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Evaluating neighborhood performance is crucial for achieving long-term zero-carbon goals, enabling efficient energy, cost, and resource sharing among buildings. This task requires balancing multiple criteria and managing uncertainties, emphasizing the importance of performance robustness alongside high performance. This article introduces a flexible multi-criteria approach for evaluating neighborhood performance, focusing on greenhouse gas (GHG) emissions and energy use across different life cycle stages. Flytårnet, a Norwegian neighborhood with zero-emission ambitions, serves as a case study. The methodology incorporates the T-robust method, an established robustness-based approach, to select high-performance, resilient neighborhood designs under various uncertainties. Results indicate that when assessing buildings as key components and considering energy delivered during the operational phase, including photovoltaic generation, the supplied energy ranges from 25 to 80 kWh/m2/year. Over a 60-year period, life cycle GHG emissions span from 4 to 12 kg CO2-eq./m2/year, accounting for uncertainties and encompassing material production and replacement, as well as energy consumption and generation. However, the optimal design choice varies based on whether life cycle stages beyond the use stage are considered. This research provides valuable insights for decision-makers and designers seeking effective neighborhood designs in early-stage planning, considering diverse and conflicting performance criteria to achieve zero-emission goals.

In this paper, we report a comprehensive and consistent annotation of the locus encoding the β-chain of the equine T-cell receptor (TRB), as inferred from recent genome assembly using bioinformatics tools. The horse TRB locus spans approximately 1 Mb, making it the largest locus among the mammalian species studied to date, with a significantly higher number of genes related to extensive duplicative events. In the region, 136 TRBV (belonging to 29 subgroups), 2 TRBD, 13 TRBJ, and 2 TRBC genes, were identified. The general genomic organization resembles that of other mammals, with a V cluster of 135 TRBV genes located upstream of two in-tandem aligned TRBD-J-C clusters and an inverted TRBV gene at the 3′ end of the last TRBC gene. However, the horse b-chain repertoire would be affected by a high number of non-functional TRBV genes. Thus, we queried a transcriptomic dataset derived from splenic tissue of a healthy adult horse, using each TRBJ gene as a probe to analyze clonotypes encompassing the V(D)J junction. This analysis provided insights into the usage of the TRBV, TRBD, and TRBJ genes and the variability of the non-germline-encoded CDR3. Our results clearly demonstrated that the horse β-chain constitutes a complex level of variability, broadly like that described in other mammalian species.

Transposable elements (TEs) have been historically depicted as detrimental genetic entities that selfishly aim at perpetuating themselves, invading genomes, and destroying genes. Scientists often co-opt “special” TEs to develop new and powerful genetic tools, that will hopefully aid in changing the future of the human being. However, many TEs are gentle, rarely unleash themselves to harm the genome, and bashfully contribute to generating diversity and novelty in the genomes they have colonized, yet they offer the opportunity to develop new molecular tools. In this review we summarize 30 years of research focused on the Bari transposons. Bari is a “normal” transposon family that has colonized the genomes of several Drosophila species and introduced genomic novelties in the melanogaster species. We discuss how these results have contributed to advance the field of TE research and what future studies can still add to the current knowledge.

Responsive building envelopes (RBEs) are central to developing sustainability strategies for zero emission/energy buildings (ZEBs). RBEs are a large group of complex technologies and systems, which is why multi-criteria decision making (MCDM) methods are helpful to navigate sustainability assessments considering various performance indicators. This article first provides a literature review of assessment criteria and key performance indicators for RBEs and an analysis of existing robustness-based MCDM methods. Then, a methodological approach to assess RBE designs in ZEB projects is proposed as an extension of a novel robustness-based MCDM method that normalizes the objective functions according to defined targets and combines them into one comprehensive indicator (MT-KPI), thereby eliminating the need to weight objectives. The proposed methodological approach is finally tested on a case study of a Norwegian ZEB, where five competitive RBE designs (including building integrated photovoltaics, phase change material, and electrochromic windows) and eight occupancy and climate scenarios are investigated considering three main performance areas: energy use, thermal comfort, and load matching. The results in the case study show that with the proposed MCDM approach the different designs have MT-KPI values between 1.4 and 12.8, where a lower value is better. In this specific case, the most robust building RBE alternative was identified as the one with electrochromic windows and a control based on incident solar radiation and indoor air temperature.