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Your search keyword '"Sakurai, Yumiko"' showing total 16 results
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16 results on '"Sakurai, Yumiko"'

2. Multiplatform analyses reveal distinct drivers of systemic pathogenesis in adult versus pediatric severe acute COVID-19

Author

Druzak, Samuel, Iffrig, Elizabeth, Roberts, Blaine R., Zhang, Tiantian, Fibben, Kirby S., Sakurai, Yumiko, Verkerke, Hans P., Rostad, Christina A., Chahroudi, Ann, Schneider, Frank, Wong, Andrew Kam Ho, Roberts, Anne M., Chandler, Joshua D., Kim, Susan O., Mosunjac, Mario, Mosunjac, Marina, Geller, Rachel, Albizua, Igor, Stowell, Sean R., Arthur, Connie M., Anderson, Evan J., Ivanova, Anna A., Ahn, Jun, Liu, Xueyun, Maner-Smith, Kristal, Bowen, Thomas, Paiardini, Mirko, Bosinger, Steve E., Roback, John D., Kulpa, Deanna A., Silvestri, Guido, Lam, Wilbur A., Ortlund, Eric A., and Maier, Cheryl L.

Published
2023
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5. Dual biologics for severe asthma and atopic dermatitis: Synopsis of two cases and literature review

Author

Matsumoto, Takeshi, primary, Sakurai, Yumiko, additional, Tashima, Noriyuki, additional, Matoba, Tomoya, additional, Kaneko, Akiko, additional, Fujiki, Takahiro, additional, Kusakabe, Yusuke, additional, Nakayama, Emi, additional, Tanaka, Ayaka, additional, Tashima, Mayuko, additional, Yamamoto, Naoki, additional, and Aihara, Kensaku, additional

Published
2023
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12. Multiplatform Analyses Reveal Distinct Drivers of Systemic Pathogenesis in Adult Versus Pediatric COVID-19

Author

Druzak, Samuel, primary, Iffrig, Elizabeth, additional, Roberts, Blaine, additional, Zhang, Tiantian, additional, Roberts, Anne, additional, Sakurai, Yumiko, additional, Fibben, Kirby, additional, Chandler, Joshua, additional, Kim, Susan, additional, Schneider, Frank, additional, Mosunjac, Mario, additional, Mosunjac, Marina, additional, Geller, Rachel, additional, Wong, Andrew Kam Ho, additional, Paiardini, Mirko, additional, Bosinger, Steve, additional, Roback, John, additional, Stowell, Sean, additional, Arthur, Connie, additional, Anderson, Evan, additional, Rostad, Christina, additional, Chahroudi, Ann, additional, Ivanova, Anna, additional, Ahn, Jun, additional, Liu, Xueyun, additional, Maner-Smith, Kristal, additional, Bowen, Thomas, additional, Kulpa, Deanna, additional, Silvestri, Guido, additional, Lam, Wilbur A., additional, Ortlund, Eric, additional, and Maier, Cheryl, additional

Published
2022
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15. Membrane curvature and PS localize coagulation proteins to filopodia and retraction fibers of endothelial cells

Author

Carman, Christopher V., Nikova, Dessislava N., Sakurai, Yumiko, Shi, Jialan, Novakovic, Valerie A., Rasmussen, Jan T., Lam, Wilbur A., and Gilbert, Gary E.

Abstract

Prior reports indicate that the convex membrane curvature of phosphatidylserine (PS)-containing vesicles enhances formation of binding sites for factor Va and lactadherin. Yet, the relationship of convex curvature to localization of these proteins on cells remains unknown. We developed a membrane topology model, using phospholipid bilayers supported by nano-etched silica substrates, to further explore the relationship between curvature and localization of coagulation proteins. Ridge convexity corresponded to maximal curvature of physiologic membranes (radii of 10 or 30 nm) and the troughs had a variable concave curvature. The benchmark PS probe lactadherin exhibited strong differential binding to the ridges, on membranes with 4% to 15% PS. Factor Va, with a PS-binding motif homologous to lactadherin, also bound selectively to the ridges. Bound factor Va supported coincident binding of factor Xa, localizing prothrombinase complexes to the ridges. Endothelial cells responded to prothrombotic stressors and stimuli (staurosporine, tumor necrosis factor-α [TNF- α]) by retracting cell margins and forming filaments and filopodia. These had a high positive curvature similar to supported membrane ridges and selectively bound lactadherin. Likewise, the retraction filaments and filopodia bound factor Va and supported assembly of prothrombinase, whereas the cell body did not. The perfusion of plasma over TNF-α–stimulated endothelia in culture dishes and engineered 3-dimensional microvessels led to fibrin deposition at cell margins, inhibited by lactadherin, without clotting of bulk plasma. Our results indicate that stressed or stimulated endothelial cells support prothrombinase activity localized to convex topological features at cell margins. These findings may relate to perivascular fibrin deposition in sepsis and inflammation.

Published
2022
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16. Miniaturized Vascularized Bleeding Model of Hemostasis.

Author

Hardy ET, Sakurai Y, and Lam WA

Subjects
Hemorrhage, Hemostatics, Humans, Microfluidics, Hemostasis
Abstract

This chapter describes the development of a poly(dimethylsiloxane)-based microfluidic platform that is able to holistically assess and visualize the entire hemostatic process in vitro. The microfluidic platform includes (1) integration of intact endothelium, (2) physiological flow conditions, (3) controlled mechanical injury to study global hemostatic potential of a patient's whole blood samples in a microvascular model and for dissecting pathophysiologic mechanisms of diseases., (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2022
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