1. The Cl - -channel TMEM16A is involved in the generation of cochlear Ca 2+ waves and promotes the refinement of auditory brainstem networks in mice.
- Author
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Maul A, Huebner AK, Strenzke N, Moser T, Rübsamen R, Jovanovic S, and Hübner CA
- Subjects
- Action Potentials, Animals, Auditory Pathways, Brain Waves, Chloride Channels metabolism, Hair Cells, Auditory metabolism, Hair Cells, Auditory, Inner metabolism, Mice, Mice, Knockout, Anoctamin-1 metabolism, Brain Stem metabolism, Calcium metabolism, Cochlea metabolism, Hearing, Neurons metabolism
- Abstract
Before hearing onset (postnatal day 12 in mice), inner hair cells (IHCs) spontaneously fire action potentials, thereby driving pre-sensory activity in the ascending auditory pathway. The rate of IHC action potential bursts is modulated by inner supporting cells (ISCs) of Kölliker's organ through the activity of the Ca
2+ -activated Cl- -channel TMEM16A (ANO1). Here, we show that conditional deletion of Ano1 ( Tmem16a ) in mice disrupts Ca2+ waves within Kölliker's organ, reduces the burst-firing activity and the frequency selectivity of auditory brainstem neurons in the medial nucleus of the trapezoid body (MNTB), and also impairs the functional refinement of MNTB projections to the lateral superior olive. These results reveal the importance of the activity of Kölliker's organ for the refinement of central auditory connectivity. In addition, our study suggests the involvement of TMEM16A in the propagation of Ca2+ waves, which may also apply to other tissues expressing TMEM16A., Competing Interests: AM, AH, NS, TM, RR, SJ, CH No competing interests declared, (© 2022, Maul et al.)- Published
- 2022
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