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This work aimed to evaluate how the C3-CAM shift could contribute to photoprotection and attenuate losses of CO2 uptake in Pereskia aculeata plants under water stress. Plants were subjected to treatments: well-watered (WW), water deficit (WD), and recovery (Rec) in two experimental conditions (greenhouse and growth chamber). Induction of the C3-CAM shift by drought was assessed by leaf acidification, carbohydrate content, photochemical activity, gas exchange, and stomata density and closure during diurnal and nocturnal periods. Leaf acidity was reduced during the night period in WW-plants and increased in WD-plants, suggesting active accumulation of organic acids associated with CAM induction by drought. This apparent CAM induction in WD-plants was associated to increase carbohydrates and significant reduction of leaf water potential (ΨW). WD-plants showed a positive carbon balance associated with a higher net-CO2 uptake (PN) during night period compared to WW-plants. Moreover, WW-plants presented negative values for PN associated with a negative carbon balance at night. In both conditions (WW and WD) plants showed PN near zero at night. However, a positive carbon balance associated with a slight stomatal aperture at during the day and strong closure during night in the WD-plants, suggests that C3-CAM shift, able to maintain CO2 uptake, presented a better trend toward the CAM-cycling model. Together, this study shows that CO2 uptake conferred by C3-CAM shift under drought contributed to photoprotection and better photosynthetic recovery after rehydration of Pereskia aculeata plants. [ABSTRACT FROM AUTHOR]

With the increasing world population and vegan diet, there has been increasing consumer demand for alternative protein sources. A substitute for animal proteins is the plant protein, for instance, leaves. The Pereskia aculeata, known as ora-pro-nóbis, is undoubtedly a leafy vegetable with great potential due to its relatively high protein content (17 to 28%). This study aimed to produce ora-pro-nóbis protein concentrate (OPNPC) from ora-pro-nóbis leaves flour (OPNF) by isoelectric precipitation at three different pH's (3.5, 4.0, and 4.5). The protein extraction by precipitation in different pHs produced OPNPC with protein content and extraction yield ranging from 52 to 55% and 1–4%, respectively. Given the highest yield, the concentrate obtained at pH 3.5 (OPNPC3.5) was selected for further investigation and comparison to OPNF. The differences in color, techno-functional properties, in vitro protein digestibility (IVPD), and structural properties were evaluated. Most techno-functional properties were statistically higher in OPNPC3.5 than in OPNF. These included its water solubility, oil holding capacity, foam capacity and stability, and emulsifying activity and stability. OPNPC3.5 had a higher IVPD (80%) than flour (77%). Scanning electron microscopy and Fourier transform infrared spectroscopy confirmed distinct compositions of materials, which can explain the difference in techno-functional properties. The findings indicate controlling protein extraction conditions as a useful technique to maximize the yield of protein concentrate obtained from ora-pro-nóbis, which was more nutritious and had better techno-functional properties than flour. This demonstrates its potential as an alternative plant-based protein to design healthy and sustainable food products. [ABSTRACT FROM AUTHOR]

Abstract This study evaluated the effects of ora-pro-nobis (Pereskia aculeate) flour supplementation on the in vivo basal antioxidant system of Drosophila melanogaster, and its action on the neural modulation observed by the enzyme acetylcholinesterase (AChE). The flies will receive a standard diet with flour incorporated at 5, 10 and 20% for 7 days. There was no change in food consumption, body weight, protein thiol levels and negative geotaxis behavior. The flies showed a reduction in the basal production of reactive species at concentrations of 10 and 20%, while there was a reduction in lipid peroxidation and catalase activity at all concentrations, accompanied by an increase in the levels of non-protein thiols. Superoxide dismutase activity was reduced in the 5 and 20% groups, while the reduction of superoxide anion in the 10% group may have contributed to the increase in longevity also in the 10% group. Longevity increased in groups 5 and 10%. The open field test may be related to the reduction in AChE activity in the 5, 10 and 20% groups. In general, the data show that supplementation with ora-pro-nobis flour at the concentrations tested did not cause toxicity and modulated the cholinergic system, demonstrating a therapeutic potential.

Pereskia aculeata is a potential new crop species that has both food and medicinal (antinociceptive activity) properties. However, comprehensive genomic research on P. aculeata is still lacking, particularly concerning its organelle genome. In this study, P. aculeata was studied to sequence the mitochondrial genome (mitogenome) and to ascertain the assembly, informational content, and developmental expression of the mitogenome. The findings revealed that the mitogenome of P. aculeata is circular and measures 515,187 bp in length with a GC content of 44.05%. It contains 52 unique genes, including 33 protein-coding genes, 19 tRNA genes, and three rRNA genes. Additionally, the mitogenome analysis identified 165 SSRs, primarily consisting of tetra-nucleotides, and 421 pairs of dispersed repeats with lengths greater than or equal to 30, which were mainly forward repeats. Based on long reads and PCR experiments, we confirmed that two pairs of long-fragment repetitive elements were highly involved with the mitogenome recombination process. Furthermore, there were 38 homologous fragments detected between the mitogenome and chloroplast genome, and the longest fragment was 3962 bp. This is the first report on the mitogenome in the family Cactaceae. The decoding of the mitogenome of P. aculeata will provide important genetic materials for phylogenetic studies of Cactaceae and promote the utilization of species germplasm resources. [ABSTRACT FROM AUTHOR]

Brazil is one of the centers of diversity of Cactaceae, yet studies addressing both pollination biology and the breeding system in Brazilian cacti are scarce. We herein present a detailed analysis of two native species with economic relevance: Cereus hildmannianus and Pereskia aculeata. The first species produce edible, sweet, spineless fruits and the second species produces leaves with high protein content. Pollination studies were undertaken through fieldwork observations in three localities of Rio Grande do Sul, Brazil, over two flowering seasons, totaling over 130 observation hours. Breeding systems were elucidated utilizing controlled pollinations. Cereus hildmannianus is solely pollinated by nectar-gathering species of Sphingidae hawk moths. In contrast, the flowers of P. aculeata are pollinated by predominantly native Hymenoptera but also by Coleoptera and Diptera, which gather pollen and/or nectar. Both cacti species are pollinator-dependent; neither intact nor emasculated flowers turn into fruit, yet whereas C. hildmannianus is self-incompatible, P. aculeata is fully self-compatible. In sum, C. hildmannianus is more restrictive and specialized regarding its pollination and breeding system, whereas P. aculeata is more generalist. Understanding the pollination needs of these species is a necessary starting point towards their conservation but also for their proper management and eventual domestication. [ABSTRACT FROM AUTHOR]

Pereskia aculeata Mill., known as Ora-Pro-Nobis or Barbados gooseberry, arouse the interest of food and pharmaceutical industries due to its bioactive compounds and mucilage. We conducted a peer-reviewed survey using Web of Science, Scopus, Scielo, Science Direct, and Scifinder platforms, as well as patent bases for new products. We selected articles which highlighted composition of leaves and applications in the development of new products. Mucilage shows great potential in the development of complexes or microparticles to transport active molecules. Reports on anti-inflammatory and anticancer properties of P. aculeata leaves open a research field to obtain pharmaceutical products. Emulsifying properties of mucilage have been explored in food processing. Another potential use is the development of films for functional and/or edible packaging. The polysaccharide chain, the main mucilage constituent, can interact with other biopolymers to be explored in colloidal chemistry for the production of biomaterials in the next years. [ABSTRACT FROM AUTHOR]

Abstract The constant need for innovation in the food industries to meet the demands of consumers and the growing market for gluten-free and high-protein products have driven studies of mixed flour. In this study, 'BRS Platina' banana fruits were processed to obtain Whole Green Banana Flour (WGBF) and Green Banana Pulp Flour (GBPF) as well as leaves of Ora-pro-nóbis Flour (OPNF). Mixed flours were prepared by blending banana flours (WGBF or GBPF) with increasing levels of OPNF (from 5 to 25%). Flours were analyzed for physicochemical characteristics, mineral contents, Water Absorption Index (WAI), Water Solubility Index (WSI), and color. The main component of banana flours was starch, in which GBPF has the highest content. The WGBF had a higher mineral content, and in both flours, the potassium was at a greater level. OPNF showed the highest protein and mineral contents. Independent of the type of banana flour, mixed flours were darker, and had higher WAI and considerable increases in Mg, Fe, and Zn than no mixed ones. The blend of banana flours and Ora-pro-nóbis leaf flour can be a viable combination for preparing a variety of food products, due to its nutritional and technological properties.

Brazil is one of the centers of diversity of Cactaceae, yet studies addressing both pollination biology and the breeding system in Brazilian cacti are scarce. We herein present a detailed analysis of two native species with economic relevance: Cereus hildmannianus and Pereskia aculeata. The first species produce edible, sweet, spineless fruits and the second species produces leaves with high protein content. Pollination studies were undertaken through fieldwork observations in three localities of Rio Grande do Sul, Brazil, over two flowering seasons, totaling over 130 observation hours. Breeding systems were elucidated utilizing controlled pollinations. Cereus hildmannianus is solely pollinated by nectar-gathering species of Sphingidae hawk moths. In contrast, the flowers of P. aculeata are pollinated by predominantly native Hymenoptera but also by Coleoptera and Diptera, which gather pollen and/or nectar. Both cacti species are pollinator-dependent; neither intact nor emasculated flowers turn into fruit, yet whereas C. hildmannianus is self-incompatible, P. aculeata is fully self-compatible. In sum, C. hildmannianus is more restrictive and specialized regarding its pollination and breeding system, whereas P. aculeata is more generalist. Understanding the pollination needs of these species is a necessary starting point towards their conservation but also for their proper management and eventual domestication.

Background: The ora-pro-nóbis ( Pereskia aculeata Mill.) is a plant from Brazilian biodiversity used for food and medicinal purposes. It has ample technological potential, however, it is still underutilized, being classified as a Non-Conventional Food Plant (PANC). Prospective studies in intellectual property banks make it possible to expand perspectives for scientific research, enhancing the generation of new products., Objective: Evaluate the patents of products containing Pereskia aculeata Mill. for the areas of food and health in intellectual property databases., Methods: The study was conducted through structured prospective investigation (collection, processing and analysis) in 4 patent databases: National Institute of Intellectual Property (INPI) - Brazil, United States Patent and Trademark Office, World Trade Organization Intellectual Property (WIPO) and Espacenet., Results: The evaluation showed a reduced number of registered patents. In general, 8 patent applications were examined, of which 7 are directly associated with the species (and its derivatives) and 1 is related to a device specially designed for harvesting leaves/fruits and removing thorns. The focus of the patents was the use of the species in the food, pharmaceutical and biotechnological areas, with emphasis on the use of the leaves in the extraction of mucilage and proteins., Conclusion: This study showed that Pereskia aculeata Mill. is a technologically promising plant, because of its nutritional and medicinal composition, and it is important to encourage innovation and the development of new products with the species., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Ora-pro-nobis (Pereskia aculeata) leaves were selected as raw material for biorefinery application based on compressed fluid extractions with green solvents. The fractionation followed the sequential processes: (1) Supercritical fluid extraction (SFE) with CO 2 , (2) Gas expanded liquid extraction (GXL), using CO 2 :Ethanol, and pressurized liquid extraction (PLE) with ethanol, and (3) Subcritical water extraction (SWE). In vitro analysis of total phenolic and total carotenoid contents and antioxidant activity (DPPH and ORAC) were assessed, stipulating PLE method as the second step of the sequential extractions. Total protein and carbohydrate contents were evaluated from SWE samples. Phenolics profile from PLE and SWE samples was assessed by LC-DAD-ESI-MS/MS, identifying 8 phenolic compounds, with quercetin, isorhamnetin and kaempferol as the main detected flavonoids. SWE at 150 and 185 °C provided the highest protein recovery, as the third step in a sequential process. • Integration of process were efficient to produce several high added-value products. • PLE-EtOH was chosen as the second step of the downstream process in the biorefinery. • SWE samples showed the best antioxidant activity related to Caftaric acid. • SWE at 150 and 185 °C showed the best protein recovery. [ABSTRACT FROM AUTHOR]