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The Ningxiang pig, a distinguished local breed in China, is recognized for its good meat quality traits. This study examines the proteomics of Ningxiang pigs at three developmental stages and delves into the upstream transcriptomics of these proteomics. Such an analysis facilitates a deeper understanding of the molecular interplay between proteins and transcriptomes in the Ningxiang pig muscle, influencing muscle growth and development. In this research, we analyzed the muscles of Ningxiang pigs at three developmental stages: 30 days in weaned piglets, 90 days in nursery pigs, and 210 days in late fattening pigs. There a total of 16 differentially co-expressed miRNAs (ssc-miRNA-1, ssc-miRNA-378, ssc-miRNA-143, ssc-miRNA-30e, etc.), 74 differentially co-expressed mRNA (PLIN3, CPT2, IGF2 and HSP90AB1, etc.) have been identified in the three stages. 572 differentially abundant proteins (DAPs) (APOC3, NDUFA2, HSPD1, ATP5E, PDHA1, etc.) were readily identified by comparing different time periods. According to the KEGG enrich pathway results that DAPs most enriched in growth and development pathways, immune mechanism pathways and maintaining functions of physical. Through short time-series expression miner (STEM) association analysis, a total of 571 negative miRNA-mRNA interaction pairs and 2 negative miRNA-mRNA-protein (Chr05_11955-Pig.17268.1-ATP5F1B, ssc-miR-194a-3p-Pig.15802.1-ACY1) interaction pairs were found. Our study provides a theoretical basis on molecular mechanism for the study of IMF deposition, muscle growth and immunity in Ningxiang pig breed.

This experiment was conducted to investigate the effects of palygorskite and probiotics complex (Pal–Pro) supplementation on laying performance, the microflora structure and biodiversity of Xuefeng black-bone chicken. A total of 432 Xuefeng black-bone chickens were randomly assigned into 4 groups with 6 replicates of 18 hens. The control group (group A) was fed the basal diet, and the experimental groups (groups B, C and D) were fed basal diet supplemented with 250, 500 and 750 mg/kg Pal–Pro for 8 weeks. The results were as follows: (1) The Pal–Pro supplementation linearly increased (p

Th is study examined the eff ects of four levels of rumen-protected methionine (RPM) on the milk performance of dairy goats subjected to severe heat stress (temperature humidity index >90). Seventy-five Guanzhong dairy goats (52.6±4.9 kg) with the same farrowing period and lactation day (120±10 d) were randomly divided into five groups of 15 head and fed a 13.55% crude protein diet supplemented with 0, 1.5, 3, 4.5 or 6 g/d RPM in the concentrate. Th e trial included nine days adaptation time and 30 days of sampling and analysis. Th e addition of RPM did not change feed intake but 1.5 g/d RPM significantly increased milk protein content, the ratio of milk to feed, and the economic returns. No significant changes in milk fat or urea-nitrogen concentrations or somatic cell count were observed. Plasma ureanitrogen was significantly lowered in the 1.5 g/d RPM group. RPM supplementation increased plasma methionine concentration and total amino acid concentration in a dose-dependent manner. Th e highest dose of RPM (6 g/d) enhanced plasma immunoglobulin G concentration. It is demonstrated that supplementation with an appropriate dose of RPM to dairy goats fed a relatively low protein diet and subject to heat stress can increase milk protein production and improve economic returns.