739 results on '"Qin, D"'
Search Results
2. The Prognostic Significance of the CALLY Index in Ampullary Carcinoma: An Inflammation-Nutrition Retrospective Analysis
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Xi P, Huang G, Huang K, Qin D, Yao Z, Jiang L, Zhu Q, and He C
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ampullary carcinoma ,prognosis ,inflammation-nutritional-index ,competing risk analysis ,propensity matching analysis ,Pathology ,RB1-214 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Pu Xi,1,* Guizhong Huang,1,* Kewei Huang,2,* Dailei Qin,1 Zehui Yao,1 Lingmin Jiang,1 Qi Zhu,1 Chaobin He1 1Department of Pancreatobiliary Surgery, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, People’s Republic of China; 2State Key Laboratory of Oncology in South China, Department of Clinical Laboratory, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chaobin He, Department of Pancreatobiliary Surgery, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, 651 Dongfeng Road E, Guangzhou, Guangdong, 510060, People’s Republic of China, Email hechb@sysucc.org.cnBackground: As a novel inflammatory-nutritional biomarker, the C-reactive protein–albumin–lymphocyte (CALLY) index has demonstrated significant prognostic value in various malignancies. However, research on its association with the prognosis of ampullary carcinoma (AC) is rare. This study aims to investigate the relationship between the CALLY index and the prognosis of patients with AC.Methods: We retrospectively analyzed data from 201 patients with AC at Sun Yat-sen University Cancer Center. Several clinicopathological factors and biomarkers were included in the study. Univariate and multivariate Cox regression analyses, along with competing risk analysis, were performed to identify prognostic factors for AC after pancreaticoduodenectomy (PD). Only factors with significant results in univariate analysis were included in multivariate analysis. To ensure the robustness of our findings, propensity score matching (PSM) analyses were conducted to assess survival differences according to the CALLY index.Results: The univariate and multivariate Cox regression analyses revealed that pathological type, N stage, T stage, postoperative chemotherapy regimen, and the CALLY index were all statistically significant prognostic factors for patients with AC after PD (all P values < 0.05). Taking into account non-cancer-related mortality as competing hazards, these factors remained significant predictors (all P values < 0.05). After PSM, the survival advantage observed between the low and high CALLY groups remained discernible and consistent.Conclusion: This study indicated that a reduced CALLY index correlates with a poorer cancer-specific survival in AC patients after PD, highlighting its utility as a prognostic marker for this condition.Keywords: ampullary carcinoma, prognosis, inflammation-nutritional-index, competing risk analysis, propensity matching analysis
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- 2025
3. Retrospective Analysis of Sacubitril/Valsartan vs Benazepril for Treating Heart Failure Following Acute Myocardial Infarction
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Zhao L, Ren Y, Qin D, Yang X, Chen Z, and Zhang N
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sacubitril/valsartan ,heart failure following acute myocardial infarction ,efficacy ,cardiac function ,Medicine (General) ,R5-920 - Abstract
Li Zhao,1 Yuanyuan Ren,1 Donghui Qin,2 Xue Yang,1 Zhuo Chen,1 Na Zhang1 1Department of Cardiology, The Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, 161000, People’s Republic of China; 2General Hospital, Qiqihar City Second Hospital, Qiqihar, People’s Republic of ChinaCorrespondence: Na Zhang, Email 13904523506@163.comObjective: To retrospectively compare the efficacy of Sacubitril/Valsartan and Benazepril in the treatment of heart failure in patients following acute myocardial infarction.Methods: A retrospective analysis of clinical data was conducted for 103 patients with heart failure following acute myocardial infarction admitted to our hospital from January 2021 to January 2024. All patients met complete inclusion and exclusion criteria. Based on the treatment interventions received, they were divided into a control group (n=51) and an observation group (n=52). All patients received percutaneous coronary intervention (PCI) and conventional drug treatment upon admission. The control group received additional treatment with benazepril, while the observation group received Sacubitril/Valsartan on top of the baseline treatment. A comparison was made between the two groups in terms of clinical treatment outcomes, cardiac function indicators [left ventricular end-systolic volume (LVESV), left ventricular end-diastolic volume (LVEDD), left ventricular ejection fraction (LVEF)], levels of inflammatory markers [high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6)], N-terminal pro-B-type natriuretic peptide (NT-proBNP), incidence of adverse reactions, major adverse cardiac events (MACEs), and 6-minute walking distance (6MWD).Results: No patients were lost to follow-up. After six months of treatment, the observation group demonstrated significantly greater improvements in left ventricular function parameters (LVESV, LVEDD, and LVEF) and reductions in inflammatory markers (hs-CRP, IL-6) and NT-proBNP levels compared to the control group (P < 0.05). The observation group also had a significantly lower incidence of major adverse cardiac events (MACEs) (11.54% vs 31.37%, P < 0.05) and a greater improvement in 6-minute walking distance (P < 0.05). The incidence of adverse reactions was comparable between the two groups (P > 0.05).Conclusion: Sacubitril/Valsartan is a safe and effective treatment for heart failure post-AMI, offering significant improvements in cardiac function, inflammatory response, exercise capacity, and a reduction in MACE risk.Keywords: sacubitril/valsartan, heart failure following acute myocardial infarction, efficacy, cardiac function
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- 2024
4. Exploring the Bidirectional Effects of Gut Microbiota and Short-Chain Fatty Acids on Urticaria Subtypes Through Mendelian Randomization and Mediation Analysis
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Xiao X, Xue P, Yan X, Li Y, Shi Y, Qin H, Qin D, Cao W, Zou Z, Wang L, Jin R, Yao J, and Li J
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urticaria ,short-chain fatty acids ,gut microbiota ,gut-skin axis ,mendelian randomization ,Dermatology ,RL1-803 - Abstract
Xianjun Xiao,1,* Peiwen Xue,1,* Xiangyun Yan,2,* Yanqiu Li,2,* Yunzhou Shi,2 Haiyan Qin,2 Di Qin,2 Wei Cao,2 Zihao Zou,2 Lu Wang,2 Rongjiang Jin,1 Ying Li,2 Junpeng Yao,2 Juan Li1,3 1School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, People’s Republic of China; 2Acupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, People’s Republic of China; 3Affiliated Sichuan Provincial Rehabilitation Hospital of Chengdu University of TCM, Chengdu, Sichuan, 611135, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junpeng Yao; Juan Li, Email yjp@cdutcm.edu.cn; 785939016@qq.comBackground: Emerging evidence links gut microbiota and their by-products, notably short-chain fatty acids (SCFAs), to urticaria. This study employs multiple Mendelian Randomization (MR) analyses to unravel the complex interactions among gut microbiota, SCFAs, and different subtypes of urticaria, aiming to elucidate the underlying mechanisms and enhance future clinical research.Methods: We analyzed published genome-wide association study (GWAS) summary statistics to identify associations between gut microbiota and three common subtypes of urticaria: spontaneous, dermatographic, and temperature-triggered. Initial two-sample and reverse MR analyses explored the causality in these relationships. Subsequent multivariate MR analyses investigated the role of SCFAs in modulating these interactions, with multiple sensitivity analyses to ensure robustness.Findings: Specific taxa were differently associated with various urticaria subtypes. From microbiota to urticaria: one taxon was negatively associated with dermatographic urticaria; seven taxa were negatively associated and four positively associated with temperature-triggered urticaria; four taxa were negatively associated and six positively associated with spontaneous urticaria. Conversely, from urticaria to microbiota: five taxa were negatively associated with dermatographic urticaria; four were negatively and two positively associated with temperature-triggered urticaria; and two were negatively associated with spontaneous urticaria. These associations were observed at a nominal significance level (P < 0.05). After applying Bonferroni correction for multiple testing, these associations did not reach statistical significance. The observed trends, however, provide insights into potential microbiota-urticaria interactions. Multivariate MR analyses elucidated the role of SCFAs, particularly acetate, which plays a crucial role in modulating immune response. Adjusting for acetate revealed direct effects of Actinobacteria, Bifidobacteriales, and Bifidobacteriaceae on spontaneous urticaria, with corresponding mediation effects of − 22%, − 24.9%, and − 24.9% respectively. Similarly, adjustments for Alcaligenaceae and Betaproteobacteria indicated significant negative effects of acetate on dermatographic and spontaneous urticaria, with mediation effects of − 21.7% and − 23.7%, respectively.Conclusion: This study confirms the interconnected roles of gut microbiota, SCFAs, and urticaria. It highlights SCFAs’ potential mediating role in influencing urticaria through microbiota, providing insights for future therapeutic strategies.Keywords: urticaria, short-chain fatty acids, gut microbiota, gut-skin axis, Mendelian randomization
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- 2024
5. Knowledge, Attitude, and Practice of the Gastroenterology Department Patients Towards Chronic Gastritis in Shanxi Region: A Cross-Sectional Study
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Qin D, Wang L, Ni Y, Shan Z, and Yang L
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knowledge ,attitude ,practice ,helicobacter pylori ,chronic gastritis ,cross-sectional study ,Medicine (General) ,R5-920 - Abstract
Dongyuan Qin,1 Lin Wang,2 Yahui Ni,1 Zhangtao Shan,1 Lifang Yang1 1Department of Gastroenterology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, People’s Republic of China; 2Department of Pathology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, People’s Republic of ChinaCorrespondence: Lin Wang, Department of Pathology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University, Taiyuan, 030032, People’s Republic of China, Email 13613411069@139.comBackground: Chronic gastritis affects more than half of the global population to varying extents.Objectives: This study aimed to investigate the knowledge, attitude, and practice (KAP) of patients admitted to the gastroenterology department in the Shanxi region concerning chronic gastritis.Methods: This study was conducted in Shanxi between April and July 2023. The participants were enrolled when they consulted at the clinic. Demographic characteristics and KAP scores were collected through a self-administered questionnaire. KAP scores > 60% were considered good. Structural equation modeling (SEM) was utilized to examine the relationships among the dimensions of knowledge, attitude, and practice.Results: A total of 416 valid questionnaires were collected. The median knowledge score was 28 (0– 60) (with possible values of 0– 60), the median attitude score was 60 (28– 77) (with possible values of 16– 80), and the median practice score was 45 (12– 60) (with possible values of 12– 60). Hence, 133, 379, and 343 participants had knowledge, attitude, and practice scores, respectively, above the 60% threshold. Significant positive correlations were found between knowledge and attitude (r=0.300, P< 0.001), knowledge and practice (r = 0.297, P< 0.001), and attitude and practice (r=0.353, P=0.004) through correlation analysis. Structural Equation Modeling (SEM) revealed that knowledge directly and significantly influenced attitude (β=0.643, P< 0.001), as well as practice (β=0.095, P=0.034), and attitude had a direct effect on practice (β=0.094, P=0.009).Conclusion: Insufficient knowledge, positive attitudes, and proactive practices concerning chronic gastritis were observed in patients in the gastroenterology department. Prioritizing patient education and addressing patient attitudes during clinical consultations can enhance healthcare practices and improve the management of chronic gastritis.Keywords: knowledge, attitude, practice, Helicobacter pylori, chronic gastritis, cross-sectional study
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- 2024
6. Controlled SPION-Exosomes Loaded with Quercetin Preserves Pancreatic Beta Cell Survival and Function in Type 2 Diabetes Mellitus
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Zhuang M, Rao L, Chen Y, Xiao S, Xia H, Yang J, Lv X, Qin D, and Zhu C
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quercetin ,exosome ,spion ,solubility ,targeted delivery ,islet function ,Medicine (General) ,R5-920 - Abstract
Manjiao Zhuang,1,* Lei Rao,2,3,* Yadi Chen,1 Shangying Xiao,1 Haishan Xia,1 Jiangyong Yang,2 Xiaohua Lv,1 Dongyun Qin,1 Chunjie Zhu1 1Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, and School of Pharmacy, Guangdong Medical University, Dongguan, 523808, People’s Republic of China; 2Medical College, Shaoguan University, Shaoguan, 512026, People’s Republic of China; 3Department of Biomedicine, Chengdu Medical College, Chengdu, 610500, People’s Republic of China*These authors contributed equally to this workCorrespondence: Manjiao Zhuang, Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, and School of Pharmacy, Guangdong Medical University, Dongguan, 523808, People’s Republic of China, Email manjiao@163.com Chunjie Zhu, School of Basic Medicine, Guangdong Medical University, Dongguan, 523808, People’s Republic of China, Email zhuchunjie718@163.comIntroduction: Quercetin has an ideal therapeutic effect on islet function improvement in type 2 diabetes mellitus (T2DM). However, the therapeutic benefit of quercetin is hindered by its poor bioavailability and limited concentration in pancreatic islets. In this study, superparamagnetic iron oxide nanoparticle (SPION)-modified exosomes were prepared to load quercetin, hoping to endow quercetin with enhanced water solubility and active targeting capacity with the help of magnetic force (MF).Methods: Transferrin-modified SPIONs (Tf-SPIONs) were synthesized by exploiting N-hydroxysuccinimidyl (NHS) conjugation chemistry, and quercetin-loaded exosomes (Qu-exosomes) were acquired by electroporation. Tf-SPION-modified quercetin-loaded exosomes (Qu-exosome-SPIONs) were generated by the self-assembly of transferrin (Tf) and the transferrin receptor (TfR). The solubility of quercetin was determined by high-performance liquid chromatography (HPLC) analysis. The pancreatic islet targeting capacity and insulin secretagogue and antiapoptotic activities of Qu-exosome-SPIONs/MF were evaluated both in vitro and in vivo.Results: The Qu-exosome-SPIONs were well constructed and harvested by magnetic separation with a uniform size and shape in a diameter of approximately 86.2 nm. The water solubility of quercetin increased 1.97-fold when loaded into the SPION-modified exosomes. The application of SPIONs/MF endowed the Qu-exosomes with favorable targeting capacity. In vitro studies showed that Qu-exosome-SPIONs/MF more effectively inhibited or attenuated β cell apoptosis and promoted insulin secretion in response to elevated glucose (GLC) compared with quercetin or Qu-exosome-SPIONs. In vivo studies demonstrated that Qu-exosome-SPIONs/MF displayed an ideal pancreatic islet targeting capacity, thereby leading to the restoration of islet function.Conclusion: The Qu-exosome-SPIONs/MF nano-delivery system significantly enhanced the quercetin concentration in pancreatic islets and thereby improved pancreatic islet protection.Keywords: quercetin, exosome, SPION, solubility, targeted delivery, islet function
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- 2023
7. Integrated Bioinformatics Analysis and Experimental Verification of Immune Cell Infiltration and the Related Core Genes in Ulcerative Colitis
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Zhao D, Qin D, Yin L, and Yang Q
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ulcerative colitis ,bioinformatics analysis ,core genes ,immune cells ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Danya Zhao,1 Danping Qin,2 Liming Yin,3 Qiang Yang4 1The First School of Clinical Medicine of Zhejiang Chinese Medical University, Hangzhou, People’s Republic of China; 2Department of Gastroenterology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, People’s Republic of China; 3Institute of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, People’s Republic of China; 4Department of Gastroenterology, Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, People’s Republic of ChinaCorrespondence: Danping Qin, Email qindp19841@sina.comBackground: Ulcerative colitis is a recurrent autoimmune disease. At present, the pathogenesis of UC is not completely clear. Hence, the etiology and underlying molecular mechanism need to be further investigated.Methods: Three sets of microarray datasets were included from the Gene Expression Omnibus database. The differentially expressed genes in two sets of datasets were analyzed using the R software, and the core genes of UC were screened using machine learning. The sensitivity and specificity of the core genes were evaluated with the receiver operating characteristic curve in another microarray dataset. Subsequently, the CIBERSORT tool was used to analyze the relationship between UC and its core genes and immune cell infiltration. To verify the relationship between UC and core genes and the relationship between core genes and immune cell infiltration in vivo.Results: A total of 36 DEGs were identified. AQP8, HMGCS2, and VNN1 were determined to be the core genes of UC. These genes had high sensitivity and specificity in receiver operating characteristic curve analysis. According to the analysis of immune cell infiltration, neutrophils, monocytes, and macrophages were positively correlated with UC. AQP8, HMGCS2, and VNN1 were also correlated with immune cell infiltration to varying degrees. In vivo experiments verified that the expressions of neutrophils, monocytes, and macrophages increased in the UC colon. Furthermore, the expressions of AQP8 and HMGCS2 decreased, whereas that of VNN1 increased. Azathioprine treatment improved all the indicators to different degrees.Conclusion: AQP8, HMGCS2, and VNN1 are the core genes of UC and exhibit different degrees of correlation with immune cells. These genes are expected to become new therapeutic targets for UC. Moreover, the occurrence and development of UC are influenced by immune cell infiltration.Keywords: ulcerative colitis, bioinformatics analysis, core genes, immune cells
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- 2023
8. Susceptibility of PCSK2 Polymorphism to Hirschsprung Disease in Southern Chinese Children
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Wang B, Fang W, Qin D, He Q, and Lan C
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hirschsprung's disease ,hscr ,single nucleotide polymorphism ,snp ,proprotein convertase subtilisin/kexin type 2 ,pcsk2 ,genetic susceptibility ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Bingtong Wang,* Wenlin Fang,* Dingjiang Qin,* Qiuming He, Chaoting Lan Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangdong Provincial Clinical Research Center for Child Health, Guangzhou, 510623, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chaoting Lan, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangdong Provincial Clinical Research Center for Child Health, Guangzhou, 510623, People’s Republic of China, Email 415879043@qq.comIntroduction: Hirschsprung’s disease (HSCR) is a developmental defect of the enteric nervous system (ENS), which is caused by abnormal development of enteric neural crest cells. Its occurrence is caused by genetic factors and environmental factors. It has been reported that single nucleotide polymorphisms (SNPs) of proprotein convertase subtilisin/kexin type 2 (PCSK2) gene are associated with HSCR. However, the correlation of HSCR in southern Chinese population is still unclear.Methods: We assessed the association of rs16998727 with HSCR susceptibility in southern Chinese children using TaqMan SNP genotyping analysis of 2943 samples, including 1470 HSCR patients and 1473 controls. The association test between rs16998727 and phenotypes was performed using multivariable logistic regression analysis.Results: We got an unexpected result, PCSK2 SNP rs16998727 was not significantly different from HSCR and its HSCR subtypes: S-HSCR (OR = 1.08, 95% IC: 0.93~1.27, P_adj = 0.3208), L-HSCR (OR = 1.07, 95% IC: 0.84~1.36, P_adj = 0.5958) and TCA (OR = 0.94, 95% IC: 0.61~1.47, P_adj = 0.8001).Conclusion: In summary, we report that rs16998727 (PCSK2 and OTOR) is not associated with the risk of HSCR in southern Chinese population.Keywords: Hirschsprung’s disease, HSCR, single nucleotide polymorphism, SNP, proprotein convertase subtilisin/kexin type 2, PCSK2, genetic susceptibility
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- 2023
9. Serum Amyloid a Predicts Prognosis and Chemotherapy Efficacy in Patients with Advanced Pancreatic Cancer
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Ding H, Yang Q, Mao Y, Qin D, Yao Z, Wang R, Qin T, and Li S
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advanced pancreatic cancer ,serum amyloid a ,chemotherapy ,prognosis ,biomarker. ,Pathology ,RB1-214 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Honglu Ding,1,* Qiuxia Yang,2,* Yize Mao,1,* Dailei Qin,1 Zehui Yao,1 Ruiqi Wang,1 Tao Qin,3 Shengping Li1 1Department of Pancreatobiliary Surgery, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou, People’s Republic of China; 2Department of Medical Imaging, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Guangzhou, People’s Republic of China; 3Department of Medical Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shengping Li, Department of Pancreatobiliary Surgery, Sun Yat-sen University Cancer Center, 651 Dongfeng Road E, Guangzhou, Guangdong, 510060, People’s Republic of China, Tel +86- 020-87341843, Email lishengp@mail.sysu.edu.cnPurpose: There is an urgent need to discover a predictive biomarker to help patients with advanced pancreatic cancer (APC) choose appropriate chemotherapy regimens. This study aimed to determine whether baseline serum amyloid A (SAA) levels were associated with overall survival (OS), progression-free survival (PFS), and treatment response in patients with APC received chemotherapy.Patients and Methods: This retrospective study included 268 patients with APC who received first-line chemotherapy at the Sun Yat-Sen University Cancer Center between January 2017 and December 2021. We examined the effect of baseline SAA on OS, PFS and chemotherapy response. The X-Tile program was used to determine the critical value for optimizing the significance of segmentation between Kaplan-Meier survival curves. The Kaplan-Meier curves and Cox regression analyses were used to analyze OS and PFS.Results: The best cut-off value of baseline SAA levels for OS stratification was 8.2 mg/L. Multivariate analyses showed that SAA was an independent predictor of OS (Hazard Ratio (HR) = 1.694, 95% Confidence Interval (CI) = 1.247– 2.301, p = 0.001) and PFS (HR = 1.555, 95% CI = 1.152– 2.098, p = 0.004). Low SAA was associated with longer OS (median, 15.7 months vs 10.0 months, p < 0.001) and PFS (median, 7.6 months vs 4.8 months, p < 0.001). The patients with a low SAA who received mFOLFIRINOX had longer OS (median, 28.5 months vs 15.1 months, p = 0.019) and PFS (median, 12.0 months vs 7.4 months, p = 0.035) than those who received nab-paclitaxel plus gemcitabine (AG) or SOXIRI, whereas there was no significant difference among the three chemotherapy regimens in patients with a high SAA.Conclusion: Owing to the rapid and simple analysis of peripheral blood, baseline SAA might be a useful clinical biomarker, not only as a prognostic biomarker for patients with APC, but also as a guide for the selection of chemotherapy regimens.Keywords: advanced pancreatic cancer, serum amyloid A, chemotherapy, prognosis, biomarker
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- 2023
10. Regulating bimetallic active centers for exploring the structure–activity relationship toward high-performance photocatalytic nitrogen reduction
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Zhang, W., Pan, G., Han, D., Liu, T., Liang, W., Dai, M., Xie, H., Qin, D., and Niu, L.
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- 2023
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11. Dietary Supplement of Anoectochilus roxburghii (Wall.) Lindl. Polysaccharides Ameliorates Cognitive Dysfunction Induced by High Fat Diet via “Gut-Brain” Axis
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Fu L, Zhu W, Tian D, Tang Y, Ye Y, Wei Q, Zhang C, Qiu W, Qin D, Yang X, and Huang Y
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anoectochilus roxburghii (wall.) lindl. polysaccharides ,diet-induced-obesity ,cognitive dysfunction ,gut microbiota ,“gut-brain” axis ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Liya Fu,1,2,* Wanlong Zhu,1,2,* Dongmei Tian,1,2 Yong Tang,2,3 Yun Ye,1 Qiming Wei,1 Chengbin Zhang,1 Wenqiao Qiu,2 Dalian Qin,2 Xuping Yang,1,2 Yilan Huang1,2 1Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of China; 2School of Pharmacy, Southwest Medical University, Luzhou, People’s Republic of China; 3State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, 999078, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xuping Yang; Yilan Huang, Department of Pharmacy, The Affiliated Hospital of Southwest Medical University, Luzhou, People’s Republic of China, Email yangxuping6552@163.com; lzyxyyxp123@163.comPurpose: Anoectochilus roxburghii (Wall.) Lindl. polysaccharides (ARPs) have been reported to exhibit multiple pharmacological activities including anti-inflammatory and anti-hyperglycemia. This study aims to investigate the effect of ARPs on cognitive dysfunction induced by high fat diet (HFD).Methods: Six-week-old male mice were treated with ARPs by dietary supplementation for 14 weeks. The effect of ARPs on cognitive function was determined by assessing the changes in spatial learning and memory ability, neurotrophic factors in hippocampus, inflammatory parameters, intestinal barrier integrity, and gut microbiota.Results: ARPs supplementation can effectively ameliorate cognitive dysfunction, decrease the phosphorylation levels of Tau protein in hippocampus. Meanwhile, the increased body weight, plasma glucose, total cholesterol, inflammatory factors induced by HFD were abolished by ARPs treatment. Furthermore, ARPs treatment restored the intestinal epithelial barrier as evidenced by upregulation of intestinal tight junction proteins. Additionally, ARPs supplementation significantly decreased the relative abundance of several bacteria genus such as Parabacteroides, which may play regulatory roles in cognitive function.Conclusion: These results suggest that ARPs might be a promising strategy for the treatment of cognitive dysfunction induced by HFD. Mechanistically, alleviation of cognitive dysfunction by ARPs might be associated with the “gut-brain” axis.Graphical Abstract: Keywords: Anoectochilus roxburghii (Wall.) Lindl. polysaccharides, diet-induced-obesity, cognitive dysfunction, gut microbiota, “gut-brain” axis
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- 2022
12. Characterization of Silver Nanoparticles Synthesized by Leaves of Lonicera japonica Thunb
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Zhang Y, Cui L, Lu Y, He J, Hussain H, Xie L, Sun X, Meng Z, Cao G, Qin D, and Wang D
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leaves of lonicera japonica thunb. ,llj-agno3-nps ,green approach ,antioxidant ,antibacterial ,anticancer ,Medicine (General) ,R5-920 - Abstract
Yu Zhang,1– 3,* Li Cui,2,* Yizeng Lu,4 Jixiang He,5 Hidayat Hussain,6 Lei Xie,4 Xuan Sun,2 Zhaoqing Meng,7 Guiyun Cao,7 Dawei Qin,1 Daijie Wang2,3 1School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People’s Republic of China; 2School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, People’s Republic of China; 3Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, 274000, People’s Republic of China; 4Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, 250102, People’s Republic of China; 5School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250353, People’s Republic of China; 6Department of Bioorganic Chemistry, Leibniz Institute of Plant BioChemistry, Halle, D-06120, Germany; 7Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan, 250103, People’s Republic of China*These authors contributed equally to this workCorrespondence: Dawei Qin, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People’s Republic of China, Tel/Fax +86 53189631208, Email qdw@qlu.edu.cn Daijie Wang, Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, 274000, People’s Republic of China, Tel +86 53182605319, Fax +86 53182964889, Email wangdaijie@qlu.edu.cnBackground: The leaves of L. japonica (LLJ) are widely used as medicine in China. It is rich in caffeoylquinic acids, flavonoids and iridoid glycosides and has strong reducing capacities. Therefore, it can be used as a green material to synthesize silver nanoparticles.Methods: LLJ was used as a reducing agent to produce the LLJ-mediated silver nanoparticles (LLJ-AgNPs). The structure and physicochemical properties of LLJ-AgNPs were characterized by ultraviolet spectroscopy (UV-Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray powder diffraction (XRD). Antioxidant activity of LLJ-AgNPs was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. Antibacterial activity was determined by 96 well plates (AGAR) gradient dilution, while the anticancer potential was determined by MTT assay.Results: The results showed LLJ-AgNPs had a spherical structure with the maximum UV-Vis absorption at 400 nm. In addition, LLJ-AgNPs exhibited excellent antioxidant properties, where the free radical scavenging rate of LLJ-AgNPs was increased from 39% to 92% at concentrations from 0.25 to 1.0 mg/mL. Moreover, LLJ-AgNPs displayed excellent antibacterial properties against E. coli and Salmonella at room temperature, with minimum inhibitory values of 10− 6 and 10− 5 g/L, respectively. In addition, the synthetic LLJ-AgNPs exhibited a better inhibition effect in the proliferation of cancer cells (HepG2, MDA-MB − 231, and Hela cells).Conclusion: The present study provides a green approach to synthesize LLJ-AgNPs. All those findings illustrated that the produced LLJ-AgNPs can be used as an economical and efficient functional material for further applications in food and pharmaceutical fields.Keywords: leaves of Lonicera japonica Thunb, LLJ-AgNO3-NPs, green approach, antioxidant, antibacterial, anticancer
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- 2022
13. Study on electromechanical coupled dynamic characteristics of transmission system of wind turbine
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Chen, R., primary, Qin, D., additional, and Liu, C., additional
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- 2022
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14. Living within the safe and just Earth system boundaries for blue water
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Stewart-Koster, B. Bunn, S. E. Green, P. Ndehedehe, C. Andersen, L. S. Armstrong McKay, D. I. Bai, X. DeClerck, F. Ebi, K. L. Gordon, C. Gupta, J. Hasan, S. Jacobson, L. Lade, S. J. Liverman, D. Loriani, S. Mohamed, A. Nakicenovic, N. Obura, D. Qin, D. Rammelt, C. Rocha, J. C. Rockström, J. Verburg, P. H. Zimm, C. and Stewart-Koster, B. Bunn, S. E. Green, P. Ndehedehe, C. Andersen, L. S. Armstrong McKay, D. I. Bai, X. DeClerck, F. Ebi, K. L. Gordon, C. Gupta, J. Hasan, S. Jacobson, L. Lade, S. J. Liverman, D. Loriani, S. Mohamed, A. Nakicenovic, N. Obura, D. Qin, D. Rammelt, C. Rocha, J. C. Rockström, J. Verburg, P. H. Zimm, C.
- Abstract
Safe and just Earth system boundaries (ESBs) for surface water and groundwater (blue water) have been defined for sustainable water management in the Anthropocene. Here we assessed whether minimum human needs could be met with surface water from within individual river basins alone and, where this is not possible, quantified how much groundwater would be required. Approximately 2.6 billion people live in river basins where groundwater is needed because they are already outside the surface water ESB or have insufficient surface water to meet human needs and the ESB. Approximately 1.4 billion people live in river basins where demand-side transformations would be required as they either exceed the surface water ESB or face a decline in groundwater recharge and cannot meet minimum needs within the ESB. A further 1.5 billion people live in river basins outside the ESB, with insufficient surface water to meet minimum needs, requiring both supply- and demand-side transformations. These results highlight the challenges and opportunities of meeting even basic human access needs to water and protecting aquatic ecosystems.
- Published
- 2024
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15. Spiking Neural Network Based Text Summarization System
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Hao, A. Xueyi, primary, Li, B. Yuze, additional, Lei Li, E., additional, Baofeng Li, C., additional, and Qin, D. Yu, additional
- Published
- 2023
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16. Investigation on large fluctuation transient process of high head Pelton turbine.
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Gao, J, Han, L, Wang, H J, Liu, Y X, Wei, X Z, and Qin, D Q
- Published
- 2024
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17. Unassisted Clinicians Versus Deep Learning-Assisted Clinicians in Image-Based Cancer Diagnostics:Systematic Review With Meta-analysis
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Xue, P., Si, M., Qin, D., Wei, B., Seery, S., Ye, Z., Chen, M., Wang, S., Song, C., Zhang, B., Ding, M., Zhang, W., Bai, A., Yan, H., Dang, L., Zhao, Y., Rezhake, R., Zhang, S., Qiao, Y., Qu, Y., Jiang, Y., Xue, P., Si, M., Qin, D., Wei, B., Seery, S., Ye, Z., Chen, M., Wang, S., Song, C., Zhang, B., Ding, M., Zhang, W., Bai, A., Yan, H., Dang, L., Zhao, Y., Rezhake, R., Zhang, S., Qiao, Y., Qu, Y., and Jiang, Y.
- Abstract
BACKGROUND: A number of publications have demonstrated that deep learning (DL) algorithms matched or outperformed clinicians in image-based cancer diagnostics, but these algorithms are frequently considered as opponents rather than partners. Despite the clinicians-in-the-loop DL approach having great potential, no study has systematically quantified the diagnostic accuracy of clinicians with and without the assistance of DL in image-based cancer identification. OBJECTIVE: We systematically quantified the diagnostic accuracy of clinicians with and without the assistance of DL in image-based cancer identification. METHODS: PubMed, Embase, IEEEXplore, and the Cochrane Library were searched for studies published between January 1, 2012, and December 7, 2021. Any type of study design was permitted that focused on comparing unassisted clinicians and DL-assisted clinicians in cancer identification using medical imaging. Studies using medical waveform-data graphics material and those investigating image segmentation rather than classification were excluded. Studies providing binary diagnostic accuracy data and contingency tables were included for further meta-analysis. Two subgroups were defined and analyzed, including cancer type and imaging modality. RESULTS: In total, 9796 studies were identified, of which 48 were deemed eligible for systematic review. Twenty-five of these studies made comparisons between unassisted clinicians and DL-assisted clinicians and provided sufficient data for statistical synthesis. We found a pooled sensitivity of 83% (95% CI 80%-86%) for unassisted clinicians and 88% (95% CI 86%-90%) for DL-assisted clinicians. Pooled specificity was 86% (95% CI 83%-88%) for unassisted clinicians and 88% (95% CI 85%-90%) for DL-assisted clinicians. The pooled sensitivity and specificity values for DL-assisted clinicians were higher than for unassisted clinicians, at ratios of 1.07 (95% CI 1.05-1.09) and 1.03 (95% CI 1.02-1.05), respectively. Similar diagnostic
- Published
- 2023
18. Living within the safe and just Earth system boundaries for blue water
- Author
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Stewart-Koster, B., Bunn, S.E., Green, P., Ndehedehe, C., Andersen, L., Armstrong McKay, D., Bai, X., DeClerck, F., Ebi, K., Gordon, C., Gupta, J., Hasan, S., Jacobson, L., Lade, S., Liverman, D., Loriani, S., Mohamed, A., Nakicenovic, N., Obura, D., Qin, D., Rammelt, C., Rocha, J., Rockström, J., Verburg, P., Zimm, C., Stewart-Koster, B., Bunn, S.E., Green, P., Ndehedehe, C., Andersen, L., Armstrong McKay, D., Bai, X., DeClerck, F., Ebi, K., Gordon, C., Gupta, J., Hasan, S., Jacobson, L., Lade, S., Liverman, D., Loriani, S., Mohamed, A., Nakicenovic, N., Obura, D., Qin, D., Rammelt, C., Rocha, J., Rockström, J., Verburg, P., and Zimm, C.
- Abstract
Safe and just Earth system boundaries (ESBs) for surface water and groundwater (blue water) have been defined for sustainable water management in the Anthropocene. Here we assessed whether minimum human needs could be met with surface water from within individual river basins alone and, where this is not possible, quantified how much groundwater would be required. Approximately 2.6 billion people live in river basins where groundwater is needed because they are already outside the surface water ESB or have insufficient surface water to meet human needs and the ESB. Approximately 1.4 billion people live in river basins where demand-side transformations would be required as they either exceed the surface water ESB or face a decline in groundwater recharge and cannot meet minimum needs within the ESB. A further 1.5 billion people live in river basins outside the ESB, with insufficient surface water to meet minimum needs, requiring both supply- and demand-side transformations. These results highlight the challenges and opportunities of meeting even basic human access needs to water and protecting aquatic ecosystems.
- Published
- 2023
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19. Safe and just Earth system boundaries
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Rockström, J., Gupta, J., Qin, D., Lade, S.J., Abrams, J.F., Andersen, L.S., Armstrong McKay, D.I., Bai, X., Bala, G., Bunn, S.E., Ciobanu, D., DeClerck, F., Ebi, K., Gifford, L., Gordon, C., Hasan, S., Kanie, N., Lenton, T.M., Loriani, S., Liverman, D.M., Mohamed, A., Nakicenovic, N., Obura, D., Ospina, D., Prodani, K., Rammelt, C., Sakschewski, B., Scholtens, J., Stewart-Koster, B., Tharammal, T., van Vuuren, D., Verburg, P.H., Winkelmann, R., Zimm, C., Bennett, E.M., Bringezu, S., Broadgate, W., Green, P.A., Huang, L., Jacobson, L., Ndehedehe, C., Pedde, S., Rocha, J., Scheffer, M., Schulte-Uebbing, L., de Vries, W., Xiao, C., Xu, C., Xu, X., Zafra-Calvo, N., Zhang, X., Rockström, J., Gupta, J., Qin, D., Lade, S.J., Abrams, J.F., Andersen, L.S., Armstrong McKay, D.I., Bai, X., Bala, G., Bunn, S.E., Ciobanu, D., DeClerck, F., Ebi, K., Gifford, L., Gordon, C., Hasan, S., Kanie, N., Lenton, T.M., Loriani, S., Liverman, D.M., Mohamed, A., Nakicenovic, N., Obura, D., Ospina, D., Prodani, K., Rammelt, C., Sakschewski, B., Scholtens, J., Stewart-Koster, B., Tharammal, T., van Vuuren, D., Verburg, P.H., Winkelmann, R., Zimm, C., Bennett, E.M., Bringezu, S., Broadgate, W., Green, P.A., Huang, L., Jacobson, L., Ndehedehe, C., Pedde, S., Rocha, J., Scheffer, M., Schulte-Uebbing, L., de Vries, W., Xiao, C., Xu, C., Xu, X., Zafra-Calvo, N., and Zhang, X.
- Abstract
The stability and resilience of the Earth system and human well-being are inseparably linked1,2,3, yet their interdependencies are generally under-recognized; consequently, they are often treated independently4,5. Here, we use modelling and literature assessment to quantify safe and just Earth system boundaries (ESBs) for climate, the biosphere, water and nutrient cycles, and aerosols at global and subglobal scales. We propose ESBs for maintaining the resilience and stability of the Earth system (safe ESBs) and minimizing exposure to significant harm to humans from Earth system change (a necessary but not sufficient condition for justice)4. The stricter of the safe or just boundaries sets the integrated safe and just ESB. Our findings show that justice considerations constrain the integrated ESBs more than safety considerations for climate and atmospheric aerosol loading. Seven of eight globally quantified safe and just ESBs and at least two regional safe and just ESBs in over half of global land area are already exceeded. We propose that our assessment provides a quantitative foundation for safeguarding the global commons for all people now and into the future.
- Published
- 2023
20. Exploration and Application of Cloud-Network Integration Control Architecture in Industry.
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Sun, R Q, Zhang, Y, Liu, L, Qin, D W, Liu, J W, and Wu, X N
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- 2023
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21. STUDY ON THE SPATIAL-TEMPORAL EVOLUTION OF SUBSIDENCE IN THE BEIBU GULF REGION BY TIME-SERIES INSAR
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Wang, C., primary, Wei, B., additional, Zhou, L., additional, Qin, D., additional, Zhao, Y., additional, and Li, J., additional
- Published
- 2022
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22. UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair
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Huang, Q., Qin, D., Pei, D., Vermeulen, M., Zhang, X., Huang, Q., Qin, D., Pei, D., Vermeulen, M., and Zhang, X.
- Abstract
Contains fulltext : 243889.pdf (Publisher’s version ) (Open Access)
- Published
- 2022
23. Age of Himalayan bottom ice cores [2]
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Hou, S. G., Qin, D. H., Jouzel, J., Masson-Delmotte, V., Von Grafenstein, U., Landais, A., Caillon, N., Chappellaz, J., Hou, S. G., Qin, D. H., Jouzel, J., Masson-Delmotte, V., Von Grafenstein, U., Landais, A., Caillon, N., and Chappellaz, J.
- Published
- 2022
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24. The liquid Kangfuxin (KFX) has efficient antifungal activity and can be used in the treatment of vulvovaginal candidiasis in mice
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Ma, J., primary, Yang, Y.-C., additional, Su, L.-Q., additional, Qin, D.-M., additional, Yuan, K., additional, Zhang, Y., additional, and Wang, R.-R., additional
- Published
- 2022
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25. Urban traffic safety situation assessment method based on the data of internet of vehicles.
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Wang, Y. H., Song, M. L., Li, A. Z., Qin, D. D., and Zhao, T.
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TRAFFIC safety ,CITY traffic ,ANALYTIC hierarchy process ,DATABASES ,TRAFFIC accidents ,URBAN transportation ,INTERNET - Abstract
Traffic safety situation refers to the current traffic operation safety situation and future evolution trend of one or more related highways. Accurate traffic safety situation assessment is of great significance for promoting traffic safety. This paper proposes a new urban traffic safety situation evaluation method based on Internet of Vehicles data. First, based on vehicle data, traffic condition data is collected as sample data for urban traffic safety assessment. Secondly, from the perspective of traffic environment, traffic characteristics, road traffic accidents and road conditions, a traffic safety evaluation index system is constructed and quantified. Finally, the weight of each index is calculated by using the analytic hierarchy process, and the evaluation score of urban traffic safety is calculated to judge the safety level. The experimental results show that, compared with the traditional traffic safety situation assessment method, the evaluation accuracy and efficiency of this method have been greatly improved, and the evaluation accuracy rate is more than 95%. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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26. On the Scale Size of the Aerodynamic Characteristics of a High-Speed Train.
- Author
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Chang, C., Li, T., Qin, D., and Zhang, J.
- Subjects
COMPUTATIONAL fluid dynamics ,LIFT (Aerodynamics) ,HIGH speed trains ,REYNOLDS number ,WIND tunnel testing ,DRAG (Aerodynamics) ,FLOW separation ,DRAG coefficient - Abstract
In the wind tunnel test of trains, the scale size changes the Reynolds numbers of trains, which may affect the aerodynamic characteristics of the train. Based on computational fluid dynamics (CFD), numerical models of train aerodynamics with five different scale sizes are established. The five different scale sizes are λ=1/1, 1/2, 1/8, 1/16 and 1/25, respectively, and the aerodynamic characteristics of trains running in the open-air operating condition and crosswind operating condition with different scale sizes are numerically simulated. The results show that the pressure drag coefficients and pressure lift coefficients of the train tend to decrease with the decrease of the scale size. In the open-air operating condition, compared with the full-size train, the pressure drag coefficient of the 1/25
th scaled train is less by 14.4%, and the pressure lift coefficients of the head car, middle car and tail car change 16.1%, 46.6% and 12.3%, respectively. The scale size affects the velocity gradient near the train surface and the position of flow separation changes. The decrease of the scale size leads to the decrease of Reynolds numbers and the increase of viscous drag coefficient. When the scale size is 1/25, the viscous drag coefficient of the train is 0.186, which is 48.6% larger than the one of the full-size train. Compared with the open-air operating condition, the trend of the pressure drag coefficients and viscous drag coefficients is consistent except for the head car in crosswind operating condition when the scale size decreases. In the range of scale size λ between 1/1 and 1/25, the aerodynamic drag coefficient of the head car, middle car and tail car increase with the decrease of scale size, and the difference in the aerodynamic drag coefficient of the train is 12.9%. In addition, the train's aerodynamic lift coefficient shows an increasing trend with the decrease of scale size. [ABSTRACT FROM AUTHOR]- Published
- 2022
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27. Asymptomatic cement leakage into inferior vena cava.
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Zhao, Z, Qin, D, and Zhao, W
- Subjects
- *
VENA cava inferior , *VERTEBRAE injuries , *CEMENT , *LEAKAGE - Published
- 2022
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28. Yiqi Yangxue formula inhibits cartilage degeneration in knee osteoarthritis by regulating LncRNA-UFC1/miR-34a/MMP-13 axis.
- Author
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Zhao T, Wang X, Li Z, and Qin D
- Subjects
- Animals, Rats, Male, Chondrocytes drug effects, Chondrocytes metabolism, Cartilage, Articular drug effects, Cartilage, Articular pathology, Cartilage, Articular metabolism, Disease Models, Animal, Matrix Metalloproteinase 13 metabolism, Matrix Metalloproteinase 13 genetics, MicroRNAs metabolism, MicroRNAs genetics, Drugs, Chinese Herbal pharmacology, Rats, Sprague-Dawley, RNA, Long Noncoding genetics, RNA, Long Noncoding metabolism, Osteoarthritis, Knee drug therapy, Osteoarthritis, Knee pathology, Osteoarthritis, Knee metabolism
- Abstract
Ethnopharmacological Relevance: Knee osteoarthritis (KOA) is a prevalent and disabling clinical condition affecting joint structures worldwide. The Yiqi Yangxue formula (YQYXF) is frequently prescribed in clinical settings for the treatment of KOA. Existing research has primarily focused on alterations in drug metabolism, with limited investigation into the epigenetic effects of YQYXF, particularly in relation to non-coding RNA., Aim of the Study: Exploring the effects of YQYXF on critical factors of long chain non-coding RNA UFC1/miR-34a/matrix metalloproteinase-13 (MMP-13) axis and their interrelationships., Methods: UHPLC-QE-MS technology was used to identify the YQYXF ingredients in rat serum. KEGG and GO analysis were performed on the targets of blood components acting on KOA using a database. Simultaneously, a protein interaction network was constructed using target proteins and metabolites to identify the core components and key pathways of YQYXF. The KOA rat model was established using an improved Hulth method. SPF SD rats were randomly divided into normal group, sham surgery group, model group, celecoxib capsules group (18 mg/kg), YQYXF low, medium and high dose groups (4.6 g/kg, 9.2 g/kg, 18.4 g/kg). Observe the synovial and cartilage tissues of rats using pathological methods. RT-PCR was used to detect the levels of UFC1, miR-34a, and MMP-13 in cartilage. Immunohistochemistry was used to detect the levels of MMP-13 and ADAMTS-5 in cartilage. ELISA method was used to detect the levels of MMP-13 and ADAMTS-5 in serum. In addition, we further validated the regulation of crucial factor expression levels of UFC1/miR-34a/MMP-13 axis in rat chondrocytes and degenerative chondrocytes of KOA patients by YQYXF, providing a basis for its treatment of KOA., Results: The compounds that YQYXF enters the bloodstream mainly contain flavonoids and phenylpropanoid compounds. The core components that act on OA include quercetin, fisetin, and demethylweldelolactone. The main target pathways are the IL-17 signaling pathway, lipid and atherosclerosis, cellular sensitivity, inflammatory mediator regulation of TRP channels, TNF signaling pathway, relaxin signaling pathway and C-type lectin receptor signaling pathway. YQYXF inhibited the expression of miR-34a and MMP-13 mRNA, and reduced the protein levels of MMP-13 and ADAMTS-5. In vitro studies have confirmed that 20% YQYXF serum promoted UFC1 and reduce miR-34a levels. In addition, miR-34a in sh-UFC1+10% YQYXF serum and sh-UFC1+20% YQYXF serum groups significantly decreased compared to the sh-UFC1 group., Conclusion: The anti-KOA cartilage degeneration effect of YQYXF might be related to inhibiting cell apoptosis and promoting cell proliferation, which regulated the lncRNA-UFC1/miR-34a/MMP-13 axis., Competing Interests: Declaration of competing interest No potential conflict of interest was reported by the authors., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2025
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29. c-JUN: a chromatin repressor that limits mesoderm differentiation in human pluripotent stem cells.
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Zhang R, Li G, Zhang Q, Wang Z, Xiang D, Zhang X, Chen J, Hutchins AP, Qin D, Su H, Pei D, and Li D
- Subjects
- Humans, GATA4 Transcription Factor metabolism, GATA4 Transcription Factor genetics, DNA-Binding Proteins metabolism, DNA-Binding Proteins genetics, Mi-2 Nucleosome Remodeling and Deacetylase Complex metabolism, Mi-2 Nucleosome Remodeling and Deacetylase Complex genetics, Transcription Factors metabolism, Transcription Factors genetics, Cell Line, Gene Expression Regulation, Developmental, Cell Lineage genetics, Mesoderm cytology, Mesoderm metabolism, Cell Differentiation genetics, Chromatin metabolism, Pluripotent Stem Cells metabolism, Pluripotent Stem Cells cytology, Proto-Oncogene Proteins c-jun metabolism, Proto-Oncogene Proteins c-jun genetics, T-Box Domain Proteins genetics, T-Box Domain Proteins metabolism
- Abstract
Cell fate determination at the chromatin level is not fully comprehended. Here, we report that c-JUN acts on chromatin loci to limit mesoderm cell fate specification as cells exit pluripotency. Although c-JUN is widely expressed across various cell types in early embryogenesis, it is not essential for maintaining pluripotency. Instead, it functions as a repressor to constrain mesoderm development while having a negligible impact on ectoderm differentiation. c-JUN interacts with MBD3-NuRD complex, which helps maintain chromatin in a low accessibility state at mesoderm-related genes during the differentiation of human pluripotent stem cells into mesoderm. Furthermore, c-JUN specifically inhibits the activation of key mesoderm factors, such as EOMES and GATA4. Knocking out c-JUN or inhibiting it with a JNK inhibitor can alleviate this suppression, promoting mesoderm cell differentiation. Consistently, knockdown of MBD3 enhances mesoderm generation, whereas MBD3 overexpression impedes it. Overexpressing c-JUN redirects differentiation toward a fibroblast-like lineage. Collectively, our findings suggest that c-JUN acts as a chromatin regulator to restrict the mesoderm cell fate., (© The Author(s) 2025. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Published
- 2025
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30. Comparative miRNAome combined with transcriptome and degradome analysis reveals a novel miRNA-mRNA regulatory network associated with starch metabolism affecting pre-harvest sprouting resistance in wheat.
- Author
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Sun L, Zhao T, Qin D, Dong J, Zhang D, and Ren X
- Subjects
- Gene Expression Regulation, Plant, Gene Expression Profiling, Seeds genetics, Seeds growth & development, Triticum genetics, MicroRNAs genetics, Germination genetics, Starch metabolism, Transcriptome, Gene Regulatory Networks, RNA, Messenger genetics, RNA, Messenger metabolism, RNA, Plant genetics
- Abstract
Background: Pre-harvest sprouting (PHS) is one of the most important problems associated with the severe decrease of yield and quality under disaster weather of continuous rain in wheat harvesting stage. At present, the functions and mechanisms related to the involvement of post-transcriptional regulation has not been studied very clearly in PHS resistance., Results: This study compared the differences of germinated seeds in miRNAome between the PHS-tolerant and PHS-susceptible white wheat varieties. A total of 1879 miRNAs were identified from three different stages during seed germination. In order to further obtain candidate miRNAs, the different datasets of differentially expressed miRNAs were excavated by using differential-expression and time-series analysis. Combined with degradome data, the miRNA-mRNA networks analysis was performed after genome-wide screening of target genes, and then KEGG enrichment highlighted that the starch and sucrose metabolism pathway related to PHS was specifically enriched in an especial target-gene dataset derived from R12R18-HE miRNAs. Based on transcriptome data, a network associated with starch metabolism was systematically and completely reconstructed in wheat. Then, the starch degradation pathway controlled by seven miRNA-RNA pairs were supposed to be the essential regulation center for seed germination in wheat, which also could play a critical role on the PHS resistance., Conclusion: Our findings revealed the complex impact of the miRNA-mediated mechanism for forming intrinsic and inherent differences, which resulting in significant difference on PHS performance between white wheat varieties., Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)
- Published
- 2025
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31. Vinculin: a new target for the diagnosis and treatment of disease.
- Author
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Li J, Wang W, Lin Z, Liu Z, Zhang R, Li R, Zhang J, Zheng Y, Qin D, Wu Y, and Liu Y
- Abstract
Vinculin, a crucial adhesion plaque protein, plays a significant role in cell morphology and tissue development. Dysregulation of focal adhesion proteins has been linked to numerous diseases, including cardiovascular conditions, gastrointestinal disorders, and cancer. Recent studies increasingly highlight vinculin's involvement in the progression of these diseases; however, a comprehensive review remains lacking. Therefore, an in-depth and timely review is essential to consolidate the latest findings on vinculin's role in disease mechanisms. This study aims to examine how vinculin coordinates a complex network of signaling pathways across various pathological contexts., Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025. Published by Elsevier Ltd.)
- Published
- 2025
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32. Associations Between Prenatal Dioxin-like Polychlorinated Biphenyls Exposure and Glucocorticoid and Androgenic Hormones in Umbilical Cord Blood.
- Author
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She M, Lv X, Chu S, Wang R, and Qin D
- Abstract
This study investigates the association between prenatal exposure to dioxin-like polychlorinated biphenyls (DL-PCBs) and glucocorticoid and androgenic hormone levels in cord blood. We analyzed cord blood samples from 500 mother-infant pairs from China (2022-2023), focusing on hormones including cortisol, cortisone, dehydroepiandrosterone (DHEA), and androstenedione. The main analysis revealed significant reductions in cortisol levels with increased exposure to PCB-77 (β = -3.37, 95% CI: -6.41, -0.32), PCB-81 (β = -2.90, 95% CI: -5.40, -0.39), and PCB-105 (β = -2.27, 95% CI: -4.45, -0.09). Cortisone levels also decreased with PCB-77 (β = -6.00, 95% CI: -10.98, -1.02), while DHEA increased with PCB-81 exposure (β = 0.19, 95% CI: 0.08, 0.31). Furthermore, the cortisol/DHEA ratio decreased significantly with PCB-77 (β = -2.45, 95% CI: -4.01, -0.88), indicating a disruption in the balance of glucocorticoid and androgenic hormones. Stratified analyses revealed significant sex-specific associations. Among boys, PCB189 was most strongly associated with reduced cortisol levels, while PCB169 exhibited the largest negative effect in girls. Mixture analysis using quantile g-computation demonstrated that each quartile increase in combined DL-PCB exposure was associated with a significant decrease in cortisol (β = -2.85, 95% CI: -4.73, -0.97) and cortisone (β = -4.78, 95% CI: -7.85, -1.71), alongside a significant increase in DHEA (β = 0.27, 95% CI: 0.11, 0.43) and androstenedione (β = 0.02, 95% 0.01, 0.04). The cortisol/DHEA and glucocorticoid/androgenic ratios also showed a significant reduction. Generalized weighted quantile sum (gWQS) analysis corroborated the negative associations for cortisol and cortisone but did not detect significant effects for DHEA and androstenedione. These results underscore the complex interactions of DL-PCB exposure with glucocorticoid and androgenic hormones, highlighting the importance of sex-specific and mixture-based approaches to understanding endocrine disruption in prenatal development., Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025. Published by Elsevier Inc.)
- Published
- 2025
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33. Preparation of Ethosome Gel with Total Flavonoids from Vernonia anthelmintica (L.) Willd. for the Treatment of Vitiligo.
- Author
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Qin D, Cui Y, Zheng M, Yang Z, and Wang X
- Abstract
Vernonia anthelmintica (L.) Willd. is a traditional medicinal herb in Chinese medicine, extensively used by various ethnic groups due to the numerous advantages derived from its total flavonoids. These benefits encompass anti-inflammatory and antioxidant effects, and the promotion of melanin production, showcasing its significant efficacy in addressing vitiligo. To improve transdermal absorption and enhance the antioxidant effectiveness of the treatment, ethosome containing total flavonoids were prepared utilizing the ultrasound injection technique. The resulting ethosome was then carefully mixed with 0.7% Carbomer 934 gel in equal parts, yielding a gel concentration of 0.302 mg/g. This formulation produced small, consistent ethosome that exhibited high encapsulation efficiency and notable stability. In vitro analyses demonstrated sustained release characteristics of the gel and considerable therapeutic effectiveness against vitiligo resulting from hydroquinone exposure. Histological examinations performed through hematoxylin and eosin (H&E) staining of mouse skin revealed increased melanin production and increased activities of tyrosinase (TYR), cholinesterase (CHE), and mouse monoamine oxidase (MAO), while levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were reduced. These findings underscore the promising effectiveness of this treatment strategy and validate the efficacy of the dosage form.
- Published
- 2025
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34. Residual, Enrichment and Health Risk Assessment of Hexachlorocyclohexane and Dichlorodiphenyltrichloroethane in Muscle of Cultured Common Carp.
- Author
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Huang L, Gao L, Wu S, Wang P, Chen Z, Hao Q, Qin D, and Huang X
- Abstract
Common carp ( Cyprinus carpio ) is extensively cultured and widely consumed in Heilongjiang Province, China. Due to the proximity of freshwater ponds to agricultural cultivated areas, these aquatic systems are inevitably influenced by the historical application of organochlorine pesticides (OCPs), due to their prolonged half-life and resistance to degradation. Gas chromatography-tandem mass spectrometry (GC-MS/MS) was utilized to quantify the levels of hexachlorocyclohexane (HCHs) and dichlorodiphenyltrichloroethane (DDTs) in the muscle tissue of cultured common carp. This study examined the enrichment characteristics of HCHs and DDTs in fish muscle, as well as their correlation with sediment and associated risk assessments. The results showed that the residual levels of HCHs and DDTs in fish muscle ranged from 0.387 μg·kg
-1 to 3.418 μg·kg-1 and from 0.114 μg·kg-1 to 0.420 μg·kg-1 , respectively. They were all below the maximum residue limits specified in GB 2763-2021 (HCHs: 100 μg·kg-1 , DDTs: 500 μg·kg-1 ). The concentrations of HCHs and DDTs in sediment were found to be lower than those in muscle tissue. Notably, the concentrations of HCHs were higher than those of DDTs in both muscle and sediment. Among the HCHs, γ-HCH was the predominant residual substance, contributing a significant proportion of 42.16% to 86.47%. No significant residues of DDT derivatives were detected. A significant correlation was observed between the concentrations of HCHs and DDTs in the muscle tissue and those present in the sediment ( p < 0.01). The health risk assessment indicated that both the carcinogenic and non-carcinogenic risks associated with OCPs from common carp muscle and sediment were within acceptable limits. Therefore, it was recommended to regulate fish consumption during the breeding period.- Published
- 2025
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35. Two Alkaline Metal Borates: from Layer to Layer-Pillared Framework.
- Author
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Chen J, Qin D, and Yang GY
- Abstract
Two borates, Na
2 K[B5 O8 (OH)2 ]·H2 O ( 1 ) and Na4 K2 [{B5 O8 }2 {B3 O5 }{BO2 (OH)2 }]·2H2 O ( 2 ) have been designed and made under solvothermal conditions. Compound 1 exhibits a 2D fluctuant layer based on the [B5 O10 (OH)2 ]7- clusters, containing two types of 9-membered ring (MR) channels and showing a four-connected sql topology net. By modifying the reactants and reaction temperature, compound 2 was obtained from compound 1 . The addition of boron-containing components not only increased their concentration but also enriched the B-O fragment while higher temperatures promoted greater condensation of the anionic clusters. Compound 2 crystallizes in the acentric space group Pc and is composed of two types of [B5 O11 ]7- clusters, [B3 O7 ]5- clusters and [BO2 (OH)2 ]3- tetrahedra. The two kinds of [B5 O11 ]7- clusters form a 2D layer similar to 1 , which is further connected by the chain built from the [B3 O7 ]5- clusters and decorated by [BO2 (OH)2 ]3- groups to produce a 3D layer-pillared framework. Notably, compound 2 is the first layer-pillared borate containing three types of B-O clusters and six types of channels. Second harmonic generation (SHG) measurements reveal that compound 2 displays a moderate SHG response of about 1.1 times that of KH2 PO4 (KDP).- Published
- 2025
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36. Discovering the compatibility of Coptidis Rhizoma and Gardeniae Fructus in attenuating hepatotoxicity through the association rules analysis of related formula.
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Yan X, Qin D, Zhang W, Ren Y, Lyu Y, Ma J, and Yu Q
- Abstract
Ethnopharmacological Relevance: Gardenia jasminoides J. Ellis (Gardeniae Fructus, GF) is a widely used herbal medicine in many prescriptions. However, inappropriate application of GF may induce hepatotoxicity, which greatly challenges its clinical application. In the field of Traditional Chinese Medicine, the principle of "Compatibility for Toxicity Attenuation" is a pivotal concept. So far, the basis and mechanisms of compatibility with GF remain unclear., Aims of the Study: We aimed to investigate the toxicity attenuating effects and potential mechanisms of compatibility of Coptis chinensis Franch (Coptidis Rhizoma, CR) and GF in hepatotoxicity., Methods and Results: Association rules analysis was performed in 817 GF-related formulas, and 49 associated items were selected, among which CR and GF were ranked in the top two. Network pharmacology was used to elucidate the potential mechanisms of combination of CR and GF in mitigating hepatotoxicity. Nine potential hepatotoxic components of GF and 14 active components of CR were focused on, and 9 common targets for CR and GF were identified. Further GO and KEGG analyses showed that the toxicity attenuating effect of CR on GF-induced hepatotoxicity may be closely correlated with inflammatory response, response to hypoxia, cancer signaling pathways, PI3K-Akt and HIF-1 signaling pathway. The in vitro results indicated that the combined use of CR and GF increased the viability of HepG2 cells. Furthermore, the in vivo data demonstrated that CR inhibited GF-induced increase in serum ALT and AST levels and pathological changes in the livers of KM mice. Besides, CR reduced the accumulation of ROS and MDA, inhibited the release of TNF-α and IL-6, while elevated GSH level in the mouse liver tissues. Finally, the molecular docking results indicated that the active components of CR had strong binding affinity with the monooxygenase cytochrome P450 3A4 (CYP3A4). CR combination restored the expression of CYP3A4 in the liver tissues of mice challenged with GF., Conclusion: Co-administration with CR effectively reduced GF-induced hepatotoxicity through alleviating oxidative damage, inflammatory response and enhancing CYP3A4 expression., Competing Interests: Declaration of competing interest There are no conflicts of interest among the authors participating in this study., (Copyright © 2025 Elsevier B.V. All rights reserved.)
- Published
- 2025
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37. Study of the SPL gene family and miR156-SPL module in Populus tomentosa: Potential roles in juvenile-to-adult phase transition and reproductive phase.
- Author
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Li Y, Deng Y, Qin D, and An X
- Abstract
Populus tomentosa, a deciduous tree species distinguished by its significant economic and ecological value, enjoys a wide-ranging natural distribution. However, its long juvenile period severely restricts the advancement of breeding work. The SPL gene family, a distinctive class of transcription factors exclusive to the plant kingdom, is critical in various processes of plant growth and development. The miR156-SPL molecular module stands as an indispensable regulatory mechanism in the transition from the vegetative juvenile phase to the adult phase in plants. Consequently, this research endeavored a methodical and exhaustive exploration of the SPL gene family within the P.tomentosa species, synergistically integrating the miR156 family into the analysis. A total of 56 PtSPL genes were identified and subjected to a comprehensive analysis of their gene structure, conserved motifs, collinearity relationships, chromosomal localization, and promoter cis-acting elements. Further analysis of gene expression profiles confirmed the pivotal role of PtSPLs in the reproductive phase and tissue development of P. tomentosa. In addition, 11 members of miR156 in P. tomentosa were identified and their sequences analyzed, elucidating the miR156-SPL regulatory network. The target relationship between miR156k and PtSPLs was further validated by detecting the expression levels of PtSPLs in transgenic poplars overexpressing 35S::MIR156k. This comprehensive study lays a robust theoretical foundation for the continued exploration and application of the SPL genes in P. tomentosa, opening avenues for future research and potential advancements in plant biology and breeding strategies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2025. Published by Elsevier B.V.)
- Published
- 2025
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38. Convergent Isobilateral Leaves Increase the Risk for Mangroves Facing Human-Induced Rapid Environmental Changes.
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Weng Y, Qin D, Li X, Zhou J, Zhang B, and Li QQ
- Abstract
Understanding plant adaptations in extreme environments is crucial, as these adaptations often confer advantages for survival. However, a significant gap exists regarding the genetic mechanisms underlying these adaptations and their responses to human-induced rapid environmental change (HIREC). This study addresses the question of whether genetic convergence occurs among plants with similar adaptive features, specifically focusing on isobilateral leaves in mangrove species. Here, we analyse the genetic convergence of isobilateral leaves in mangroves that have independently adapted to coastal intertidal zones. Our findings reveal that genetic convergence is evident in gene families involved in leaf abaxial and adaxial development, with strong selection pressures identified in photosynthesis and leaf polarity pathways. Despite these adaptations, mangrove species with isobilateral leaves occupy narrower ecological niches and face diminishing suitable habitat areas projected under various HIREC scenarios. These results indicate that while convergent traits enhance local adaptation, they may also increase vulnerability to ongoing environmental changes. This research provides valuable insight into the interplay between genetic adaptation and environmental resilience, underscoring the necessity for targeted biodiversity conservation strategies that safeguard specific adaptive traits amid rapid environmental shifts., (© 2025 John Wiley & Sons Ltd.)
- Published
- 2025
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39. Association between balance impairment and incidence of motoric cognitive risk syndrome in the China Health and Retirement Longitudinal Study.
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Xu Z, Jia S, Huang N, Ma Y, Qin D, and Dong B
- Abstract
Objectives: Motor cognitive risk (MCR) syndrome, defined as the cooccurrence of subjective cognitive complaints and a slow gait speed, is a form of pre-dementia condition. Balance has previously been associated with cognitive function. However, to date, no study has examined the relationship between balance and MCR in a large cohort of older adults. We aimed to investigate the associations of balance with MCR among Chinese older adults., Research Design and Methods: Data from the wave 1 to wave 3 of the China Health and Retirement Longitudinal Study (CHARLS) were used. Balance was measured using validated tandem stance. Logistic and discrete-time survival cox regression analyses were performed to examine the relationship between baseline balance impairment and prevalent and incident MCR., Results: A total of 3,398 participants were included in the baseline study. The prevalence of balance impairment was 21.1%. In the cross-sectional analysis, balance impairment was significantly associated with higher odds of MCR in the fully-adjusted model (OR: 1.43 95%CI 1.14-1.80, p = 0.002). A total of 2,474 individuals were included in the longitudinal analysis. During a mean follow-up duration of 3.69 years, the incidence of MCR was 9.8%. Baseline balance impairment was also significantly related to incidence of MCR (HR:1.37 95%CI 1.03-1.82, p = 0.032) even adjusting all confounders., Conclusion: These results show that early recognition of balance disorder may be helpful in the prevention and treatment of cognitive decline in older adults., Competing Interests: Declaration of competing interest We, the authors, declare that there are no conflicts of interest regarding the publication of this paper. We confirm that the work described in this manuscript has not been published before, and it is not under consideration for publication elsewhere. We also confirm that all authors have read and approved the manuscript and that there are no known conflicts of interest associated with this publication., (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2025
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40. Seasonal Variations in the Structure and Function of the Gut Flora in Adult Male Rhesus Macaques Reared in Outdoor Colonies.
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Lv L, Zhang F, Zhou H, Xiao W, Hu Y, Wang W, Zhu Z, Zhu F, Qin D, and Hu X
- Abstract
The seasonal variations that occur in the gut microbiota of healthy adult rhesus monkeys kept in outdoor groups under conventional rearing patterns and how these variations are affected by environmental variables are relatively poorly understood. In this study, we collected 120 fecal samples from 30 adult male rhesus monkeys kept in outdoor groups across four seasons and recorded the temperature and humidity of the housing facilities, as well as the proportions of fruit and vegetables in their diet. A 16S rRNA sequencing analysis showed that the alpha diversity of the gut microbiota of the rhesus monkeys was higher in winter and spring than in summer and autumn. A principal coordinate analysis (PCoA) further demonstrated notable seasonal variations in the composition and functionality of the gut microbiota in the rhesus monkeys. The phyla Firmicutes and Bacteroidetes and the genus Prevotella 9 were the significantly dominant groups in all 120 fecal samples from the rhesus monkeys. A linear discriminant analysis (LDA) effect size (LEfSe) analysis (LDA > 4) indicated that at the phylum level, Firmicutes was significantly enriched in winter, Bacteroidetes was significantly enriched in summer, and Proteobacteria and Campylobacter were significantly enriched in spring. At the genus level, Helicobacter and Ralstonia were significantly enriched in spring; Prevotella 9 , Streptococcus , and Prevotella were significantly enriched in summer; and UCG_005 was significantly enriched in autumn. The beneficial genera Lactobacillus , Limosilactobacillus , and Ligilactobacillus and the beneficial species Lactobacillus johnsonii , Limosilactobacillus reuteri , Ligilactobacillus murinus , and Lactobacillus amylovorus all showed the same seasonal trend; namely, their average relative abundance was markedly greater during the winter months compared to other seasons. Compared with other seasons, carbohydrate metabolic function was significantly upregulated in winter ( p < 0.01), amino acid metabolic function was relatively increased in spring, and energy metabolic function and the metabolic function of cofactors and vitamins were significantly downregulated in winter and relatively upregulated in summer. A variance partitioning analysis (VPA) and redundancy analysis (RDA) showed that the proportions of fruits and vegetables in the diet, but not climatic factors (temperature and humidity), significantly influenced the seasonal changes in the gut microbiota. These variations were related to changes in the proportions of fruits and vegetables. This research presents novel findings regarding the influence of external environmental factors on the gastrointestinal environment of rhesus monkeys.
- Published
- 2025
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41. Incorporation of anthocyanin into zein nanofibrous films by electrospinning: Structural characterization, functional properties, and ammonia color-responsiveness.
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Wang J, Zhao W, Qin D, Shan H, Zhu X, Chen L, Liu Y, and Li S
- Abstract
Green electrospinning for the production of freshness-indicating labels, employing entirely natural biopolymers and pigments, holds significance in the development of intelligent food packaging. This study aimed to prepare zein (Z) fibrous film (FF) incorporated with varying concentrations of anthocyanin (A; 0-0.5 %) through green electrospinning. Furthermore, we evaluated their structural characteristics, functional properties, and color responsiveness to ammonia. With an increase in A concentration from 0 % to 0.5 %, the average thickness and fiber diameter of the electrospun Z-FF increased from 68.6 μm to 102.0 μm and from 582.1 nm to 690.7 nm, respectively. At A concentration of 0.2 %, the A content and loading efficiency of electrospun ZA-FF exhibited the most optimal values (6.8 mg/g and 39.4 %). Surface hydrophobicity, thermodynamic properties, and mechanical characteristics of ZA-FF were significantly improved, attributable to changes in their secondary and crystal structures from the interaction of Z and A. In the ammonia color-responsiveness test, an obvious color change with Δ E values of 13.1 and 20.0 was observed for Z -FF incorporated with A at concentrations of 0.2 % and 0.5 %, respectively. This study provides novel insights into the development of Z-based freshness-indicating labels via green electrospinning for intelligent food packaging, especially in livestock and marine products., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2025 The Authors. Published by Elsevier Ltd.)
- Published
- 2025
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42. Anti-inflammatory, anti-colitis, and antioxidant effects of columbianadin against DSS-induced ulcerative colitis in rats via alteration of HO-1/Nrf2 and TLR4-NF-κB signaling pathway.
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Zhang Y, Cao P, Qin D, Zhao Y, Chen X, and Ma P
- Abstract
Background: Ulcerative colitis (UC) is a significant inflammatory bowel disease (IBD) that typically arises from chronic inflammation of the intestinal tract. Report suggest that anti-inflammatory drug plays a crucial role in the protection of UC. The recent study demonstrated that columbianadin has a protective effect against UC induced by dextran sulfate sodium (DSS) in rats through the modulation of HO-1/Nrf2 and TLR4-NF-κB signaling pathways., Material and Methods: In this study, Swiss Wistar rats were utilized, and UC was induced using 2% DSS. The treatment regimen included oral administration of columbianadin (5, 10 and 15 mg/kg) and sulfasalazine to the rats. The body weight, spleen index, disease activity index (DAI), colon length, food and water intake were estimated. Moreover, antioxidant, cytokines, inflammatory and apoptosis parameters were determined. mRNA expression levels were also quantitatively analyzed., Results: Columbianadin treatment significantly (P < 0.001) boosted the body weight and suppressed the DAI. Columbianadin significantly (P < 0.001) enhanced the colon length and repressed the spleen index along with enhanced food and water intake. Columbianadin significantly (P < 0.001) suppressed the level of lactate dehydrogenase (LDH), myeloperoxidase (MPO) and altered the level of oxidative stress parameters such as catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), malonaldehyde (MDA), nitric oxide (NO), SA; cytokines level such as interleukin (IL)-1, 1β, 6, 10, 17, 18, TNF-α; inflammatory parameters viz., cyclooxygenase-2 (COX-2), prostaglandin (PGE
2 ), inducible nitric oxide synthetase (iNOS), nuclear factor kappa B (NF-κB), transforming growth factor (TGF-β); apoptosis parameters include Bax, Bcl-2, Bcl-2/Bax ratio, caspase-1 and A-caspase-3 activity, respectively. Columbianadin significantly altered the mRNA expression of IFN-γ, IL-6, IL-1β, IL-8, TNF-α, NF-κB, TLR4, Bcl-2, caspase-9, Bax, p38, ASC, MCP-1, ZO-1, and Ocln. While this study focused on COX-2 modulation as a marker of inflammatory response, no direct measurements or inferences were made regarding leukotriene activity, which involves a separate lipoxygenase pathway., Conclusion: Columbianadin exhibited the protective effect against DSS-induced UC via alteration of HO-1/Nrf2 and TLR4-NF-κB signaling pathway., Competing Interests: Declarations. Conflict of interest: The authors have not disclosed any competing interests., (© 2025. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)- Published
- 2025
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43. Clinical-grade human dental pulp stem cells improve adult hippocampal neural regeneration and cognitive deficits in Alzheimer's disease.
- Author
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Xiong W, She W, Liu Y, Zhou H, Wang X, Li F, Li R, Wang J, Qin D, Jing S, Duan X, Jiang C, Xu C, He Y, Wang Z, and Ye Q
- Subjects
- Animals, Humans, Mice, Stem Cell Transplantation methods, Nerve Regeneration physiology, Stem Cells physiology, Stem Cells cytology, Cell Differentiation, Cognitive Dysfunction therapy, Male, Neurons physiology, Wnt Signaling Pathway, Dental Pulp cytology, Alzheimer Disease therapy, Alzheimer Disease physiopathology, Hippocampus, Mice, Transgenic, Disease Models, Animal
- Abstract
Background: Disrupted hippocampal functions and progressive neuronal loss represent significant challenges in the treatment of Alzheimer's disease (AD). How to achieve the improvement of pathological progression and effective neural regeneration to ameliorate the intracerebral dysfunctional environment and cognitive impairment is the goal of the current AD therapy. Methods: We examined the therapeutic potential of clinical-grade human derived dental pulp stem cells (hDPSCs) in cognitive function and neuropathology in AD. Specifically, we investigated the effect of neural crest-specific derived hDPSCs on endogenous neural regeneration and long-term efficacy following a single transplantation in the triple-transgenic mouse model (3xTg-AD). Results: Our research demonstrated that a single administration of clinical-grade hDPSCs yielded dramatic short-term therapeutic benefits (5 weeks) and sustained partial efficacy (6 months) with respect to improving cognitive impairment and delaying typical pathological progression in 3xTg-AD mice. Intriguingly, exogenous hDPSCs were robustly self-differentiated into newborn functional neurons in the hippocampus of 3xTg-AD mice. The foremost evidence is provided that hDPSCs promote endogenic neural regeneration by enhancing the activation of the Wnt/β-catenin pathway, which may contribute to stabilizing the hippocampal neural network to reverse memory deficits. Conclusion: These findings highlight the multifunctional potential of hDPSCs in AD treatment, which enhances cognition through alleviating neuropathology and providing neural regenerative driving force. Understanding these multiplicity effects is critical to advancing the clinical translation of stem cell-based therapies for AD., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)
- Published
- 2025
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44. LcMYB5, an R2R3-MYB family gene from Lonicera caerulea L., enhances drought and salt tolerance in transgenic tobacco and blue honeysuckle.
- Author
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Fu C, Bian C, Chen J, Zhang Q, Qin D, Li J, Zhang P, Huo J, and Gang H
- Subjects
- Transcription Factors genetics, Transcription Factors metabolism, Gene Expression Regulation, Plant, Genes, Plant, Lonicera genetics, Lonicera physiology, Lonicera metabolism, Plants, Genetically Modified genetics, Nicotiana genetics, Nicotiana physiology, Salt Tolerance genetics, Plant Proteins genetics, Plant Proteins metabolism, Droughts
- Abstract
MYB transcription factors exert crucial functions in enhancing plant stress tolerance, which is impacted by soil drought and salinity. In our study, the R2R3-type MYB transcription factor gene LcMYB5 from blue honeysuckle (Lonicera caerulea L.) was successfully cloned and identified, and confirmed its nuclear localization. LcMYB5 overexpression was vastly enhanced drought and salt tolerance in both blue honeysuckle and tobacco seedlings. After drought stress, transgenic tobacco exhibited an average survival rate of 70.30%, while most wild-type (WT) plants perished, resulting in a survival rate of only 15.33%. Following salt stress, the average survival rate for transgenic tobacco reached 77.24%, compared to just 22.47% for WT plants. Measurements indicated, that transgenic tobacco had higher proline content than WT, as well as higher superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity. Transgenic tobacco decreased chlorophyll content less dramatically than WT tobacco, despite both tobaccos having decreased chlorophyll content. Furthermore, the level of malondialdehyde (MDA) and relative conductivity were lower in transgenic tobacco compared to WT. Furthermore, LcMYB5 overexpression significantly increased the expression levels of key genes related to drought stress (NCED1, NCED2, PYL4, PYL8, and CBL1) and salt stress (NHX1, SOD, CAT1, SOS1, and HSP17.8), thus improving transgenic tobacco's stress tolerance. Compared to WT blue honeysuckle, transiently transformed LcMYB5-expressing blue honeysuckle exhibited milder damage under stress conditions, a significant increase in chlorophyll and proline content was observed, the activities of SOD, POD and CAT were also significantly increased, the increase in MDA content and relative conductivity is relatively small. Additionally, In addition, transient expression of LcMYB5 can also positively regulate the expression of these five key genes of drought stress and five key genes of salt stress, so as to improve the resistance of transgenic blue honeysuckle to drought and salt stress. In summary, our study reveals the important regulatory role of LcMYB5 in plant resistance to drought and salt stress, providing theoretical support and potential application value for further improving crop stress resistance., Competing Interests: Declaration of competing interest The authors declare no conflict of interest regarding the presented data in the manuscript., (Copyright © 2024 Elsevier GmbH. All rights reserved.)
- Published
- 2025
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45. Polymer-Formulated Nerve Growth Factor Shows Effective Therapeutic Efficacy for Cerebral Microinfarcts.
- Author
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Yan L, Xu K, Liu C, Yu F, Guo J, Hou L, Feng Y, Yang M, Gong Q, Qin D, Qin M, Wang Y, Su H, and Lu Y
- Subjects
- Animals, Mice, Microglia metabolism, Microglia drug effects, Cerebral Infarction drug therapy, Cerebral Infarction pathology, Apoptosis drug effects, Oxidative Stress drug effects, Disease Models, Animal, Neurons drug effects, Neurons metabolism, Neurons pathology, Nerve Growth Factor therapeutic use, Nerve Growth Factor metabolism, Polymers chemistry, Blood-Brain Barrier metabolism
- Abstract
Cerebral microinfarcts represent the most prevalent form of ischemic brain injury in the elderly, particularly among those suffering from dementia, Alzheimer's disease, and vascular risk factors. Despite their commonality, effective treatments have remained elusive. Herein, a novel treatment utilizing a polymer-formulated nerve growth factor capable of crossing the blood-brain barrier is reported, which effectively reduced oxidative stress and neuronal apoptosis, reshaped microglia polarization at infarct sites, and decreased the overall microinfarct burden, leading to notable improvements in behavioral and cognitive functions in a mouse model. This work provides a promising new avenue for the treatment of cerebral microinfarcts and other neurodegenerative diseases., (© 2024 Wiley‐VCH GmbH.)
- Published
- 2025
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46. Combination transcriptomic and metabolomic reveal deterioration of the blue honeysuckle (Lonicera caerulea L.) fruit and candidate genes regulating metabolism in the post-harvest stage.
- Author
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Chen J, Fu C, Wang H, Sun X, Ma K, Yang H, Qin D, Huo J, and Gang H
- Subjects
- Metabolomics methods, Metabolome, Gene Expression Profiling methods, Lonicera genetics, Lonicera metabolism, Fruit genetics, Fruit metabolism, Gene Expression Regulation, Plant, Transcriptome
- Abstract
Blue honeysuckle, a new berry with high nutritional value, possesses typical berry postharvest properties, including extreme perishability, rapid quality loss, and high sensitivity to microbial infections. At present, the underlying mechanisms of postharvest quality deterioration, senescence, and low-temperature regulation remain largely unknown. This study aimed to elucidate the metabolic shifts and genetic regulation underlying the preservation or deterioration of blue honeysuckle during storage at room temperature (25 °C) and low temperature (4 °C). Storage at 4 °C inhibited fruit decay and preserved better visual quality, weight, firmness, and total soluble solid and acid contents. We identified 24 key differentially accumulated metabolites that specifically changed during the qualitative shift at room temperature and were effectively regulated by 4 °C. Commonly associated metabolites, sorbitol, succinic acid, malic acid, naringenin, pinobanksin, and taxifolin, characterize the deterioration of blue honeysuckle. These metabolites were integrated with transcriptomic data for weighted correlation network analysis (WGCNA). Regulatory networks were used for the identification of key genes and transcription factors (TFs) influencing sugar, organic acid, flavonoid, and phenolic acid metabolism during storage. The findings provide insight into metabolic regulation and the improvement of flavor in postharvest blue honeysuckle fruit., Competing Interests: Declaration of competing interest The authors declare no conflict of interest regarding the presented data in the manuscript., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2025
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47. Niraparib perturbs autophagosome-lysosome fusion in pancreatic ductal adenocarcinoma and exhibits anticancer potential against gemcitabine-resistant PDAC.
- Author
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Yao Z, Zhang H, Huang K, Huang G, Xi P, Jiang L, Qin D, Chen F, Li S, and Wei R
- Abstract
While poly (adenosine diphosphate-ribose) polymerase inhibitors (PARPi) have achieved specific clinical benefits in a subset of pancreatic ductal adenocarcinoma (PDAC) patients, the potential role of the PARPi niraparib in PDAC necessitates further exploration. In this study, we demonstrated that Niraparib exhibited a pronounced inhibitory effect on autophagy in PDAC both in vitro and in vivo. Mechanistically, this inhibition was primarily attributed to niraparib's ability to disrupt the fusion process between autophagosomes and lysosomes, while potentially exerting a relatively minor impact on the initial stage of autophagy. The blockade effect observed may be mediated via modulation of the ERK signaling pathway, and this effect can be mitigated by the application of an ERK inhibitor (FR180204). Notably, the combined treatment regimen of niraparib and gemcitabine failed to elicit the anticipated synergistic effects in wild-type PANC-1 cells, instead exhibiting pronounced antagonistic interactions. However, in gemcitabine-resistant PANC-1 cells, the combination of niraparib and gemcitabine exhibited modest additive effects. Furthermore, niraparib demonstrated a heightened cytotoxic potency against gemcitabine-resistant PANC-1 cells compared to wild-type PANC-1 cells, both in vitro and in vivo. Our research established that niraparib inhibits late-stage autophagy in PDAC, potentially representing a valuable salvage therapy for gemcitabine-resistant PDAC. Further clinical studies are justified., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)
- Published
- 2025
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48. Corrigendum to "Copper doped carbon dots modified bacterial cellulose with enhanced antibacterial and immune regulatory functions for accelerating wound healing" [Carbohydrate Polymers 346 (2024) 122656].
- Author
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Liu Y, Zhao Y, Guo S, Qin D, Yan J, Cheng H, Zhou J, Ren J, Sun L, Peng H, Wu X, and Li B
- Published
- 2025
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49. Carbon-Oxygen Radical Assisted Growth of Defect-Free Graphene Films Using Low-Temperature Chemical Vapor Deposition.
- Author
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Liu H, Li Y, Wu Y, Qin D, Qiu X, Wang Z, Zhou Q, Yu S, Li Q, Li H, Li S, Yu C, Hu Y, Wang S, Chen B, Song X, Qiang J, Zhou L, Li Y, Xu N, Liu M, Yin W, Sun X, Sun L, and Liu Z
- Abstract
Low-temperature chemical vapor deposition growth of graphene films is a long-term pursuit in the graphene synthesis field because of the low energy consumption, short heating-cooling process and low wrinkle density of as-obtained films. However, insufficient energy supply at low temperature (below 850 °C) usually leads to the difficulty in carbon source dissociation, graphene growth, and defect healing. Herein, a Carbon-Oxygen (C─O) radical assisted strategy is proposed for low-temperature growth of defect-free, wrinkle-free, and single-crystalline graphene films by using methanol precursor. We provide a deep insight into the growth process fueled by methanol precursor, unveiling the dissociation pathway of methanol and the roles of intermediate C─O radicals in carbon attaching and assembling to graphene lattice without defect formation. This method shows promising prospects in the cost-effective production of high-quality graphene films and provides inspiration for growing other 2D materials., (© 2024 Wiley‐VCH GmbH.)
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- 2025
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50. Frontiers of Neurodegenerative Disease Treatment: Targeting Immune Cells in Brain Border Regions.
- Author
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Satyanarayanan SK, Han Z, Xiao J, Yuan Q, Yung WH, Ke Y, Chang RC, Zhu MH, Su H, Su KP, Qin D, and Lee SMY
- Subjects
- Humans, Animals, Choroid Plexus immunology, Meninges immunology, Immune System immunology, Neurodegenerative Diseases immunology, Neurodegenerative Diseases therapy, Brain immunology, Microglia immunology, Blood-Brain Barrier immunology
- Abstract
Neurodegenerative diseases (NDs) demonstrate a complex interaction with the immune system, challenging the traditional view of the brain as an "immune-privileged" organ. Microglia were once considered the sole guardians of the brain's immune response. However, recent research has revealed the critical role of peripheral immune cells located in key brain regions like the meninges, choroid plexus, and perivascular spaces. These previously overlooked cells are now recognized as contributors to the development and progression of NDs. This newfound understanding opens doors for pioneering therapeutic strategies. By targeting these peripheral immune cells, we may be able to modulate the brain's immune environment, offering an alternative approach to treat NDs and circumvent the challenges posed by the blood-brain barrier. This comprehensive review will scrutinize the latest findings on the complex interactions between these peripheral immune cells and NDs. It will also critically assess the prospects of targeting these cells as a ground-breaking therapeutic avenue for these debilitating disorders., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2025
- Full Text
- View/download PDF
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