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Salinity is a major abiotic stressor that can significantly impact plant growth and productivity. To evaluate the physiological responses of sweet pepper (Capsicum annuum L. Ps301) to gradual salinity stress (SLS) and shock salinity stress (STS), an experiment was conducted using a factorially based completely randomized design (CRD). The sodium chloride (NaCl) induced salinity treatments were administered in the nutrient solution in two forms: STS and SLS. The SLS was applied for 8 days, with stress levels increasing every 2 days; STS was administered for 48 h. The signaling response was activated more rapidly and abruptly in response to STS; however, after the various adaptive responses to salinity, these signals were more activated in response to SLS. The STS led to plant growth a greater decrease than SLS. The electrolyte leakage (EL) increased under both SLS and STS by 60% and 61% compared to the control, respectively. Other stress indices, such as phenol and abscisic acid (ABA) content, increased by 26% and 71% under SLS compared to STS. Salinity increased the leaf water potential (LWP) in two salt regimes, and the root water potential (RWP) in STS conditions only. Salinity stress decreased plasma membrane intrinsic protein gene expression in roots, especially in STS compared to SLS. The STS increased plasma membrane intrinsic protein (PIP) expression in leaves more than SLS. It was concluded that salinity impairs the growth and physiological characteristics of pepper; however, the signaling and stress indices respond differently to STS and SLS application. [ABSTRACT FROM AUTHOR]

Fugitive emissions from petrochemical facilities have become a major concern due to their impact on plant productivity, the environment, and health. In regard to health, petrochemical workers are at higher occupational health (OH) risk due to their continuous exposure to these harmful emissions. Inherent OH and safety indexes are the most common methods used for assessing OH risk due to fugitive emissions. These methods usually focus on the sources of health hazards, such as chemical substances, process conditions, and process equipment. Therefore, these methods are considered good for measuring the severity of the OH risk. However, based on the source, pathway, receptor (SPR) model, the OH risk due to fugitive emissions is also dependent on the pathway and receptor, where leak and exposure hazards may take place, respectively. For a holistic OH risk assessment, these hazards need to be considered. This was achieved by developing an OH risk assessment methodology that provides an effective assessment that takes into consideration hazards at the source, pathway, and receptor. This paper focuses on the source part of the SPR model, while the pathway and receptor parts will be covered in future publications. This paper presents an index‐based method named the inherent health hazard level index (IHHLI) developed for evaluating the severity of the fugitive emission‐induced OH risk. The IHHLI is developed by an expert‐based selection of the most common and relevant health hazard indicators published in the literature. Based on industry testing, the IHHLI can provide a reliable OH hazard evaluation. [ABSTRACT FROM AUTHOR]

The growth of plants hinges on a complex interplay of biochemical and physiological activities across various growth stages. These intricate processes dynamically adapt to different environmental conditions, shaping both plant development and productivity. This study explores the impact of greenhouse climate on the growth, yield, and biochemistry of winter-grown cherry tomatoes 'Cheramy F1'. A randomized complete block design (RCBD) under split plot arrangements (3 Rows) with three replications (3 plants from each row) was adopted. The data were collected on various dates during the period extending from December to March of two consecutive growing seasons in 2022 and 2023, and presented as averages. An analysis of variance was applied to statistically analyze the collected data at a confidence level of p < 0.05. The climatic conditions in the greenhouse were calculated as temperature ranging from a minimum of 10.5 °C to the maximum of 41.3 °C by an average of 21.2 °C during the vegetative stage and from 8.2 °C to 32.3 °C by an average of 20.9 °C during the fruit-bearing stage, with an average CO2 concentration fluctuated within the range of 385.61 ppm to 510.30 ppm and an average light intensity of 94.62 to 240.45 W/m². This study assessed various growth parameters such as plant height, leaf growth, stem diameter, leaf spacing, leaf count, leaf area, and inflorescence count per plant, and suggested the optimum range of greenhouse conditions for each stage. The key results of this study revealed the Progressive Growth Report (PGR), predicting daily potential growth rates of plants: plant height, 2.86 to 3.81 cm/day; growth rate of mature older leaf: 0.003988 m2/day; middle younger leaf: 0.008733 m2/day; top nascent leaf: 0.010722 m2/day; three to five leaves per week; and one inflorescence per week. In our accidental observation, we noticed unusual plant growth and yield responses because of the various growing postures and positions that the plants adopted in the greenhouse. An exceedingly significant difference among the inflorescences was found in view of their growth, productivity and biochemical composition. A non-significant interaction was found between the fruit keeping quality (shelf days), fruit height, fruit diameter, and inflorescence number. The present study results highlight the possible responses of greenhouse-grown cherry tomatoes to different ranges of temperature, light intensity, and CO2 concentrations, offering valuable insights for optimizing greenhouse cherry tomatoes cultivation. [ABSTRACT FROM AUTHOR]

Summary Roots are important in agricultural and natural systems for determining plant productivity and soil carbon inputs. Sometimes, the amount of roots in a sample is too much to fit into a single scanned image, so the sample is divided among several scans, and there is no standard method to aggregate the data. Here, we describe and validate two methods for standardizing measurements across multiple scans: image concatenation and statistical aggregation. We developed a Python script that identifies which images belong to the same sample and returns a single, larger concatenated image. These concatenated images and the original images were processed with RhizoVision Explorer, a free and open‐source software. An R script was developed, which identifies rows of data belonging to the same sample and applies correct statistical methods to return a single data row for each sample. These two methods were compared using example images from switchgrass, poplar, and various tree and ericaceous shrub species from a northern peatland and the Arctic. Most root measurements were nearly identical between the two methods except median diameter, which cannot be accurately computed by statistical aggregation. We believe the availability of these methods will be useful to the root biology community. [ABSTRACT FROM AUTHOR]

Premise: Reduced snow cover and increasing temperature variability can increase freezing stress for herbaceous plants in northern temperate regions. Legumes have emerged as a plant functional group that is highly sensitive to these changes relative to other herbaceous species in these regions. We explored root‐nodulating bacterial associations and cyanogenesis as potential mechanisms explaining this relatively low freezing tolerance of legumes. Methods: To examine the influence of bacterial associations, we grew four legume species with or without crushed‐nodule inoculum at three severities of freezing, and three concentrations of nitrogen to disambiguate the direct benefits of increased nitrogen from the total bacterial effect. We quantified cyanogenesis via hydrogen cyanide production in both true leaves and cotyledons for nine legume species. Results: Root nodulation generally only affected legume survival under low nitrogen, when freezing severity was moderate or low. However, for the frost‐surviving plants, the growth advantage provided by nodulation decreased (it was often no longer significant with increasing freezing severity), and greater freezing severity reduced total nodule mass. In contrast, cyanogenesis was only detected in two of the nine species. Conclusions: The diminished performance of nodulated plants in response to freezing could place legumes at a competitive disadvantage and potentially explain their high sensitivity to freezing relative to other herbaceous species in northern temperate regions. Overall, this result has important implications for changes in soil fertility, community composition, and plant productivity in these ecosystems in the context of a changing winter climate. [ABSTRACT FROM AUTHOR]

We conducted a 4-year temperature manipulation experiment in a Mongolian grassland to examine the effect of daytime and nighttime warming on grassland recovery after grazing exclusion. After constructing a livestock exclusion fence in the grassland, we established daytime and daytime-and-nighttime warming treatments within the fenced area by a combination of open-top chambers (OTC) and electric heaters. We measured the numbers of plants and aboveground biomass by species after recording percentage vegetation cover every summer for three warming treatments inside the fence—non-warming, daytime warming, and daytime-and-nighttime warming—and for the grassland outside of the fence. OTCs increased daytime temperature by about 2.0 °C, and heaters increased nighttime temperature by 0.9 °C during the growing period. Grazing exclusion had little effect on grassland biomass but reduced the abundance of poorly palatable species and modified plant community composition. Daytime warming decreased soil moisture and lowered aboveground biomass within the fenced grassland but had little effect on plant community composition. Nighttime warming lowered soil moisture further but its effects on grassland biomass and community composition were undetectable. We concluded that recovery of plant biomass in grasslands degraded by grazing would be lowered by future climate warming through soil drying. Because warming had little effect on the recovery of community composition, adverse effects of warming on grassland recovery might be offset by improving plant productivity through mitigation of soil drying by watering. Soil drying due to nighttime warming might have detectable effects on vegetation when warming persists for a long time. [ABSTRACT FROM AUTHOR]

Two common challenges reported by cannabis growers are low yields and small profit margins. Although recent research of cannabis yield has focused on lighting and nutrition, little research has examined how changes in other cultivation practices may be beneficial. The objective of this study was to evaluate the following two techniques to potentially improve yield: fertilizer restriction (FR) to reduce plant size and, thus, increase plant density and shoot number manipulation (SNM) to reduce shoot length and improve biomass partitioning. The FR technique involves leaching the substrate and providing only tap water for 0, 1, or 2 weeks at the start of flower initiation, whereas SNM involves pinching shoot tips 2, 3, or 4 times to produce 4, 8, or 16 shoots/plant, respectively. This study used a full factorial treatment design for a total of nine treatments (three FR × three SNM). Plants were flowered under 12-hour photoperiods for 8 weeks and then destructively harvested for data collection. The results demonstrated that both techniques improve plant productivity in different ways. The FR technique reduced all mass measurements (g/plant) and decreased plant area (m² /plant); therefore, the yield metrics (kg·m-2 ) increased with the increasing FR treatments. The SNM technique did not affect plant area, but more pinching events resulted in a decrease in reproductive shoot length (cm/shoot) and an increase in inflorescence to trim the dry mass ratio (inflorescence:trim). Shorter shoot lengths are desirable for eliminating trellis support netting, which helps growers reduce material costs and improve labor efficiency during harvest. Increasing the inflorescence:trim may also reduce labor costs related to trimming, which comprise the largest cost of production by many growers. Although both techniques offer advantages, there are trade-offs that must be considered in the context of overall profitability [ABSTRACT FROM AUTHOR]

ABSTRACT Adaptation to abiotic stress is critical for the survival of perennial tree species. Salinity affects plant growth and productivity by interfering with major biosynthetic processes. Detrimental effects of salinity may vary between different plant tissues and cell types. However, spatial molecular mechanisms controlling plant responses to salinity stress are not yet thoroughly understood in perennial trees. We used laser capture microdissection in clones of Populus tremula x alba to isolate palisade and vascular cells of intermediary leaf from plants exposed to 150 mM NaCl for 10 days, followed by a recovery period. Cell‐specific changes in proteins and metabolites were determined. Salinity induced a vascular‐specific accumulation of proteins associated with photorespiration, and the accumulation of serine, 3‐phosphoglycerate and NH4+ suggesting changes in N metabolism. Accumulation of the GLUTAMINE SYNTHETASE 2 protein, and increased GS1.1 gene expression, indicated that NH4+ produced in photorespiration was assimilated to glutamine, the main amino acid translocated in Populus trees. Further analysis of total soluble proteins in stems and roots showed the accumulation of bark storage proteins induced by the salinity treatments. Collectively, our results suggest that the salt‐induced photorespiration in vascular cells mediates N‐reallocation in Populus, an essential process for the adaptation of trees to adverse conditions. [ABSTRACT FROM AUTHOR]

SUMMARY: Drought is one of the most important abiotic stresses, and seriously threatens plant development and productivity. Increasing evidence indicates that chromatin remodelers are pivotal for plant drought response. However, molecular mechanisms of chromatin remodelers‐mediated plant drought responses remain obscure. In this study, we found a novel interactor of BRM called BRM‐associated protein involved in drought response (BAPID), which interacted with SWI/SNF chromatin remodeler BRM and drought‐induced transcription factor Di19. Our findings demonstrated that BAPID acted as a positive drought regulator since drought tolerance was increased in BAPID‐overexpressing plants, but decreased in BAPID‐deficient plants, and physically bound to PR1, PR2, and PR5 promoters to mediate expression of PR genes to defend against dehydration stress. Genetic approaches demonstrated that BRM acted epistatically to BAPID and Di19 in drought response in Arabidopsis. Furthermore, the BAPID protein‐inhibited interaction between BRM and Di19, and suppressed the inhibition of BRM on the Di19‐PR module by mediating the H3K27me3 deposition at PR loci, thus changing nucleosome accessibility of Di19 and activating transcription of PR genes in response to drought. Our results shed light on the molecular mechanism whereby the BAPID‐BRM‐Di19‐PRs pathway mediates plant drought responses. We provide data improving our understanding of chromatin remodeler‐mediated plant drought regulation network. Significance Statement: Drought is an enormous threat to plant growth and reproduction, but the roles of chromatin remodelers in mediating plant drought responses remain indistinct so far. In this study, we illuminated the molecular mechanism of the BAPID‐BRM‐Di19‐PRs pathway in drought response in Arabidopsis, promoting our cognition on chromatin remodeler‐mediated plant drought regulation network. [ABSTRACT FROM AUTHOR]

Changes in rainfall patterns are important environmental factors affecting plant growth, especially when larger precipitation events and prolonged drought periods occur in subtropical regions. There are many studies on how drought reduces plant biomass through drought-sensitive functional traits, but how excess water affects plant growth and ecophysiology is still poorly understood. Therefore, a greenhouse experiment was conducted on Schima superba (Theaceae), a dominant tree species in subtropical forests and commonly used in forestry, in a closed chamber under control (25% soil water content (SWC) as in local forests), drought stress (D, 15% SWC) and moisture stress (W, 35% SWC). Plant growth and ecophysiological traits related to morphology, leaf gas exchange, water potential and structural traits were measured. Compared to control, S. suberba under dry conditions significantly decreased its aboveground biomass, photosynthetic rate (A), leaf water potential and nitrogen use efficiency, but increased intrinsic water use efficiency, root to shoot ratio and specific root length. S. superba under wet conditions also significantly decreased its total biomass, aboveground biomass and specific root length, while W had no effect on A and leaf water potential. Our results indicate that S. superba shows a decrease in carbon gain under drought stress, but less response under wet conditions. This emphasizes the need to consider the strength and frequency of rainfall pattern changes in future studies because rainfall may either alleviate or intensify the effects of drought stress depending on the moisture level, thus suitable water conditions is important for better management of this tree species in subtropical China. [ABSTRACT FROM AUTHOR]

Plants are subjected to various biotic and abiotic stresses that significantly impact their growth and productivity. To achieve balanced crop growth and yield, including for leafy vegetables, the continuous application of micronutrient is crucial. This study investigates the effects of different concentrations of copper sulphate (0, 75, 125, and 175 ppm) on the morphological and biochemical features of Spinacia oleracea and Avena sativa. Morphological parameters such as plant height, leaf area, root length, and fresh and dry weights were optimized at a concentration of 75 ppm copper sulfate. At this concentration, chlorophyll a & b levels increased significantly in Spinacia oleracea (462.9 and 249.8 휇푔/푔), and Avena sativa (404.7 and 437.63휇푔/푔). However, carotenoid content and sugar levels in Spinacia oleracea were negatively affected, while sugar content in Avena sativa increased at 125 ppm (941.6 µg/ml). Protein content increased in Spinacia oleracea (75 ppm, 180.3 µg/ml) but decreased in Avena sativa. Phenol content peaked in both plants at 75 ppm (362.2 and 244.5 µg/ml). Higher concentrations (175 ppm) of copper sulfate reduced plant productivity and health. Plants exposed to control and optimal concentrations (75 and 125 ppm) of copper sulpate exhibited the best health and growth compared to those subjected to higher concentrations. Maximum plant height, leaf area, root length, fresh and dry weights were observed at lower concentrations (75 and 125 ppm) of copper sulfate, while higher concentrations caused toxicity. Optimal copper sulfate levels enhanced chlorophyll a, chlorophyll b, total chlorophyll, protein, and phenol contents but inhibited sugar and carotenoid contents in both Spinacia oleracea and Avena sativa. Overall, increased copper sulfate treatment adversely affected the growth parameters and biochemical profiles of these plants. [ABSTRACT FROM AUTHOR]

Plant phenotypic plasticity plays an important role in nitrogen (N) acquisition and use under nitrogen-limited conditions. However, this role has never been quantified as a function of N availability, leaving it unclear whether plastic responses should be considered as potential targets for selection. A combined modelling and experimentation approach was adopted to quantify the role of plasticity in N uptake and plant yield. Based on a greenhouse experiment we considered plasticity in two maize (Zea mays) traits: root-to-leaf biomass allocation ratio and emergence rate of axial roots. In a simulation experiment we individually enabled or disabled both plastic responses for maize stands grown across six N levels. Both plastic responses contributed to maintaining a higher N uptake, and plant productivity as N availability declined compared with stands in which plastic responses were disabled. We conclude that plastic responses quantified in this study may be a potential target trait in breeding programs for greater N uptake across N levels while it may only be important for the internal use of N under N-limited conditions in maize. Given the complexity of breeding for plastic responses, an a priori model analysis is useful to identify which plastic traits to target for enhanced plant performance. [ABSTRACT FROM AUTHOR]

Summary Observational evidence indicates that tree leaf area may acclimate in response to changes in water availability to alleviate hydraulic stress. However, the underlying mechanisms driving leaf area changes and consequences of different leaf area allocation strategies remain unknown. Here, we use a trait‐based hydraulically enabled tree model with two endmember leaf area allocation strategies, aimed at either maximizing carbon gain or moderating hydraulic stress. We examined the impacts of these strategies on future plant stress and productivity. Allocating leaf area to maximize carbon gain increased productivity with high CO2, but systematically increased hydraulic stress. Following an allocation strategy to avoid increased future hydraulic stress missed out on 26% of the potential future net primary productivity in some geographies. Both endmember leaf area allocation strategies resulted in leaf area decreases under future climate scenarios, contrary to Earth system model (ESM) predictions. Leaf area acclimation to avoid increased hydraulic stress (and potentially the risk of accelerated mortality) was possible, but led to reduced carbon gain. Accounting for plant hydraulic effects on canopy acclimation in ESMs could limit or reverse current projections of future increases in leaf area, with consequences for the carbon and water cycles, and surface energy budgets. [ABSTRACT FROM AUTHOR]

Water processes secure plant survival and maintain their ecosystem function. Salinity affectswater processes, but themechanisms remain unclear andmay depend on the degree of salinity stress. To improve the understanding of the cooperation of plant organs involved in water processes under salinity stress, we determined hydraulic, gas exchange, and physiological and biochemical parameters in Populus euphratica Oliv. under different salinity stresses. The results suggested that P. euphratica enhanced water transport efficiency in a salinity-stress environment, and the strengthening effect of roots in the water transfer process was greater than that of the aboveground parts. P. euphratica also increased water use efficiency and water transport efficiency inmild andmoderate salinity stress (less than 200mmol/L NaCl) but was adversely affected by heavy salinity stress (more than 300 mmol/L NaCl). Furthermore, P. euphratica increased its water storage by regulating antioxidant enzyme scavenging capacity and osmoregulation, which resulted in coordinated greater water utilization and enhanced water transport among plant organs and indicated that the adverse effects on water processes triggered by salinity stress depended on the extent of salt stress. P. euphratica lessened stress-induced damage and maintained plant productivity by coordination and cooperation of water processes under certain levels of salinity. Research on the coordination and cooperation involving water processes in riparian forests in saline areas provides the scientific basis for riparian plant protection and restoration. [ABSTRACT FROM AUTHOR]

Summary: Plants naturally harbor diverse microbiomes that can dramatically impact their health and productivity. However, it remains unclear how fungal microbiome diversity, especially in the phyllosphere, impacts intermicrobial interactions and consequent nonadditive effects on plant productivity.Combining manipulative experiments, field collections, culturing, microbiome sequencing, and synthetic consortia, we experimentally tested for the first time how foliar fungal community diversity impacts plant productivity. We inoculated morning glories (Ipomoea hederifolia L.) with 32 phyllosphere consortia of either low or high diversity or with single fungal taxa, and measured effects on plant productivity and allocation.We found the following: (1) nonadditive effects were pervasive with 56% of fungal consortia interacting synergistically or antagonistically to impact plant productivity, including some consortia capable of generating acute synergism (e.g. > 1000% increase in productivity above the additive expectation), (2) interactions among 'commensal' fungi were responsible for this nonadditivity in diverse consortia, (3) synergistic interactions were approximately four times stronger than antagonistic effects, (4) fungal diversity affected the magnitude but not frequency or direction of nonadditivity, and (5) diversity affected plant performance nonlinearly with the highest performance in low‐diversity treatments.These findings highlight the importance of interpreting plant–microbiome interactions under a framework that incorporates intermicrobial interactions and nonadditive outcomes to understand natural complexity. See also the Commentary on this article by Whitaker, 243: 2050–2051. [ABSTRACT FROM AUTHOR]

Biotic and abiotic environmental cues are major factors influencing plant growth and productivity. Interactions with biotic (e.g. symbionts and pathogens) and abiotic (e.g. changes in temperature, water, or nutrient availability) factors trigger signaling and downstream transcriptome adjustments in plants. While bulk RNA-sequencing technologies have traditionally been used to profile these transcriptional changes, tissue homogenization may mask heterogeneity of responses resulting from the cellular complexity of organs. Thus, whether different cell types respond equally to environmental fluctuations, or whether subsets of the responses are cell-type specific, are long-lasting questions in plant biology. The recent breakthrough of single-cell transcriptomics in plant research offers an unprecedented view of cellular responses under changing environmental conditions. In this review, we discuss the contribution of single-cell transcriptomics to the understanding of cell-type-specific plant responses to biotic and abiotic environmental interactions. Besides major biological findings, we present some technical challenges coupled to single-cell studies of plant–environment interactions, proposing possible solutions and exciting paths for future research. [ABSTRACT FROM AUTHOR]

Introduction: Despite their adverse environmental effects, modern agriculture relies heavily on agrochemicals to manage diseases and pests and enhance plant growth and productivity. Some of these functions could instead be fulfilled by endophytes from the plant microbiota, which have diverse activities beneficial for plant growth and health. Methods: We therefore used a microbiome-guided top-down approach to select ten bacterial strains from different taxa in the core microbiome of tomato plants in the production chain for evaluation as potential bioinoculants. High-quality genomes for each strain were obtained using Oxford Nanopore long-read and Illumina short-read sequencing, enabling the dissection of their genetic makeup to identify phyto-beneficial traits. Results: Bacterial strains included both taxa commonly used as biofertilizers and biocontrol agents (i.e. Pseudomonas and Bacillus) as well as the less studied genera Leclercia, Chryseobacterium, Glutamicibacter, and Paenarthorbacter. When inoculated in the tomato rhizosphere, these strains promoted plant growth and reduced the severity of Fusarium Crown and Root Rot and Bacterial Spot infections. Genome analysis yielded a comprehensive inventory of genes from each strain related to processes including colonization, biofertilization, phytohormones, and plant signaling. Traits directly relevant to fertilization including phosphate solubilization and acquisition of nitrogen and iron were also identified. Moreover, the strains carried several functional genes putatively involved in abiotic stress alleviation and biotic stress management, traits that indirectly foster plant health and growth. Discussion: This study employs a top-down approach to identify new plant growth-promoting rhizobacteria (PGPRs), offering an alternative to the conventional bottom-up strategy. This method goes beyond the traditional screening of the strains and thus can expand the range of potential bioinoculants available for market application, paving the way to the use of new still underexplored genera. [ABSTRACT FROM AUTHOR]

Global warming is a leading environmental stress that reduces plant productivity worldwide. Several beneficial microorganisms reduce stress; however, the mechanism by which plant–microbe interactions occur and reduce stress remains to be fully elucidated. The aim of the present study was to elucidate the mutualistic interaction between the plant growth-promoting rhizobacterial strain SH-19 and soybeans of the Pungsannamul variety. The results showed that SH-19 possessed several plant growth-promoting traits, such as the production of indole-3-acetic acid, siderophore, and exopolysaccharide, and had the capacity for phosphate solubilisation. The heat tolerance assay showed that SH-19 could withstand temperatures up to 45 °C. The strain SH-19 was identified as P. megaterium using the 16S ribosomal DNA gene sequence technique. Inoculation of soybeans with SH-19 improved seedling characteristics under high-temperature stress. This may be due to an increase in the endogenous salicylic acid level and a decrease in the abscisic acid level compared with the negative control group. The strain of SH-19 increased the activity of the endogenous antioxidant defense system, resulting in the upregulation of GSH (44.8%), SOD (23.1%), APX (11%), and CAT (52.6%). Furthermore, this study involved the transcription factors GmHSP, GmbZIP1, and GmNCED3. The findings showed upregulation of the two transcription factors GmbZIP1 (17%), GmNCED3 (15%) involved in ABA biosynthesis and induced stomatal regulation, similarly, a downregulation of the expression pattern of GmHSP by 25% was observed. Overall, the results of this study indicate that the strain SH-19 promotes plant growth, reduces high-temperature stress, and improves physiological parameters by regulating endogenous phytohormones, the antioxidant defense system, and genetic expression. The isolated strain (SH-19) could be commercialized as a biofertilizer. [ABSTRACT FROM AUTHOR]

Αbstract: Microbial inocula are considered a promising and effective alternative solution to the use of chemical fertilizers to support plant growth and productivity since they play a key role in the availability and uptake of nutrients. Here, the effect of a beneficial of a fungal root endophyte, Fusarium solani strain K (FsK), on nutrient acquisition efficiency of the legume Lotus japonicus was studied, and putative mode‐of‐action of the endophyte at a molecular level was determined. Plant colonization with the endophyte resulted in increased shoot and root fresh weight under Fe deficiency compared to control nutrient conditions. Plant inoculation with FsK was associated with a significant increase in macro‐ and micronutrient concentration in leaves at an early stage of endophyte inoculation and a replenishment of Fe content under prolonged iron starvation. The mechanistic basis of the plant growth promotion capabilities of the endophyte is exerted at the transcriptional level since we recorded changes in the expression levels of genes related to iron uptake in FsK‐colonized plants under stress conditions compared to uninoculated plants. In addition, the observed changes in the ethylene biosynthesis‐related genes suggest a possible implication of ethylene in the mode of action used by FsK to enhance plant response to nutrient stress conditions. Finally, we demonstrated that the endophyte possesses a reductive high‐affinity Fe uptake system and identified a ferric reductase that was induced in planta under Fe deficiency conditions, indicating that this fungal Fe homeostasis mechanism may result in a benefit in nutrient acquisition for the plant as well. [ABSTRACT FROM AUTHOR]

In this study, we explore the interplay between the plant hormones gibberellins (GA), brassinosteroids (BR), and Indole‐3‐Acetic Acid (IAA) in their collective impact on plant shade avoidance elongation under varying light conditions. We focus particularly on low Red:Far‐red (R:FR) light conditions achieved by supplementing the background light with FR. We characterized the tomato internode response to low R:FR and, with RNA‐seq analysis, we were able to identify some of the potential regulatory hormonal pathways. Through a series of exogenous pharmacological modulations of GA, IAA, and BR, we demonstrate that GA and BR are sufficient but also necessary for inducing stem elongation under low R:FR light conditions. Intriguingly, while IAA alone shows limited effects, its combination with GA yields significant elongation, suggesting a nuanced hormonal balance. Furthermore, we unveil the complex interplay of these hormones under light with low R:FR, where the suppression of one hormone's effect can be compensated by the others. This study provides insights into the hormonal mechanisms governing plant adaptation to light, highlighting the intricate and adaptable nature of plant growth responses. Our findings have far‐reaching implications for agricultural practices, offering potential strategies for optimizing plant growth and productivity in various lighting environments. [ABSTRACT FROM AUTHOR]

Measuring ecosystem multifunctionality (EMF) could be a valuable method for assessing whether nature‐based solutions, such as introducing non‐native species to benefit ecosystem functioning, yield net positive outcomes. While EMF metrics may provide a coarse overview of the functioning of a system, such metrics can obscure the interactions among multiple simultaneously occurring ecosystem functions. This could limit the predictive value and overall efficacy of EMF metrics for the management of environmental change impacts on ecosystem functioning.We carried out a mesocosm experiment with dung beetle temporal species richness (diversity) treatments in a pasture ecosystem in Aotearoa New Zealand and quantified cascading biotic and abiotic effects of introduced dung beetles (Onthophagus binodis and Onitis alexis) on multiple ecosystem functions. We measured nine individual ecosystem functions affected by dung beetles, including dung removal, total soil carbon, nitrogen, phosphorus, soil microbial respiration, nitrate N, ammonium N, anaerobically mineralisable N and pasture biomass production.We first tested the effects of temporal dung beetle diversity on the nine individual ecosystem functions. We then tested how introduced dung beetle temporal diversity affects ecosystem multifunctionality by calculating EMF metrics using the averaging, threshold and the Hill numbers approaches. Finally, to investigate the underlying interactions among individual functions, we tested the direct and indirect effects of introduced dung beetles on the nine ecosystem functions using structural equation modelling (SEM).Only one out of the nine individual ecosystem functions was significantly affected by dung beetle introductions, whereas five of the six calculated EMF metrics increased significantly with temporal diversity of introduced dung beetles. However, when modelled within an SEM framework, dung beetle temporal diversity was found to directly affect dung removal, but with cascading effects on total N and anaerobically mineralisable N.Our results suggest that introducing and increasing dung beetle temporal diversity enhances the provisioning of multiple ecosystem functions, but that our ability to detect and explain changes in ecosystem multifunctionality is critically dependent on how EMF is quantified. Read the free Plain Language Summary for this article on the Journal blog. [ABSTRACT FROM AUTHOR]

The cocoa plantation in Suruh Village, Trenggalek Regency is the main supplier of cocoa beans to UPH Rumah Coklat owned by the Department of Agriculture and Food of Trenggalek Regency. For example, in cocoa farmers' participation in the "Tunggal Jaya" Farmers Group, farmers are taught to make rorak and worm palaces to deal with climate change which helps maintain soil moisture and increase fertility. The objectives of this study are to determine 1) the level of farmer activeness, 2) the level of productivity, 3) the level of income, and 4) the correlation between farmer activeness in farmer groups with productivity and income of cocoa farming in Suruh Village, Trenggalek Regency. The number of samples used was 111 respondents. Data analysis used the Likert scale, productivity analysis, income analysis, and Pearson correlation. The results of the study showed: 1) cocoa farmer activeness in farmer groups in Suruh Village, Trenggalek Regency is in the active category with an overall average score of 3.56; 2) the average productivity of cocoa farming is in the medium category at 0.54 tons/ha/year; 3) the average income of cocoa farming is Rp6,016,562/year; 4) the correlation between farmer activeness with productivity is 0.865, farmer activeness with income is 0.760, and productivity with income is 0.868. The direction of the three correlations is positive and significant. [ABSTRACT FROM AUTHOR]

Common beans are a widely cultivated crop in East and Central Africa. This study aimed to assess the factors affecting local bean production and marketing at the household level. Using a non-experimental research design, the study surveyed 111 farmers from the Isangati division in Mbeya District. Crosssectional data were collected through expert sampling methods, and regression analysis was employed to examine the influence of socio-economic factors on the production and marketing of local bean varieties. The results indicated that variables such as age, education, occupation, and farm size significantly influenced bean production (p<0.01), as did market accessibility and the acceptability of bean varieties (p<0.01). The study concluded that proximity to markets, socio-economic conditions, and consumer preferences play a crucial role in shaping bean production and marketing outcomes. As a result, interventions should be tailored to meet both user and consumer preferences. [ABSTRACT FROM AUTHOR]

Background/Objectives: Nitrogen is an essential macroelement for plant growth and productivity. Calcium (Ca2+) acts as a critical second messenger in numerous adaptations and developmental processes in plants. The Calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) signaling pathway has been demonstrated to be involved in multiple intracellular ion homeostasis of plants in response to stresses. However, whether CIPKs are involved in nitrate deficiency stress remains largely unknown. Methods: In this study, we screened Arabidopsis thaliana T-DNA insertion mutants of the CIPK family under nitrate deficiency conditions by a reverse genetic strategy. Results: We found that the cipk1 mutant showed a shorter primary root and had a lower fresh weight and total N content compared with wildtype (WT) plants under nitrate deficiency. The CIPK1 complementation lines completely rescued the sensitive phenotype. Additionally, CIPK1 mutation caused nitrogen-starvation marker genes to be decreased under nitrate deficiency. We further found that CIPK1 interacted with teosintebranched 1/cycloidea/proliferating cell factor 1-20 (TCP20) in a yeast two-hybrid system. Conclusions: Collectively, our results reveal a novel role of CIPK1 in response to nitrate deficiency in Arabidopsis. [ABSTRACT FROM AUTHOR]

The salinity and alkalinity of soils are two fundamental factors that limit plant growth and productivity. For that reason, a field study conducted at Sakha Agric. Res. Station in Egypt during the 2022–2023 winter season aimed to assess the impact of gypsum (G), compost (C), and zinc foliar application in two images, traditional (Z1 as ZnSO4) and nanoform (Z2 as N-ZnO), on alleviating the saline–sodic conditions of the soil and its impact on wheat productivity. The results showed that the combination of gypsum, compost, and N-ZnO foliar spray (G + C + Z2) decreased the soil electrical conductivity (EC), sodium adsorption ratio (SAR), and exchangeable sodium percentage (ESP) by 14.81%, 40.60%, and 35.10%, respectively. Additionally, compared to the control, the G + C + Z2 treatment showed improved nutrient content and uptake as well as superior wheat biomass parameters, such as the highest grain yield (7.07 Mg ha−1), plant height (98.0 cm), 1000-grain weight (57.03 g), and straw yield (9.93 Mg ha−1). Interestingly, foliar application of N-ZnO was more effective than ZnSO4 in promoting wheat productivity. Principal component analysis highlighted a negative correlation between increased grain yield and the soil EC and SAR, whereas the soil organic matter (OM), infiltration rate (IR), and plant nutrient content were found to be positively correlated. Furthermore, employing the k-nearest neighbors technique, it was predicted that the wheat grain yield would rise to 7.25 t ha−1 under certain soil parameters, such as EC (5.54 dS m−1), ESP (10.02%), OM (1.41%), bulk density (1.30 g cm−3), infiltration rate (1.15 cm h−1), and SAR (7.80%). These results demonstrate how adding compost and gypsum to foliar N-ZnO can improve the soil quality, increase the wheat yield, and improve the nutrient uptake, all of which can support sustainable agriculture. [ABSTRACT FROM AUTHOR]

Cold stress adversely affects rice growth, particularly at the early vegetative growth stage. In higher plants, nitrogen metabolism plays a central role in amino acid metabolism, plant defense mechanisms and productivity. This report investigated the effects of cold stress and supplemental γ-aminobutyric acid (GABA) under cold stress on nitrogen metabolism in rice seedlings. Cold stress resulted in a greater increase in the transformation to NH4+ by nitrate reductase (NR) in roots, it further resulted in lower levels of NO3 - content in roots, weakened glutamine glutamate (GOGAT/GS) pathway and elevated glutamate dehydrogenase (GDH) pathway of rice seedlings. Whereas, compared with cold stress, supplementation of GABA (2.5 mmol·L-1) could increase relative water content (79.43%) and biomass (34.15%) of rice seedlings. GABA could act as an amplifier of stress signal conduction/transduction to increase NR activity and promote NO3 - assimilation in leaves. In addition, GABA elicited the Ca2+ signaling pathway which could promote the GDH pathway and GABA shunt, increase the activities of GS and GDH, and the expression of OsGAD2 and OsGDH family. The GABA might increase the ratio of the Glu family and avoid NH4+ toxicity in order to raise the concentration of organic compounds and alleviate the harmful consequences of cold stress. Based on these observations, this study proposed that GABA mediated cold tolerance in rice seedlings by activating Ca2+ burst and subsequent crosstalk among Ca2+ signaling, GDH pathway and GABA shunt. [ABSTRACT FROM AUTHOR]

Climate variability significantly impacts plant growth, making it crucial to monitor ecosystem performance for optimal carbon sequestration, especially in the context of rising atmospheric CO2 levels. Net Primary Productivity (NPP), which measures the net carbon flux between the atmosphere and plants, serves as a key indicator. This study uses the CASA (Carnegie–Ames–Stanford Approach) model, a radiation use efficiency method, to assess the spatio-temporal dynamics of NPP in Togo from 1987 to 2022 and its climatic drivers. The average annual NPP over 36 years is 4565.31 Kg C ha−1, with notable extremes in 2017 (6312.26 Kg C ha−1) and 1996 (3394.29 Kg C ha−1). Productivity in natural formations increased between 2000 and 2022. While climate change and land use negatively affect Total Production (PT) from 2000 to 2022, they individually enhance NPP variation (58.28% and 188.63%, respectively). NPP shows a strong positive correlation with light use efficiency (r2 = 0.75) and a moderate one with actual evapotranspiration (r2 = 0.43). Precipitation and potential evapotranspiration have weaker correlations (r2 = 0.20; 0.10), and temperature shows almost none (r2 = 0.05). These findings contribute to understanding ecosystem performance, supporting Togo's climate commitments. [ABSTRACT FROM AUTHOR]

Sugarcane plays a pivotal role in the Brazilian economy as a primary crop. This semi-perennial crop allows for multiple harvests throughout its life cycle. Given its longevity, farmers need to be mindful of avoiding gaps in sugarcane fields, as these interruptions in planting lines negatively impact overall crop productivity over the years. Recognizing and mapping planting failures becomes essential for replanting operations and productivity estimation. Due to the scale of sugarcane cultivation, manual identification and mapping prove impractical. Consequently, solutions utilizing drone imagery and computer vision have been developed to cover extensive areas, showing satisfactory effectiveness in identifying gaps. However, recognizing small gaps poses significant challenges, often rendering them unidentifiable. This study addresses this issue by identifying and mapping gaps of any size while allowing users to determine the gap size. Preliminary tests using YOLOv5 and ImageJ 1.53k demonstrated a high success rate, with a 96.1% accuracy in identifying gaps of 50 cm or larger. These results are favorable, especially when compared to previously published works. [ABSTRACT FROM AUTHOR]

The effect of mulching and nutrition on the growth and yield of sweet basil was examined by experimenting with four mulch treatments, viz., without mulch, black plastic, silver plastic, and organic mulch combined with four different nutrient compositions, viz., a 100% recommended dose of fertilizer (RDF), a combination of 75% RDF with humic acid, 75% RDF with microbial consortia and 75% RDF, and humic acid and microbial consortia, which served as sixteen treatment combinations with 100% RDF without mulch as a control. Among the different treatments, mulch had a significant effect on growth and yield parameters, with black plastic mulch having the maximum plant height (88.20 cm), number of branches (14.95), plant spread (5415 cm2), stem diameter (12.64 mm), fresh herbage yield (24.55 t/ha) and dry herbage yield (8.72 t/ha). Thus, the study revealed that among the various mulch treatments, black plastic mulch significantly enhanced the growth and yield parameters of sweet basil, demonstrating its efficacy in promoting optimal plant development and productivity. [ABSTRACT FROM AUTHOR]

Salinity stress is the most pernicious abiotic stress negatively affecting crop productivity around the globe. Soil salinity is significantly decreasing the agricultural lands every year which is posing serious food security threats. The global population is rapidly increasing and it demands the use of every soil for crop production. Salt stress negatively affects plant growth and development by inducing ionic, osmotic, and oxidative stress and it also negatively affects soil biological properties and microbial activities. Using microbial-based techniques is considered an effective, and environmentally friendly way to mitigate stress effects and improve crop production. The application of plant growth-promoting rhizobacteria (PGPR) is an imperious way to improve plant productivity and reclaim salt-affected soils. The use of PGPR can reduce the production of reactive oxygen species (ROS), facilitate nutrient uptake, and regulate gene expression, osmolyte, and hormone synthesis which helps mitigate deleterious impacts of salinity. Besides this, PGPR also improves antioxidant activities, and they produce organic acids, and siderophores which help to counter ROS production. Thus, in the present review, we discussed the different mechanisms of PGPR in modulating plant growth under saline conditions. We also identified different research gaps that must be abridged in future studies. This review will provide better insights into the present knowledge and will help to improve crop production in saline soils using PGRP. [ABSTRACT FROM AUTHOR]

The optimal timing of transition from vegetative to floral reproductive phase is critical for plant productivity and agricultural yields. Light plays a decisive role in regulating this transition. The B‐box (BBX) family of transcription factors regulates several light‐mediated developmental processes in plants, including flowering. Here, we identify a previously uncharacterized group II BBX family member, BBX13/COL15, as a negative regulator of flowering under long‐day conditions. BBX13 is primarily expressed in the leaf vasculature, buds, and flowers, showing a similar spatial expression pattern to the major flowering time regulators CO and FT. bbx13 mutants flower early, while BBX13‐overexpressors exhibit delayed flowering under long days. Genetic analyses showed that BBX13 acts upstream to CO and FT and negatively regulates their expression. BBX13 physically interacts with CO and inhibits the CO‐mediated transcriptional activation of FT. In addition, BBX13 directly binds to the CORE2 motif on the FT promoter, where CO also binds. Chromatin immunoprecipitation data indicates that BBX13 reduces the in vivo binding of CO on the FT promoter. Through luciferase assay, we found that BBX13 inhibits the CO‐mediated transcriptional activation of FT. Together, these findings suggest that BBX13/COL15 represses flowering in Arabidopsis by attenuating the binding of CO on the FT promoter. [ABSTRACT FROM AUTHOR]

Diverse and complex microbiomes are found in virtually every environment on Earth. Bacteria and fungi often co-dominate environmental microbiomes, and there is growing recognition that bacterial-fungal interactions (BFI) have significant impacts on the functioning of their associated microbiomes, environments, and hosts. Investigating BFI in vitro remains a challenge, particularly when attempting to examine interactions at multiple scales of system complexity. Fabricated devices can provide control over both biotic composition and abiotic factors within an experiment to enable the characterization of diverse BFI phenotypes such as modulation of growth rate, production of biomolecules, and alterations to physical movements. Engineered devices ranging from microfluidic chips to simulated rhizosphere systems have been and will continue to be invaluable to BFI research, and it is anticipated that such devices will continue to be developed for diverse applications in the field. This will allow researchers to address specific questions regarding the nature of BFI and how they impact larger microbiome and environmental processes such as biogeochemical cycles, plant productivity, and overall ecosystem resilience. Devices that are currently used for experimental investigations of bacteria, fungi, and BFI are discussed herein along with some of the associated challenges and several recommendations for future device design and applications. [ABSTRACT FROM AUTHOR]

Enhancing crop photosynthesis through genetic engineering technologies offers numerous opportunities to increase plant productivity. Key approaches include optimizing light utilization, increasing cytochrome b6f complex levels, and improving carbon fixation. Modifications to Rubisco and the photosynthetic electron transport chain are central to these strategies. Introducing alternative photorespiratory pathways and enhancing carbonic anhydrase activity can further increase the internal CO2 concentration, thereby improving photosynthetic efficiency. The efficient translocation of photosynthetically produced sugars, which are managed by sucrose transporters, is also critical for plant growth. Additionally, incorporating genes from C4 plants, such as phosphoenolpyruvate carboxylase and NADP-malic enzymes, enhances the CO2 concentration around Rubisco, reducing photorespiration. Targeting microRNAs and transcription factors is vital for increasing photosynthesis and plant productivity, especially under stress conditions. This review highlights potential biological targets, the genetic modifications of which are aimed at improving photosynthesis and increasing plant productivity, thereby determining key areas for future research and development. [ABSTRACT FROM AUTHOR]

The use of burned plant biomass ashes could help not only with respect to utilizing combustion residues, but also with respect to optimizing the nutrition of cultivated agricultural plants without harming the environment. With this aim, a pot experiment of the effects of multi-crop biomass ash on faba bean seedlings was carried out in the Academy of Agriculture of the Vytautas Magnus University (VMU). Four ash fertilization rates were tested: 1. unfertilized (N0, comparative-control treatment); 2. fertilized at a low rate (N1, 200 kg ha−1); 3. fertilized at an average rate (N2, 1000 kg ha−1); 4. fertilized at a high rate (N3, 2000 kg ha−1). Final observations showed that ash fertilization significantly increases the height of faba bean sprouts by 21–38%, the length of the roots by 10–20% and the chlorophyll concentration in the leaves by 17%. The average green biomass of faba bean sprouts consistently increased with increasing fertilization rate, from 56% to 209%. Dried biomass increased by 160–220%. With increasing ash fertilization rate, the percentage of dry matter in the roots decreased by 10–50%. We recommend fertilizing faba bean with medium (1000 kg ha−1) and high (2000 kg ha−1) ash rates, as these rates led to the largest plants with the highest productivity potential. [ABSTRACT FROM AUTHOR]

Satureja rechingeri Jamzad (known as "Jatra" in Persian), which belongs to the Lamiaceae family, is a rich source of essential oil particularly carvacrol, and rosmarinic acid. Drought stress has a detrimental impact on the physiological and biochemical parameters of plants, leading to a decline in plant productivity. Melatonin (MT), a new plant growth regulator found abundantly in plants, has been found to enhance the plant's internal resistance to various environmental stresses. The present study aimed to examine the impact of exogenously applied MT on the agro-morphological, physio-biochemical, and phytochemical traits of S. rechingeri plants cultivated under different levels of drought stress. The results indicated that plants treated with 200 µM MT obtained the highest plant height, length and width of leaf, fresh, dry and drug weight under different drought stress levels. The highest values of relative water content (RWC) (93.5%) and chlorophyll content (15.4 mg/g FW) were recorded by MT 200 µM and 100 µM, respectively, in 100% FC. Drought stress treatments (40, 60, and 80% FC) without foliar spray of MT significantly enhanced the H2O2 content, electrolyte leakage, and malondialdehyde content in leaves, whereas MT treatment under drought stress significantly decreased the above parameters. The lowest H2O2 content (11.5 nmol/g), electrolyte leakage (3.08%), and malondialdehyde content (0.78 µM/g) were obtained by 200 µM MT at 100% FC. In contrast, drought stress treatment increased the total phenol content (TPC), rosmarinic acid (RA), essential oils (EOs) content and yield, and carvacrol. The maximum values of TPC (28.1 mg GAE/g DW), EOs content (3.63%) and yield (0.96%), and carvacrol (95.66%) were achieved by 200 µM MT at 40% FC. The highest RA content (7.43 mg GAE/g DW) was recorded in 100 µM MT at 40% FC. Thus, foliar spray MT has the potential to enhance plant growth through the mitigation of reactive oxygen species (ROS)-induced oxidative harm, as well as the augmentation of photosynthesis pigments, secondary metabolites such as phenolics, EOs levels, overall antioxidant scavenging capacity, and the preservation of RWC during periods of drought stress. [ABSTRACT FROM AUTHOR]

The phenotyping of plant roots is essential for improving plant productivity and adaptation. However, traditional techniques for assembling root phenotyping information are limited and often labor-intensive, especially for woody plants. In this study, an advanced approach called accurate and detailed quantitative structure model-based (AdQSM-based) root phenotypic measurement (ARPM) was developed to automatically extract phenotypes from Ginkgo tree root systems. The approach involves three-dimensional (3D) reconstruction of the point cloud obtained from terrestrial laser scanning (TLS) to extract key phenotypic parameters, including root diameter (RD), length, surface area, and volume. To evaluate the proposed method, two approaches [minimum spanning tree (MST)-based and triangulated irregular network (TIN)-based] were used to reconstruct the Ginkgo root systems from point clouds, and the number of lateral roots along with RD were extracted and compared with traditional methods. The results indicated that the RD extracted directly from point clouds [coefficient of determination (R2) = 0.99, root-mean-square error (RMSE) = 0.41 cm] outperformed the results of 3D models (MST-based: R2 = 0.71, RMSE = 2.20 cm; TIN-based: R2 = 0.54, RMSE = 2.80 cm). Additionally, the MST-based model (F1 = 0.81) outperformed the TIN-based model (F1 = 0.80) in detecting the number of first-order and second-order lateral roots. Each phenotyping trait fluctuated with a different cloud parameter (CP), and the CP value of 0.002 (r = 0.94, p < 0.01) was found to be advantageous for better extraction of structural phenotypes. This study has helped with the extraction and quantitative analysis of root phenotypes and enhanced our understanding of the relationship between architectural parameters and corresponding physiological functions of tree roots. [ABSTRACT FROM AUTHOR]

The article evaluated the genetics of six cucumbers (Cucumis sativus L.) cultivars and 30 hybrids based on morpho-yield traits. The dominant and epistatic gene effects managed the fruit weight in three cucumber hybrids (C-25/1, A-6, and A-9), while additive gene effects in three other hybrids were supreme. For productivity per plant, dominant genes with epistatic effects controlled these in three accessions, while additive effects played a special role in manifesting the trait in three other hybrid (C-25/1, A-6, and A-9) genes. Likewise, dominant genes with epistatic effects controlled the yield per plant trait in three accessions, while additive effects influenced the attribute in three other hybrids (C-25/1, A-6, and A-9). According to these traits, the promising hybrids were С-25/1 х С25/2, С-26 х С-25/2, С-26 х С25/1, А-9 х С-26, and А-6 х С-25/1. The cucumber F1 hybrids C-26 x C29, A-9 x A-6, and A-9 x C-25/2 showed characteristics of large fruits weighing 108.2 to 113.1 g. In the accessions, C-25/1, A-6, and A-9, the additive gene effects dominated the studied trait (σ2ĝi > σ2si). The superior-in-yield hybrid combinations were C-26 x C-29, A-9 x A-6, and A-6 x A-9, with a total fruit yield of 691 to 769 g per plant. [ABSTRACT FROM AUTHOR]

Soybean (Glycine max L.) is one of the most essential sources of high-grade protein and oil. Although growing soybeans can occur in different environmental conditions and are very productive in warm temperatures, their cultivation in cold climates, including Northern Kazakhstan, leads to considerable yield losses. The presented study sought to investigate soybean cultivars growing in the cold climate of northern Kazakhstan and find the optimal ratio of the growing season duration and crop yields. Soybean productivity and the cropping season length revealed a significant negative correlation. Early flowering is one of the relevant approaches for reducing the growing season and improving soybean seed production. A MADS-box SEPALLATA3 (GmSEP3) gene expression, known to promote regular and early flowering, received analysis. The GmSEP3a expression in soybean cultivars with high yield was also valid, with a significant positive correlation between GmSEP3a expression and soybean yield. The soybean cultivars, viz., Suiyang 1, Sibiryachka, No. 86, Niva, and Beidou 43 surfaced as high-yielding cultivars under environmental conditions of Northern Kazakhstan. The pertinent study revealed that the GmSEP3a gene can become a gene marker for screening early-ripening soybean cultivars with high yields in low-temperate climates. The promising results can also help identify potential soybean cultivars for cold-weather cultivation. [ABSTRACT FROM AUTHOR]

Root system architecture (RSA) encompasses a range of physical root attributes, including the lateral roots (LRs), root hairs and adventitious roots, in addition to the primary or main root. This overall structure is a crucial trait for efficient water and mineral capture alongside providing anchorage to the plant in the soil and is vital for plant productivity and fitness. RSA dynamics are dependent upon various environmental cues such as light, soil pH, water, mineral nutrition and the belowground microbiome. Among these factors, light signaling through HY5 significantly influences the flexibility of RSA by controlling different signaling pathways that converge at photoreceptors-mediated signaling, also present in the 'hidden half'. Furthermore, several phytohormones also drive the formation and emergence of LRs and are critical to harmonize intra and extracellular stimuli in this regard. This review endeavors to elucidate the impact of these interactions on RSA, with particular emphasis on LR development and to enhance our understanding of the fundamental mechanisms governing the light-regulation of LR growth and physiology. [ABSTRACT FROM AUTHOR]

Spatial information about plant health and productivity are essential when assessing the progress towards Sustainable Development Goals such as life on land and zero hunger. Plant health and productivity are strongly linked to a plant's phenological progress. Remote sensing, and since the launch of Sentinel-1 (S1), specifically, radar-based frameworks have been studied for the purpose of monitoring phenological development. This study produces insights into how crop phenology shapes S1 signatures of PolSAR features and InSAR coherence of wheat, canola, sugar beet. and potato across multiple years and orbits. Hereby, differently smoothed time series and a base line of growing degree days are stacked to estimate the patterns of occurrence of extreme values and break points. These patterns are then linked to in situ observations of phenological developments. The comparison of patterns across multiple orbits and years reveals that a single optimized fit hampers the tracking capacities of an entire season monitoring framework, as does the sole reliance on extreme values. VV and VH backscatter intensities outperform all other features, but certain combinations of phenological stage and crop type are better covered by a complementary set of PolSAR features and coherence. With regard to PolSAR features, alpha and entropy can be replaced by the cross-polarization ratio for tracking certain stages. Moreover, a range of moderate incidence angles is better suited for monitoring crop phenology. Also, wheat and canola are favored by a late afternoon overpass. In sum, this study provides insights into phenological developments at the landscape level that can be of further use when investigating spatial and temporal variations within the landscape. [ABSTRACT FROM AUTHOR]

Fusarium graminearum, a devastating fungal pathogen, causes great economic losses to crop yields worldwide. The present study investigated the potential of Streptomyces pratensis S10 to alleviate F. graminearum stress in wheat seedlings based on plant growth-promoting and resistance-inducing assays. The bioassays revealed that S10 exhibited multiple plant growth-promoting properties, including the production of siderophores, 1-aminocyclopropane-1-carboxylic acid deaminase (ACC), and indole-3-acetic acid (IAA), phosphate solubilization, and nitrogen fixation. Meanwhile, the pot experiment demonstrated that S10 improved wheat plant development, substantially enhancing wheat height, weight, root activity, and chlorophyll content. Consistently, genome mining identified abundant genes associated with plant growth promotion. S10 induced resistance against F. graminearum in wheat seedlings. The disease incidence and disease index reduced by nearly 52% and 65% in S10 pretreated wheat seedlings, respectively, compared with those infected with F. graminearum only in the non-contact inoculation assay. Moreover, S10 enhanced callose deposition and reactive oxygen species (ROS) accumulation and induced the activities of CAT, SOD, POD, PAL, and PPO. Furthermore, the quantitative real-time PCR (qRT-PCR) results indicated that S10 pretreatment increased the expression of SA- (PR1.1, PR2, PR5, and PAL1) and JA/ET-related genes (PR3, PR4a, PR9, and PDF1.2) in wheat seedlings upon F. graminearum infection. In summary, S. pratensis S10 could be an integrated biological agent and biofertilizer in wheat seedling blight management and plant productivity enhancement. [ABSTRACT FROM AUTHOR]

Background and aim: Soil salinity presents major constraint for symbiotic root-microbe interactions and overall crop productivity. While compost amendments are effective, availability is limited in drylands where salinity is prevalent. As an alternative approach, we investigated the foliar application of signaling compounds. Methods: In this study, cowpea plants were evaluated under the treatments of foliar application of strigolactones (SL), salicylic acid (SA), SL and SA or SLSA, and coumarins (CMR), under soil amendment with gypsum (GP) and compost (CMP), and unamended natural saline soil as the control soil (CS) treatment. The microbiome structure in the rhizosphere, roots, leaves, and seeds were assessed, along with root traits, nodulation, arbuscular mycorrhizal (AMF) colonization, nutrient concentrations, and biomass yield. Results: Several treatments exhibited positive effects, with CMP and SLSA yielding the most comprehensive improvements. The SLSA outperformed CMP in enhancing nodulation and AMF colonization. Only CMP induced a significant shift in the rhizosphere and root microbiome structure, while the leaf and seed microbiomes remained stable. An AMF Rhizophagus in the rhizosphere and AMF Diversispora in the root endosphere of SLSA treatment, and Actinobacteria and Streptomyces in the root endosphere of the SLSA treatment showed increased abundance. The increased abundance of several microbial groups with SLSA indicated their effectiveness as beneficial keystone taxa, which appears to be an influential factor for impacting salinity tolerance in the legume. Conclusions: Overall, these findings confirm that the application of signaling compounds is a viable strategy for enhancing symbiotic root-microbe interactions and improving plant productivity in saline soils. [ABSTRACT FROM AUTHOR]

Background and aims: Afforestation can alter belowground microbial diversity and affect soil‒plant feedback, which is crucial for understanding nutrient cycles and ecosystem productivity. Most studies have focused on the carbon and nitrogen cycles in forest ecosystems; however, there are few studies on the effect of afforestation in soil microbial diversity on phosphorus (P) nutrients. In this study, we explored the effect of soil microbial communities on soil‒plant P feedback under long-term afforestation. Methods: Typical poplar plantations were selected from the Lhasa River Basin. We assessed the P content, activity of extracellular enzyme related to P acquisition, rhizosphere soil microbes, and mechanism of microbial community. Results: Increased bacterial richness and evenness were beneficial to soil and plant P nutrition; however, the community differentiation of bacteria and fungi had no positive effect on the P content. Ecological stochasticity drove for the assembly of the rhizosphere soil microbial community after afforestation on the Tibetan Plateau. Homogenizing dispersal dominated bacterial and fungal assemblies. Ecological drift was another stochastic process that affected the microbial assembly. Conclusion: Bacterial richness and evenness had positive effects on soil‒plant P feedback, whereas microbial differentiation did not. Homogenizing dispersal and ecological drift are intrinsic factors that regulate the effects of microbial community diversity on soil‒plant P feedback processes. This study provides the evidence on the intrinsic mechanism of belowground microbial diversity influencing soil‒plant P feedback. It also provides new insights into the selection of plantation species on the Tibetan Plateau from the perspective of enhancement of P nutrition by microbes. [ABSTRACT FROM AUTHOR]

Drought is one of the most adverse factors affecting plant growth and productivity. Identifying elite genotypes and their ideotypic traits conferring high yield potential and drought tolerance is critical in selecting and breeding drought‐tolerant wheat cultivars. In this study, we conducted field experiments at the Xinxiang Agricultural Comprehensive Experimental station in the North China Plain from 2018 to 2020 and assessed 209 wheat cultivars released since the 1940s under irrigated and nonirrigated conditions. Then, we selected drought‐tolerant cultivars by classifying them into four groups based on yield response to drought stress and several drought indices. Finally, the key ideotypic traits associated with high yield potential and drought tolerance were identified. Results indicated that the grain yield of the 209 cultivars decreased on average by 10.4% and 9.4% under nonirrigated treatment in 2018–2019 and 2019–2020, respectively, relative to full irrigation. The high‐yielding cultivars under both irrigation treatments are characterised by a compact plant type, larger thousand‐grain weight, larger chlorophyll content, higher leaf photosynthesis, shorter plant height and stay‐green traits. The stomatal and nonstomatal limitations are strongly associated with genotype yield performance, elucidating a potential mechanism underlying drought tolerance. Drought tolerance and yield stability of wheat cultivars have been improved through breeding over the past 70 years. Our findings enhance understanding of drought tolerance and identify genotypes and traits beneficial for breeding high‐yielding and drought‐tolerant cultivars. [ABSTRACT FROM AUTHOR]

Rhizosphere microbial communities play a substantial role in plant productivity. We studied the rhizosphere bacteria and fungi of 51 distinct potato cultivars grown under similar greenhouse conditions using a metabarcoding approach. As expected, individual cultivars were the most important determining factor of the rhizosphere microbial composition; however, differences were also obtained when grouping cultivars according to their growth characteristics. We showed that plant growth characteristics were related to deterministic and stochastic assembly processes of bacterial and fungal communities, respectively. The bacterial genera Arthrobacter and Massilia (known to produce indole acetic acid and siderophores) exhibited greater relative abundance in high- and medium-performing cultivars. Bacterial co-occurrence networks were larger in the rhizosphere of these cultivars and were characterized by a distinctive combination of plant beneficial Proteobacteria and Actinobacteria along with a module of diazotrophs namely Azospira, Azoarcus , and Azohydromonas. Conversely, the network within low-performing cultivars revealed the lowest nodes, hub taxa, edges density, robustness, and the highest average path length resulting in reduced microbial associations, which may potentially limit their effectiveness in promoting plant growth. Our findings established a clear pattern between plant productivity and the rhizosphere microbiome composition and structure for the investigated potato cultivars, offering insights for future management practices. [ABSTRACT FROM AUTHOR]

The accumulation of salt in arable lands is a source of significant abiotic stress, contributing to a 10% decline in the world's total arable lands and threatening food productivity and the sustainability of agriculture. About 76 million hectares of productive land are estimated to have been affected by human-induced salinization such as extreme salt deposits in soil, which are mainly caused by the actions of humans. For instance, continued irrigation and the frequent use of chemical fertilizers need to be understood. To ensure food availability, it is essential to improve upon traditional farming methods using current technologies to facilitate the reclamation of saline-affected arable lands to achieve high and sustainable food production. This review details current innovative strategies such as the modification of metabolic pathways, manipulation of antioxidant pathways, genetic engineering, RNA interference technology, engineered nanoparticles, arbuscular mycorrhizal fungi (AMF), organic amendments, and trace elements for improving saline marginal lands. These strategies were identified to have contributed to the improvement of plants salinity tolerance in diverse ways. For instance, the accumulation of plant metabolites such as amino acids, sugars, polyols, organic acids, saponins, anthocyanins, polyphenols, and tannins detoxify plants and play crucial roles in mitigating the detrimental effects of oxidative damage posed by salinity stress. Multiple plant miRNAs encoding the up- and down-regulation of single- and multi-ion transporters have been engineered in plant species to enhance salt tolerance. Nanomaterials and plant root system colonized by arbuscular mycorrhizal increase water uptake, photosynthetic efficiency, and biomass allocation in plants exposed to saline stress by excluding 65 percent of the Na+ uptake and enhancing K+ uptake by 84.21 percent. Organic amendments and trace elements reduced salinity concentrations by 22 percent and improved growth by up to 84 percent in maize subjected to salinity stress. This study also discusses how researchers can use these strategies to improve plants growth, development, and survival in saline soil conditions to enhance the productivity and sustainability of agriculture. The strategies discussed in this study have also proven to be promising approaches for developing salinity stress tolerance strategies for plants to increase agricultural productivity and sustainability. [ABSTRACT FROM AUTHOR]

Highlights: The spectral characteristics of different types of white LEDs differ significantly. The Phyto-LED spectrum increases the productivity of lettuce plants The combination of white LEDs and Phyto-LEDs had the same effect on the productivity of lettuce plants as the Phyto-LEDs alone. The combination of white LEDs and Phyto-LEDs has a significantly greater effect on color rendering than does the use of sole Phyto-LED spectrum Phyto-LEDs provoke flowering of lettuce plants The effect of light of various spectral compositions on the complex morphophysiological parameters of lettuce plants in hydroponic was studied. The light sources had the following light spectra: warm white light—2700 K, cold white light—6500 K, and Phyto-LED light, as well as 2700 K + Phyto-LED and 6500 K + Phyto-LED. The dry and fresh biomass, leaf area, stem length, photosynthetic pigment content, photosynthesis and transpiration rates, chlorophyll fluorescence parameters, and percentage of plants that passed into the generative stage of development were studied. The results showed that partial and complete replacement of white LEDs by Phyto-LEDs with lower green light content and greater amounts of far-red light in the radiation spectrum caused an increase in plant productivity of 37%, average leaf area, and transpiration rate in the treatments but also promoted an earlier transition of plants to flowering under light treatment, Phyto-LEDs, and Phyto-LEDs + white LEDs. The 2700 K + Phyto-LED treatment had one of the highest productivities, as did the Phyto-LED and 6500 K + Phyto-LED treatments, but this lighting treatment provoked less flowering on the 60th day of the growing period. [ABSTRACT FROM AUTHOR]

Biomass serves as a crucial indicator of plant productivity, and the development of biomass models has become an efficient way for estimating tree biomass production rapidly and accurately. This study aimed to develop a rapid and accurate model to estimate the individual aboveground biomass of Salix suchowensis. Growth parameters, including plant height (PH), ground diameter (GD), number of first branches (NFB), number of second branches (NSB) and aboveground fresh biomass weight (FW), were measured from 892 destructive sample trees. Correlation analysis indicated that GD had higher positive correlations with FW than PH, NFB and NSB. According to the biological features and field observations of S. suchowensis, the samples were classified into three categories: single-stemmed type, first-branched type and second-branched type. Based on the field measurement data, regression models were constructed separately between FW and each growth trait (PH, GD, NFB and NSB) using linear and nonlinear regression functions (linear, exponential and power). Then, multiple power regression and multiple linear regression were conducted to estimate the fresh biomass of three types of S. suchowensis. For the single-stemmed plant type, model M1 with GD as the single parameter had the highest adj R2, outperforming the other models. Among the 16 constructed biomass-estimating equations for the first-branched plant type, model M32 FW = 0.010371 × PH1.15862 × GD1.250581 × NFB0.190707 was found to have the best fit, with the highest coefficient of determination (adj R2 = 0.6627) and lowest Akaike Information Criterion (AIC = 5997.3081). When it comes to the second-branched plant type, the best-fitting equation was proved to be the multiple power model M43 with PH, GD, NFB and NSB as parameters, which had the highest adj R2 value and best-fitting effect. The stability and reliability of the models were confirmed by the F-test, repeated k-fold cross-validation and paired-sample t-tests. The models developed in this study could provide efficient tools for accurately estimating the total aboveground biomass for S. suchowensis at individual tree levels. The results of this study could also be useful for optimizing the economic productivity of shrub willow plantations. [ABSTRACT FROM AUTHOR]

Arbuscular mycorrhizal fungi (AMF) are widespread obligate symbionts of plants. This dynamic symbiosis plays a large role in successful plant performance, given that AMF help to ameliorate plant responses to abiotic and biotic stressors. Although the importance of this symbiosis is clear, less is known about what may be driving this symbiosis, the plant's need for nutrients or the excess of plant photosynthate being transferred to the AMF, information critical to assess the functionality of this relationship. Characterizing the AMF community along a natural plant productivity gradient is a first step in understanding how this symbiosis may vary across the landscape. We surveyed the AMF community diversity at 12 sites along a plant productivity gradient driven by soil nitrogen availability. We found that AMF diversity in soil environmental DNA significantly increased along with the growth of the host plants Acerrubrum and A. saccharum., a widespread tree genus. These increases also coincided with a natural soil inorganic N availability gradient. We hypothesize photosynthate from the increased tree growth is being allocated to the belowground AMF community, leading to an increase in diversity. These findings contribute to understanding this complex symbiosis through the lens of AMF turnover and suggest that a more diverse AMF community is associated with increased host–plant performance. [ABSTRACT FROM AUTHOR]