describe the walking ability of patients with spinal metastases at the end-of-life stage and identify the factors affecting this ability., Methods: Among 527 patients who first visited our multidisciplinary team for bone metastasis between 2013 and 2016, 56 patients who had spinal metastases with a Spinal Instability Neoplastic Score ≥7 and died during follow-up were included. We collected general clinical data, performance status, Frankel classification, epidural spinal cord compression scale and Spinal Instability Neoplastic Score at the first consultation. Patients' last day of walking and date of death were also examined. Univariate analyses (chi-squared tests) were performed to identify the factors that impacted walking ability 30 and 14 days before patients' death., Results: A total of 56 patients were extracted, and 57.1% (32/56) and 32.7% (16/49) of patients were ambulatory 30 and 14 days before death, respectively. Their performance status (P = 0.0007), Frankel grade (P = 0.012) and epidural spinal cord compression grade (P = 0.006) at the first examination, and administration of bone modifying agents during follow-up period (P = 0.029) were significantly related to walking ability 30 days before death. Among ambulatory patients 30 days before death, those with Spinal Instability Neoplastic Score ≥10 (P = 0.005), especially with high scores of collapse (P = 0.002) and alignment (P = 0.002), were less likely to walk 14 days before death. The walking period in the last month of their life was significantly longer in patients with total Spinal Instability Neoplastic Score 7-9 (P = 0.009) and in patients without collapse (P = 0.040) by the Wilcoxon test., Conclusion: The progression of spinal metastasis, especially neurological deficit, at the initial consultation were associated with walking ability 30 days before death, and spinal stability might be crucial for preserving walking ability during the last month. Early diagnosis and implementation of appropriate bone management might be important for the end-of-life walking ability., (© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2024
- Full Text
- View/download PDF
23. Risk Factors Causing Hypothyroidism in Patients With Head and Neck Cancer After Radiotherapy Using SIB-VMAT.
- Author
-
Yoshimura N, Fujiwara M, Igeta M, Suzuki H, Kunimoto R, Terada T, Shinoda Y, Nakamura M, Fukutake J, Takaki H, and Yamakado K
- Subjects
- Humans, Retrospective Studies, Risk Factors, Radiotherapy Dosage, Radiotherapy Planning, Computer-Assisted adverse effects, Radiotherapy, Intensity-Modulated adverse effects, Hypothyroidism epidemiology, Hypothyroidism etiology, Head and Neck Neoplasms complications
- Abstract
Background/aim: We evaluated the incidence of radiation-induced hypothyroidism and its risk factors in patients with head and neck cancer who underwent radiotherapy using simultaneous integrated boost-volumetric-modulated arc therapy (SIB-VMAT)., Patients and Methods: This retrospective study included 86 patients who received definitive radiotherapy using SIB-VMAT for head and neck cancer. The incidence of ≥ grade 2 hypothyroidism was evaluated. We also evaluated the relationships between hypothyroidism development and clinical factors and thyroid dose-volume parameters., Results: During a median follow-up period of 17 months (range=3-65 months), 31 patients (36.0%, 31/86) developed grade 2 hypothyroidism requiring hormone replacement therapy. No patients experienced ≥ grade 3 hypothyroidism. The cumulative incidences of hypothyroidism at 1 and 2 years after radiation therapy were 24.5% and 38.7%, respectively, with a median onset time of 10.0 months (range=3.0-35.0 months). Thyroid volume (p=0.003), volume of the thyroid spared at 60 Gy (VS60; cut-off value, 5.16 ml; p=0.009), VS70 (cut-off value, 8.0 ml; p=0.007), VS60 equivalent dose in 2 Gy fraction (EQD2; cut-off value, 7.78 ml; p=0.001), and VS70EQD2 (cut-off value, 10.59 ml; p=0.008) were significantly associated with the development of radiation-induced hypothyroidism., Conclusion: Radiation-induced hypothyroidism is not rare in patients with head and neck cancer undergoing radiotherapy using SIB-VMAT. Radiation dose-volume parameters detected in this study may be useful indicators to prevent this complication., (Copyright © 2024 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
24. Transcriptome analysis of cultured human vascular endothelial cells after γ-ray irradiation and correlation analysis with ATP, ADP, and adenosine as secondary soluble factors.
- Author
-
Fujie T, Kobayashi M, Ikeuchi L, Nakano T, Kitabatake K, Shinoda Y, Fujiwara Y, Yamamoto C, Tsukimoto M, and Kaji T
- Subjects
- Humans, Adenosine Triphosphate metabolism, Receptors, Purinergic, Gene Expression Profiling, Adenosine Diphosphate pharmacology, Cells, Cultured, Adenosine metabolism, Endothelial Cells metabolism
- Abstract
Vascular endothelial cells serve as barriers between blood components and subendothelial tissue and regulate the blood coagulation-fibrinolytic system. Ionizing radiation is a common physical stimulant that induces a bystander effect whereby irradiated cells influence neighboring cells through signalings, including purinergic receptor signaling, activated by adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP), and adenosine as secondary soluble factors. Human vascular endothelial EA.hy926 cells were cultured and irradiated with γ-rays or treated with ATP, ADP, or adenosine under non-toxic conditions. RNA-seq, gene ontology, and hierarchical clustering analyses were performed. The transcriptome analysis of differentially expressed genes in vascular endothelial cells after γ-ray irradiations suggests that the change of gene expression by γ-irradiation is mediated by ATP and ADP. In addition, the expression and activity of the proteins related to blood coagulation and fibrinolysis systems appear to be secondarily regulated by ATP and ADP in vascular endothelial cells after exposure to γ-irradiation. Although it is unclear whether the changes of the gene expression related to blood coagulation and fibrinolysis systems by γ-irradiation affected the increased hemorrhagic tendency through the exposure to γ-irradiation or the negative feedback to the activated blood coagulation system, the present data indicate that toxicity associated with γ-irradiation involves the dysfunction of vascular endothelial cells related to the blood coagulation-fibrinolytic system, which is mediated by the signalings, including purinergic receptor signaling, activated by ATP and ADP.
- Published
- 2024
- Full Text
- View/download PDF
25. Effect of harvesting strategy of second-cut orchardgrass silage on feed intake, digestion, and milk production in dairy cows.
- Author
-
Miyaji M, Yajima A, Yagi T, Shinoda Y, Komatsu M, Asakuma S, and Ueda Y
- Subjects
- Animals, Cattle physiology, Cattle metabolism, Female, Animal Nutritional Physiological Phenomena physiology, Dietary Fiber, Dairying methods, Time Factors, Silage, Lactation physiology, Digestion physiology, Eating physiology, Milk metabolism, Diet veterinary, Dactylis
- Abstract
We investigated the effects of regrowth interval and first-cut timing on the dietary characteristics of second-cut orchardgrass silage and feed intake and milk production in dairy cows fed second-cut orchardgrass silage. The second-cut grasses were harvested 7w after the first-cut at the early stage (E7w) or at the heading stage (H7w), or harvested 6w after the first-cut at the early stage (E6w) from orchardgrass sward, and then ensiled. We evaluated the effect of regrowth interval by comparing E7w and E6w, and the effect of first-cut timing by comparing E7w and H7w. Six multiparous Holstein cows were used in a replicated 3 × 3 Latin square design, with three dietary treatments: diets containing E7w, E6w, or H7w silage at 30% dietary dry matter. We observed that feeding E6w silage instead of E7w silage increased fiber digestibility, dry matter intake, and milk production; however, the first-cut timing (E7w vs. H7w) did not affect nutrient content and digestibility, feed intake, or lactation performance. These results show that harvesting at short regrowth intervals for second-cut orchardgrass can be an effective strategy for improving feed utilization and milk yield; however, the first-cut timing for second-cut orchardgrass has little impact., (© 2024 Japanese Society of Animal Science.)
- Published
- 2024
- Full Text
- View/download PDF
26. Comparative study of susceptibility to methylmercury cytotoxicity in cell types composing rat peripheral nerves: a higher susceptibility of dorsal root ganglion neurons.
- Author
-
Yoshida E, Aoki K, Sasaki Y, Izuhara H, Takahashi T, Fujiwara Y, Fujie T, Du K, Eto K, Shinoda Y, and Kaji T
- Subjects
- Animals, Cells, Cultured, Large Neutral Amino Acid-Transporter 1 metabolism, Large Neutral Amino Acid-Transporter 1 genetics, Multidrug Resistance-Associated Proteins metabolism, Multidrug Resistance-Associated Proteins genetics, Peripheral Nerves metabolism, Peripheral Nerves drug effects, Male, Rats, Multidrug Resistance-Associated Protein 2, Ganglia, Spinal metabolism, Ganglia, Spinal drug effects, Methylmercury Compounds toxicity, Schwann Cells drug effects, Schwann Cells metabolism, Cell Survival drug effects, ATP-Binding Cassette Transporters
- Abstract
Methylmercury is an environmental polluting organometallic compound that exhibits neurotoxicity, as observed in Minamata disease patients. Methylmercury damages peripheral nerves in Minamata patients, causing more damage to sensory nerves than motor nerves. Peripheral nerves are composed of three cell types: dorsal root ganglion (DRG) cells, anterior horn cells (AHCs), and Schwann cells. In this study, we compared cultured these three cell types derived from the rat for susceptibility to methylmercury cytotoxicity, intracellular accumulation of mercury, expression of L-type amino acid transporter 1 (LAT1), which transports methylmercury into cells, and expression of multidrug resistance-associated protein 2 (MRP2), which transports methylmercury-glutathione conjugates into the extracellular space. Of the cells examined, we found that DRG cells were the most susceptible to methylmercury with markedly higher intracellular accumulation of mercury. The constitutive level of LAT1 was higher and that of MRP2 lower in DRG cells compared with those in AHC and Schwann cells. Additionally, decreased cell viability caused by methylmercury was significantly reduced by either the LAT1 inhibitor, JPH203, or siRNA-mediated knockdown of LAT1. On the other hand, an MRP2 inhibitor, MK571, significantly intensified the decrease in the cell viability caused by methylmercury. Our results provide a cellular basis for sensory neve predominant injury in the peripheral nerves of Minamata disease patients.
- Published
- 2024
- Full Text
- View/download PDF
27. Significant reduction in burden of metastatic disease by intermittent docetaxel therapy in a patient with castration-resistant prostate cancer.
- Author
-
Kirisawa T, Nakamura E, Okuno T, Hagimoto H, Matsuda A, Shinoda Y, Komiyama M, Fujimoto H, and Matsui Y
- Abstract
Intermittent docetaxel therapy (IDT) is rarely used nowadays as a treatment option for men with metastatic castration-resistant prostate cancer (mCRPC) because of the widespread availability of androgen receptor axis-targeted therapy, which is less toxic. Therefore, there is limited information available on whether IDT has a clinical benefit in the treatment of men with mCRPC. This report describes the case of a 66-year-old man with a diagnosis of cT2N1M0 prostate cancer who underwent neoadjuvant combined androgen blockade and whole-pelvis radiation therapy. However, the tumor had progressed to mCRPC with metastasis to the bladder and a left pelvic lymph node within 2 years. Docetaxel had been administered as first-line chemotherapy, and the patient achieved a complete response in terms of the bladder metastasis. Docetaxel was stopped after 15 cycles. When a durable response had been maintained for more than 2 years, during which only androgen deprivation therapy was administered, the patient was switched to observation only. However, his prostate-specific antigen level gradually increased. Abiraterone was started as second-line therapy, during which there was a rapid increase in the PSA level. Computed tomography revealed further enlargement of the left pelvic lymph node, bladder metastasis, metastasis to the left common iliac lymph nodes, and several disseminated nodules around the bladder. Docetaxel was reintroduced as IDT for third-line therapy, and a complete response was achieved for all metastases, with the exception of the metastasis in the left pelvic lymph node. Thus far, the patient has survived for more than 7 years after starting docetaxel as first-line therapy for mCRPC. IDT is potentially useful in a subgroup of patients with mCRPC and could achieve long-term survival. Comprehensive genomic profiling may help physicians to select patients with mCRPC who are more likely to benefit from docetaxel than other systemic therapy., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interests., (© The Author(s) under exclusive licence to The Japan Society of Clinical Oncology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
- Published
- 2023
- Full Text
- View/download PDF
28. Balloon surface temperature-controlled ablation using a second-generation radiofrequency HotBalloon: an in vivo feasibility study.
- Author
-
Shinoda Y, Yamasaki H, Murakoshi N, Kohno T, Yamane T, Aonuma K, and Ishizu T
- Subjects
- Animals, Temperature, Vena Cava, Superior surgery, Feasibility Studies, Treatment Outcome, Pulmonary Veins surgery, Catheter Ablation methods, Atrial Fibrillation diagnosis, Atrial Fibrillation surgery, Atrial Fibrillation etiology
- Abstract
Aims: The first-generation radiofrequency HotBalloon (RHB) is a size-adjustable single-shot device used in atrial fibrillation. The energy output is determined by its central temperature and not by its balloon surface temperature (BST), thus limiting its efficacy and safety. Therefore, a second-generation RHB was developed to monitor BST and enable BST-controlled ablation. This animal study aims to evaluate the accuracy of a newly developed BST-monitoring system and validate the optimal BST for ablation., Methods and Results: In Protocol 1, thermocapsules were attached to the superior vena cava (SVC) epicardium. The accuracy of BST monitoring was examined during SVC isolation. In Protocol 2, the efficacy and safety of different BST-controlled ablations were examined. In the acute model, electrophysiological and pathological findings were assessed after energy applications with BST at 51, 54, 57, and 60°C. In the chronic model, the lesion durability and pathological findings were assessed 8 weeks after BST-controlled ablation (57 and 60°C). A significant positive correlation was found between the epicardial temperature and the BST-monitoring value (r = 0.98). In the acute model, all target veins were electrically isolated with BST-controlled ablation at ≥57°C (18/18, 100%). In the chronic model, durable lesions were observed in all veins at 60°C, while 44% of the veins showed reconnection at 57°C. In both pathological analyses, significantly greater lesions were observed at 60°C than at 57°C. There were no significant differences in adverse events between the two groups., Conclusion: Balloon surface temperature-controlled ablation at 60°C using the second-generation RHB may be optimal for creating durable lesions without compromising safety., Competing Interests: Conflict of interest: H.Y. received a consultant fee from Toray Industries, Inc. T.Y. received speaker honoraria from Medtronic Japan and BEG Co., Ltd and research grants from Japan Lifeline Co., Ltd. The other authors report no conflicts., (© The Author(s) 2023. Published by Oxford University Press on behalf of the European Society of Cardiology.)
- Published
- 2023
- Full Text
- View/download PDF
29. Novel function of transglutaminase 2 in extracellular histone-induced acute lung injury.
- Author
-
Mizuno T, Nagano F, Ito Y, Tatsukawa H, Shinoda Y, Takeuchi T, Takahashi K, Tsuboi N, Nagamatsu T, Yamada S, Maruyama S, and Hitomi K
- Subjects
- Animals, Mice, Mice, Inbred C57BL, Histones, Protein Glutamine gamma Glutamyltransferase 2, Fibrin, Endothelial Cells, Acute Lung Injury chemically induced
- Abstract
Extracellular histones induce endothelial damage, resulting in lung haemorrhage; however, the underlying mechanism remains unclear. Factor XIII, as a Ca
2+ -dependent cross-linking enzyme in blood, mediates fibrin deposition. As another isozyme, transglutaminase 2 (TG2) has a catalytic activity distributing in most tissues. Herein, we investigated whether TG2 promotes fibrin deposition and mediates the adhesion of platelets to ECs in histone-induced acute lung injury (ALI). We evaluated the lung histology and the adhesion of platelets to endothelial cells (ECs) after injecting histones to wild-type (WT) C57BL/6J and TG2 knockout (TG2-/- ) mice, and administered a TG2 inhibitor (NC9) to WT mice. Pulmonary haemorrhage was more severe in TG2-/- mice than that in WT mice. The area of fibrin deposition and the proportion of CD41+ CD31+ cells were lower in TG2-/- mice than in WT mice. Pre-treatment of NC9 decreased the area of fibrin deposition and the proportion of CD41+ CD31+ cells in WT mice. These results suggest that TG2 prevents from pulmonary haemorrhage in ALI by promoting the adhesion of platelets to ECs and the fibrin deposition., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
30. Perineural treatment with anti-TNF-α antibody ameliorates persistent allodynia and edema in novel mouse models with complex regional pain syndrome.
- Author
-
Shibata S, Tagashira H, Nemoto T, Kita S, Kita T, Shinoda Y, Akiyoshi K, Yamaura K, and Iwamoto T
- Subjects
- Rats, Mice, Animals, Tumor Necrosis Factor Inhibitors therapeutic use, Tumor Necrosis Factor-alpha, Rats, Sprague-Dawley, Cytokines, Edema drug therapy, Disease Models, Animal, Hyperalgesia drug therapy, Complex Regional Pain Syndromes drug therapy
- Abstract
Complex regional pain syndrome (CRPS) is an intractable chronic pain syndrome with various signs and symptoms including allodynia/hyperalgesia, edema, swelling, and skin abnormalities. However, a definitive therapeutic treatment for CRPS has not been established. In CRPS patients, inflammatory cytokines such as TNF-α and IL-1β have been shown to increase in affected areas, suggesting that these molecules may be potential therapeutic targets for CRPS. Here, we first created a novel CRPS mouse model (CRPS-II-like) via sciatic nerve injury and cast immobilization, which was characterized by mechanical allodynia, local edema, and skin abnormalities, to evaluate the pathophysiology and pharmacotherapy of CRPS. When an anti-TNF-α antibody was consecutively administered near the injured sciatic nerve of CRPS model mice, persistent allodynia and CRPS-related signs in the ipsilateral hindpaw were markedly attenuated to control levels. Perineural administration of anti-TNF-α antibody also suppressed the upregulation of inflammatory cytokines as well as the activation of macrophages and Schwann cells in the injured sciatic nerve. These findings indicate that persistent allodynia and CRPS-related signs in CRPS models are primarily associated with TNF-α-mediated immune responses in injured peripheral nerves, suggesting that perineural treatment with anti-TNF-α antibody might be therapeutically useful., Competing Interests: Declaration of competing interest The authors have no conflict of interest regarding this manuscript., (Copyright © 2023 The Authors. Production and hosting by Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
31. A case of atrial septal defect with right-to-left shunting without pulmonary hypertension.
- Author
-
Yamazaki Y, Numasawa Y, Mase T, Arai M, Maeda T, Shinoda Y, Watabe K, Ono S, Hashimoto R, Yokokura S, Haginiwa S, Kojima H, Tanaka M, and Koizumi K
- Abstract
We report a case of a 54-year-old man with atrial septal defect who presented with oxygen desaturation on pulse oximetry. Cardiac magnetic resonance imaging and transesophageal echocardiography showed right-to-left shunting through an atrial septal defect, which was confirmed by superior vena cavography and suggested Eisenmenger syndrome. However, cardiac catheterization revealed a normal pulmonary arterial pressure. Simultaneous measurement of interatrial pressure identified two transient interatrial pressure gradient points, where the right atrial pressure was higher than the left atrial pressure. The patient was finally diagnosed with atrial septal defect without pulmonary hypertension. Right-to-left shunting was primarily caused by a transient interatrial pressure gradient due to a time delay in both initial atrial contraction and completion of passive ventricular filling between the right and left sides of the heart. Surgical closure of the atrial septal defect was performed, and hypoxemia improved. This is the first report of right-to-left shunting without pulmonary hypertension caused by a transient interatrial pressure gradient due to a time delay between the right and left cardiac cycles. Precise assessment of the simultaneous interatrial pressure in addition to diagnostic imaging played a pivotal role in clarifying the etiology of this rare condition., Learning Objective: Atrial septal defect with right-to-left shunting without Eisenmenger syndrome is a rare condition. We identified transient interatrial pressure gradients associated with a time delay in both initial atrial contraction and completion of the passive ventricular filling phase, which we considered as the primary mechanism underpinning right-to-left shunting. Simultaneous measurement of interatrial pressure played a pivotal role in elucidating the hemodynamics and abnormal shunt flow mechanism., Competing Interests: The authors declare that there is no conflict of interest., (© 2023 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
32. The current state of family caregiver burden and support of toilet problems for elderly with mild cognitive impairment and Alzheimer's disease.
- Author
-
Kamiya M, Osawa A, Shinoda Y, Nishii H, and Kondo I
- Subjects
- Humans, Aged, Caregivers psychology, Caregiver Burden, Psychiatric Status Rating Scales, Cost of Illness, Alzheimer Disease diagnosis, Alzheimer Disease psychology, Bathroom Equipment, Cognitive Dysfunction epidemiology, Cognitive Dysfunction psychology
- Abstract
Objective: To conduct an exploratory examination of caregiver burden involving toilet problems in patients with mild cognitive impairment (MCI) and Alzheimer's disease (AD) and related characteristics., Methods: We included 50 outpatients with amnestic MCI and AD and their caregivers. Patients were subclassified into three groups: MCI, mild AD, and moderate/severe AD. We used the Japanese version of the Zarit Burden Interview (J-ZBI) to evaluate caregiver burden and conducted a questionnaire on the frequency of lower urinary tract symptoms and related caregiver burden. We compared the frequency of questionnaire items with the level of burden in each group and subsequently determined the J-ZBI correlation coefficient., Results: Among the questionnaire items, the caregiver's burden of "increased daytime urinary frequency," "nocturia," "urinary incontinence," and "they cannot clean the toilet" statistically significantly correlated with J-ZBI scores (ρ = 0.52, 0.65, 0.79, and 0.83, respectively). Items including "they cannot clean the toilet," "the smell of excrement is bothersome," "assistance for transfer is necessary," "they soil the clothes and bed," and "they cannot clean the genital region" were significantly more common in the patient group with moderate/severe AD., Conclusions: Lower urinary tract symptoms and toilet problems were significantly correlated with caregiver burden. Toilet problems differ depending on the severity of dementia. Therefore, a support system based on dementia severity is required to address toilet problems., (© 2023 The Japanese Urological Association.)
- Published
- 2023
- Full Text
- View/download PDF
33. Tissue transglutaminase exacerbates renal fibrosis via alternative activation of monocyte-derived macrophages.
- Author
-
Shinoda Y, Tatsukawa H, Yonaga A, Wakita R, Takeuchi T, Tsuji T, Tanaka M, Suganami T, and Hitomi K
- Subjects
- Humans, Animals, Mice, Macrophages, Monocytes, Kidney, Protein Glutamine gamma Glutamyltransferase 2, Kidney Diseases
- Abstract
Macrophages are important components in modulating homeostatic and inflammatory responses and are generally categorized into two broad but distinct subsets: classical activated (M1) and alternatively activated (M2) depending on the microenvironment. Fibrosis is a chronic inflammatory disease exacerbated by M2 macrophages, although the detailed mechanism by which M2 macrophage polarization is regulated remains unclear. These polarization mechanisms have little in common between mice and humans, making it difficult to adapt research results obtained in mice to human diseases. Tissue transglutaminase (TG2) is a known marker common to mouse and human M2 macrophages and is a multifunctional enzyme responsible for crosslinking reactions. Here we sought to identify the role of TG2 in macrophage polarization and fibrosis. In IL-4-treated macrophages derived from mouse bone marrow and human monocyte cells, the expression of TG2 was increased with enhancement of M2 macrophage markers, whereas knockout or inhibitor treatment of TG2 markedly suppressed M2 macrophage polarization. In the renal fibrosis model, accumulation of M2 macrophages in fibrotic kidney was significantly reduced in TG2 knockout or inhibitor-administrated mice, along with the resolution of fibrosis. Bone marrow transplantation using TG2-knockout mice revealed that TG2 is involved in M2 polarization of infiltrating macrophages derived from circulating monocytes and exacerbates renal fibrosis. Furthermore, the suppression of renal fibrosis in TG2-knockout mice was abolished by transplantation of wild-type bone marrow or by renal subcapsular injection of IL4-treated macrophages derived from bone marrow of wild-type, but not TG2 knockout. Transcriptome analysis of downstream targets involved in M2 macrophages polarization revealed that ALOX15 expression was enhanced by TG2 activation and promoted M2 macrophage polarization. Furthermore, the increase in the abundance of ALOX15-expressing macrophages in fibrotic kidney was dramatically suppressed in TG2-knockout mice. These findings demonstrated that TG2 activity exacerbates renal fibrosis by polarization of M2 macrophages from monocytes via ALOX15., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
34. Recurrent glioblastoma metastatic to the lumbar vertebra: A case report and literature review: Surgical oncology.
- Author
-
Matsuhashi A, Tanaka S, Takami H, Nomura M, Ikemura M, Matsubayashi Y, Shinoda Y, Yamada K, Sakai Y, Karasawa Y, Takayanagi S, and Saito N
- Abstract
Background: Glioblastoma is a malignant tumor, and its prognosis is as poor as 1.5 to 2 years. Most cases recur within one year even under the standard treatment. The majority of recurrences are local, and in rare cases, metastasize mostly within the centra nervous system. Extradural metastasis of glioma is exceedingly rare. Here, we present a case of vertebral metastasis of glioblastoma., Case Presentation: We present a 21-year-old man post total resection of the right parietal glioblastoma, diagnosed with lumbar metastasis. He originally presented with impaired consciousness and left hemiplegia and underwent gross total resection of the tumor. Given the diagnosis of glioblastoma, he was treated with radiotherapy combined with concurrent and adjuvant temozolomide. Six months after tumor resection, the patient presented with severe back pain, and was diagnosed as metastatic glioblastoma on the first lumbar vertebrae. Posterior decompression with fixation and postoperative radiotherapy were conducted. He went on to receive temozolomide and bevacizumab. However, at 3 months after the diagnosis of lumbar metastasis, further disease progression was noted, and his care was transitioned to best supportive care. Comparison on copy number status between primary and metastatic lesions on methylation array analysis revealed more enhanced chromosomal instability including 7p loss, 7q gain and 8 gain in the metastatic lesion., Conclusion: Based upon the literature review and our case, younger age of initial presentation, multiple surgical interventions, and long overall survival seem to be the risk factors of vertebral metastasis. As the prognosis of glioblastoma improves over time, its vertebral metastasis is seemingly more common. Therefore, extradural metastasis should be kept in mind in the treatment of glioblastoma. Further, detailed genomic analysis on multiple paired specimens is mandated to elucidate the molecular mechanisms of vertebral metastasis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Matsuhashi, Tanaka, Takami, Nomura, Ikemura, Matsubayashi, Shinoda, Yamada, Sakai, Karasawa, Takayanagi and Saito.)
- Published
- 2023
- Full Text
- View/download PDF
35. Sigma-1 receptor is involved in modification of ER-mitochondria proximity and Ca 2+ homeostasis in cardiomyocytes.
- Author
-
Tagashira H, Bhuiyan MS, Shinoda Y, Kawahata I, Numata T, and Fukunaga K
- Subjects
- Animals, Mice, Rats, Homeostasis genetics, Mitochondria, Myocytes, Cardiac metabolism, Endoplasmic Reticulum metabolism, Calcium metabolism, Sigma-1 Receptor, Heart Failure, Receptors, sigma genetics, Receptors, sigma metabolism
- Abstract
The Sigma-1 receptor (Sigmar1) is downregulated in heart failure model mice with mitochondrial dysfunction. However, the mechanism in detail has not been investigated. In this study, we investigated the role of Sigmar1 in ER-mitochondria proximity using Sigmar1-knockdown or -overexpressed neonatal rat ventricular myocytes (NRVMs). The endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy was aggravated with the dysregulation of mitochondrial function and ER-mitochondrial junctional formation in Sigmar1-knockdown NRVMs, whereas improved in Sigmar1 overexpressed NRVMs. Our data suggests that the reduction of the cardiac Sigmar1 results in decrease mitochondrial Ca
2+ influx and promotes mitochondrial fission, followed by reduced ER-mitochondria proximity, exacerbating ET-1-induced cardiomyocyte injury., Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest., (Copyright © 2022 The Authors. Production and hosting by Elsevier B.V. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
36. Effect of Equibiaxial Pre-Stress on Mechanical Properties Evaluated Using Depth-Sensing Indentation with a Point-Sharp Indenter.
- Author
-
Akatsu T, Tabata Y, Shinoda Y, and Wakai F
- Abstract
This study examined the effect of an imposed equibiaxial pre-stress (EBPS) on the evaluation of mechanical properties, using the depth-sensing indentation method with a point-sharp indenter, through a numerical analysis of indentations simulated with the 3D finite element method. The predicted elastic modulus, E
* , and yield stress, Y* , were used as elastic and plastic deformation resistances under the indentation, respectively. It was found that both increased nominally with the increase in compressive EBPS and decreased with the increase in tensile EBPS, even though the induced change in the piling-up or sinking-in around the indentations was not significant. The effect of EBPS on E* was described by the Hooke's law for an isotropic elastoplastic material, whereas that on Y* was accounted for by the change in the von Mises stress due to EBPS.- Published
- 2023
- Full Text
- View/download PDF
37. Pathogenesis of selective damage of granule cell layer in cerebellum of rats exposed to methylmercury.
- Author
-
Du K, Hirooka T, Sasaki Y, Yasutake A, Hara T, Yamamoto C, Fujiwara Y, Shinoda Y, Fujie T, Katsuda S, Eto K, and Kaji T
- Subjects
- Rats, Animals, Tumor Necrosis Factor-alpha metabolism, Cerebellum metabolism, Neurons, Apoptosis, Methylmercury Compounds toxicity, Methylmercury Compounds metabolism
- Abstract
Granule cell-selective toxicity of methylmercury in the cerebellum is one of the main unresolved issues in the pathogenesis of Minamata disease. Rats were orally administered methylmercury chloride (10 mg/kg/day) for 5 consecutive days, and their brains were harvested on days 1, 7, 14, 21, or 28 after the last administration for histological examination of the cerebellum. It was found that methylmercury caused a marked degenerative change to the granule cell layers but not to the Purkinje cell layers. The generative change of the granule cell layer was due to cell death, including apoptosis, which occurred at day 21 and beyond after the methylmercury administration. Meanwhile, cytotoxic T-lymphocytes and macrophages had infiltrated the granule cell layer. Additionally, granule cells are shown to be a cell type susceptible to TNF-α. Taken together, these results suggest that methylmercury causes small-scale damage to granule cells, triggering the infiltration of cytotoxic T-lymphocytes and macrophages into the granule cell layer, which secrete tumor necrosis factor-α (TNF-α) to induce apoptosis in granule cells. This chain is established based on the susceptibility of granule cells to methylmercury, the ability of cytotoxic T lymphocytes and macrophages to synthesize and secrete TNF-α, and the sensitivity of granule cells to TNF-α and methylmercury. We propose to call the pathology of methylmercury-induced cerebellar damage the "inflammation hypothesis."
- Published
- 2023
- Full Text
- View/download PDF
38. Potential Association between Methylmercury Neurotoxicity and Inflammation.
- Author
-
Shinoda Y, Akiyama M, and Toyama T
- Subjects
- Humans, Inflammation chemically induced, Astrocytes, Central Nervous System, Methylmercury Compounds toxicity, Neurotoxicity Syndromes etiology
- Abstract
Methylmercury (MeHg) is the causal substrate of Minamata disease and a major environmental toxicant. MeHg is widely distributed, mainly in the ocean, meaning its bioaccumulation in seafood is a considerable problem for human health. MeHg has been intensively investigated and is known to induce inflammatory responses and neurodegeneration. However, the relationship between MeHg-induced inflammatory responses and neurodegeneration is not understood. In the present review, we first describe recent findings showing an association between inflammatory responses and certain MeHg-unrelated neurological diseases caused by neurodegeneration. In addition, cell-specific MeHg-induced inflammatory responses are summarized for the central nervous system including those of microglia, astrocytes, and neurons. We also describe MeHg-induced inflammatory responses in peripheral cells and tissue, such as macrophages and blood. These findings provide a concept of the relationship between MeHg-induced inflammatory responses and neurodegeneration, as well as direction for future research of MeHg-induced neurotoxicity.
- Published
- 2023
- Full Text
- View/download PDF
39. Analysis of pharmacological interventions among hospitalised patients with COVID‑19: A focus on severe cases.
- Author
-
Shinoda Y, Ohashi K, Matsuoka T, and Yoshimura T
- Abstract
Existing recommendations regarding pharmaceutical interventions for patients with coronavirus disease 2019 (COVID-19) focus on outpatient, inpatient and post-discharge care. However, there are no studies examining the actual activities of pharmacists in relation to hospitalised patients. The present study aimed to identify pharmacists' roles by analysing cases of pharmaceutical interventions, particularly for patients admitted to high-care units. Pharmacological interventions were provided to patients with severe COVID-19 or patients at high risk of severe disease in 2021. These pharmaceutical interventions were analysed and evaluated. Pharmacists also developed a COVID-19 drug compatibility chart for use by care team members. In the present study, 54 patients were included, of which 33 were severe cases. A total of 28 patients (52%) received pharmacological interventions and 25 of them were severe cases. Out of 68 pharmacological interventions, interventions for antimicrobial agents were the most common (28 interventions), followed by nutrition and anti-COVID-19 drug-related interventions. In addition, the need for interventions relating to drug compatibility was reduced by ~43% after the drug compatibility chart was implemented. In conclusion, pharmacists have a responsibility to improve the quality of pharmacotherapy for patients with COVID-19. They should focus on creating specific pharmacotherapy tools for patients with COVID-19 and supporting appropriate antimicrobial use for secondary bacterial infections., Competing Interests: The authors declare that they have no competing interests., (Copyright © 2020, Spandidos Publications.)
- Published
- 2022
- Full Text
- View/download PDF
40. Predictive Factors for Oral Intake Recovery After Acute Stroke: Analysis of a Japanese Nationwide Inpatient Database.
- Author
-
Inooka Y, Yamana H, Shinoda Y, Inokuchi H, Matsui H, Fushimi K, Yasunaga H, and Haga N
- Subjects
- Male, Female, Humans, Japan, Inpatients, Eating, Retrospective Studies, Deglutition Disorders complications, Stroke complications
- Abstract
Objective: It remains unclear which factors contribute to dysphagia recovery after an acute stroke. This study aimed to identify factors associated with complete oral intake recovery in patients with post-stroke dysphagia., Methods: Data were obtained from the Diagnosis Procedure Combination database, which is a nationwide database of administrative claims and discharge abstracts in Japan. We selected patients admitted within 3 days of stroke onset and conducted multivariable logistic regression analysis. Total oral intake within 30 days of admission was the primary outcome measure., Results: A total of 151,302 patients were included, and total oral intake was observed in 48% of them within 30 days of admission. Total oral intake was significantly associated with age, sex, stroke subtype, consciousness disturbance, low body mass index, multiple comorbidities, and the modified Rankin Scale before stroke onset. In addition, males had worse outcomes than females (odds ratio, 0.75; 95% confidence interval, 0.73-0.77, p < 0.001), and intracerebral and subarachnoid hemorrhagic stroke subtypes were associated with non-recovery., Conclusion: This study identified several prognostic factors for total oral intake in patients with acute stroke. These results may be useful for predicting a patient's dysphagia prognosis at the time of admission and designing a nutritional management plan for patients with acute stroke., (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2022
- Full Text
- View/download PDF
41. Loss of CAPS2/Cadps2 leads to exocrine pancreatic cell injury and intracellular accumulation of secretory granules in mice.
- Author
-
Sato Y, Tsuyusaki M, Takahashi-Iwanaga H, Fujisawa R, Masamune A, Hamada S, Matsumoto R, Tanaka Y, Kakuta Y, Yamaguchi-Kabata Y, Furuse T, Wakana S, Shimura T, Kobayashi R, Shinoda Y, Goitsuka R, Maezawa S, Sadakata T, Sano Y, and Furuichi T
- Abstract
The type 2 Ca
2+ -dependent activator protein for secretion (CAPS2/CADPS2) regulates dense-core vesicle trafficking and exocytosis and is involved in the regulated release of catecholamines, peptidergic hormones, and neuromodulators. CAPS2 is expressed in the pancreatic exocrine acinar cells that produce and secrete digestive enzymes. However, the functional role of CAPS2 in vesicular trafficking and/or exocytosis of non-regulatory proteins in the exocrine pancreas remains to be determined. Here, we analyzed the morpho-pathological indicators of the pancreatic exocrine pathway in Cadps2 -deficient mouse models using histochemistry, biochemistry, and electron microscopy. We used whole exosome sequencing to identify CADPS2 variants in patients with chronic pancreatitis (CP). Caps2/Cadps2 -knockout (KO) mice exhibited morphophysiological abnormalities in the exocrine pancreas, including excessive accumulation of secretory granules (zymogen granules) and their amylase content in the cytoplasm, deterioration of the fine intracellular membrane structures (disorganized rough endoplasmic reticulum, dilated Golgi cisternae, and the appearance of empty vesicles and autophagic-like vacuoles), as well as exocrine pancreatic cell injury, including acinar cell atrophy, increased fibrosis, and inflammatory cell infiltration. Pancreas-specific Cadps2 conditional KO mice exhibited pathological abnormalities in the exocrine pancreas similar to the global Cadps2 KO mice, indicating that these phenotypes were caused either directly or indirectly by CAPS2 deficiency in the pancreas. Furthermore, we identified a rare variant in the exon3 coding region of CADPS2 in a non-alcoholic patient with CP and showed that Cadps2-dex3 mice lacking CAPS2 exon3 exhibited symptoms similar to those exhibited by the Cadps2 KO and cKO mice. These results suggest that CAPS2 is critical for the proper functioning of the pancreatic exocrine pathway, and its deficiency is associated with a risk of pancreatic acinar cell pathology., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Sato, Tsuyusaki, Takahashi-Iwanaga, Fujisawa, Masamune, Hamada, Matsumoto, Tanaka, Kakuta, Yamaguchi-Kabata, Furuse, Wakana, Shimura, Kobayashi, Shinoda, Goitsuka, Maezawa, Sadakata, Sano and Furuichi.)- Published
- 2022
- Full Text
- View/download PDF
42. Impact of Sex in Left Atrial Indices for Prognosis of Heart Failure with Preserved Ejection Fraction.
- Author
-
Hoshida S, Tachibana K, Masunaga N, Shinoda Y, Minamisaka T, Inui H, Ueno K, Seo M, Yano M, Hayashi T, Nakagawa A, Nakagawa Y, Tamaki S, Yamada T, Yasumura Y, Sotomi Y, Hikoso S, Nakatani D, and Sakata Y
- Abstract
Objective: We aim to clarify the differences in the association between re-admission for heart failure (HF) and left atrial (LA) overload indices by sex in heart failure and a preserved ejection fraction (HFpEF)., Methods: We analyzed 898 HFpEF patients hospitalized for acute decompensated HF. Blood tests and transthoracic echocardiography were performed before discharge. The primary endpoint was re-admission for HF during the first year., Results: The ratio of diastolic elastance to arterial elastance ( p = 0.014), a relative index of LA pressure overload, in men and LA volume index (LAVI, p = 0.020) in women were significant for re-admission for HF during the first year in the multivariable Fine-Gray analysis. Stroke volume (SV)/LA volume (LAV), another index for LAV overload, was not a significant prognostic factor of re-admission for HF during this time., Conclusion: LA overload was an important prognostic factor for HF re-readmission during the first year after enrolment in patients with HFpEF, but the indices relating to LA overload differed by sex.
- Published
- 2022
- Full Text
- View/download PDF
43. Impact of continuous pharmacist intervention for injectable antimicrobials on the treatment of patients with Escherichia coli bacteremia.
- Author
-
Shinoda Y, Ohashi K, Matsuoka T, Arai K, Hotta N, Asano I, and Yoshimura T
- Subjects
- Anti-Bacterial Agents therapeutic use, Escherichia coli, Humans, Pharmacists, Anti-Infective Agents therapeutic use, Bacteremia drug therapy, Escherichia coli Infections drug therapy
- Abstract
Background: A prospective audit with intervention and feedback (PAF) by pharmacists is important for the appropriate use of antimicrobials. Clinically, Escherichia coli (E. coli) bacteremia is a common condition, but only few researchers have examined the role of PAF in its appropriate use of antimicrobials., Methods: We started PAF by pharmacists in 2017 for all injectable antibiotics. This study included cases of E. coli bacteremia that resulted in hospitalization over a 4 year period from 2016 to 2019. Patients were grouped by year (Period 0-3), and clinical outcomes were examined., Results: The pharmacists had 12 and 54 suggestions in Periods 0 and 3, respectively. The most common suggestion was de-escalation. The median duration of antimicrobial use was 12 (interquartile range: 8-15) days in Periods 0-2. The duration of antimicrobial use was significantly reduced to 9 (7-12) days in Period 3. In Period 3, the duration of antimicrobial use was reduced by 29%, while anti-pseudomonal drug use was reduced by 42% compared with that in period 0. The 30 day mortality rates were not significantly different between the groups., Conclusions: PAF by pharmacists promotes the appropriate use of antimicrobials in patients with E. coli bacteremia; it is important to continue the program for several years., (Copyright © 2022 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
44. Liposarcoma With Hibernoma-like Histology: A Clinicopathologic Study of 16 Cases.
- Author
-
Kojima N, Komiyama M, Shinoda Y, Watanabe SI, Yatabe Y, Kawai A, and Yoshida A
- Subjects
- Biopsy, Cell Differentiation, Humans, Proto-Oncogene Proteins c-mdm2 genetics, Uncoupling Protein 1, Lipoma pathology, Liposarcoma chemistry, Liposarcoma, Myxoid pathology
- Abstract
Hibernoma is an uncommon benign tumor of brown fat cells that consistently expresses uncoupling protein 1 (UCP1). Herein, we clinicopathologically characterized 16 liposarcomas, for which histology, at least focally, closely resembled that of hibernoma, including sheets of brown fat-like, finely multivacuolated-to-eosinophilic tumor cells with no or minimal nuclear atypia. The cohort consisted of 4 well-differentiated liposarcomas (WDLSs), 6 dedifferentiated liposarcomas with a concomitant WDLS component, and 6 myxoid liposarcomas (MLSs). For all dedifferentiated liposarcoma cases, hibernoma-like histology was present only in the WDLS component. All tumors presented as large, deep-seated masses. Hibernoma-like histology resembled the pale cell, mixed cell, eosinophilic cell, or spindle cell subtypes of hibernoma, and it was a focal observation, with conventional liposarcoma histology coexisting in all cases. However, a few biopsy samples were predominated by hibernoma-like patterns, and 1 case was initially interpreted as hibernoma. Hibernoma-like components in WDLS immunohistochemically coexpressed MDM2 and CDK4 in most cases and harbored MDM2 amplification in tested cases, whereas half of the cases expressed UCP1. The hibernoma-like components of MLS expressed DDIT3, and DDIT3 rearrangements were present in the tested cases, whereas only negative or equivocal UCP1 expression was observed. In summary, WDLS and MLS focally demonstrate hibernoma-like histology on rare occasions. These elements are neoplastic, and some such areas in WDLS likely represent true brown fat differentiation, as supported by UCP1 expression. This pattern requires recognition to avoid the misdiagnosis as hibernoma, especially in biopsies. A careful search for classic liposarcoma histology and additional work-ups for the MDM2/CDK4 or DDIT3 status will be helpful for an accurate diagnosis., Competing Interests: Conflicts of Interest and Source of Funding: Supported in part by JSPS KAKENHI (Grant Number JP21K06919, A.Y.). The authors have disclosed that they have no significant relationships with, or financial interest in, any commercial companies pertaining to this article., (Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
45. Can Outpatient Rehabilitation Be Continued During the COVID-19 Pandemic? A Report from a Japanese Regional Medical University Hospital.
- Author
-
Yamanouchi Y, Maeda K, Shinoda Y, Majima M, Lee J, Inoue I, Maruyama Y, and Kurabayashi H
- Abstract
Objective: To describe the operation of an outpatient rehabilitation practice at a Japanese hospital severely affected by the coronavirus disease 2019 (COVID-19) pandemic., Design: Analytical observational study., Setting: Outpatient rehabilitation department in Saitama, Japan., Participants: Number (N=953) of outpatients from January 2019 to July 2021., Interventions: Not applicable., Main Outcome Measures: This paper begins with a review of the infection control measures that were initiated after declaration of a state of emergency in April 2020. The effects of the pandemic were then examined by comparing the daily average number of outpatients from January 2020 to July 2021 with that noted for the same duration during 2019., Results: In April 2020, the average daily number of patients decreased by 77.1% compared with the number in 2019 and was further decreased by 65.7% and 63.7% in May and June 2020, respectively. The time limitations on rehabilitation were lifted in June, and the number of patients increased by 82.3% in July 2020. Thereafter, it remained at approximately 80% throughout the rest of the year compared with that noted in 2019. From January 2021 to July 2021, the number of patients approached the number noted during normal practice or was even higher., Conclusions: The implementation of infection control measures, adjustments to procedures, and widespread vaccination permitted the continuation of our outpatient practice., (© 2022 The Authors.)
- Published
- 2022
- Full Text
- View/download PDF
46. Time-sensitive prognostic performance of an afterload-integrated diastolic index in heart failure with preserved ejection fraction: a prospective multicentre observational study.
- Author
-
Hoshida S, Hikoso S, Shinoda Y, Tachibana K, Minamisaka T, Shunsuke T, Yano M, Hayashi T, Nakagawa A, Nakagawa Y, Yamada T, Yasumura Y, Nakatani D, and Sakata Y
- Subjects
- Aged, Female, Heart Ventricles, Humans, Male, Prognosis, Prospective Studies, Stroke Volume physiology, Ventricular Function, Left physiology, Heart Failure complications, Heart Failure diagnostic imaging
- Abstract
Objectives: The prognostic significance of an afterload-integrated diastolic index, the ratio of diastolic elastance (Ed) to arterial elastance (Ea) (Ed/Ea=[E/e']/[0.9×systolic blood pressure]), is valid for 1 year after discharge in older patients with heart failure with preserved ejection fraction (HFpEF). We aimed to clarify the association with changes in Ed/Ea from enrolment to 1 year and prognosis thereafter in patients with HFpEF., Setting: A prospective, multicentre observational registry of collaborating hospitals in Osaka, Japan., Participants: We enrolled 659 patients with HFpEF hospitalised for acute decompensated heart failure (men/women: 296/363). Blood tests and transthoracic echocardiography were performed before discharge and at 1 year after., Primary Outcome Measures: All-cause mortality and/or re-admission for heart failure were evaluated after discharge., Results: High Ed/Ea assessed before discharge was a significant prognostic factor during the first, but not the second, year after discharge in all-cause mortality or all-cause mortality and/or re-admission for heart failure. When re-analysis was performed using the value of Ed/Ea at 1 year after discharge, high Ed/Ea was significant for the prognosis during the second year for both end points (p=0.012 and p=0.033, respectively). The poorest mortality during 1‒2 years after enrolment was observed in those who showed a worsening Ed/Ea during the first year associated with larger left ventricular mass index and reduced left ventricular ejection fraction. In all-cause mortality and/or re-admission for heart failure, the event rate during 1‒2 years was highest in those with persistently high Ed/Ea even after 1 year., Conclusions: Time-sensitive prognostic performance of Ed/Ea, an afterload-integrated diastolic index, was observed in older patients with HFpEF., Trial Registration Number: UMIN000021831., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2022
- Full Text
- View/download PDF
47. Essential thrombocythemia complicated with simultaneous two-vessel acute myocardial infarction in the subacute phase of takotsubo cardiomyopathy: A case report.
- Author
-
Yamazaki Y, Numasawa Y, Mase T, Maeda T, Shinoda Y, Watabe K, Ono S, Naito A, Yokokura S, Haginiwa S, Kojima H, and Tanaka M
- Abstract
We report the case of a 79-year-old woman with essential thrombocythemia who presented with simultaneous two-vessel acute myocardial infarction (AMI) in the subacute phase of takotsubo cardiomyopathy. Despite sufficient anticoagulation therapy with warfarin to prevent thrombus formation in the left ventricle, the patient developed simultaneous two-vessel AMI in the right and left circumflex coronary arteries 16 days after the onset of takotsubo cardiomyopathy. Thromboembolism from the left ventricle associated with takotsubo cardiomyopathy was considered a potential cause of this event. However, macroscopic and pathological findings of the aspirated thrombi revealed that the primary cause of AMI was non-organized white platelet thrombi associated with essential thrombocythemia. In addition to oral anticoagulation therapy with warfarin, low-dose aspirin was started. The patient was discharged without any symptoms, and the clinical course has been uneventful for >5 years. This case highlights the potential risk of fatal complications associated with essential thrombocythemia, including simultaneous multivessel AMI. Additionally, pathological findings of the thrombi may play a crucial role in clarifying the etiology in such complicated cases. Appropriate antithrombotic therapy should be selected according to the pathogenesis of the condition., Learning Objective: We describe a 79-year-old woman with essential thrombocythemia complicated with simultaneous two-vessel acute myocardial infarction (AMI) in the subacute phase of takotsubo cardiomyopathy. Although patients with essential thrombocythemia are highly predisposed to thrombotic events including AMI, the appropriate antithrombotic regimen remains controversial. The macroscopic and pathological findings of the thrombi play a pivotal role in clarifying the etiology, which may lead to the appropriate antithrombotic therapy., Competing Interests: The authors declare that there is no conflict of interest., (© 2022 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
48. Imaging features inferring symptom onset due to spinal metastasis progression: a preliminary study.
- Author
-
Ando M, Sumitani M, Fuyuki M, Abe H, Shinoda Y, Matsubayashi Y, Oshima Y, Inoue R, Tsuchida R, and Uchida K
- Subjects
- Activities of Daily Living, Cross-Sectional Studies, Humans, Paralysis, Quality of Life, Neuralgia etiology, Nociceptive Pain complications, Spinal Neoplasms diagnostic imaging, Spinal Neoplasms secondary
- Abstract
Background: Spinal metastases can cause intractable pain and neurological deficits, which can markedly worsen both patients' activities of daily living (ADL) and their health-related quality of life (QOL). Early intervention is essential to prevent irreversible neurological deficits and pain associated with spinal metastases. We investigated the imaging features of spinal metastases that led to neurological deficits., Methods: We analyzed axial cross-sectional computed tomography (CT) images of cervical and thoracic spinal metastases in patients with and without lower limb motor paralysis, neuropathic pain, and local nociceptive pain. We distinguished regions of the spine associated with these respective symptoms, and explored their inferable performance using images obtained before symptom onset. In addition, we analyzed the imaging features and type of bone metastasis (osteolytic and osteoblastic)., Results: Spinal lesions occupied the area in and around the spinal canal and around the pedicle in patients with motor paralysis. Lesions around the pedicle and in the most posterior vertebral body part before symptom onset were inferable. In patients with neuropathic pain, spinal metastases spread along the pedicle before symptom onset, and had surrounded the spinal canal circumferentially at symptom onset. Local nociceptive pain was more common near the center of the vertebral body either at or before symptom onset. There was no difference in the imaging features according to the type of bone metastasis., Conclusions: Lesions in certain regions in the asymptomatic metastatic spine can indicate the onset of spinal metastasis-related symptoms in the next few months. Early therapeutic intervention might be applied to prevent neurological disorder.
- Published
- 2022
- Full Text
- View/download PDF
49. The clinical significance of perioperative inflammatory index as a prognostic factor for patients with retroperitoneal soft tissue sarcoma.
- Author
-
Matsui Y, Matsuda A, Maejima A, Shinoda Y, Nakamura E, Komiyama M, and Fujimoto H
- Subjects
- Albumins, C-Reactive Protein analysis, Hemoglobins analysis, Humans, Lipopolysaccharides, Lymphocytes, Neutrophils, Prognosis, Retrospective Studies, Retroperitoneal Neoplasms surgery, Sarcoma surgery, Soft Tissue Neoplasms
- Abstract
Background: The prognostic factors of retroperitoneal soft tissue sarcoma (STS) have been explored but not yet certain. This study evaluated the prognostic impact of various preoperative clinical parameters and inflammatory indices in primary STS, with a particular focus on the transition of inflammatory index before and after tumor resection in de-differentiated liposarcoma (DD-LPS)., Methods: The clinical data of 113 patients with primary retroperitoneal STS receiving tumor resection were reviewed. Six variables (neutrophils, platelets, C-reactive protein (CRP), lymphocytes, albumin, and hemoglobin) in the blood samples were measured and nine inflammatory indices (neutrophil-lymphocyte ratio (NLR), CRP-lymphocyte ratio (CLR), platelet-lymphocyte ratio (PLR), neutrophil-albumin ratio (NAR), CRP-albumin ratio (CAR), platelet-albumin ratio (PAR), HALP (hemoglobin, albumin, lymphocyte and platelet), prognostic nutrition index (PNI), and modified Glasgow Prognostic Score (mGPS)) were calculated. The prognostic value of the indices was analyzed by univariate and multivariate analyses., Results: Elevated NLR, CLR, PLR, NAR, CAR, PAR, and mGPS were associated with a worse overall survival (p = 0.0124, 0.0011, 0.049, 0.0047, 0.0085, 0.0332, and 0.0086, respectively) in univariate analysis. Multivariate analysis showed that elevated CLR and DD-LPS were associated with poor overall survival (p = 0.0267 and 0.0218, respectively) in all retroperitoneal STS. In DD-LPD, patients with preoperative high CLR, whose postoperative CLR was normalized, demonstrated a favorable survival rate similar to those with preoperative low CLR., Conclusions: Elevated CLR before surgery as well as DD-LPS were poor prognostic markers for overall survival in primary retroperitoneal STS. Perioperative CLR normalization may be related to a favorable prognosis in DD-LPS., (© 2022. The Author(s) under exclusive licence to Japan Society of Clinical Oncology.)
- Published
- 2022
- Full Text
- View/download PDF
50. Catheter ablation of ventricular tachycardia in dilated-phase hypertrophic cardiomyopathy: Substrate characterization and ablation outcome.
- Author
-
Naeemah QJ, Komatsu Y, Nogami A, Sekiguchi Y, Igarashi M, Yamasaki H, Shinoda Y, Aonuma K, and Ieda M
- Subjects
- Endocardium, Female, Humans, Treatment Outcome, Cardiomyopathy, Dilated, Cardiomyopathy, Hypertrophic complications, Cardiomyopathy, Hypertrophic surgery, Catheter Ablation methods, Tachycardia, Ventricular
- Abstract
Introduction: Catheter ablation is a therapeutic option to suppress ventricular tachycardia (VT) in the setting of dilated-phase hypertrophic cardiomyopathy (DHCM). However, the characteristics of the arrhythmogenic substrate and the ablation outcome are not fully illustrated., Method: A total of 23 ablation procedures for drug-refractory sustained monomorphic VTs in 13 DHCM patients (60 ± 11 years, one female, the left ventricular [LV] ejection fraction 39% ± 9%, the LV mass index 156 ± 39 g/m
2 ) were performed. The distribution of VT substrate as endocardial or epicardial/intramural was based on detailed mapping and ablation response during VT., Result: Two patients underwent ablation of sustained monomorphic VT that was not scar-mediated tachycardia. Of the remaining 11 patients, eight (73%) patients had VT substrate in the basal regions, most frequently at the epicardial and/or intramural basal antero-septum. None of the patients had VT substrate located at the LV inferolateral region. Ablation at the right ventricular septum and the aortic cusps was done in four and five patients, respectively. Other approaches including bipolar and chemical ablations, were done in three and two patients, respectively. Six (55%) out of 11 patients (two patients lost follow-up) had VT recurrence. All the six patients had basal substrate. However, anti-tachycardia pacing was sufficient for VT termination except in one patient., Conclusion: Catheter ablation of VT in patients with DHCM is challenging because of the predominant basal anteroseptal epicardial/intramural location of arrhythmogenic substrate. An ablation approach from multiple sites and/or adjunctive interventional techniques are often required., (© 2022 Wiley Periodicals LLC.)- Published
- 2022
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.