Search

The Water Framework Directive (WFD) sets the fundamental structure for assessing the status of water bodies in the European Union. Its implementation is currently entering its fourth six-year cycle assisted by a total of 38 guidance documents. The principal objective is to ensure good status for surface and ground waters. The functioning of the WFD is based on detecting the impact of human pressures on biological, physico-chemical, or hydromorphological parameters, and reducing these causal pressures through a program of measures to achieve good status. Climate change can exert a significant influence on ecological status by directly altering parameters monitored, pressure interactions, or influencing the effectiveness of programs of measures. Aquatic systems respond holistically to climate change with different pressures having additive, synergistic, or antagonistic interactions. The challenge is how to adapt the framework to manage aquatic systems in the context of climate change while maintaining focus on implementing measures to tackle key pressures. This paper examines potential approaches, including reassignment of waterbody type, quantifying the portion of Ecological Quality Ratio (EQR) driven by climate change, and creating an assessment module of climatic pressures and ecological responses. The overall purpose is to stimulate discussion and explore ways to incorporate climate change into the WFD structure., Competing Interests: Declaration of competing interest The authors declare no competing interest., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Monitoring programs at sub-national and national scales lack coordination, harmonization, and systematic review and analysis at continental and global scales, and thus fail to adequately assess and evaluate drivers of biodiversity and ecosystem degradation and loss at large spatial scales. Here we review the state of the art, gaps and challenges in the freshwater assessment programs for both the biological condition (bioassessment) and biodiversity monitoring of freshwater ecosystems using the benthic macroinvertebrate community. To assess the existence of nationally- and regionally- (sub-nationally-) accepted freshwater benthic macroinvertebrate protocols that are put in practice/used in each country, we conducted a survey from November 2022 to May 2023. Responses from 110 respondents based in 67 countries were received. Although the responses varied in their consistency, the responses clearly demonstrated a lack of biodiversity monitoring being done at both national and sub-national levels for lakes, rivers and artificial waterbodies. Programs for bioassessment were more widespread, and in some cases even harmonized among several countries. We identified 20 gaps and challenges, which we classed into five major categories, these being (a) field sampling, (b) sample processing and identification, (c) metrics and indices, (d) assessment, and (e) other gaps and challenges. Above all, we identify the lack of harmonization as one of the most important gaps, hindering efficient collaboration and communication. We identify the IUCN SSC Global Freshwater Macroinvertebrate Sampling Protocols Task Force (GLOSAM) as a means to address the lack of globally-harmonized biodiversity monitoring and biological assessment protocols., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Defining nutrient thresholds that protect and support the ecological integrity of aquatic ecosystems is a fundamental step in maintaining their natural biodiversity and preserving their resilience. With increasing catchment pressures and climate change, it is more important than ever to develop clear methods to establish thresholds for status classification and management of waters. This must often be achieved using complex data and should be robust to interference from additional pressures as well as ameliorating or confounding conditions. We use both artificial and real data to examine challenges in setting nutrient thresholds in unbalanced and skewed data. We found significant advantages to using binary logistic regression over other techniques. However, one of the key challenges is objectively selecting a probability from which to derive the nutrient threshold. For this purpose, the examination of the proportions of matching and mismatching status classifications of nutrients and a biological quality element using a confusion matrix is a key step that should be more widely adopted in threshold selection. We examined a large array of statistical measures of classification accuracy and their performance over combinations of skewness and imbalance in the data. The most appropriate threshold probability is a compromise between maximising overall classification accuracy and reducing mismatches expressed as commission (false positives) without excessive omission (false negatives). An application to a lake type indicated total phosphorus thresholds that would be around 50 μg l -1 lower than the threshold achieved by an 'unguided' approach, indicating that this approach is a very significant development meriting attention from national authorities responsible for water management., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

The characterization of lake hydromorphology is crucial to understand the dynamics of biodiversity. In Europe, it is also a regulatory requirement of the Water Framework Directive. However, according to the literature, few methods include this characterization. The aim of this study is to review the state of the art of the methods currently used or under development in European countries to assess lake hydromorphological status for the implementation of the WFD. Our analysis is based on responses to a questionnaire distributed to national experts on hydromorphology of the 28 countries implementing the WFD. Our results highlighted significant progress in the assessment of hydromorphological features and processes. Water level regime, through the range of water flow or existing water management, and structure of the shore zone through macrophytes and substrate characteristics or measurement of lateral connectivity, are the most frequently assessed features. Stratification, surface/groundwater connection and planform pattern are the lake features most frequently omitted from the methods. However, in most of the countries, the development of methods was still in progress to meet the WFD requirement. Definition of reference condition is a central component of all WFD compliant assessment tools but this is a challenge particularly in the assessment of hydromorphological alteration of reservoirs. Similarly, demonstrating strong links between hydromorphological indicators and biological quality elements remains a challenge with many knowledge gaps still evident. These results highlight the need for rapid collection of new environmental data and the need for conceptual and applied research to make methodological progress in assessing lake hydromorphology and ensuring habitat quality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Nutrient targets based on pressure-response models are essential for defining ambitions and managing eutrophication. However, the scale of biogeographical variation in these pressure-response relationships is poorly understood, which may hinder eutrophication management in regions where lake ecology is less intensively studied. In this study, we derive ecology-based nutrient targets for five major ecoregions of Europe: Northern, Central-Baltic, Alpine, Mediterranean and Eastern Continental. As a first step, we developed regressions between nutrient concentrations and ecological quality ratios (EQR) based on phytoplankton and macrophyte communities. Significant relationships were established for 13 major lake types; in most cases, these relationships were stronger for phosphorus than for nitrogen, and stronger for phytoplankton than for macrophytes. Using these regressions, we estimated the total phosphorus (TP) and total nitrogen (TN) concentrations at which lakes of different types are likely to achieve good ecological status. However, in the very shallow lakes of the Eastern Continental region, relations between nutrient and biological communities were weak or non-significant. This can be attributed to high nutrient concentrations (in the asymptotic zone of phosphorus-phytoplankton models) suggesting other factors (light, grazing) limit primary production. However, we also show that fish stocking is a major pressure on Eastern Continental lakes, negatively affecting ecological status: lakes with low fish stocking show low chlorophyll-a concentrations and good ecological status despite high nutrient levels, while the lakes with high fish stocking show high chlorophyll-a and low ecological status. This study highlights the need to better understand lakes in biogeographic regions that have been, for historical reasons, less studied. This, in turn, helps reveal factors that challenge the dominant paradigms of lake assessment and management., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

Eutrophication caused by an excessive presence of nutrients is affecting large portions of European waters with more than 60% of the surface water bodies failing to achieve the primary ambition of water management in Europe, that of good ecological status (GES) with diffuse emission from agriculture being the second most important pressure affecting surface waters. We developed EU wide and regional nutrient targets that define the boundary concentrations between good and moderate status for river and lake total P (TP) and total N (TN) and assessed the gap between actual nutrient concentrations and these targets and considered strategies of nutrient reductions necessary to achieve GES and deliver ecosystem services. The nutrient targets established for rivers ranged from 0.5-3.5 mg/L TN and 11-105 μg/L TP and for lakes 0.5-1.8 mg/L TN and 10-60 μg/L TP. Based on the EU wide targets, 59% of the TN and 57% of the TP river monitoring sites and 64% of the TN and 61% of the TP lake monitoring sites exceed these value and are thus at less than GES. The PCA and step-wise regression for EU basins clearly showed that the basin nutrient export is predominantly related to agricultural inputs. In addition, the step-wise regression models for TN and TP provided the ability to extrapolate the results and quantify the input reductions necessary for reaching the nutrient targets at the EU level. The results suggest that a dual water management strategy would be beneficial and should focus a) on those less polluted rivers and lakes that can easily attain the GES goal and b) on the more highly polluted systems that will improve the delivery of ecosystem services., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)

One key component of any eutrophication management strategy is establishment of realistic thresholds above which negative impacts become significant and provision of ecosystem services is threatened. This paper introduces a toolkit of statistical approaches with which such thresholds can be set, explaining their rationale and situations under which each is effective. All methods assume a causal relationship between nutrients and biota, but we also recognise that nutrients rarely act in isolation. Many of the simpler methods have limited applicability when other stressors are present. Where relationships between nutrients and biota are strong, regression is recommended. Regression relationships can be extended to include additional stressors or variables responsible for variation between water bodies. However, when the relationship between nutrients and biota is weaker, categorical approaches are recommended. Of these, binomial regression and an approach based on classification mismatch are most effective although both will underestimate threshold concentrations if a second stressor is present. Whilst approaches such as changepoint analysis are not particularly useful for meeting the specific needs of EU legislation, other multivariate approaches (e.g. decision trees) may have a role to play. When other stressors are present quantile regression allows thresholds to be established which set limits above which nutrients are likely to influence the biota, irrespective of other pressures. The statistical methods in the toolkit may be useful as part of a management strategy, but more sophisticated approaches, often generating thresholds appropriate to individual water bodies rather than to broadly defined "types", are likely to be necessary too. The importance of understanding underlying ecological processes as well as correct selection and application of methods is emphasised, along with the need to consider local regulatory and decision-making systems, and the ease with which outcomes can be communicated to non-technical audiences., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Triggered by the adoption of the Water Framework Directive, a variety of fish-based systems were developed throughout Europe to assess the ecological status of lakes. This paper provides a comprehensive overview of all existing systems and summarizes sampling methods, fish community traits (metrics) and the relevant anthropogenic pressures assessed by them. Twenty-one European countries developed fish-based assessment systems. Three countries each developed two distinct systems to approach different ecoregions, either to use different data, or to assess different lake types leading to a total number of 24 systems. The most common approach for the setting of reference conditions, used in seventeen systems, was the utilisation of fish communities in comparably undisturbed natural lakes as reference. Eleven used expert judgment, nine historical data and eight modelled relationships. Fourteen systems combined at least two approaches. The most common fish sampling method was a standardized fishing procedure with multimesh-gillnets. Many countries applied combinations of fishing methods, e.g. non-standard gillnets, fyke nets and electrofishing. Altogether 177 metrics were used for index development and each system combined 2-13 metrics. The most common ones were total standardized catches of number and biomass, relative abundance of Perca fluviatilis, Rutilus rutilus, and Abramis brama, feeding preferences, sensitive species, and non-natives. The pressure-response-relationships for these metrics were supported with both correlations established during system development and scientific publications. However, the metrics and their combinations were highly diverse and no metric was applied universally. Our analysis reveals that most fish-based assessment systems address multiple pressures (eutrophication, hydromorphological alterations, fishery pressure and occurrence of non-natives), whilst few are pressure-specific, tackling only eutrophication or acidification. We argue that the value of fish-based systems for lakes lies in their capacity to capture the effect of many different pressures and their interactions which is lacking for most assessment systems based on other biota., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)