3 results on '"Palomares Reséndiz, Gabriela"'
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2. Presence of Chlamydia abortus in colostrum, milk and vaginal discharge samples of sheep
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Martínez Serrano, María Guadalupe, Díaz Aparicio, Efrén, Palomares Reséndiz, Gabriela, Tórtora Pérez, Jorge, Ramírez Álvarez, Hugo, Ortega Hernández, Nieves, Salinas Lorente, Jesús, Morales Álvarez, José Francisco, Cervantes Morali, Juan Julio César, Martínez Serrano, María Guadalupe, Díaz Aparicio, Efrén, Palomares Reséndiz, Gabriela, Tórtora Pérez, Jorge, Ramírez Álvarez, Hugo, Ortega Hernández, Nieves, Salinas Lorente, Jesús, Morales Álvarez, José Francisco, and Cervantes Morali, Juan Julio César
- Abstract
Background: The main transmission route of Chlamydia abortus is by ingesting the microorganism that has been eliminated in vaginal secretions, placental membranes or abortions that contaminate the environment and, possibly, through milk and colostrum. Elimination through vaginal secretions is well documented. However, there are no reports about isolation and identification of C. abortus in the colostrum or milk of infected sheep, so it is important to determine whether or not C. abortus may be present in these secretions, which are the only food of lambs. Objective: To detect C. abortus in colostrum, milk, and vaginal secretions of sheep with a history of reproductive disorders. Methods: Colostrum, milk, and vaginal exudates were collected from 66 sheep. The samples were inoculated in mouse fibroblast cell cultures and the presence of C. abortus determined by direct immunofluorescence. Results: 19 out of 66 colostrum samples (28.7%), 14 out of 66 milk samples (21.2%) and 17 out of 66 vaginal swabs (25.7%) were positive for C. abortus. The 50 samples positive for isolation and detected by immunofluorescence, together with 42 negative samples were subjected to qPCR to amplify a fragment of the ompA gene from C. abortus. Thirty-eight of the 92 samples processed by this technique were positive for C. abortus. Conclusion: The results demonstrated the presence of C. abortus in a high proportion in colostrum, milk and vaginal secretions of infected sheep. To the best of our knowledge, this is the first field study confirming the presence of C. abortus in colostrum, which shows that excretion of Chlamydia by lactogenesis could occur in the first hours after birth., Antecedentes: A principal via de transmissão da Chlamydia abortus é a ingestão do microrganismo que foi eliminado nas secreções vaginais, membranas placentárias ou abortos que contaminam o meio ambiente e, possivelmente, através do leite e colostro. A eliminação pelas secreções vaginais está bem documentada. No entanto, não há relatos de isolamento e identificação de C. Abortus no colostro ou leite de ovelhas infectadas, por isso é importante verificar se a bactéria pode estar ou não presente nessas secreções, único alimento dos cordeiros. Objetivo: Detectar a presença de C. Abortus no colostro, leite e secreções vaginais de ovelhas com histórico de distúrbios reprodutivos. Métodos: Para isolar e identificar C. Abortus nessas secreções, foram coletados colostro, leite e exsudato vaginal de 66 ovelhas. As amostras foram inoculadas em cultura de células de fibroblastos de camundongo e a presença da bactéria determinada por imunofluorescência direta. Resultados: 19 de 66 amostras de colostro (28,7%), 14 de 66 amostras de leite (21,2%) e 17 de 66 esfregaços vaginais (25,7%) sendo positivos. As 50 amostras positivas para isolamento e detectadas por imunofluorescência, juntamente com as 42 negativas, foram submetidas a qPCR para amplificar um fragmento do gene ompA de C. Abortus. Trinta e oito das 92 amostras processadas por esta técnica foram positivas para C. Abortus. Conclusão: Os resultados do presente estudo demonstraram a presença de C. Abortus em alta proporção no colostro, leite e secreções vaginais de ovelhas infectadas. Este trabalho é o primeiro estudo de campo na literatura científica confirmando a presença de C. Abortus no colostro, o que mostra que a excreção da clamídia por lactogênese pode ocorrer nas primeiras horas após o nascimento., Antecedentes: La principal vía de transmisión de C. abortus es la ingestión del microorganismo que ha sido eliminado en las secreciones vaginales, membranas placentarias, abortos y, posiblemente, a través de la leche y el calostro. La eliminación a través de secreciones vaginales está bien documentada. Sin embargo, no existen reportes del aislamiento e identificación de C. abortus en el calostro o la leche de ovejas infectadas, por lo que es importante determinar si la bacteria puede o no estar presente en estas secreciones, que son el único alimento de los corderos. Objetivo: Detectar la presencia de C. abortus in calostro, leche y secreciones vaginales de ovejas con antecedentes de problemas reproductivos. Método: Con el propósito de aislar e identificar C. abortus en estas secreciones, se recolectó calostro, leche y exudado vaginal de 66 ovejas. Las muestras fueron inoculadas en cultivos celulares de fibroblastos de ratón y se determinó la presencia de la bacteria por inmunofluorescencia directa. Resultados: Fueron positivas 19 de 66 muestras de calostro (28,7%), 14 de 66 muestras de leche (21,2%) y 17 de 66 hisopos vaginales (25,7%). Las 50 muestras positivas al aislamiento y detectadas por inmunofluorescencia, junto con 42 negativas se sometieron a qPCR para amplificar un fragmento del gen ompA de C. abortus; 38 de las 92 muestras procesadas por esta técnica fueron positivas para C. abortus. Conclusión: Los resultados del presente estudio demostraron la presencia de C. abortus en una alta proporción en el calostro, la leche y las secreciones vaginales de ovejas infectadas. Este es el primer estudio de campo que confirma la presencia de C. abortus en calostro, lo que demuestra que la excreción de clamidia por lactogénesis podría ocurrir en las primeras horas después del nacimiento.
- Published
- 2022
3. Important infectious diseases in goat production in Mexico: history, challenges and outlook.
- Author
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Palomares Reséndiz, Gabriela, Aguilar Romero, Francisco, Flores Pérez, Carlos, Gómez Núñez, Luis, Gutiérrez Hernández, José, Herrera López, Enrique, Limón González, Magdalena, Morales Álvarez, Francisco, Pastor López, Francisco, and Díaz Aparicio, Efrén
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MEXICAN history , *COMMUNICABLE diseases , *Q fever , *GOAT diseases , *DIAGNOSIS , *MILK yield , *BRUCELLOSIS , *ZOONOSES - Abstract
Goat production in Mexico is concentrated in poorer areas in arid and semi-arid areas of the country's north and center, particularly in the Mixtee region of Oaxaca, Guerrero and Puebla. Because goats can survive in near desert conditions their production of milk and animals is a valuable nutritional contribution to human diets in these areas. Disease in goats in Mexico has generally received scant attention, however researchers at the IN1FAP have studied this species and its pathologies. This review focuses on the main diseases affecting goats in Mexico and the research in this area by the INIFAP. In most of the goat herds studied in Mexico reproductive disorders are often caused by diseases such as brucellosis, leptospirosis and chlamydia, all considered to be endemic and potentially zoonotic. Respiratory and gastrointestinal disorders are the main ailments in kids. High frequencies of arthritic encephalitis, a disease caused by infection with small ruminant lentiviruses, and paratuberculosis and caseous lymphadenitis, both bacterial in origin, have been documented in productive-age goats throughout Mexico. All three are chronic, causing producers to mistakenly assume they have no major impact on productivity. Q fever, a known zoonotic, is currently considered exotic in Mexico, but in other countries is frequently associated with reproductive disorders, abortions and occasional respiratory problems in goats. The INIFAP has addressed all the above diseases. It was instrumental in diagnosing and controlling brucellosis, the principal bacterial zoonosis in Mexico. Researchers at INIFAP have also helped to determine that diseases previously considered exotic in Mexico have become endemic, and then developing the tools needed for their diagnosis. The INIFAP has made vital contributions to understanding the national disease panorama in goats and transferring diagnostic and treatment technologies to livestock laboratories nationwide. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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