85 results on '"Santos Y"'
Search Results
2. Microwave-Assisted Synthesis of β-Cyanoketones under Bucherer–Bergs Conditions and Their Antimicrobial Evaluation and In Silico Studies
- Author
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Leyva-Acuña, M. A., Delgado-Vargas, F., Lopez-Angulo, G., Ahumada-Santos, Y. P., Rivero, I. A., Durán-Pérez, S., and Montes-Avila, J.
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- 2023
- Full Text
- View/download PDF
3. Transportation and Urban Planning Under State Socialism
- Author
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Khairullina, Elvira, primary and Santos y Ganges, Luis, additional
- Published
- 2022
- Full Text
- View/download PDF
4. The towers of the Spanish optical telegraph. Antecedents and variants of the architectural type
- Author
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Luis Santos y Ganges and Laura Lalana-Encinas
- Subjects
optical telegraph ,telegraphic tower ,embrasure tower ,military architecture ,spain ,Engineering design ,TA174 ,Architectural engineering. Structural engineering of buildings ,TH845-895 - Abstract
The Spanish optical telegraph was planned by the Ministry of the Interior in 1844 for civilian governmental use. It was a ‘tower and telescope’ telegraph developed by Colonel José María Mathé, with a design clearly inspired by military sensibilities and considerations. This paper first analyses the contemporary military reference point of the embrasure tower as a military stronghold campaign. It goes on to examine the historical antecedents of watchtowers or signal towers from the 16th century and the freestanding or outpost towers from the 17th century. This is followed by a description of the architectural type of telegraph tower and an analysis of its variants, which gradually differed from the model typology in both form and materiality. Finally, it examines the military telegraph towers of the second and third Carlist wars. Thus, the historical and typological study emphasizes the military inspiration of the optical telegraph towers that has often gone unremarked in order to optimize the heritage characterization of the remaining structures.
- Published
- 2022
- Full Text
- View/download PDF
5. Implante de pr�tesis a�rtica transcat�ter en insuficiencia a�rtica no calcificada. �En qu� punto estamos?
- Author
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Amat-Santos y, Ignacio J., primary and Sánchez-Luna, Juan Pablo, additional
- Published
- 2024
- Full Text
- View/download PDF
6. FÍSICA E LITERATURA: UMA APROXIMAÇÃO ENTRE CIÊNCIA E ARTE
- Author
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SANTOS, Y. F. R., primary, FERREIRA, S. R. B., additional, FERREIRA, R. J. S., additional, MOUCHEREK, F. M. O., additional, and FERREIRA, W.S., additional
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- 2022
- Full Text
- View/download PDF
7. DETECÇÃO DE ENTEROBACTERIACEAE EM LEITE PASTEURIZADO E AVALIAÇÃO DA ATIVIDADE PROTEOLÍTICA
- Author
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Silva, A. L. F., primary, Rodrigues, A. B. B., additional, Monsores, M. R. F., additional, Amaral, P. V. V., additional, Almeida, V. N., additional, Santos, Y. C. O., additional, and Ramos, G. L. P., additional
- Published
- 2022
- Full Text
- View/download PDF
8. Open pit 3D slope stability for anisotropic rock masses in soft iron ore deposits
- Author
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Figueiredo, L.T., primary, Hammah, R., additional, Santos, Y., additional, Souza, T., additional, and Nogueira, C., additional
- Published
- 2021
- Full Text
- View/download PDF
9. Application on infrared spectroscopy for the analysis of total phenolic compounds in fruits.
- Author
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Santos, Y. J. S., Malegori, C., Colnago, L. A., and Vanin, F. M.
- Subjects
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INFRARED spectroscopy , *POISONS , *FRUIT , *BIOACTIVE compounds , *SUSTAINABLE chemistry - Abstract
Recent studies have demonstrated the metabolic benefits of phenolic compounds on human health. However, traditional analytical methods used for quantification of total phenolic compounds are time-consuming, laborious, require a high volume of reagents, mostly toxic substances, and involve several steps that can result in systematic and instrumental errors. Spectroscopic techniques have been used as alternatives to these methods for the determination of bioactive compounds directly in the food matrix by minimal sample preparation, without using toxic reagents. Therefore, this overview presents the advantages of nondestructive methods focusing on infrared spectroscopy (IR), for the quantification of total phenolic compounds in fruits. In addition, the main difficulties in applying these spectroscopic techniques are presented, as well as a comparison between the quantification of total phenolic compounds by traditional and IR methods. This review concludes by focusing on model building, highlighting that IR data are mainly processed using the partial least-squares (PLS) regression method to predict total phenolic content. The development of portable and inexpensive IR instruments, combined with multivariate data processing, could give to the consumers a straightforward technology to evaluate the total phenolic content of fruits prior to purchase. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
10. Historical Interrelationship of Railways and Cities from an Urban Viewpoint
- Author
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Luis Santos y Ganges
- Abstract
This paper offers a conceptual overview of the state of the art relating to the historical interrelationships between railways and cities from the point of view of town planning, covering the good century and a half that there have been railways in the Iberian Peninsula. It addresses both urban and railway history, reconsidering major issues in this relationship. These are: 1) the tracks of lines as they cross cities and the role they play in urban life, 2) the passenger buildings of stations, seen as a focus of urban centrality, 3) the developing complexity of cities and railways, together with the installation of new road and port infrastructures, and 4) the problems emerging from urban expansion in the final third of the twentieth century, mostly after the restoration of democracy, specifically the question of the limited permeability of rail tracks and the interpretation as a social barrier of the physical barrier thus constituted by the railway. An idea that railways are a problem grew up within this historical interrelationship.
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- 2023
11. TUMOUR GRADE REGRESSION (TRG) AND CORRELATION WITH MAGNETIC RESONANCE IMAGING (MRI) IN RECTAL CANCER
- Author
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Ocanto A, Dos Santos Y, Cantero R, Rodríguez I, and Morera R
- Abstract
Purpose: Colorectal cancer is the third most common cancer worldwide, neoadjuvant chemoradiotherapy (nCRT) is the standard treatment for locally advanced rectal cancer (LARC) and Magnetic Resonance Imaging (MRI) plays an important role in the management of this cases, determining the response to treatment but nowadays does not exist an unique criteria to unify tumour regression grade (TRG) and determinate possible patients for watch and wait and surgical option. 137 patients: 71 (51,8%) m Method: en and 66 (48,2%) women with newly diagnosis of LARC underwent to nCRT and evaluate locally with MRI were analyzed to determinate the circumferential resection margin (CRM), extramural vascular invasion (EMVI), satellite tumour deposit (STD), the state of the sphincter complex (SC) and mrTRG evaluated by experienced abdominal radiologist. Complete pathological response (cPR) was determinate in Result: 30 patients, 57,5% presented partial pathological response (pPR), CRM were 26,4%, EMVI were 18,4%, STD were 14,6% and SCI 6,9% all of them with statistical signicative (p
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- 2022
12. La selección de los polos industriales del plan de desarrollo de 1964: el ejemplo del Valle del Duero
- Author
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Miguel Fernández-Maroto and Luis Santos y Ganges
- Subjects
Urban Studies ,Geography, Planning and Development - Abstract
La selección de los polos industriales de la planificación del desarrollo en España es un asunto relativamente desconocido. La selección técnica de los polos del plan de desarrollo de 1964 fue llevada a cabo por la Comisaría del Plan de Desarrollo Económico y Social, con la asistencia interna de la Ponencia de Localización Geográfica y con la asesoría externa de consultoras privadas, aunque la decisión final fue tomada por el Consejo de Ministros, y no fue exactamente la misma. El estudio de la confusa definición de los ámbitos territoriales de análisis que condicionaron la selección de los polos, ilustrado con el caso concreto del valle del Duero, y de los factores vinculados a la selección de los dos polos que le correspondieron –Valladolid y Burgos– permite concluir que los criterios fueron poco claros y fluctuantes, y que en ello pudieron intervenir condicionantes y presiones de índole ajena a la técnica.
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- 2022
13. Historical Interrelationship of Railways and Cities from an Urban Viewpoint.
- Author
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Santos y Ganges, Luis, primary
- Published
- 2023
- Full Text
- View/download PDF
14. The Historical Interrelationship of Railways and Cities from an Urban Viewpoint.
- Author
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Santos y Ganges, Luis, Ediciones Universidad de Valladolid, Santos y Ganges, Luis, and Ediciones Universidad de Valladolid
- Abstract
This paper offers a conceptual overview of the state of the art relating to the historical interrelationships between railways and cities from the point of view of town planning, covering the good century and a half that there have been railways in the Iberian Peninsula. It addresses both urban and railway history, reconsidering major issues in this relationship. These are: 1) the tracks of lines as they cross cities and the role they play in urban life, 2) the passenger buildings of stations, seen as a focus of urban centrality, 3) the developing complexity of cities and railways, together with the installation of new road and port infrastructures, and 4) the problems emerging from urban expansion in the final third of the twentieth century, mostly after the restoration of democracy, specifically the question of the limited permeability of rail tracks and the interpretation as a social barrier of the physical barrier thus constituted by the railway. An idea that railways are a problem grew up within this historical interrelationship.
- Published
- 2023
15. Los grupos de casas ultrabaratas de la obra sindical del hogar en los años 1940: Las barriadas del General Yagüe
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Santos y Ganges, Luis, Ediciones Universidad de Valladolid, Santos y Ganges, Luis, and Ediciones Universidad de Valladolid
- Abstract
El libro trata sobre las "casas ultrabaratas", un fenómeno histórico-urbanístico de la política de vivienda durante el primer franquismo, a cargo de una entidad del Movimiento, la Obra Sindical del Hogar. Y en concreto se analizan los grupos de vivienda que el general Juan Yagüe Blanco impulsó en Burgos, Miranda, Soria y Logroño. Se trató de barriadas periféricas de vivienda protegida proyectadas en los años 1940 para una pequeña parte de los habitantes de infravivienda, suponiendo para sus beneficiarios el abandono de cuevas, chabolas y tugurios aun a costa de tener que vivir muy lejos de la ciudad, significando para el Movimiento un logro de la "justicia social" que pregonaban (de ahí que se convirtieran en elementos de propaganda contra una realidad de insuficiencia e injusticia flagrantes), y, en definitiva, conformando un testimonio de una ideología, de unas limitaciones, contradicciones e improvisaciones, y de unos modos de hacer de la política de vivienda del primer franquismo. Así, los grupos ultraperiféricos de casitas ultrabaratas semirrurales en planta baja sobre parcelas de más de trescientos metros cuadrados fueron una manifestación insólita, pero de algún modo significativa, de la vivienda social española del siglo pasado., En conformidad con las condiciones del contrato de edición de "Ediciones Universidad de Valladolid", esta monografía se publicará inicialmente en papel. Transcurrido un periodo de embargo, se difundirá en formato digital y en acceso abierto.
- Published
- 2023
16. Archaeointensity record of weak field recurrence in Japan: new data from Late Yayoi and Kofun ceramic artifacts
- Author
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Tema, E, primary, Santos, Y, additional, Trindade, R, additional, Hartmann, G A, additional, Hatakeyama, T, additional, Terra-Nova, F, additional, Matsumoto, N, additional, Mitsumoto, J, additional, and Gulmini, M, additional
- Published
- 2022
- Full Text
- View/download PDF
17. Transportation and Urban Planning Under State Socialism
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Elvira Khairullina and Luis Santos y Ganges
- Published
- 2022
18. Patterns of fish distribution in tropical rock pools at Príncipe Island, Gulf of Guinea
- Author
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Azevedo e Silva, J, primary, Almeida, AJ, additional, Cravo, M, additional, Pais, MP, additional, Santos, Y, additional, and Paula, J, additional
- Published
- 2022
- Full Text
- View/download PDF
19. Application on infrared spectroscopy for the analysis of total phenolic compounds in fruits
- Author
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Santos, Y. J. S., primary, Malegori, C., additional, Colnago, L. A., additional, and Vanin, F. M., additional
- Published
- 2022
- Full Text
- View/download PDF
20. TUMOUR GRADE REGRESSION (TRG) AND CORRELATION WITH MAGNETIC RESONANCE IMAGING (MRI) IN RECTAL CANCER
- Author
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A, Ocanto, primary, Santos Y, Dos, additional, R, Cantero, additional, I, Rodríguez, additional, and R, Morera, additional
- Published
- 2022
- Full Text
- View/download PDF
21. Archaeointensity record of weak field recurrence in Japan: new data from Late Yayoi and Kofun ceramic artefacts.
- Author
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Tema, E, Santos, Y, Trindade, R, Hartmann, G A, Hatakeyama, T, Terra-Nova, F, Matsumoto, N, Mitsumoto, J, and Gulmini, M
- Subjects
- *
GEOMAGNETISM , *THERMOREMANENT magnetization , *CERAMICS , *ARCHAEOLOGICAL excavations , *DEMAGNETIZATION , *REMANENCE - Abstract
We present new absolute archaeointensity data from six archaeological sites situated in the Okayama Prefecture, Japan. The materials studied are well-dated fragments from pottery, ceramic coffins and haniwa artefacts. Their ages range from 160 AD to 675 AD, covering the Late Yayoi and Kofun periods. Rock magnetic experiments suggest the presence of magnetite and/or Ti-magnetite as the main carrier of the remanence, with a possible minor contribution of higher coercivity minerals. After thermal demagnetization experiments, the most magnetically stable samples were selected for archaeointensity analysis performed following the double-heating method proposed by Thellier and modified by Coe. Partial thermoremanent magnetization (pTRM) checks and pTRM tail-checks were performed for monitoring possible chemical alterations during heating. All measurements were corrected for both anisotropy and cooling-rate effects. Successful archaeointensity determinations, following rigorous selection criteria, were obtained for samples from all the investigated archaeological sites. Compared with literature data from Japan, the new high-quality data show significantly lower intensity values. They also reveal possible fast secular variation changes during the Late Yayoi period and very weak geomagnetic intensity field around 630 AD. Such values offer evidence of a possible recurrence of weak intensity field in East Asia, suggesting an ancient recurrence of the West Pacific Anomaly. The new data might change the archaeomagnetic field models interpretations in the area, even though more data are still necessary to better understand the secular variation in Japan and the temporal evolution of the geomagnetic field's behaviour in East Asia. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
22. La selección de los polos industriales del plan de desarrollo de 1964: el ejemplo del Valle del Duero
- Author
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Santos y Ganges, Luis, primary and Fernández-Maroto, Miguel, additional
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- 2022
- Full Text
- View/download PDF
23. VANISHING LUNG SYNDROME: A RARE PRESENTATION OF A YOUNG MALE WITH GIANT BULLOUS EMPHYSEMA
- Author
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LAGROTTA, G., primary, PATEL, K., additional, SANTOS, Y., additional, IGUINA, M., additional, and HECTOR, V., additional
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- 2022
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24. Las torres del telégrafo óptico español. Antecedentes y variantes del tipo arquitectónico
- Author
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Santos y Ganges, Luis and Lalana-Encinas, Laura
- Subjects
Spain ,Telégrafo óptico ,España ,Telegraphic tower ,Military architecture ,Torre telegráfica ,Arquitectura militar ,Torre aspillerada ,Optical telegraph ,Embrasure tower - Abstract
[EN] The Spanish optical telegraph was planned by the Ministry of the Interior in 1844 for civilian governmental use. It was a tower and telescope telegraph developed by Colonel José María Mathé, with a design clearly inspired by military sensibilities and considerations. This paper first analyses the contemporary military reference point of the embrasure tower as a military stronghold campaign. It goes on to examine the historical antecedents of watchtowers or signal towers from the 16th century and the freestanding or outpost towers from the 17th century. This is followed by a description of the architectural type of telegraph tower and an analysis of its variants, which gradually differed from the model typology in both form and materiality. Finally, it examines the military telegraph towers of the second and third Carlist wars. Thus, the historical and typological study emphasizes the military inspiration of the optical telegraph towers that has often gone unremarked in order to optimize the heritage characterization of the remaining structures., [ES] El telégrafo óptico español de uso civil gubernamental, planificado por el Ministerio de la Gobernación en 1844, fue un telégrafo de torre y anteojo, con un diseño-tipo de la torre de clara inspiración militar, a cargo del coronel José María Mathé. El artículo analiza en primer término el referente militar coetáneo de la torre aspillerada a modo de reducto de campaña. Posteriormente, se acomete el estudio de los antecedentes históricos en las torres vigía o de señales desde el siglo XVI y en las torres exentas o de avanzada desde el siglo XVII. Más adelante se expone el tipo arquitectónico de la torre telegráfica y se analizan sus variantes, que fueron diferenciándose de la tipología modelo tanto en su forma como en su materialidad. Finalmente, se hace un análisis de las torres telegráficas militares de la segunda y tercera guerra carlista. Este estudio histórico y tipológico enfatiza la inspiración militar de las torres de telegrafía óptica para la óptima caracterización patrimonial de los restos existentes., Este trabajo forma parte del Proyecto de Investigación titulado “Análisis y definición de estrategias para la caracterización, recuperación y puesta en valor del patrimonio de la obra pública. Una aproximación desde la escala territorial,” código PID2019-105877RA-I00, del Programa Estatal de I+D+i orientada a los retos de la sociedad, parte del Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020, de la Agencia Estatal de Investigación, Ministerio de Ciencia, Innovación y Universidades.
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- 2022
25. The towers of the Spanish optical telegraph. Antecedents and variants of the architectural type
- Author
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Santos y Ganges, Luis, primary and Lalana-Encinas, Laura, additional
- Published
- 2022
- Full Text
- View/download PDF
26. JOGOS AFRICANOS COMO PROPOSTA METODOLÓGICA NO ENSINO FUNDAMENTAL I: PERSPECTIVA INCLUSIVA E EMANCIPATÓRIA A PARTIR DA ETNOMATEMÁTICA
- Author
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Silva, E. A., primary, Drago, R., additional, Júnior, P. J. S., additional, and Santos, Y. R., additional
- Published
- 2022
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27. The Sottoborgo and the Capilla-escuela: The Services of the Planned Dispersed Settlement of the 20th Century in Italy, Portugal and Spain
- Author
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Basiricò, Tiziana, Braz Afonso, Rui, and Santos y Ganges, Luis
- Subjects
Capilla-escuela ,Dispersed settlement ,Colonización agraria ,Servicios rurales ,Sottoborgo ,Agrarian colonization ,Rural services ,Poblamiento disperso - Abstract
Las políticas de colonización agrícola de los países del sur de Europa en el siglo XX, han producido una transformación territorial relativamente intensa en determinadas zonas. Este proceso creó la necesidad de instalar a los agricultores en territorios sin estructuras de poblamiento. Se estudiaron y fueron desarrolladas algunas tipologías urbanas y arquitectónicas, creando borghi di servizi en Italia, colônias agrícolas en Portugal y pueblos de colonización en España. Frente al poblamiento esencialmente disperso del modelo italiano y portugués, en España se optó abrumadoramente por el poblamiento concentrado, si bien para aquellos espacios alejados de los pueblos se pensó en disponer, en algunos casos excepcionales, una aldea de servicios sumarios similar al sottoborgo italiano que fue denominada capilla-escuela. Para este tipo de construcción se utilizaron proyectos estándar, con pequeñas variaciones en la distribución de los edificios, repetidos en el territorio según el plan de parcelación., The agricultural colonization policies of southern European countries in the 20th century have produced a relatively intense territorial transformation in certain areas. This process created the need to install farmers in territories without settlement structures. Some urban and architectural typologies have been studied and developed, creating borghi di servizi in Italy, colônias agrícolas in Portugal and pueblos de colonización in Spain. Faced with the essentially dispersed settlement of the Italian and Portuguese model, in Spain it was overwhelmingly opted for concentrated settlement, although for those areas far from the pueblos de colonización it was thought to have, in some exceptional cases, a summary services village similar to the Italian sottoborgo that was called capilla-escuela. For this type of construction, standard projects were used, with small variations in the layout of the buildings, repeated in the territory according to the plan of parcelization., HUM813
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- 2022
28. Patterns of fish distribution in tropical rock pools at Príncipe Island, Gulf of Guinea
- Author
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Silva, J Azevedo e, Almeida, AJ, Cravo, M, Pais, MP, Santos, Y, and Paula, J
- Subjects
Behavioural ecology ,eastern tropical Atlantic ,fish assemblage ,habitat specificity ,intertidal zone ,rock pool characteristics ,rocky shores ,São Tomé and Príncipe ,Aquatic Science ,Ecology, Evolution, Behavior and Systematics - Abstract
Little is known about the ichthyofauna from intertidal rock pools of the west coast of Africa, especially in the Gulf of Guinea. Rock pools are characteristic habitats of the intertidal zone of structurally complex rocky shores, adding important niche space to coastal fish species. In this study, rock pools of three similar rocky shores of Príncipe Island were sampled to describe the composition, abundance and distribution of fish assemblages and their relation to parameters of pool structure (volume, depth), water mass (temperature, salinity and pH) and biology (algal and coral cover, and biological species richness). A total of 18 fish species and one leptocephalus larva of unknown species, representing 13 families, were observed during sampling. In decreasing order, the five-most-abundant species were the goby Bathygobius burtoni, night sergeant Abudefduf taurus, West African rockhopper Entomacrodus cadenati, sailfin blenny Microlipophrys velifer and Biafra doctorfish Prionurus biafraensis, which together represented 81% of the total number of fish recorded during this study. The four-most-abundant species also proved to be the better adapted to the range of conditions found in rock pools. Overall, larger rock pools with minimal biological cover and higher salinity were found to support higher fish abundance and species richness. Most species individually preferred rock pools with larger volumes, but some presented a degree of habitat specificity, such as the absence or presence of biological cover. Species that were more-active swimmers preferred deeper pools.
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- 2022
- Full Text
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29. Metodología de la investigación en la enseñanza-aprendizaje del español como segunda lengua (L2)/lengua extranjera (LE) SANTOS GARGALLO, Isabel y PASTOR CESTEROS, Susana (dirs.) Madrid: Arco/Libros, S.L., 2022, 1.ª edição, brochura, 391 pp. ISBN: 978-84-7
- Author
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Santos y Fernández, Mirta dos, primary
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- 2022
- Full Text
- View/download PDF
30. The Historical Interrelationship of Railways and Cities from an Urban Viewpoint. Conceptual review and application to the Iberian Peninsula.
- Author
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SANTOS Y GANGES, LUIS
- Subjects
CITIES & towns ,URBAN growth ,URBAN life ,URBAN planning ,PORT cities ,URBAN history ,PENINSULAS ,RAILROADS - Abstract
Copyright of TST - Transportes, Servicios y Telecomunicaciones is the property of Asociacion Iberica de Historia Ferroviaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
31. Ventral-temporal\parietal neural circuitry for holistic face- and object- perception: evidence from a case of prosopagnosia
- Author
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Bobes Leon, M.A., de Gelder, B., Castro Laguardia, A., Gongora, D., Santos, Y., Poyo Solanas, M., and Valdes Sosa, M.
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- 2023
- Full Text
- View/download PDF
32. METODOLOGÍA DE LA INVESTIGACIÓN EN LA ENSEÑANZA-APRENDIZAJE DEL ESPAÑOL COMO SEGUNDA LENGUA (L2)/LENGUA EXTRANJERA (LE).
- Author
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dos Santos y Fernández, Mirta
- Subjects
- *
GRADUATE education , *SPANISH language , *UNDERGRADUATE programs , *APPLIED linguistics , *KNOWLEDGE transfer , *SECOND language acquisition - Abstract
The article discusses the work "Metodología de la investigación en la enseñanza-aprendizaje del español como segunda lengua (L2)/lengua extranjera (LE)" directed by Isabel Santos Gargallo and Susana Pastor Cesteros. The work addresses research methodology in the field of teaching and learning Spanish as L2/LE, filling a gap in the Spanish bibliography. The book consists of 18 chapters that cover everything from research design to results and knowledge transfer. The chapters are written by experts with extensive experience in teaching Spanish as a second language or foreign language. The work is considered a reference for undergraduate and graduate programs in applied linguistics. [Extracted from the article]
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- 2022
33. DELETERIOUS EFFECT OF VICILINS FRACTIONS OF LEGUMINOUS SEEDS in vitro AND IN SEMI-FIELD CONDITIONS FOR Ceratitis capitata.
- Author
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MACEDO, C. S., FRANÇA, A. F. J., MACEDO, L. L. P., AMORIM, T. M. L., ARAÚJO, R. R. O., SANTOS, Y. Q., CARELLI, G. S. C., UCHOA, A. F., MACEDO, F. P., OLIVEIRA, A. S., SANTOS, E. A., and ARAUJO, J. N.
- Subjects
MEDITERRANEAN fruit-fly ,AFFINITY chromatography ,SEEDS ,COMMON bean ,BIOLOGICAL insecticides ,SEED proteins - Abstract
Copyright of HOLOS is the property of Instituto Federal do Rio Grande do norte - IFRN and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
34. Characterization of West Nile virus Koutango lineage from phlebotomine sandflies in Kenya.
- Author
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Jane Wambui Thiiru, Solomon Langat, Francis Mulwa, Stephanie Cinkovich, Hellen Koka, Santos Yalwala, Samoel Khamadi, Justus Onguso, Nicholas Odemba, Francis Ngere, Jaree Johnson, Timothy Egbo, Eric Garges, Elly Ojwang, and Fredrick Eyase
- Subjects
Medicine ,Science - Abstract
The West Nile virus (WNV), primarily transmitted by mosquitoes, is one of the most widespread flaviviruses globally, with past outbreaks occurring in the USA and Europe. Recent studies in parts of Africa, including Kenya, have identified the West Nile virus Koutango lineage (WN-KOUTV) among phlebotomine sandfly populations, however, our understanding of this virus remains limited. This study aimed to characterize WN-KOUTV from phlebotomine sandflies. Sandflies were sampled between 12th -16th March 2021 and 16th -20th March 2023 from six villages each in Baringo and Isiolo Counties, using CDC light traps. Female sandflies were taxonomically identified and pooled based on genus and site of collection. Virus isolation was performed in Vero cells. Viral genomes were determined using next-generation sequencing. Phylogenetic and molecular clock analyses were done to decipher the virus's evolutionary relationships. Comparative analyses of amino acid sequences were performed to determine variations. Protein modeling in Pymol was conducted to elucidate variations in key protein regions. Evolutionary pressure analysis investigated the selection pressures on the virus. In vitro experiments were done to investigate the virus growth kinetics in mammalian Vero E6 and mosquito C6/36 cells. We report the isolation of WN-KOUTV from Salabani in Baringo and Aremet in Isiolo, Kenya. The isolated WN-KOUTVs clustered with previously identified WN-KOUTV strains. Comparative analysis revealed a unique amino acid at NS5 653. The WN-KOUTV lineage as a whole is under purifying selective pressure, with diversifying pressure acting at site NS3 267. The current WN-KOUTV replicated in Vero E6 and C6/36 cells comparable to West Nile virus Lineage 1a, isolated from mosquitoes. Subsequent isolations of WN-KOUTV in phlebotomine sandflies suggest potential vectors, however, vector competence studies would confirm this. Replication in mammalian and insect cell lines suggests there may exist a vector/host relationship. We speculate the close genetic relationship of WN-KOUTV strains from East and West Africa may potentially be enabled by bird migratory routes between the two regions. If proven, this could point to a potential future pandemic pathway for this virus.
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- 2024
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35. Detection of pathogenic bacteria in ticks from Isiolo and Kwale counties of Kenya using metagenomics.
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Bryson Brian Kimemia, Lillian Musila, Solomon Langat, Erick Odoyo, Stephanie Cinkovich, David Abuom, Santos Yalwala, Samoel Khamadi, Jaree Johnson, Eric Garges, Elly Ojwang, and Fredrick Eyase
- Subjects
Medicine ,Science - Abstract
Ticks are arachnid ectoparasites that rank second only to mosquitoes in the transmission of human diseases including bacteria responsible for anaplasmosis, ehrlichiosis, spotted fevers, and Lyme disease among other febrile illnesses. Due to the paucity of data on bacteria transmitted by ticks in Kenya, this study undertook a bacterial metagenomic-based characterization of ticks collected from Isiolo, a semi-arid pastoralist County in Eastern Kenya, and Kwale, a coastal County with a monsoon climate in the southern Kenyan border with Tanzania. A total of 2,918 ticks belonging to 3 genera and 10 species were pooled and screened in this study. Tick identification was confirmed through the sequencing of the Cytochrome C Oxidase Subunit 1 (COI) gene. Bacterial 16S rRNA gene PCR amplicons obtained from the above samples were sequenced using the MinION (Oxford Nanopore Technologies) platform. The resulting reads were demultiplexed in Porechop, followed by trimming and filtering in Trimmomatic before clustering using Qiime2-VSearch. A SILVA database pretrained naïve Bayes classifier was used to classify the Operational Taxonomic Units (OTUs) taxonomically. The bacteria of clinical interest detected in pooled tick assays were as follows: Rickettsia spp. 59.43% of pools, Coxiella burnetii 37.88%, Proteus mirabilis 5.08%, Cutibacterium acnes 6.08%, and Corynebacterium ulcerans 2.43%. These bacteria are responsible for spotted fevers, query fever (Q-fever), urinary tract infections, skin and soft tissue infections, eye infections, and diphtheria-like infections in humans, respectively. P. mirabilis, C. acnes, and C. ulcerans were detected only in Isiolo. Additionally, COI sequences allowed for the identification of Rickettsia and Coxiella species to strain levels in some of the pools. Diversity analysis revealed that the tick genera had high levels of Alpha diversity but the differences between the microbiomes of the three tick genera studied were not significant. The detection of C. acnes, commonly associated with human skin flora suggests that the ticks may have contact with humans potentially exposing them to bacterial infections. The findings in this study highlight the need for further investigation into the viability of these bacteria and the competency of ticks to transmit them. Clinicians in these high-risk areas also need to be appraised for them to include Rickettsial diseases and Q-fever as part of their differential diagnosis.
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- 2024
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36. Emigration and public health: the sanitary question in the control of Portuguese emigrants, 1890s-1950s
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Santos Yvette
- Subjects
Social Sciences - Abstract
Human mobility represents a privileged field for analysing epidemic propagation, as well as identification and control practises at a world scale. Migrants are at the same time agents of disease dissemination and vulnerable persons highly exposed to epidemics in transit spaces such as city ports, ships, and settlement sites in the countries of origin. This paper aims to analyse how a country with a long and strong tradition in transatlantic and European migration, such as Portugal, incorporated concerns of Public Health in the policies and management of massive migration exits from the nineteenth century until the 1950s, particularly through the directives for the control and prevention of epidemics. In this way, we will analyse the intersection of two general state policies, epidemic control and migration surveillance.
- Published
- 2022
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37. EXCESS workshop: Descriptions of rising low-energy spectra
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P. Adari, A. Aguilar-Arevalo, D. Amidei, G. Angloher, E. Armengaud, C. Augier, L. Balogh, S. Banik, D. Baxter, C. Beaufort, G. Beaulieu, V. Belov, Y. Ben Gal, G. Benato, A. Benoît, A. Bento, L. Bergé, A. Bertolini, R. Bhattacharyya, J. Billard, I.M. Bloch, A. Botti, R. Breier, G. Bres, J-.L. Bret, A. Broniatowski, A. Brossard, C. Bucci, R. Bunker, M. Cababie, M. Calvo, P. Camus, G. Cancelo, L. Canonica, F. Cappella, L. Cardani, J.-F. Caron, N. Casali, G.del Castello, A. Cazes, R. Cerulli, B.A. Cervantes Vergara, D. Chaize, M. Chapellier, L. Chaplinsky, F. Charlieux, M. Chaudhuri, A.E. Chavarria, G. Chemin, R. Chen, H. Chen, F. Chierchie, I. Colantoni, J. Colas, J. Cooley, J.-M. Coquillat, E.C. Corcoran, S. Crawford, M. Crisler, A. Cruciani, P. Cushman, A. D'Addabbo, J.C. D'Olivo, A. Dastgheibi-Fard, M. De Jésus, Y. Deng, J.B. Dent, E.L. Depaoli, K. Dering, S. Dharani, S. Di Lorenzo, A. Drlica-Wagner, L. Dumoulin, D. Durnford, B. Dutta, L. Einfalt, A. Erb, A. Erhart, R. Essig, J. Estrada, E. Etzion, O. Exshaw, F. Favela-Perez, F. v. Feilitzsch, G. Fernandez Moroni, N. Ferreiro Iachellini, S. Ferriol, S. Fichtinger, E. Figueroa-Feliciano, J.-B. Filippini, D. Filosofov, J. A. Formaggio, M. Friedl, S. Fuard, D. Fuchs, A. Fuss, R. Gaïor, A. Garai, C. Garrah, J. Gascon , G. Gerbier, M. Ghaith, V.M. Ghete, D. Gift, I. Giomataris, G. Giroux, A. Giuliani, P. Gorel, P. Gorla, C. Goupy, J. Goupy, C. Goy, M. Gros, P. Gros, Y. Guardincerri, C. Guerin, V. Guidi, O. Guillaudin, S. Gupta, E. Guy, P. Harrington, D. Hauff, S. T. Heine, S. A. Hertel, S.E. Holland, Z. Hong, E.W. Hoppe, T.W. Hossbach, J.-C. Ianigro, V. Iyer, A. Jastram, M. Ješkovský, Y. Jin, J. Jochum, J. P. Johnston, A. Juillard, D. Karaivanov, V. Kashyap, I. Katsioulas, S. Kazarcev, M. Kaznacheeva, F. Kelly, B. Kilminster, A. Kinast, L. Klinkenberg, H. Kluck, P. Knights, Y. Korn, H. Kraus, B. von Krosigk, A. Kubik, N.A. Kurinsky, J. Lamblin, A. Langenkämper, S. Langrock, T. Lasserre, H. Lattaud, P. Lautridou, I. Lawson, S.J. Lee, M. Lee, A. Letessier-Selvon, D. Lhuillier, M. Li, Y.-T. Lin, A. Lubashevskiy, R. Mahapatra, S. Maludze, M. Mancuso, I. Manthos, L. Marini, S. Marnieros, R.D. Martin, A. Matalon, J. Matthews, B. Mauri, D. W. Mayer, A. Mazzolari, E. Mazzucato, H. Meyer zu Theenhausen, E. Michielin, J. Minet, N. Mirabolfathi, K. v. Mirbach, D. Misiak, P. Mitra, J-.L. Mocellin, B. Mohanty, V. Mokina, J.-P. Mols, A. Monfardini, F. Mounier, S. Munagavalasa, J.-F. Muraz, X.-F. Navick, T. Neep, H. Neog, H. Neyrial, K. Nikolopoulos, A. Nilima, C. Nones, V. Novati, P. O'Brien, L. Oberauer, E. Olivieri, M. Olmi, A. Onillon, C. Oriol, A. Orly, J.L. Orrell, T. Ortmann, C.T. Overman, C. Pagliarone, V. Palušová, P. Pari, P. K. Patel, L. Pattavina, F. Petricca, A. Piers, H. D. Pinckney, M.-C. Piro, M. Platt, D. Poda, D. Ponomarev, W. Potzel, P. Povinec, F. Pröbst, P. Privitera, F. Pucci, K. Ramanathan, J.-S. Real, T. Redon, F. Reindl, R. Ren, A. Robert, J.Da Rocha, D. Rodrigues, R. Rogly, J. Rothe, N. Rowe, S. Rozov, I. Rozova, T. Saab, N. Saffold, T. Salagnac, J. Sander, V. Sanglard, D. Santos, Y. Sarkis, V. Savu, G. Savvidis, I. Savvidis, S. Schönert, K. Schäffner, N. Schermer, J. Schieck, B. Schmidt, D. Schmiedmayer, C. Schwertner, L. Scola, M. Settimo, Ye. Shevchik, V. Sibille, I. Sidelnik, A. Singal, R. Smida, M. Sofo Haro, T. Soldner, J. Stachurska, M. Stahlberg, L. Stefanazzi, L. Stodolsky, C. Strandhagen, R. Strauss, A. Stutz, R. Thomas, A. Thompson, J. Tiffenberg, C. Tomei, M. Traina, S. Uemura, I. Usherov, L. Vagneron, W. Van De Pontseele, F.A. Vazquez de Sola Fernandez, M. Vidal, M. Vignati, A.L. Virto, M. Vivier, T. Volansky, V. Wagner, F. Wagner, J. Walker, R. Ward, S.L. Watkins, A. Wex, M. Willers, M.J. Wilson, L. Winslow, E. Yakushev, T.-T. Yu, M. Zampaolo, A. Zaytsev, V. Zema, D. Zinatulina, A. Zolotarova
- Subjects
Physics ,QC1-999 - Abstract
Many low-threshold experiments observe sharply rising event rates of yet unknown origins below a few hundred eV, and larger than expected from known backgrounds. Due to the significant impact of this excess on the dark matter or neutrino sensitivity of these experiments, a collective effort has been started to share the knowledge about the individual observations. For this, the EXCESS Workshop was initiated. In its first iteration in June 2021, ten rare event search collaborations contributed to this initiative via talks and discussions. The contributing collaborations were CONNIE, CRESST, DAMIC, EDELWEISS, MINER, NEWS-G, NUCLEUS, RICOCHET, SENSEI and SuperCDMS. They presented data about their observed energy spectra and known backgrounds together with details about the respective measurements. In this paper, we summarize the presented information and give a comprehensive overview of the similarities and differences between the distinct measurements. The provided data is furthermore publicly available on the workshop’s data repository together with a plotting tool for visualization.
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- 2022
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38. Osteology of the Small-Sized Hyphessobrycon piabinhas Fowler 1941 (Characiformes, Characidae) With a Discussion on Developmentally Truncated Characters in the Family.
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Marinho MMF, De Santana Santos Y, and Da Silva JI
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- Animals, Characidae anatomy & histology, Characidae genetics, Biological Evolution, Osteology, Bone and Bones anatomy & histology, Phylogeny, Body Size
- Abstract
Evolutionary body size decrease has profound consequences for the morphology of an organism. In the evolution of the Characidae, the most species-rich family of Neotropical fishes, a prominent trend is the reduction of body size. The most typical effect is the simplification and reduction of morphological features through terminal deletion processes, resulting in the loss of skeletal elements and structures. To provide further information on the matter, we present a detailed description of the skeleton of Hyphessobrycon piabinhas, a poorly known, small representative of the largest genus of Characidae. We further discuss the identity and phylogenetic relationships of H. piabinhas. It belongs to the subfamily Stethaprioninae and exhibits considerable morphological similarity to other congeners from neighboring drainage systems. We identify several morphological simplifications in H. piabinhas and discuss them based on ontogenetic data available for Characiformes. These developmentally truncated elements are also present in many other small representatives of the family and seem to be among the first morphological modifications to occur in the context of body size reduction of Characidae. We argue that structural losses are not strictly correlated with sizes below 26 mm SL, although the most notable simplifications are typically observed in the miniatures., (© 2024 Wiley Periodicals LLC.)
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- 2024
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39. Provider perspectives on equity in use of mobile health autism screening tools.
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Zuckerman KE, Rivas Vazquez LA, Morales Santos Y, Fuchu P, Broder-Fingert S, Dolata JK, Bedrick S, Fernandez J, Fombonne E, and Sanders BW
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- Humans, Mass Screening methods, Attitude of Health Personnel, Primary Health Care, Male, Female, Child, Educational Personnel, Autism Spectrum Disorder, Adult, Autistic Disorder diagnosis, Telemedicine
- Abstract
Lay Abstract: Families may find information about autism online, and health care and education providers may use online tools to screen for autism. However, we do not know if online autism screening tools are easily used by families and providers. We interviewed primary care and educational providers, asking them to review results from online tools that screen for autism. Providers had concerns about how usable and accessible these tools are for diverse families and suggested changes to make tools easier to use., Competing Interests: Declaration of conflicting interestsThe author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: S.B.-F. is on the Scientific Advisory Board of EarliTec Diagnostics Inc. No other authors have conflicts of interest to disclose.
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- 2024
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40. Eleven years impact of a stepwise educational program on healthcare associated infections and antibiotics consumption in an intensive care unit of a tertiary hospital in Brazil.
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Giovanetti JN, Libera PHD, da Silva MLF, Boszczowski Í, Junior LCMC, de Albuquerque Pessoa Dos Santos Y, Forte DN, de Nardi R, Zigaib R, and Park M
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- Humans, Brazil epidemiology, Middle Aged, Male, Female, Prospective Studies, Adult, Aged, Urinary Tract Infections drug therapy, Incidence, Intensive Care Units, Anti-Bacterial Agents therapeutic use, Tertiary Care Centers, Cross Infection prevention & control, Cross Infection epidemiology
- Abstract
Background: Hospital acquired infections (HAI) and liberal use of broad-spectrum antibiotics are common in intensive care unit(ICU)s of low-middle income countries. We investigated the long-term association of a stepwise multifaceted educational program with the incidence of HAIs and antibiotics use in a Brazilian ICU. We also evaluated the program's cost impact., Methods: We retrieved data from a prospective daily collected database of a twelve bedrooms ICU, all admitted patients within a period of eleven years were enrolled., Findings: From 03/15/2007 to 09/11/2019, we admitted 3059 patients where 2406 (79%) survived the ICU stay. Median age was 51 years-old, and median SAPS3 was 53. The initial density of catheter related blood infection (4.3 events / 1000 patients-day), urinary tract infection (9.2 event / 1000 patients-day) and ventilator associated pneumonia (54.9 events / 1000 patients-day) felt during the observed period to (0.35 events / 1000 patients-day), (0 events / 1000 patients-day), and (1.5 events / 1000 patients-day) respectively. The days of antibiotic therapy also decreased from 797.9 days of therapy / 1000 patients day to 292.3 days of therapy / 1000 patients day. The total cost per patient also decreased. The adjusted mortality rate was steady during the studied period from 23.2% to 22.9%., Interpretation: A stepwise multifaceted educational program is an effective way to reduce hospital-associated infections, improve the rational use of antibiotics, and reduce costs. This impact occurred in a long term, and is probably consistent., Competing Interests: Declaration of competing interest None., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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41. A Case of Low-Grade Appendiceal Mucinous Neoplasm: The Role of Preoperative Imaging and Surgical Technique in Achieving Favorable Outcomes.
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Meza-Martinez DA, Suro Santos Y, Andrade-Ordoñez SJ, Palomino-Payan JA, and Fematt-Rodriguez BJ
- Abstract
Appendiceal mucinous neoplasms may present without symptoms or with chronic pain in the right lower quadrant. This report describes a case of a 35-year-old woman who presented with chronic right lower quadrant pain and was found to have a low-grade appendiceal mucinous neoplasm (LAMN). Physical examination revealed localized tenderness in the right lower quadrant with no additional symptoms. Preoperative laboratory results were normal, and a CT scan revealed a cystic appendiceal lesion with an internal calcification, initially mistaken for a fecalith, which led to the decision for exploratory laparotomy. Intraoperative findings confirmed the presence of a cystic-like appendiceal lesion, and an open appendectomy was performed. Histopathological examination revealed a low-grade appendiceal mucinous neoplasm (LAMN) confined to the mucosa, without evidence of metastatic disease. The patient had an uneventful recovery and required no additional treatment. This case highlights that surgical intervention with proper technique for confined appendiceal neoplasms, combined with effective preoperative imaging and thorough histopathological examination, is crucial for diagnosis and effective management, ensuring favorable outcomes., Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Meza-Martinez et al.)
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- 2024
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42. Lisinopril increases lung ACE2 levels and SARS-CoV-2 viral load and decreases inflammation but not disease severity in experimental COVID-19.
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Silva-Santos Y, Pagni RL, Gamon THM, de Azevedo MSP, Bielavsky M, Darido MLG, de Oliveira DBL, de Souza EE, Wrenger C, Durigon EL, Luvizotto MCR, Ackerman HC, Marinho CRF, Epiphanio S, and Carvalho LJM
- Abstract
COVID-19 causes more severe and frequently fatal disease in patients with pre-existing comorbidities such as hypertension and heart disease. SARS-CoV-2 virus enters host cells through the angiotensin-converting enzyme 2 (ACE2), which is fundamental in maintaining arterial pressure through the renin-angiotensin system (RAS). Hypertensive patients commonly use medications such as angiotensin-converting enzyme inhibitors (ACEi), which can modulate the expression of ACE2 and, therefore, potentially impact the susceptibility and severity of SARS-CoV-2 infection. Here we assessed whether treatment of ACE2-humanized (K18-hACE2) mice with the ACEi Lisinopril affects lung ACE2 levels and the outcome of experimental COVID-19. K18-hACE2 mice were treated for 21 days with Lisinopril 10 mg/kg and were then infected with 10
5 PFU of SARS-CoV-2 (Wuhan strain). Body weight, clinical score, respiratory function, survival, lung ACE2 levels, viral load, lung histology, and cytokine (IL-6, IL-33, and TNF-α) levels were assessed. Mice treated with Lisinopril for 21 days showed increased levels of ACE2 in the lungs. Infection with SARS-CoV-2 led to massive decrease in lung ACE2 levels at 3 days post-infection (dpi) in treated and untreated animals, but Lisinopril-treated mice showed a fast recovery (5dpi) of ACE2 levels. Higher ACE2 levels in Lisinopril-treated mice led to remarkably higher lung viral loads at 3 and 6/7dpi. Lisinopril-treated mice showed decreased levels of the pro-inflammatory cytokines IL-6 and TNF-α in the serum and lungs at 6/7dpi. Marginal improvements in body weight, clinical score and survival were observed in Lisinopril-treated mice. No differences between treated and untreated infected mice were observed in respiratory function and lung histology. Lisinopril treatment showed both deleterious (higher viral loads) and beneficial (anti-inflammatory and probably anti-constrictory and anti-coagulant) effects in experimental COVID-19. These effects seem to compensate each other, resulting in marginal beneficial effects in terms of outcome for Lisinopril-treated animals., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Silva-Santos, Pagni, Gamon, de Azevedo, Bielavsky, Darido, de Oliveira, de Souza, Wrenger, Durigon, Luvizotto, Ackerman, Marinho, Epiphanio and Carvalho.)- Published
- 2024
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43. Severe Combined Immunodeficiency from a Homozygous DNA Ligase 1 Mutant with Reduced Catalytic Activity but Increased Ligation Fidelity.
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Alajlan H, Raducanu VS, Lopez de Los Santos Y, Tehseen M, Alruwaili H, Al-Mazrou A, Mohammad R, Al-Alwan M, De Biasio A, Merzaban JS, Al-Mousa H, Hamdan SM, and Alazami AM
- Subjects
- Female, Humans, Male, Fibroblasts, Molecular Dynamics Simulation, Infant, DNA Ligase ATP genetics, DNA Ligase ATP metabolism, Homozygote, Mutation genetics, Severe Combined Immunodeficiency genetics
- Abstract
A cell's ability to survive and to evade cancer is contingent on its ability to retain genomic integrity, which can be seriously compromised when nucleic acid phosphodiester bonds are disrupted. DNA Ligase 1 (LIG1) plays a key role in genome maintenance by sealing single-stranded nicks that are produced during DNA replication and repair. Autosomal recessive mutations in a limited number of individuals have been previously described for this gene. Here we report a homozygous LIG1 mutation (p.A624T), affecting a universally conserved residue, in a patient presenting with leukopenia, neutropenia, lymphopenia, pan-hypogammaglobulinemia, and diminished in vitro response to mitogen stimulation. Patient fibroblasts expressed normal levels of LIG1 protein but exhibited impaired growth, poor viability, high baseline levels of gamma-H2AX foci, and an enhanced susceptibility to DNA-damaging agents. The mutation reduced LIG1 activity by lowering its affinity for magnesium 2.5-fold. Remarkably, it also increased LIG1 fidelity > 50-fold against 3' end 8-Oxoguanine mismatches, exhibiting a marked reduction in its ability to process such nicks. This is expected to yield increased ss- and dsDNA breaks. Molecular dynamic simulations, and Residue Interaction Network studies, predicted an allosteric effect for this mutation on the protein loops associated with the LIG1 high-fidelity magnesium, as well as on DNA binding within the adenylation domain. These dual alterations of suppressed activity and enhanced fidelity, arising from a single mutation, underscore the mechanistic picture of how a LIG1 defect can lead to severe immunological disease., (© 2024. The Author(s).)
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- 2024
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44. Using In Silico Molecular Docking to Explain Differences in Receptor Binding Behavior of HHC and THCV Isomers: Revealing New Binding Modes.
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Haghdoost M, López de Los Santos Y, Brunstetter M, Ferretti ML, Roberts M, and Bonn-Miller MO
- Abstract
Even slight structural differences between phytocannabinoid isomers are usually enough to cause a change in their biological properties. In this study, we used in vitro CB1 agonism/antagonism assays to compare the receptor binding functionality of THCV (tetrahydrocannabivarin) and HHC (hexahydrocannabinol) isomers and applied molecular docking to provide an explanation for the difference in the activities. No CB1 agonism was observed for ∆9- and ∆8-THCV. Instead, both isomers antagonized CP 55940, with ∆9-THCV being approximately two times more potent than the ∆8 counterpart (IC
50 = 52.4 nM and 119.6 nM for ∆9- and ∆8-THCV, respectively). Docking simulations found two binding poses for THCV isomers, one very similar to ∆9-THC and one newly discovered pose involving the occupation of side pocket 1 of the CB1 receptor by the alkyl chain of the ligand. We suggested the latter as a potential antagonist pose. In addition, our results established 9 R -HHC and 9 S -HHC among partial agonists of the CB1 receptor. The 9 R -HHC (EC50 = 53.4 nM) isomer was a significantly more potent agonist than 9 S (EC50 = 624.3 nM). ∆9-THC and 9 R -HHC showed comparable binding poses inside the receptor pocket, whereas 9 S -HHC adopted a new and different binding posture that can explain its weak agonist activity.- Published
- 2024
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45. Purine nucleosides replace cAMP in allosteric regulation of PKA in trypanosomatid pathogens.
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Ober VT, Githure GB, Volpato Santos Y, Becker S, Moya Munoz G, Basquin J, Schwede F, Lorentzen E, and Boshart M
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- Nucleosides pharmacology, Allosteric Regulation, Nucleotides, Cyclic, Guanosine, Adenosine, Purine Nucleosides, Cyclic AMP metabolism
- Abstract
Cyclic nucleotide binding domains (CNB) confer allosteric regulation by cAMP or cGMP to many signaling proteins, including PKA and PKG. PKA of phylogenetically distant Trypanosoma is the first exception as it is cyclic nucleotide-independent and responsive to nucleoside analogues (Bachmaier et al., 2019). Here, we show that natural nucleosides inosine, guanosine and adenosine are nanomolar affinity CNB ligands and activators of PKA orthologs of the important tropical pathogens Trypanosoma brucei , Trypanosoma cruzi, and Leishmania . The sequence and structural determinants of binding affinity, -specificity and kinase activation of PKAR were established by structure-activity relationship (SAR) analysis, co-crystal structures and mutagenesis. Substitution of two to three amino acids in the binding sites is sufficient for conversion of CNB domains from nucleoside to cyclic nucleotide specificity. In addition, a trypanosomatid-specific C-terminal helix (αD) is required for high affinity binding to CNB-B. The αD helix functions as a lid of the binding site that shields ligands from solvent. Selectivity of guanosine for CNB-B and of adenosine for CNB-A results in synergistic kinase activation at low nanomolar concentration. PKA pulldown from rapid lysis establishes guanosine as the predominant ligand in vivo in T. brucei bloodstream forms, whereas guanosine and adenosine seem to synergize in the procyclic developmental stage in the insect vector. We discuss the versatile use of CNB domains in evolution and recruitment of PKA for novel nucleoside-mediated signaling., Competing Interests: VO, GG, YV, SB, GM, JB, EL, MB No competing interests declared, FS CEO/CSO of BIOLOG Life Science Institute GmbH & Co. KG, (© 2023, Ober, Githure, Volpato Santos et al.)
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- 2024
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46. Multifunctional polymeric guanidine and hydantoin halamines with broad biocidal activity.
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Bromberg L, Magariños B, Torres BS, Santos Y, Concheiro A, Hatton TA, and Alvarez-Lorenzo C
- Subjects
- Humans, Guanidine, Polymers pharmacology, Biguanides pharmacology, Candida albicans, Hydantoins pharmacology, Methicillin-Resistant Staphylococcus aureus, Disinfectants pharmacology
- Abstract
Prolonged and excessive use of biocides during the coronavirus disease era calls for incorporating new antiviral polymers that enhance the surface design and functionality for existing and potential future pandemics. Herein, we investigated previously unexplored polyamines with nucleophilic biguanide, guanidine, and hydantoin groups that all can be halogenated leading to high contents of oxidizing halogen that enables enhancement of the biocidal activity. Primary amino groups can be used to attach poly(N-vinylguanidine) (PVG) and poly(allylamine-co-4-aminopyridine-co-5-(4-hydroxybenzylidene)hydantoin) (PAH) as well as a broad-spectrum commercial biocide poly(hexamethylene biguanide) (PHMB) onto a solid support. Halogenation of polymer suspensions was conducted through in situ generation of excess hypobromous acid (HBrO) from bromine and sodium hydroxide or by sodium hypochlorite in aqueous solutions, resulting in N-halamines with high contents of active > N-Br or > N-Cl groups. The virucidal activity of the polymers against human respiratory coronavirus HCoV-229E increased dramatically with their halogenation. Brominated PHMB-Br showed activation activity value > 5 even at 1 mg/L, and complete virus inhibition was observed with either PHMB-Br or PAH-Br at 10 mg/mL. Brominated PVG-Br and PAH-Br possessed fungicidal activity against C. albicans, while PHMB was fungistatic. PHMB, PHMB-Br and PAH polymers demonstrated excellent bactericidal activity against the methicillin-resistant S. aureus and vancomycin-resistant E. faecium. Brominated polymers (PHMB-Br, PVG-Br, PAH-Br) were not toxic to the HeLa monolayers, indicating acceptable biocompatibility to cultured human cells. With these features, the N-halamine polymers of the present study are a worthwhile addition to the arsenal of biocides and are promising candidates for development of non-leaching coatings., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
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- 2024
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47. Lower Limb Degloving Trauma Reconstruction: A Case Report.
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Sosa-Vazquez AA, Suro Santos Y, Serrato-Ruiz JA, Herrera-Valenzuela JA, Becerril-Pazara AA, and Hayakawa-Davila VC
- Abstract
Degloving injuries are caused by trauma with shear mechanisms, usually secondary to being run over in traffic accidents. It is characterized by the avulsion of skin and subcutaneous tissue, generating coverage deficit, and in severe cases, loss of the affected limb. Herein, we discuss a case of a six-year-old male run over by a truck, receiving a direct impact on the left lower extremity with a rubber tire. Debridement and surgical cleaning were performed and a negative pressure system was placed. Subsequently, dermal matrix and partial-thickness skin grafts were placed as coverage after the creation of a viable grafting bed., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2023, Sosa-Vazquez et al.)
- Published
- 2023
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48. Acute Abdomen Secondary to Ileal Diverticulum: A Case Report.
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Suro Santos Y, Fematt-Rodriguez BJ, Gonzalez-Ruiz JA, Fuentes-Hernandez JE, and Juarez-Garcia ML
- Abstract
Small-bowel diverticulosis is rare. We report the case of a male with an acute abdomen secondary to an ileal diverticulum. A 46-year-old male complained of progressive abdominal pain over 24 hours of evolution in the left flank. On physical examination, we found abdominal pain in the left flank and mesogastrium, tenderness, and signs of peritonitis. The simple abdominal CT showed a heterogeneous tubular image in the small bowel. We performed a diagnostic laparoscopy and found a normal cecal appendix. There was no free abdominal fluid or adhesions, and the colon was without diverticula. We found a single diverticulum of 4 cm in length and 2 cm in diameter in the small intestine and therefore converted the procedure to a laparotomy. We performed a bowel resection including the diverticulum and intestinal anastomosis. The patient reported remission of symptoms after surgery., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2023, Suro Santos et al.)
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- 2023
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49. No evidence for systemic low-grade inflammation in adult patients with early-treated phenylketonuria: The INGRAPH study.
- Author
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Giret C, Dos Santos Y, Blasco H, Paget C, Gonzalez L, Tressel N, Dieu M, Bigot A, Gissot V, Audemard-Verger A, and Maillot F
- Abstract
The question of an increased cardiovascular risk has been recently raised in adults with phenylketonuria (PKU). As low-grade systemic inflammation increases cardiovascular risk, the INGRAPH study aimed to evaluate low-grade inflammation in adult PKU patients compared to healthy controls and to determine the potential influence of Phe-controlled diet on inflammation. Twenty early-treated adult PKU patients, including a subgroup of 15 classical PKU patients, and 20 healthy volunteers were included. PKU patients and healthy subjects were matched on age, sex and body mass index class. Plasma concentrations of CRP, IFNg, IL1a, IL1b, IL2, IL6, IL10, and TNFα were measured in PKU patients and compared to controls. Plasma CRP was not different in the PKU group as compared to controls. No significant differences were observed between the two groups concerning plasma cytokines concentrations. Plasma CRP and cytokine profile were not different between "on diet" and "off diet" PKU patients. All these results were similar considering only the classical PKU subgroup. No differences were shown in plasma CRP and pro-inflammatory cytokines between adult PKU patients and healthy controls. Further studies are needed, including more patients and extensive characterization of systemic low-grade inflammation, as cardiovascular risk appears to be a new concern in adult PKU population., Competing Interests: The authors declare no conflicts of interest., (© 2023 The Authors. JIMD Reports published by John Wiley & Sons Ltd on behalf of SSIEM.)
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- 2023
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50. Biochemical and genetic biomarkers associated with nicotine dependence in Mexican smokers.
- Author
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Borrego-Soto G, Perez-Paramo YX, Hernández-Cabrera F, Alvarado-Monroy FM, Borrego G, Robles-Zamora A, Lazarus P, and Rojas-Martinez A
- Subjects
- Humans, Smokers, Genetic Markers, Oxides, Nicotine metabolism, Tobacco Use Disorder genetics
- Abstract
Cigarette smoking remains an important health concern and is still a leading cause of preventable mortality. Nicotine is the substance responsible for sustained tobacco use and dependence. Identification of biomarkers underlying nicotine dependence behavior is important to identify people at risk for this dependence. In the present study, we identified biochemical and genetic biomarkers of nicotine dependence detected by the Fagerström Test for Nicotine Dependence (FTDN) in Mexican smokers. The nicotine metabolites nicotine-N'-oxide, trans-3'-hydroxycotinine-glucuronide (3HC-O-Gluc), and nicotine-N-Gluc (Gluc) were useful to differentiate nicotine-dependent from non-dependent subjects (p < .0001) with an area under the curve (AUC) of 0.7818. Genetic variants in CYP2A6, FMO3, and UGT2B7 (rs2431413, rs28363545, and rs7439326, respectively) were associated with nicotine dependence (p = .03, p = .01, p = .01, respectively). Variations in the enzymatic activity of CYP2A6 were associated with altered nicotine-N'-oxide and 3HC-O-Gluc levels. Decreased urinary levels of 3HC-O-Gluc and increased nicotine-N'-oxide were associated with a decrease in the functional activity of CYP2A6. A strong positive correlation was observed between the ratio of urinary 3HC/cotinine, a measure of CYP2A6 activity, and the levels of 3HC-O-Gluc (p < .0001, r = .6835), while a strong negative correlation was observed with nicotine-N'-oxide (p < .0001, r = .6522) in nicotine-dependent subjects. No correlations were observed in non-nicotine-dependent subjects. These data suggest that particular urinary nicotine metabolites and genetic variants involved in nicotine metabolism are useful to identify subjects with nicotine dependence in the Mexican population., (© 2023 The Authors. Pharmacology Research & Perspectives published by British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics and John Wiley & Sons Ltd.)
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- 2023
- Full Text
- View/download PDF
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