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6 results on '"Pedro F. Esteban"'

1. Post-COVID Complications after Pressure Ulcer Surgery in Patients with Spinal Cord Injury Associate with Creatine Kinase Upregulation in Adipose Tissue

Author

Mario Martínez-Torija, Pedro F. Esteban, Francisco Javier Espino-Rodríguez, Beatriz Paniagua-Torija, Eduardo Molina-Holgado, Silvia Ceruelo, Gemma Barroso-Garcia, Alba G. Arandilla, Luis F. Lopez-Almodovar, Angel Arevalo-Martin, Juan Antonio Moreno, Daniel Garcia-Ovejero, Mª Carmen Durán-Ruiz, and Rafael Moreno-Luna

Subjects
COVID-19, SARS-CoV-2, SCI, PU, surgery, adipose tissue, Cytology, QH573-671
Abstract

The risk of complications following surgical procedures is significantly increased in patients with SARS-CoV-2 infection. However, the mechanisms underlying these correlations are not fully known. Spinal cord injury (SCI) patients who underwent reconstructive surgery for pressure ulcers (PUs) before and during the COVID-19 pandemic were included in this study. The patient’s postoperative progression was registered, and the subcutaneous white adipose tissue (s-WAT) surrounding the ulcers was analyzed by proteomic and immunohistochemical assays to identify the molecular/cellular signatures of impaired recovery. Patients with SCI and a COVID-19-positive diagnosis showed worse recovery and severe postoperative complications, requiring reintervention. Several proteins were upregulated in the adipose tissue of these patients. Among them, CKMT2 and CKM stood out, and CKM increased for up to 60 days after the COVID-19 diagnosis. Moreover, CKMT2 and CKM were largely found in MGCs within the s-WAT of COVID patients. Some of these proteins presented post-translational modifications and were targeted by autoantibodies in the serum of COVID patients. Overall, our results indicate that CKMT2, CKM, and the presence of MGCs in the adipose tissue surrounding PUs in post-COVID patients could be predictive biomarkers of postsurgical complications. These results suggest that the inflammatory response in adipose tissue may underlie the defective repair seen after surgery.

Published
2022
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2. Endocannabinoid signaling in oligodendroglia

Author

Eduardo Molina‐Holgado, Pedro F. Esteban, Ángel Arevalo‐Martin, Rafael Moreno‐Luna, Francisco Molina‐Holgado, and Daniel Garcia‐Ovejero

Subjects
Central Nervous System, Oligodendroglia, Cellular and Molecular Neuroscience, Neurology, Cell Differentiation, Myelin Sheath, Axons, Endocannabinoids
Abstract

In the central nervous system, oligodendrocytes synthesize the myelin, a specialized membrane to wrap axons in a discontinuous way allowing a rapid saltatory nerve impulse conduction. Oligodendrocytes express a number of growth factors and neurotransmitters receptors that allow them to sense the environment and interact with neurons and other glial cells. Depending on the cell cycle stage, oligodendrocytes may respond to these signals by regulating their survival, proliferation, migration, and differentiation. Among these signals are the endocannabinoids, lipidic molecules synthesized from phospholipids in the plasma membrane in response to cell activation. Here, we discuss the evidence showing that oligodendrocytes express a full endocannabinoid signaling machinery involved in physiological oligodendrocyte functions that can be therapeutically exploited to promote remyelination in central nervous system pathologies.

Published
2022
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3. Anosmin 1 N-terminal domains modulate prokineticin receptor 2 activation by prokineticin 2

Author

Verónica Murcia-Belmonte, María Tercero-Díaz, Diego Barrasa-Martín, Sandra López de la Vieja, Marina Muñoz-López, Pedro F. Esteban, Junta de Comunidades de Castilla-La Mancha, Universidad de Castilla La Mancha, and Fundación Salud 2000

Subjects
Extracellular Matrix Proteins, Humans, Nerve Tissue Proteins, Cell Biology, Kallmann Syndrome, Receptors, G-Protein-Coupled
Abstract

The X-linked form of Kallmann syndrome (KS), characterized by hypogonadotropic hypogonadism and anosmia, is due to mutations in the ANOS1 gene that encodes for the extracellular matrix (ECM) protein anosmin 1. Prokineticins (PKs) exert their biological functions through the activation of the G protein-coupled receptors (GPCRs) prokineticin receptor 1 and 2 (PKR1, 2), and mutations in the PK2 and PKR2 genes are involved in the pathogenesis of KS. We have previously shown interaction between PKR2 and anosmin 1 in vitro. In the current report we present evidence of the modulation of PK2/PKR2 activity by anosmin 1, since this protein is able to enhance the activation of the ERK1/2 (extracellular signal-regulated kinase 1/2) pathway elicited by PK2 through PKR2. We also show that the N-terminal region of anosmin 1, capable of binding to the PK2-binding domain of PKR2, seems to be responsible for this effect. The whey acidic protein domain (WAP) is necessary for this modulatory activity, although data from GST pull-down (glutathione-S-transferase) and analysis of the N267K mutation in the fibronectin type III domain 1 (FnIII.1) suggest the cysteine-rich (CR) and the FnIII.1 domains could assist the WAP domain both in the binding to PKR2 and in the modulation of the activation of the receptor by PK2. Our data support the idea of a modulatory role of anosmin 1 in the biological effects controlled by the PK2/PKR2 system., This research was supported by grants from the Gobierno de Castilla-La Mancha, Spain (PI2009/29) and Fundación Salud 2000, Spain (Merck-Serono Research Grant 2013) to Pedro F. Esteban.

Published
2022

5. Post-COVID Complications after Pressure Ulcer Surgery in Patients with Spinal Cord Injury Associate with Creatine Kinase Upregulation in Adipose Tissue.

Author

Martínez-Torija, Mario, Esteban, Pedro F., Espino-Rodríguez, Francisco Javier, Paniagua-Torija, Beatriz, Molina-Holgado, Eduardo, Ceruelo, Silvia, Barroso-Garcia, Gemma, Arandilla, Alba G., Lopez-Almodovar, Luis F., Arevalo-Martin, Angel, Moreno, Juan Antonio, Garcia-Ovejero, Daniel, Durán-Ruiz, Mª Carmen, and Moreno-Luna, Rafael

Subjects
SPINAL cord surgery, ADIPOSE tissues, PRESSURE ulcers, SPINAL cord injuries, WHITE adipose tissue, COVID-19
Abstract

The risk of complications following surgical procedures is significantly increased in patients with SARS-CoV-2 infection. However, the mechanisms underlying these correlations are not fully known. Spinal cord injury (SCI) patients who underwent reconstructive surgery for pressure ulcers (PUs) before and during the COVID-19 pandemic were included in this study. The patient's postoperative progression was registered, and the subcutaneous white adipose tissue (s-WAT) surrounding the ulcers was analyzed by proteomic and immunohistochemical assays to identify the molecular/cellular signatures of impaired recovery. Patients with SCI and a COVID-19-positive diagnosis showed worse recovery and severe postoperative complications, requiring reintervention. Several proteins were upregulated in the adipose tissue of these patients. Among them, CKMT2 and CKM stood out, and CKM increased for up to 60 days after the COVID-19 diagnosis. Moreover, CKMT2 and CKM were largely found in MGCs within the s-WAT of COVID patients. Some of these proteins presented post-translational modifications and were targeted by autoantibodies in the serum of COVID patients. Overall, our results indicate that CKMT2, CKM, and the presence of MGCs in the adipose tissue surrounding PUs in post-COVID patients could be predictive biomarkers of postsurgical complications. These results suggest that the inflammatory response in adipose tissue may underlie the defective repair seen after surgery. [ABSTRACT FROM AUTHOR]

Published
2022
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6. Hospital Nacional de Paraplejicos Researchers Release New Data on COVID-19 (Post-COVID Complications after Pressure Ulcer Surgery in Patients with Spinal Cord Injury Associate with Creatine Kinase Upregulation in Adipose Tissue)

Subjects
Banco Santander S.A., Adipose tissues, Banking industry, Creatine kinase, Spinal cord injuries -- Complications and side effects, Bedsores -- Complications and side effects, Banking industry, Business, Health, Health care industry
Abstract

2022 MAY 15 (NewsRx) -- By a News Reporter-Staff News Editor at Medical Letter on the CDC & FDA -- Investigators publish new report on COVID-19. According to news reporting [...]

Published
2022

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