1. Single-molecule RNA Imaging Using Mango II Arrays
- Author
-
Adam D. Cawte, Haruki Iino, Peter J. Unrau, David Rueda, and Medical Research Council
- Subjects
Mangifera ,Fluorogenic RNA Aptamer ,Chemistry ,RNA ,food and beverages ,Folding (DSP implementation) ,Superresolution microscopy ,Aptamers, Nucleotide ,0601 Biochemistry and Cell Biology ,Fluorescence ,Fluorescence intensity ,RNA Aptamers ,Single Molecule Microscopy ,Single-Molecule Microscopy ,Biochemistry ,Live cell imaging ,Spinacia oleracea ,RNA-Mango ,0399 Other Chemical Sciences ,Molecule ,RNA dynamics ,Fluorescent Dyes ,Developmental Biology - Abstract
In recent years, fluorogenic RNA aptamers, such as Spinach, Broccoli, Corn, Mango, Coral, and Pepper have gathered traction as an efficient alternative labeling strategy for background-free imaging of cellular RNAs. However, their application has been somewhat limited by relatively inefficient folding and fluorescent stability. With the recent advent of novel RNA-Mango variants which are improved in both fluorescence intensity and folding stability in tandem arrays, it is now possible to image RNAs with single-molecule sensitivity. Here we discuss the protocol for imaging Mango II tagged RNAs in both fixed and live cells.
- Published
- 2021