111 results on '"Ramírez, I."'
Search Results
2. Acute and chronic mammary periprosthetic histological changes of the muscle
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Cámara-Pérez, J., Jimena, I., Rodríguez-Cano, M.A., Sanz-Zorrilla, A., Osuna-Soto, J., Sánchez-Ramírez, I., Sánchez-Medianero, T., Gálvez-Medina, M.J., Ortega-Salas, R., and Leiva-Cepas, F.
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- 2024
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3. Palliative care management in patients with Parkinson’s disease and other movement disorders in Spain. National survey of neurologists
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Álvarez Saúco, M., García- Ramos, R., Legarda Ramírez, I., Carrillo García, F., Fernández Bueno, J., Martí Martínez, S., González García, B., Moya-Martínez, A., and Santos-García, D.
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- 2024
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4. Manejo de la atención paliativa de los pacientes con enfermedad de Parkinson y otros trastornos del movimiento en España. Encuesta Nacional a neurólogos
- Author
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Álvarez Saúco, M., García- Ramos, R., Legarda Ramírez, I., Carrillo García, F., Fernández Bueno, J., Martí Martínez, S., González García, B., Moya-Martínez, A., and Santos-García, D.
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- 2024
- Full Text
- View/download PDF
5. Bimetallic systems of ZnO/Al/Ag applied on cell PEC and photocatalytic system
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Ortiz Rabell, G., Juárez-Ramírez, I., and Alfaro Cruz, M.R.
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- 2023
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6. Photochemistry of ZnO/GeO2 film for H2 production
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Ortiz Rabell, G., Alfaro Cruz, M.R., and Juárez-Ramírez, I.
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- 2022
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7. Photoelectrochemical (PEC) analysis of ZnO/Al photoelectrodes and its photocatalytic activity for hydrogen production
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Rabell, G. Ortiz, Alfaro Cruz, M.R., and Juárez-Ramírez, I.
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- 2022
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8. Catalytic hydrothermal liquefaction of microalgae cultivated in wastewater: Influence of ozone-air flotation on products, energy balance and carbon footprint
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Nava-Bravo, I., Velasquez-Orta, S.B., Monje-Ramírez, I., Güereca, L.P., Harvey, A.P., Cuevas-García, R., Yáñez-Noguez, I., and Orta-Ledesma, M.T.
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- 2021
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9. First insight into plastics ingestion by fish in the Gulf of California, Mexico
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Salazar-Pérez, C., Amezcua, F., Rosales-Valencia, A., Green, L., Pollorena-Melendrez, J.E., Sarmiento-Martínez, M.A., Tomita Ramírez, I., Gil-Manrique, B.D., Hernandez-Lozano, M.Y., Muro-Torres, V.M., Green-Ruiz, C., Piñon-Colin, T.D.J., Wakida, F.T., and Barletta, M.
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- 2021
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10. Seabird bycatch in European waters.
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Ramírez, I., Mitchell, D., Vulcano, A., Rouxel, Y., Marchowski, D., Almeida, A., Arcos, J. M., Cortes, V., Lange, G., Morkūnas, J., Oliveira, N., and Paiva, V. H.
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BYCATCHES , *NUMBERS of species , *MIGRATORY animals , *ACQUISITION of data , *FISHERIES - Abstract
The incidental capture (bycatch) of seabirds is a global conservation issue and a top threat to European species that demands urgent conservation and management action. Here, we present the first European review of seabird bycatch data, considering all fishing gears and data collection methods available in the region. We calculate seabird bycatch numbers per species, family, country and European marine region and assess the reliability of the data available. The cumulative bycatch estimate extracted from this review suggests that about 195,000 seabirds (ranging from around 130,000 to 380,000) are bycaught in European waters annually. The most affected seabird species is the Common Guillemot Uria aalge with over 31,000 birds killed per year. The marine region with the highest bycatch estimate is the Northeast Atlantic (over 115,000 seabirds year−1). Gillnet fisheries are responsible for the highest bycatch levels, with over 95,000 seabirds year−1, followed by longline fisheries. The families most affected by bycatch are Anatidae and Alcidae. These numbers are likely an underestimation since we were unable to find bycatch estimates, or to extrapolate estimates from available bycatch data for 12 (out of 34) European coastal states. Our assessment also identified significant data gaps in key areas such as Gran Sol (in the north‐east Atlantic), the central and Eastern Mediterranean and the Black Sea. Combining systematic data collection with immediate implementation of mitigation measures will be crucial to fill in knowledge gaps, reduce current mortality levels and meet international conservation commitments such as those of the European Union and the Convention on Migratory Species. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Host‐plant sex and phenology of Buddleja cordata Kunth interact to influence arthropod communities.
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González‐Ramírez, I., López‐Gómez, V., Cano‐Santana, Z., Romero Pérez, A., and Hernández Cumplido, J.
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DIMORPHISM in plants , *SOCIAL influence , *SEXUAL dimorphism , *PLANT phenology , *PHENOLOGY , *PLANT variation - Abstract
Intraspecific variation in plants is expected to have profound impacts on the arthropod communities associated with them. Because sexual dimorphism in plants is expected to provide consistent variation among individuals of the same species, researchers have often studied the effect it has on associated arthropods. Nevertheless, most studies have focused on the effect of sexual dimorphism in a single or a few herbivores, thus overlooking the potential effects on the whole arthropod community. Our main objective was to evaluate effects of Buddleja cordata's plant‐sex on its associated arthropod community. We surveyed 13 pairs of male and female plants every 2 months during a year (June 2010 to April 2011). Every sampling date, we measured plant traits (water content and leaf thickness), herbivory, and the arthropod community. We did not find differences in herbivory between plant sex or through time. However, we found differences in water content through time, with leaf water‐content matching the environmental seasonality. For arthropod richness, we found 68 morphospecies associated with female and 72 with male plants, from which 53 were shared by both sexes. We did not observe differences in morphospecies richness; however, we found sex‐associated differences in the diversity of all species and differences on the diversity of the most abundant species with an interesting temporal component. During peak flowering season, male plants showed higher values on both parameters, but during the peak fructification season female plants showed the higher values on both diversity parameters. Our research exemplifies the interaction between plant‐phenology and plant‐sex as drivers of arthropod communities' diversity, even when plant sexual‐dimorphism is inconspicuous, and highlighting the importance of accounting for seasonal variation. We stress the need of conducting more studies that test this time‐dependent framework in other dioecious systems, as it has the potential to reconcile previous contrasting observations reported in the literature. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Palliative care management in patients with Parkinson’s disease and other movementdisorders. National survey of neurologists
- Author
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Álvarez Saúco, M., primary, García- Ramos, R., additional, Legarda Ramírez, I., additional, Carrillo García, F., additional, Fernández Bueno, J., additional, Martí Martínez, S., additional, González García, B., additional, Moya-Martínez, A., additional, and Santos-García, D., additional
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- 2023
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13. Development of SnO2–ZnO thin films as a photocatalyst for obtaining alternative fuels through photocatalytic reactions
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Alfaro Cruz, M.R., primary, Saldaña-Ramírez, A., additional, Juárez-Ramírez, I., additional, and Torres-Martínez, Leticia M., additional
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- 2023
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14. Experiencia clínica en el manejo de defectos óseos postraumáticos de huesos largos tratados con la técnica de inducción de membrana.
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Quiroz-Williams, J., Gaytán-Fernández S., S., Barragan-Hervella, R. G., Morgan-Padrón, S., Rojas-Durán, O., Martínez-Asención JP., J. P., and Castillo-Ramírez, I. O.
- Abstract
Copyright of Acta Ortopédica Mexicana is the property of Sociedad Mexicana de Ortopedia, AC and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
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15. One-step microwave-assisted hydrothermal synthesis of α-Bi2Mo3O12 without surfactants at low temperature for their application in tetracycline photodegradation
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Rodríguez-Girón, J.S., primary, Hernández-Uresti, D.B., additional, Obregón, S., additional, Juárez-Ramírez, I., additional, and Sánchez-Martínez, D., additional
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- 2022
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16. Population-based survey on menopausal symptoms and treatment use
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Baquedano, L., primary, Coronado, P., additional, De la Viuda, E., additional, Sánchez, S., additional, Otero, B., additional, Ramírez, I., additional, Llaneza, P., additional, and Mendoza, N., additional
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- 2022
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17. Synthesis, characterization, and photocatalytic CO2 reduction evaluation of the CaWxMo1−xO4 (x = 0–1) solid solution.
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Garay-Rodríguez, Luis F., Ibarra-Rodríguez, Luz I., Torres-Martínez, Leticia M., and Juárez-Ramírez, I.
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SOLID solutions ,PHOTOREDUCTION ,CARBON dioxide reduction ,CARBON dioxide in water ,CARBON monoxide - Abstract
A solid solution with the formula CaW
x Mo1−x O4 (x = 0–1) was synthesized using a microwave-assisted hydrothermal method. The effect of W6+ concentration was correlated with the solid solution's physicochemical properties. All the samples were characterized to analyze their structural, morphological, optical, and electrochemical properties, corroborating the solid solution formation and the changes caused by differences in W/Mo compositions. The samples grew in different morphologies, varying based on their composition and slightly reducing their bandgap. All solid solution compositions exhibited activity in simultaneous photocatalytic water splitting and carbon dioxide reduction reactions under UV irradiation without a cocatalyst or sacrificial agent. Various products were evolved, with carbon monoxide being the most abundant product generated in most of the samples. The largest amount of carbon monoxide (6.7 μmol) was obtained in the sample with the formula CaW0.8 Mo0.2 O4 , and its superior performance can be attributed to the larger distortion in the cell due to the introduction of W6+ in higher concentrations. [ABSTRACT FROM AUTHOR]- Published
- 2023
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18. Computer vision tools for the automatic evaluation of collagen VI deficiencies
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Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions, Vilaplana Besler, Verónica, Porta Pleite, Josep Maria, Ramírez i Márquez, Alex, Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions, Vilaplana Besler, Verónica, Porta Pleite, Josep Maria, and Ramírez i Márquez, Alex
- Abstract
Deficiencies in the structure of collagen VI are a common cause of neuromuscular diseases. Such diseases typically require assisted ventilation and result in a severely reduced life expectancy. Collagen VI structural defects are related to mutations of three main genes. Currently the CRISPR technology offers a possibility to correct the wrong genes. However, the regulatory agencies would not approve any treatment without an objective methodology to evaluate its effectiveness. This project aims at providing a computer vision solution to evaluate the state of patients with collagen VI deficiencies. The idea is to provide objective metrics of the patient state from images of muscular tissue obtained with a confocal microscope. Currently some tools are available to this end, but only for low resolution 2D images. This project proposes to extend this previous work to the analysis of high-resolution 3D stacks of images. The project involves the development of classical computer vision tools to derive relevant features from the stacks of images and the use of classification tools to generate an overall evaluation of each patient. This analysis will be complemented with the development of a solution based on the use of a convolutional neural network. To this end, data augmentation techniques will be of primary importance since collagen VI-related problems are rare diseases and, thus, there is a severe lack of training data.
- Published
- 2022
19. Population-based survey on menopausal symptoms and treatment use.
- Author
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Baquedano, L., Coronado, P., De la Viuda, E., Sánchez, S., Otero, B., Ramírez, I., Llaneza, P., and Mendoza, N.
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GENITOURINARY diseases ,HORMONE therapy for menopause ,HOT flashes ,POSTMENOPAUSE ,PERIMENOPAUSE ,SYMPTOMS - Abstract
Copyright of Climacteric is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
20. Computer vision tools for the automatic evaluation of collagen VI deficiencies
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Ramírez i Márquez, Alex, Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions, Vilaplana Besler, Verónica, and Porta Pleite, Josep Maria
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Deep learning (Machine learning) ,Computer Vision ,Convolutional Neural Networks ,Visió per ordinador ,Collagen VI ,Computer-Aided Diagnosis ,Neural networks (Computer science) ,Neuromuscular diseases ,Deep Learning ,Xarxes neuronals (Informàtica) ,Informàtica::Intel·ligència artificial [Àrees temàtiques de la UPC] ,Collagen ,Col·lagen ,Aprenentatge profund - Abstract
Deficiencies in the structure of collagen VI are a common cause of neuromuscular diseases. Such diseases typically require assisted ventilation and result in a severely reduced life expectancy. Collagen VI structural defects are related to mutations of three main genes. Currently the CRISPR technology offers a possibility to correct the wrong genes. However, the regulatory agencies would not approve any treatment without an objective methodology to evaluate its effectiveness. This project aims at providing a computer vision solution to evaluate the state of patients with collagen VI deficiencies. The idea is to provide objective metrics of the patient state from images of muscular tissue obtained with a confocal microscope. Currently some tools are available to this end, but only for low resolution 2D images. This project proposes to extend this previous work to the analysis of high-resolution 3D stacks of images. The project involves the development of classical computer vision tools to derive relevant features from the stacks of images and the use of classification tools to generate an overall evaluation of each patient. This analysis will be complemented with the development of a solution based on the use of a convolutional neural network. To this end, data augmentation techniques will be of primary importance since collagen VI-related problems are rare diseases and, thus, there is a severe lack of training data.
- Published
- 2022
21. Development of Sno2-Zno Thin Films as a Photocatalyst for Obtaining Alternative Fuels Through Photocatalytic Reactions
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Alfaro, Maria, primary, Saldaña-Ramírez, A., additional, Juárez-Ramírez, I., additional, and Torres-Martínez, Leticia M., additional
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- 2022
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22. Manejo de la atención paliativa de los pacientes con enfermedad de Parkinson y otros trastornos del movimiento en España. Encuesta Nacional a neurólogos
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Álvarez Saúco, M., primary, García- Ramos, R., additional, Legarda Ramírez, I., additional, Carrillo García, F., additional, Fernández Bueno, J., additional, Martí Martínez, S., additional, González García, B., additional, Moya-Martínez, A., additional, and Santos-García, D., additional
- Published
- 2021
- Full Text
- View/download PDF
23. Hydrogen production of ZnO and ZnO/Ag films by photocatalysis and photoelectrocatalysis
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Rabell, G. Ortiz, primary, Cruz, M.R. Alfaro, additional, and Juárez-Ramírez, I., additional
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- 2021
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24. Caracterización de cepas de Escherichia coli uropatógena aisladas del tracto urinario de mujeres durante el embarazo
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García Cervantes, P. C., primary, Castañeda Meléndrez, A. M., additional, Osuna Ramírez, I., additional, and Bernal Reynaga, R., additional
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- 2021
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25. The Impact of Homestyles-2 Nutrition Education Curriculum on Family Mealtime Behaviors, Supports, and Perceptions of Parents/Caregivers of Children Ages 6 to 11
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Zeldman, J., Ramirez, I., Chambers, K., Brunson, L., Li, Y., O'Neal, L., Olfert, M., Byrd-Bredbenner, C., and Shelnutt, K.
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- 2023
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26. Evaluating Participant Recipe Changes in a Healthy Meal Kit Intervention for Families with Low Income
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McNulty, E., Chambers, K., Zambrano, R., Ramirez, I., Mitchell, A., House, L., Mathews, A., and Shelnutt, K.
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- 2023
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27. Influence of the Calcination Temperature on the Crystallographic, Compositional and Morphological Properties of Natural Hydroxyapatite Obtained from Sheep Bones.
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Restrepo-Ramírez, V., Arellano-Ramírez, I. D., Alzate-Acevedo, N., Restrepo-Parra, E., and Acosta-Medina, C. D.
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HYDROXYAPATITE , *FOURIER transform infrared spectroscopy , *SHEEP , *SCANNING electron microscopy , *DIFFRACTION patterns - Abstract
In this article, the study of the influence of the calcination temperature on the crystallographic, compositional, and morphological properties of the natural hydroxyapatite taken from sheep bones was carried out. The obtained samples were characterized by thermogravimetric analysis (TGA), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy, with elemental analysis (SEM-EDS) in order to obtain structural information, compositional and morphological properties. In the thermogravimetric curves, the decomposition temperatures of the organic phases present in the sheep bones powder were identified. In the X-ray diffraction analyzes, it was found that the diffraction patterns presented characteristic peaks of hydroxyapatite and, it was observed that the increase in temperature favors the growth crystallite size. Likewise, in the morphological analysis, changes in the samples were verified, finding greater agglomeration of the particles, while the EDS analyzes revealed the different Ca/P relationships for the calcination temperatures used. In the FTIR spectra, the characteristic vibrational bands of the phosphate and hydroxyl functional groups present in the samples were observed. Moreover, due to the temperature changes, the vibrational bands corresponding to water and proteins disappeared, and a decrease in the carbonate bands was also identified. [ABSTRACT FROM AUTHOR]
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- 2021
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28. Cuidados paliativos en enfermedad de Parkinson y otros trastornos del movimiento. Recomendaciones y protocolo de un grupo multidisciplinar de expertos
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Álvarez Saúco, M., Legarda Ramírez, I., Martí Martínez, S., Carrillo García, F., González García, B., Fernández Bueno, J., García-Ramos, R., and Santos-García, D.
- Abstract
Uno de los retos actuales en la enfermedad de Parkinson (EP) y otros trastornos del movimiento (TM) consiste en cómo y cuándo aplicar la atención paliativa. Conocedores de la escasa formación e implementación de este tipo de abordajes planteamos unas recomendaciones consensuadas de los cuidados paliativos (CP) con el fin de mejorar la calidad de vida de los pacientes y su entorno.
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- 2024
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29. Linking climate change vulnerability research and evidence on conservation action effectiveness to safeguard European seabird populations
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Henry Hakkinen, Silviu O. Petrovan, William J. Sutherland, Maria P. Dias, Eric I. Ameca, Steffen Oppel, Iván Ramírez, Becki Lawson, Aleksi Lehikoinen, Katharine M. Bowgen, Nigel G. Taylor, Nathalie Pettorelli, Hakkinen, H [0000-0003-1404-5798], Petrovan, SO [0000-0002-3984-2403], Sutherland, WJ [0000-0002-6498-0437], Dias, MP [0000-0002-7281-4391], Ameca, EI [0000-0001-8699-4090], Oppel, S [0000-0002-8220-3789], Ramírez, I [0000-0001-7944-8710], Lawson, B [0000-0003-1706-7140], Lehikoinen, A [0000-0002-1989-277X], Bowgen, KM [0000-0002-2414-8021], Taylor, N [0000-0002-8643-826X], Pettorelli, N [0000-0002-1594-6208], Apollo - University of Cambridge Repository, Repositório da Universidade de Lisboa, Finnish Museum of Natural History, Faculty Common Matters (Faculty of Biology and Environmental Sciences), Zoology, and Helsinki Institute of Sustainability Science (HELSUS)
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climate change ,Ecology ,conservation evidence ,1181 Ecology, evolutionary biology ,management interventions ,Western Europe ,pressure-state-response framework ,wildlife management ,sense organs ,conservation practice ,1172 Environmental sciences ,seabirds - Abstract
Publisher Copyright: © 2022 British Ecological Society. An increasing number of species are facing unprecedented levels of threat to their long-term survival due to the direct and indirect impacts of climate change. Key opportunities for science to inform wildlife management are linked to increasing our understanding of how changes in climatic conditions will impact species, as well as whether, and how, managers may facilitate species' ability to adapt to change. However, information on species' climate change vulnerability and the effectiveness of potential conservation actions are not yet strategically collected or collated; this disconnect between threat level, ecological research and conservation practice is reducing the opportunities to guide decision-making, ultimately hindering conservation outcomes. To demonstrate this point, we explore how existing knowledge can be brought together in a pressure-state-response framework that connects climate change ecology, conservation evidence assessments and management. Seabirds in Western Europe are used as a case study, as they are well-researched and vulnerable to climate change. Using a combination of literature reviews and surveys, we identify the main threats posed to seabirds in the region by climate change, as well as existing conservation actions that could be applied to lessen the impacts of each of these threats. Our results show that 29% of the types of actions considered for reducing the impacts of climate change on seabirds are either associated with conflicting evidence or lack sufficient information to make robust conclusions about their effectiveness: actions aiming at restoring or creating habitat, encouraging relocation, treating or preventing disease, and reducing inter-species competition all have limited or mixed evidence to support their use. Moreover, several threats identified by conservation practitioners as being of high priority to address, such as changes in prey abundance and eutrophication, have few or no viable identified actions to reduce their impact on seabirds. Synthesis and applications. We suggest that existing knowledge on species vulnerability to climate change and evidence of conservation action effectiveness should be more commonly brought together in tailored pressure-state-response frameworks. Such an approach provides an easily transferable platform for identifying missing information and areas where connections between research and management need to be tightened to improve conservation outcomes.
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- 2022
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30. High removal of PS and PET microplastics from tap water by using Fe 2 O 3 porous microparticles and photothermal irradiation with NIR light.
- Author
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Sanchez JM, Oliva J, Gomez-Solis C, Puentes-Prado E, Montes E, Juárez-Ramírez I, Garcia CR, and Moreno Palmerin J
- Abstract
This work reports the synthesis of Fe
2 O3 (FeO) microparticles using the Pechini method and their use to remove microplastics from tap water. The analysis by electronic microscopy revealed that the FeO microparticles (FeMicroPs) have a porous structure and are formed by interconnected grains with sizes of 80-120 nm. In addition, the X-ray diffraction analysis pointed out that the FeMicroPs are composed of γ- Fe2 O3 and α- Fe2 O3 phases. To remove the PS and PET microplastics with sizes of 0.1-3 μm from the tap water, FeO was added to the contaminated water and the mixture of FeO+microplastics was irradiated with focused NIR light (980 nm). This provoked the melting of the microplastics on the FeO surface. Later, the FeMicroPs with adsorbed microplastics was recovered with magnets. This last procedure permitted a high removal of microplastics from the tap water, and the adsorption capacity was 1000 mg/g. In the next step, the microplastics adsorbed on the FeO were irradiated with NIR light to induce its thermal decomposition by photothermal irradiation, this in turn, produced the elimination of the microplastics from the FeO surface and allowed its reuse to remove more microplastics from the tap water. The elimination of the microplastics from the FeO surface was confirmed by the FTIR and Raman techniques, since the vibrational peaks associated with the microplastics disappeared from the FeO surface after the photothermal irradiation. Thus, the results of this investigation suggest that the photothermal irradiation with NIR light not only facilitates the removal of microplastics from the tap water, but also, it was useful to degrade the microplastics definitively without producing more contamination. This technique could be used to remove microplastics in water treatment plants., Competing Interests: Declaration of Competing Interest X The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. ☐The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:, (Copyright © 2024. Published by Elsevier Ltd.)- Published
- 2024
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31. Animal life in the shallow subseafloor crust at deep-sea hydrothermal vents.
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Bright M, Gollner S, de Oliveira AL, Espada-Hinojosa S, Fulford A, Hughes IV, Hourdez S, Karthäuser C, Kolar I, Krause N, Le Layec V, Makovec T, Messora A, Mitchell J, Pröts P, Rodríguez-Ramírez I, Sieler F, Sievert SM, Steger J, Tinta T, Winter TRM, Bright Z, Coffield R, Hill C, Ingram K, and Paris A
- Subjects
- Animals, Oceans and Seas, Seawater microbiology, Geologic Sediments microbiology, Hydrothermal Vents microbiology, Larva, Polychaeta physiology, Ecosystem
- Abstract
It was once believed that only microbes and viruses inhabited the subseafloor crust beneath hydrothermal vents. Yet, on the seafloor, animals like the giant tubeworm Riftia pachyptila thrive. Their larvae are thought to disperse in the water column, despite never being observed there. We hypothesized that these larvae travel through the subseafloor via vent fluids. In our exploration, lifting lobate lava shelves revealed adult tubeworms and other vent animals in subseafloor cavities. The discovery of vent endemic animals below the visible seafloor shows that the seafloor and subseafloor faunal communities are connected. The presence of adult tubeworms suggests larval dispersal through the recharge zone of the hydrothermal circulation system. Given that many of these animals are host to dense bacterial communities that oxidize reduced chemicals and fix carbon, the extension of animal habitats into the subseafloor has implications for local and regional geochemical flux measurements. These findings underscore the need for protecting vents, as the extent of these habitats has yet to be fully ascertained., (© 2024. The Author(s).)
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- 2024
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32. Chemical Characterization and Assessment of Public Health Risk due to Inhalation of PM 2.5 in the City of Salamanca, Guanajuato.
- Author
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Castro Ramírez I, Rocha Amador DO, López Gutiérrez JM, Ramírez Mosqueda E, Cea Barcia GE, Ramos Patlán FD, and Costilla Salazar R
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- Humans, Mexico, Risk Assessment, Environmental Monitoring, Public Health, Adult, Child, Cities, Particulate Matter analysis, Air Pollutants analysis, Inhalation Exposure statistics & numerical data
- Abstract
In this study, we conducted an analysis of health risks faced by residents of Salamanca, Mexico, who were exposed to fine particulate matter with a diameter of 2.5 μm (PM
2.5 ) through inhalation. The characterization and analysis of these particulate matter samples were undertaken. A total of 131 samples were collected from two different sites: 65 from the Red Cross site (RC) and 66 from the Integral Family Development site (DIF) in 2014-2015. These samples were analyzed for a set of chemical components, including metals and ions. Non-cancerous health risk levels associated with PM2.5 exposure through the human respiratory system, as per the WHO benchmark (assigned a value of 1), revealed notable risk values for two elements: Manganese (Mn) with a range of 1.19-2.12 in the adult population and 1.59-2.84 in the child population, and Nickel (Ni) with a uniform risk value of 1.39 for both evaluated population groups. However, concerns arose regarding potential non-cancerous effects as the cumulative risk levels for various assessed elements showed elevated indices. These ranged from 3.81 to 4.4 in adults and 4.48-5.24 in children. This study provided comprehensive data on composition and its potential impact on human health, offering valuable insights for the implementation of mitigation measures aimed at reducing inhalation-related exposure., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2024
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33. Correction: Castelan-Ramírez et al. Schwann Cell Autophagy and Necrosis as Mechanisms of Cell Death by Acanthamoeba . Pathogens 2020, 9 , 458.
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Castelan-Ramírez I, Salazar-Villatoro L, Chávez-Munguía B, Salinas-Lara C, Sánchez-Garibay C, Flores-Maldonado C, Hernández-Martínez D, Anaya-Martínez V, Ávila-Costa MR, Méndez-Cruz AR, and Omaña-Molina M
- Abstract
In the original publication [...].
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- 2024
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34. Estimation of predation rate and handling time of boll weevil larvae by Marava arachidis (Dermaptera: Labiidae) using different mathematical methods.
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Silva Neto JG, Silva TGF, Salustino AS, Leite EL, Abreu KG, Silva AS, Batista JL, Brito CH, Araújo EK, Cândido BAP, Silva IVI, Viagem CRSM, Ramírez IMB, Correia Neto DF, and Malaquias JB
- Subjects
- Animals, Time Factors, Population Density, Neoptera physiology, Predatory Behavior physiology, Larva physiology, Weevils physiology, Pest Control, Biological
- Abstract
Anthonomus grandis grandis (Coleoptera: Curculionidae) is a pest with a large potential for destruction in cotton crops, causing damage to the cotton reproductive structures. The earwig Marava arachidis (Dermaptera: Labiidae), is an important reference as a predator in several crops and being easy to rear in the laboratory. To analyze the potential biocontrol of M. arachidis of A. grandis grandis larvae, a study of predatory capacity was conducted using a functional response model. A. grandis grandis larvae were exposed to the predator at densities 1, 2, 4, 6, and 8 larvae (= prey/predator / Petri dish), with 30 replications at each density. Contact between the predator and the prey occurred for 24 hours; after this period, the level of predation of M. arachidis was assessed based on the proportion of preyed larvae. The linear logistic regression coefficient was used with a beta-binomial generalized linear model to determine the functional response. The negative signal of the linear coefficient and the goodness-of-fit tests revealed a quadratic or type II functional response, with the number of prey varying from 1.00 larva (density of 1 larva/predator) to 6.50 larvae (density of 8 larvae/predator). Therefore, the results of the present study demonstrate a high predatory capacity of M. arachidis on A. grandis grandis larvae.
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- 2024
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- View/download PDF
35. Frequency of Leptospira in the Blood of Patients with Fever of Unknown Origin in Sinaloa, Mexico.
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Lagarde-Guerrero R, Navarro-Arias MJ, Duran-Pérez SA, Osuna-Ramírez I, Osuna-Martínez LU, Gonzáles-Durán E, and Rendon-Maldonado JG
- Abstract
Background : Leptospira is a genus of bacteria that causes the zoonotic disease known as leptospirosis, which mainly affects countries with tropical and subtropical climates. Its prevalence may be underestimated because the initial stage of the infection is characterized by presenting a febrile condition that is easily confused with other diseases, such as dengue. This work reports the frequency of leptospirosis in the blood of patients with febrile symptoms of unknown origin. Materials and Methods: A total of 218 peripheral blood samples were analyzed from volunteer participants from Culiacan Sinaloa in June 2019, one half corresponded to patients with undiagnosed febrile symptoms and the other half to asymptomatic volunteers. Data collected included the age and sex of the participants. Leptospira was detected by qPCR using a fragment of the lipL32 gene from the bacteria's genome as a target. Fisher's exact test was used as a statistical method to estimate the relationship between the infection and the data collected. Results: The study group comprised 134 female and 84 male patients ranging from ages 1 to 92 years, averaging 41 years. In this study, Leptospira infection was identified in the blood of 22/218 participating volunteers (10.09%), of which 20/109 (18.34%) presented febrile symptoms, whereas 2/109 (1.83%) were asymptomatic. The most affected participants were women with ages between 27 and 59 years. However, the analysis of the relationship between infection and the variables studied did not show statistical significance. Conclusions : Leptospirosis was detected in blood samples from patients with undiagnosed febrile illness and asymptomatic symptoms in Sinaloa. The lipL32 gene is useful as a target in identifying Leptospira in human blood in the acute phase of the disease.
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- 2024
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36. Statistical Interdependence between Daily Precipitation and Extreme Daily Temperature in Regions of Mexico and Colombia.
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Zabaleta-Ortega Á, Mercado-Fernández T, Reyes-Ramírez I, Angulo-Brown F, and Guzmán-Vargas L
- Abstract
We study the statistical interdependence between daily precipitation and daily extreme temperature for regions of Mexico (14 climatic stations, period 1960-2020) and Colombia (7 climatic stations, period 1973-2020) using linear (cross-correlation and coherence) and nonlinear (global phase synchronization index, mutual information, and cross-sample entropy) synchronization metrics. The information shared between these variables is relevant and exhibits changes when comparing regions with different climatic conditions. We show that precipitation and temperature records from La Mojana are characterized by high persistence, while data from Mexico City exhibit lower persistence (less memory). We find that the information exchange and the level of coupling between the precipitation and temperature are higher for the case of the La Mojana region (Colombia) compared to Mexico City (Mexico), revealing that regions where seasonal changes are almost null and with low temperature gradients (less local variability) tend to display higher synchrony compared to regions where seasonal changes are very pronounced. The interdependence characterization between precipitation and temperature represents a robust option to characterize and analyze the collective dynamics of the system, applicable in climate change studies, as well as in changes not easily identifiable in future scenarios.
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- 2024
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37. Imidazole Carbamates as a Promising Alternative for Treating Trichomoniasis: In Vitro Effects on the Growth and Gene Expression of Trichomonas vaginalis .
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Martínez-Rosas V, Navarrete-Vázquez G, Ortega-Cuellar D, Arreguin-Espinosa R, Pérez de la Cruz V, Calderón-Jaimes E, Enríquez-Flores S, Wong-Baeza C, Baeza-Ramírez I, Morales-Luna L, Vázquez-Bautista M, Rojas-Alarcón MA, Hernández-Ochoa B, and Gómez-Manzo S
- Subjects
- Humans, Metronidazole pharmacology, Metronidazole chemistry, Gene Expression Regulation drug effects, Trophozoites drug effects, Trichomonas vaginalis drug effects, Trichomonas vaginalis genetics, Trichomonas vaginalis growth & development, Imidazoles pharmacology, Imidazoles chemistry, Carbamates pharmacology, Carbamates chemistry
- Abstract
Metronidazole (MTZ) is the most common drug used against Trichomonas vaginalis ( T. vaginalis ) infections; however, treatment failures and high rates of recurrence of trichomoniasis have been reported, suggesting the presence of resistance in T. vaginalis to MTZ. Therefore, research into new therapeutic options against T. vaginalis infections has become increasingly urgent. This study investigated the trichomonacidal activity of a series of five imidazole carbamate compounds (AGR-1, AGR-2, AGR-3, AGR-4, and AGR-5) through in vitro susceptibility assays to determine the IC
50 value of each compound. All five compounds demonstrated potent trichomonacidal activity, with IC50 values in the nanomolar range and AGR-2 being the most potent (IC50 400 nM). To gain insight into molecular events related to AGR-induced cell death in T. vaginalis , we analyzed the expression profiles of some metabolic genes in the trophozoites exposed to AGR compounds and MTZ. It was found that both AGR and MTZ compounds reduced the expression of the glycolytic genes ( CK , PFK , TPI , and ENOL ) and genes involved in metabolism ( G6PD , TKT , TALDO , NADHOX , ACT , and TUB ), suggesting that disturbing these key metabolic genes alters the survival of the T. vaginalis parasite and that they probably share a similar mechanism of action. Additionally, the compounds showed low cytotoxicity in the Caco-2 and HT29 cell lines, and the results of the ADMET analysis indicated that these compounds have pharmacokinetic properties similar to those of MTZ. The findings offer significant insights that can serve as a basis for future in vivo studies of the compounds as a potential new treatment against T. vaginalis .- Published
- 2024
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38. Effect of a multidisciplinary team in the management of vascular access for hemodialysis.
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Leblic Ramírez I, Riera Del Moral L, Sánchez Villanueva R, Stefanov Kiuri S, Álvarez García L, Echarri Carrillo R, Gallegos Villalobos Á, and Fernandez Heredero Á
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- Humans, Male, Female, Vascular Access Devices, Middle Aged, Arteriovenous Shunt, Surgical, Aged, Renal Dialysis methods, Patient Care Team
- Published
- 2024
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- View/download PDF
39. NME1 and DCC variants are associated with susceptibility and tumor characteristics in Mexican patients with colorectal cancer.
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Márquez-González RM, Saucedo-Sariñana AM, de Jesús Tovar-Jacome C, Barros-Núñez P, Gallegos-Arreola MP, Orozco-Gutiérrez MH, Mariscal-Ramírez I, Pineda-Razo TD, Alcaraz-Wong AA, Marín-Contreras ME, and Rosales-Reynoso MA
- Subjects
- Humans, Middle Aged, Genetic Predisposition to Disease, Polymorphism, Single Nucleotide, Genotype, Case-Control Studies, DCC Receptor genetics, NM23 Nucleoside Diphosphate Kinases genetics, Stomach Neoplasms genetics, Colorectal Neoplasms epidemiology, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology
- Abstract
Background: Colorectal cancer (CRC) ranks third in cancer incidence globally and is the second leading cause of cancer-related mortality. The nucleoside diphosphate kinase 1 (NME1) and netrin 1 receptor (DCC) genes have been associated with resistance against tumorigenesis and tumor metastasis. This study investigates the potential association between NME1 (rs34214448 G > T and rs2302254 C > T) and DCC (rs2229080 G > C and rs714 A > G) variants and susceptibility to colorectal cancer development., Methods: Samples from 232 colorectal cancer patients and 232 healthy blood donors underwent analysis. Variants were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology. Associations were assessed using odds ratios (OR), and the p values were adjusted with Bonferroni test., Results: Individuals carrying the G/T and T/T genotypes for the NME1 rs34214448 variant exhibited a higher susceptibility for develop colorectal cancer (OR = 2.68, 95% CI: 1.76-4.09, P = 0.001 and OR = 2.47, 95% CI: 1.37-4.47, P = 0.001, respectively). These genotypes showed significant associations in patients over 50 years (OR = 2.87, 95% CI: 1.81-4.54, P = 0.001 and OR = 2.99, 95% CI: 1.54-5.79, P = 0.001 respectively) and with early Tumor-Nodule-Metastasis (TNM) stage (P = 0.001), and tumor location in the rectum (P = 0.001). Furthermore, the DCC rs2229080 variant revealed that carriers of the G/C genotype had an increased risk for develop colorectal cancer (OR = 2.00, 95% CI: 1.28-3.11, P = 0.002) and were associated with age over 50 years, sex, and advanced TNM stages (P = 0.001)., Conclusions: These findings suggest that the NME1 rs34214448 and DCC rs2229080 variants play a significant role in colorectal cancer development., (© 2024. The Author(s).)
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- 2024
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40. High-Throughput Spectroscopic Analysis of mRNA Capping Level.
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Redwood-Sawyerr C, Aw R, Di Blasi R, Moya-Ramírez I, Kontoravdi C, Ceroni F, and Polizzi K
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- Animals, Spectrum Analysis, RNA, Messenger genetics, Transfection, Polyadenylation, Mammals
- Abstract
Eukaryotic mRNAs are characterized by terminal 5' cap structures and 3' polyadenylation sites, which are essential for posttranscriptional processing, translation initiation, and stability. Here, we describe a novel biosensor method designed to detect the presence of both cap structures and polyadenylation sites on mRNA molecules. This novel biosensor is sensitive to mRNA degradation and can quantitatively determine capping levels of mRNA molecules within a mixture of capped and uncapped mRNA molecules. The biosensor displays a constant dynamic range between 254 nt and 6507 nt with reproducible sensitivity to increases in capping level of at least 20% and a limit of detection of 2.4 pmol of mRNA. Overall, the biosensor can provide key information about mRNA quality before mammalian cell transfection., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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- View/download PDF
41. Label-free SERS assay combined with multivariate spectral data analysis for lamotrigine quantification in human serum.
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Badillo-Ramírez I, Janssen SAJ, Soufi G, Slipets R, Zór K, and Boisen A
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- Humans, Lamotrigine, Data Analysis, Anticonvulsants, Spectrum Analysis, Raman methods
- Abstract
Considering the need for a more time and cost-effective method for lamotrigine (LTG) detection in clinics we developed a fast and robust label-free assay based on surface-enhanced Raman scattering (SERS) for LTG quantification from human serum. The optimization and application of the developed assay is presented showing the: (i) exploration of different methods for LTG separation from human serum; (ii) implementation of a molecular adsorption step on an ordered Au nanopillar SERS substrate; (iii) adaptation of a fast scanning of the SERS substrate, performed with a custom-built compact Raman spectrometer; and (iv) development of LTG quantification methods with univariate and multivariate spectral data analysis. Our results showed, for the first time, the SERS-based characterization of LTG and its label-free identification in human serum. We found that combining a miniaturized solid phase extraction, as sample pre-treatment with the SERS assay, and using a multivariate model is an optimal strategy for LTG quantification in human serum in a linear range from 9.5 to 75 μM, with LoD and LoQ of 3.2 μM and 9.5 μM, respectively, covering the suggested clinical therapeutic window. We also showed that the developed assay allowed for quantifying LTG from human serum in the presence of other drugs, thereby demonstrating the robustness of label-free SERS. The sensing approach and instrumentation can be further automated and integrated in devices that can advance the drug monitoring in real clinical settings., (© 2023. The Author(s).)
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- 2023
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42. Extracellular Vesicles Secreted by Acanthamoeba culbertsoni Have COX and Proteolytic Activity and Induce Hemolysis.
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Sierra-López F, Castelan-Ramírez I, Hernández-Martínez D, Salazar-Villatoro L, Segura-Cobos D, Flores-Maldonado C, Hernández-Ramírez VI, Villamar-Duque TE, Méndez-Cruz AR, Talamás-Rohana P, and Omaña-Molina M
- Abstract
Several species of Acanthamoeba genus are potential pathogens and etiological agents of several diseases. The pathogenic mechanisms carried out by these amoebae in different target tissues have been documented, evidencing the relevant role of contact-dependent mechanisms. With the purpose of describing the pathogenic processes carried out by these protozoans more precisely, we considered it important to determine the emission of extracellular vesicles (EVs) as part of the contact-independent pathogenicity mechanisms of A. culbertsoni , a highly pathogenic strain. Through transmission electronic microscopy (TEM) and nanoparticle tracking analysis (NTA), EVs were characterized. EVs showed lipid membrane and a size between 60 and 855 nm. The secretion of large vesicles was corroborated by confocal and TEM microscopy. The SDS-PAGE of EVs showed proteins of 45 to 200 kDa. Antigenic recognition was determined by Western Blot, and the internalization of EVs by trophozoites was observed through Dil-labeled EVs. In addition, some EVs biological characteristics were determined, such as proteolytic, hemolytic and COX activity. Furthermore, we highlighted the presence of leishmanolysin in trophozites and EVs. These results suggest that EVs are part of a contact-independent mechanism, which, together with contact-dependent ones, allow for a better understanding of the pathogenicity carried out by Acanthamoeba culbertsoni .
- Published
- 2023
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43. Measurement of Cutting Temperature in Interrupted Machining Using Optical Spectrometry.
- Author
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Espinoza-Torres I, Martínez-Ramírez I, Sierra-Hernández JM, Jauregui-Vazquez D, Gutiérrez-Rivera ME, Carmen FJT, and Lozano-Hernández T
- Abstract
This research presents an experimental study focused on measuring temperature at the tool flank during the up-milling process at high cutting speed. The proposed system deals with emissivity compensation through a two-photodetector system and during calibration. A ratio pyrometer composed of two photodetectors and a multimode fiber-optic coupler is employed to capture the radiation emitted by the cutting insert. The pyrometer is calibrated using an innovative calibration system that addresses theoretical discrepancies arising from various factors affecting the measurement of cutting temperature. This calibration system replicates the milling process to generate a calibration curve. Experimentally, AISI 4140 steel is machined with coated tungsten carbide inserts, using cutting speeds of 300 and 400 m/min, and feed rates of 0.08 and 0.16 mm/tooth. The results reveal a maximum recorded cutting temperature of 518 °C and a minimum of 304 °C. The cutting temperature tends to increase with higher cutting speeds and feed rates, with cutting speed being the more influential factor in this increase. Both the pyrometer calibration and experimental outcomes yield satisfactory results. Finally, the results showed that the process and the device prove to be a convenient, effective, and precise method of measuring cutting temperature in machine processes.
- Published
- 2023
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- View/download PDF
44. SYNAPSES. A European observational study to evaluate the safety and the effectiveness of safinamide in routine clinical practice: post-hoc analysis of the Spanish study population.
- Author
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Kulisevsky J, Esquivel A, Freire-Álvarez E, Gómez-Esteban JC, Legarda-Ramírez I, Avilés A, and Mata-Álvarez-Santullano M
- Subjects
- Humans, Synapses, Benzylamines adverse effects, Levodopa adverse effects, Parkinson Disease drug therapy
- Abstract
Introduction: Parkinson's Disease (PD) is a progressive age-related neurodegenerative condition requiring new therapeutic alternatives. Safinamide, a novel levodopa add-on therapy, positively affects disease fluctuations by modulating both dopaminergic and glutamatergic systems. To further investigate the use of safinamide in European routine clinical practice, the present post-hoc analysis aimed to understand safinamide's safety profile within the Spanish study population., Patients and Methods: Five hundred eleven Spanish patients with PD were evaluated at baseline, four (±1), eight (±1), and 12 (±1) months after initiating safinamide treatment. Unified Parkinson's Disease Rating Scale (UPDRS) total score and UPDRS part III score during on time were used to measure the overall severity of PD and motor complications, respectively, while the severity of adverse events was evaluated following the investigators' criteria., Results: Safinamide showed a favourable safety profile within the Spanish study population, although prescription to patients with psychiatric conditions and off-label use were more frequent than in the European study population. In Spain, clinically meaningful improvements were observed in UPDRS scores when safinamide was used as the only add-on therapy to levodopa (57.4% and 53.7% of patients) and when switching from rasagiline (55.1% of patients). Motor complications were reduced from 83.2% to 63.3% after the study period. Increased safety concerns were undetected in any patient subgroup, although patients with cognitive impairment showed a slightly higher frequency of adverse events., Conclusions: This subanalysis further supports safinamide use as a safe and efficacious option for the management of motor fluctuations in different subgroups of levodopa-treated patients. However, safinamide should be used with caution in patients with cognitive impairment.
- Published
- 2023
- Full Text
- View/download PDF
45. [Clinical experience on the management of post-traumatic bone defects of long bones treated with the membrane induction technique].
- Author
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Quiroz-Williams J, Gaytán-Fernández S, Barragán-Hervella RG, Morgan-Padrón S, Rojas-Durán O, Martínez-Asención JP, and Castillo-Ramírez IO
- Subjects
- Humans, Adult, Middle Aged, Treatment Outcome, Bone Transplantation methods, Retrospective Studies, Quality of Life, Fractures, Bone
- Abstract
Introduction: bone defects drastically alter the patient's quality of life, and can produce serious sequelae such as dysfunctional shortening, angular deformity, joint stiffness and irreversible gait disorder., Objective: to describe the experience of managing post-traumatic bone defects of long bones treated with the membrane induction technique., Material and Methods: retrospective observational study of patients with bone defects greater than 3 cm in diaphyseal pseudoarthrosis of long bones, who underwent the Masquelet technique. Period taken from January 2019 to January 2021. All patients underwent vigorous debridement and stabilization of the fracture, to then place the cement spacer. Spacer removal was at 4-6 weeks. The degree of consolidation was assessed, as well as the evolution of the treatment., Result: 25 patients were included in the study; the mean age was 36.8 ± 8.9 years. Diameter of bone losses was 3 to 10 cm (84%) and > 10 cm (16%). Bone consolidation occurred in patients with a defect < 10 cm (16%). 32% of patients presented some complication., Conclusions: bone union was achieved only in a few patients with defects smaller than 10 cm, requiring alternative procedures in most cases. Proper selection of patients is required.
- Published
- 2023
46. Hypomagnesemia and 25-hydroxyvitamin D deficiency in patients with long COVID.
- Author
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Guerrero-Romero F, Gamboa-Gómez CI, Rodríguez-Morán M, Orrante M, Rosales-Galindo E, Cisneros-Ramírez I, Arce-Quiñones M, Orona-Díaz K, Simental-Mendia LE, and Martínez-Aguilar G
- Subjects
- Adult, Humans, Post-Acute COVID-19 Syndrome, Cross-Sectional Studies, Calcifediol, Magnesium, COVID-19 complications, Vitamin D analogs & derivatives
- Abstract
Clinical manifestations related to hypomagnesemia and/or deficiency of vitamin D are frequent in patients with an extended course of coronavirus disease-2019 (long COVID). To evaluate hypomagnesemia and hydroxyvitamin D deficiency in patients with long COVID. A total of 125 adults with a diagnosis of long COVID were enrolled in a cross-sectional study. Participants were allocated into a risk (hypomagnesemia and hydroxyvitamin D deficiency) or control (serum magnesium and hydroxyvitamin D within normal ranges) group. Hypomagnesemia and 25-hydroxyvitamin D deficiency were defined based on serum level ≤1.8 mg/dL and <30 ng/mL, respectively. The number of clinical manifestations of long COVID were significantly higher in the risk compared to the control group. Fatigue, memory loss, attention disorders, joint pain, anxiety, sleep disorders, myalgia, and depression, all of which are related to hypomagnesemia and/or 25-hydroxyvitamin D deficiency, were among the 10 most frequent manifestations in the risk group. The adjusted odds ratio for the association between hypomagnesemia and hydroxyvitamin D deficiency during long COVID was 3.1; 95% CI 2.3-12.4, p=0.005. Our results show that patients suffering with long COVID had a deficiency in magnesium and 25-hydroxyvitamin D which correlated with the number of associated clinical manifestations.
- Published
- 2023
- Full Text
- View/download PDF
47. Unraveling the Role of EV-Derived miR-150-5p in Prostate Cancer Metastasis and Its Association with High-Grade Gleason Scores: Implications for Diagnosis.
- Author
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Cruz-Burgos M, Cortés-Ramírez SA, Losada-García A, Morales-Pacheco M, Martínez-Martínez E, Morales-Montor JG, Servín-Haddad A, Izquierdo-Luna JS, Rodríguez-Martínez G, Ramos-Godínez MDP, González-Covarrubias V, Cañavera-Constantino A, González-Ramírez I, Su B, Leong HS, and Rodríguez-Dorantes M
- Abstract
Metastasis remains the leading cause of mortality in prostate cancer patients. The presence of tumor cells in lymph nodes is an established prognostic indicator for several cancer types, such as melanoma, breast, oral, pancreatic, and cervical cancers. Emerging evidence highlights the role of microRNAs enclosed within extracellular vesicles as facilitators of molecular communication between tumors and metastatic sites in the lymph nodes. This study aims to investigate the potential diagnostic utility of EV-derived microRNAs in liquid biopsies for prostate cancer. By employing microarrays on paraffin-embedded samples, we characterized the microRNA expression profiles in metastatic lymph nodes, non-metastatic lymph nodes, and primary tumor tissues of prostate cancer. Differential expression of microRNAs was observed in metastatic lymph nodes compared to prostate tumors and non-metastatic lymph node tissues. Three microRNAs (miR-140-3p, miR-150-5p, and miR-23b-3p) were identified as differentially expressed between tissue and plasma samples. Furthermore, we evaluated the expression of these microRNAs in exosomes derived from prostate cancer cells and plasma samples. Intriguingly, high Gleason score samples exhibited the lowest expression of miR-150-5p compared to control samples. Pathway analysis suggested a potential regulatory role for miR-150-5p in the Wnt pathway and bone metastasis. Our findings suggest EV-derived miR-150-5p as a promising diagnostic marker for identifying patients with high-grade Gleason scores and detecting metastasis at an early stage.
- Published
- 2023
- Full Text
- View/download PDF
48. Taurine, a Component of the Tear Film, Exacerbates the Pathogenic Mechanisms of Acanthamoeba castellanii in the Ex Vivo Amoebic Keratitis Model.
- Author
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Salazar-Villatoro L, Chávez-Munguía B, Guevara-Estrada CE, Lagunes-Guillén A, Hernández-Martínez D, Castelan-Ramírez I, and Omaña-Molina M
- Abstract
Acanthamoeba spp. is the etiological agent of amoebic keratitis. In this study, the effect of taurine in physiological concentrations in tears (195 μM) on trophozoites of Acanthamoeba castellanii through the ex vivo amoebic keratitis model was evaluated. Trophozoites were coincubated with the Syrian golden hamster cornea ( Mesocricetus auratus ) for 3 and 6 h. Group 1: Control (-). Corneas coincubated with amoebic culture medium and taurine. Group 2: Control (+). Corneas coincubated with trophozoites without taurine. Group 3: Corneas coincubated with taurine 15 min before adding trophozoites. Group 4: Trophozoites coincubated 15 min with taurine before placing them on the cornea. Group 5: Corneas coincubated for 15 min with trophozoites; subsequently, taurine was added. Results are similar for both times, as evaluated by scanning electron microscopy. As expected, in the corneas of Group 1, no alterations were observed in the corneal epithelium. In the corneas of Group 2, few adhered trophozoites were observed on the corneal surface initiating migrations through cell junctions as previously described; however, in corneas of Groups 3, 4 and 5, abundant trophozoites were observed, penetrating through different corneal cell areas, emitting food cups and destabilizing corneal surface in areas far from cell junctions. Significant differences were confirmed in trophozoites adherence coincubated with taurine ( p < 0.05). Taurine does not prevent the adhesion and invasion of the amoebae, nor does it favor its detachment once these have adhered to the cornea, suggesting that taurine in the physiological concentrations found in tears stimulates pathogenic mechanisms of A. castellanii .
- Published
- 2023
- Full Text
- View/download PDF
49. Associations between Menopausal Hormone Therapy and Colorectal, Lung, or Melanoma Cancer Recurrence and Mortality: A Narrative Review.
- Author
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Fiol G, Lete I, Nieto L, Santaballa A, Pla MJ, Baquedano L, Calaf J, Coronado P, de la Viuda E, Llaneza P, Otero B, Sánchez-Méndez S, Ramírez I, and Mendoza N
- Abstract
Objective: to develop eligibility criteria for use in non-gynecological cancer patients. Methods: We searched all the articles published in peer-reviewed journals up to March 2021. We utilized the PICOS standards and the following selection criteria: menopausal women with a history of non-gynecological and non-breast cancer who underwent hormone replacement therapy (HRT) using various preparations (oestrogens alone or in combination with a progestogen, tibolone, or tissue selective oestrogen complex) and different routes of administration (including oral, transdermal, vaginal, or intra-nasal). We focused on randomized controlled trials as well as relevant extension studies or follow-up reports, specifically examining recurrence and mortality outcomes. Results: Women colorectal cancer survivors who use MHT have a lower risk of death from any cause than those survivors who do not use MHT. Women who are skin melanoma survivors using MHT have a longer survival rate than non-MHT survivors. There is no evidence that women lung cancer survivors who use MHT have a different survival rate than those who do not use MHT. Conclusions: MHT is safe for women who have a history of colorectal, lung, or skin melanoma cancers.
- Published
- 2023
- Full Text
- View/download PDF
50. Spent coffee grounds as feedstock for the production of biosurfactants and the improved recovery of melanoidins.
- Author
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Moya-Ramírez I, Pegalajar-Robles ME, Debiasi Alberton M, Rufián-Henares JA, Fernández-Arteaga A, Garcia-Roman M, and Altmajer-Vaz D
- Subjects
- Polymers, Biotechnology, Coffee, Antioxidants
- Abstract
Spent coffee grounds (SCG) are wastes generated in high amounts worldwide. Their composition makes them a promising feedstock for biotechnological processes. Here we show that the production of the biosurfactant surfactin by submerged culture of a Bacillus subtilis strain growing on SCG is possible, reaching concentrations up to 8.8 mg/L when using SCG at 8.3 g/L in the medium. In addition, we report a synergy between the production of surfactin and the recovery of melanoidins, an added-value compound already present in SCG. More specifically, the concentration of melanoidins in the culture medium increased between 2.1 and 2.5 times thanks to the presence of the B. subtilis in the culture. Furthermore, we have observed a strong interaction between surfactin and melanoidin aggregates through dynamic light scattering measurements, and that both of them can be co-purified with an acid precipitation. We have also characterized the interfacial and antioxidant properties of the cell-free supernatant and surfactin extract, as well as the distribution of the congeners of the biosurfactant. Altogether, this work describes a promising approach to obtain biosurfactants and antioxidant molecules in a single operation, which can be used to design several new formulations of interest for bioremediation, amendment of soils, food and cosmetics., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
- View/download PDF
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