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The Hydra nervous system is the paradigm of a simple nerve net. Nerve cells in Hydra, as in many cnidarian polyps, are organized in a nerve net extending throughout the body column. This nerve net is required for control of spontaneous behavior: elimination of nerve cells leads to polyps that do not move and are incapable of capturing and ingesting prey (Campbell, 1976). We have re-examined the structure of the Hydra nerve net by immunostaining fixed polyps with a novel antibody that stains all nerve cells in Hydra. Confocal imaging shows that there are two distinct nerve nets, one in the ectoderm and one in the endoderm, with the unexpected absence of nerve cells in the endoderm of the tentacles. The nerve nets in the ectoderm and endoderm do not contact each other. High-resolution TEM (transmission electron microscopy) and serial block face SEM (scanning electron microscopy) show that the nerve nets consist of bundles of parallel overlapping neurites. Results from transgenic lines show that neurite bundles include different neural circuits and hence that neurites in bundles require circuit-specific recognition. Nerve cell-specific innexins indicate that gap junctions can provide this specificity. The occurrence of bundles of neurites supports a model for continuous growth and differentiation of the nerve net by lateral addition of new nerve cells to the existing net. This model was confirmed by tracking newly differentiated nerve cells.

Abstract We recently showed that the gain of the pupillary light response depends on numerosity, with weaker responses to fewer items. Here we show that this effect holds when the stimuli are physically identical but are perceived as less numerous due to numerosity adaptation. Twenty-eight participants adapted to low (10 dots) or high (160 dots) numerosities and subsequently watched arrays of 10–40 dots, with variable or homogeneous dot size. Luminance was constant across all stimuli. Pupil size was measured with passive viewing, and the effects of adaptation were checked in a separate psychophysical session. We found that perceived numerosity was systematically lower, and pupillary light responses correspondingly smaller, following adaptation to high rather than low numerosities. This is consistent with numerosity being a primary visual feature, spontaneously encoded even when task irrelevant, and affecting automatic and unconscious behaviours like the pupillary light response.

Takotsubo or stress cardiomyopathy is a syndrome of transient left ventricular systolic dysfunction seen in the absence of obstructive coronary artery disease. We describe a case of stress cardiomyopathy diagnosed in the emergency department (ED) using point of care ultrasound (POCUS) associated with traumatic hand amputation.  The patient suffered a near-complete amputation of the right hand while using a circular saw, subsequently complicated by brief cardiac arrest with rapid return of spontaneous circulation. Point-of-care ultrasonography in the ED revealed the classic findings of takotsubo cardiomyopathy, including apical ballooning of the left ventricle and hyperkinesis of the basal walls with a severely reduced ejection fraction. After formalization of the amputation and cardiovascular evaluation, the patient was discharged from the hospital in stable condition ten days later. Emergency physicians should be aware of the possibility of stress cardiomyopathy as a cause for acute decompensation, even in isolated extremity trauma.

There has been demand in many countries for the establishment of small campuses in more rural locations to spread the benefits of higher education both through the provision of university courses and through the positive economic spill-overs for these communities. Evaluations of the impacts of these universities according to current models show limited effects due to their small scale and specialization. Yet whilst there are clearly spill-over benefits from rural campuses into local communities, these are not only of the traditional (knowledge and economic) variety. Rather, regional campuses create social infrastructure that supports these places' quality of life. This article seeks to develop a proposal for how such social impacts of regional campuses could be evaluated by creating a conceptual framework that articulates how university-region learning communities contribute to socio-economic development trajectories of rural regions. Our overarching hypothesis is that social rural campuses are places where local learning communities work with globally sourced knowledge to make it useful and usable in particular local contexts. Over time, these activities form the basis of regular contact networks, and the benefits they bring become woven into the provision of place-specific welfare services. As a result, the university's contributions play a more structural role, and the students are involved in creating more lasting benefits by providing the interaction underpinning these structural collaborations. Our model is exemplified through an exploration of the context of the status of rural university campuses in Norway, and a case study of the Academy of Music, an outpost of the multi-campus University of Tromsø (UiT The Arctic University of Norway).

Animal genomes show networks of deeply conserved gene linkages whose phylogenetic scope and chromosomal context remain unclear. Here, we report chromosome-scale conservation of synteny among bilaterians, cnidarians, and sponges and use comparative analysis to reconstruct ancestral chromosomes across major animal groups. Comparisons among diverse metazoans reveal the processes of chromosome evolution that produced contemporary karyotypes from their Precambrian progenitors. On the basis of these findings, we introduce a simple algebraic representation of chromosomal change and use it to establish a unified systematic framework for metazoan chromosome evolution. We find that fusion-with-mixing, a previously unappreciated mode of chromosome change, has played a central role. We find that relicts of several metazoan chromosomal units are preserved in unicellular eukaryotes. These conserved pre-metazoan linkages include the chromosomal unit that encodes the most diverse set of metazoan homeobox genes, suggesting a candidate genomic context for the early diversification of this key gene family.

With Bacillus species, about 30% of extracellular proteins are translocated through the cytoplasmic membrane, coordinated by the Sec translocase. This system mainly consists of the cytoplasmic ATPase SecA and the membrane-embedded SecYEG channel. The purpose of this work was to investigate the effects of the SecYEG export system on the production of industrial biomolecules, such as biosurfactants, proteases, amylases, and cellulases. Fifty-two isolates of Bacillus species were obtained from traditional fermented foods and then characterized using molecular microbiology methods. The isolates secreted exoenzymes that included cellulases, amylases, and proteases. We present evidence that a biosurfactant-like molecule requires the SecA ATPase and the SecYEG membrane channel for its secretion. In addition, we showed that biomolecules involved in biofilm formation required the SecYEG pathway. This work presents a novel seven-target fragment multiplex PCR assay capable of identification at the species level of Bacillus through a unique SecDF chromosomal gene. The bacterial membrane protein SecDF allowed the discrimination of Bacillus subtilis, B. licheniformis, B. amyloliquefaciens, and B. sonorensis. SecA was able to interact with AprE, AmyE, and TasA. The Rose Bengal inhibitor of SecA crucially affected the interaction of AprE, AmyE, TapA, and TasA with recombinant Gst-SecA. The Rose Bengal prevented Bacillus species from secreting and producing proteases, cellulases, amylases, and biosurfactant-like molecules. It also inhibited the formation of biofilm cell communities. The data support, for the first time, that the SecYEG translocon mediates the secretion of a biosurfactant-like molecule.

Individuals with autism spectrum disorder are thought to have a more local than global perceptual style. We used a novel paradigm to investigate how grouping-induced response biases in numerosity judgments depend on autistic-like personality traits in neurotypical adults. Participants judged the numerosity of clouds of dot-pairs connected by thin lines, known to cause underestimation of numerosity. The underestimation bias correlated strongly with autism-spectrum quotient (r = 0.72, Bayes factor > 100), being weaker for participants with high autistic traits. As connecting dots probably activates global grouping mechanisms, causing dot-pairs to be processed as an integrated whole rather than as individual dots, the results suggest that these grouping mechanisms may be weaker in individuals self-reporting high levels of autistic-like traits.

Visual crowding is a phenomenon where objects presented in the visual periphery are not resolved efficiently. Here the authors show that crowding may derive from an optimizing strategy that blends information when it is similar and preserves it when it is dissimilar.

Acinar-to-ductal metaplasia (ADM) is crucial in the development of pancreatic ductal adenocarcinoma. However, our understanding of the induction and resolution of ADM remains limited. We conducted comparative transcriptome analyses to identify conserved mechanisms of ADM in mouse and human. We identified Sox4 among the top up-regulated genes. We validated the analysis by RNA in situ hybridization. We performed experiments in mice with acinar-specific deletion of Sox4 (Ptf1a: CreER; Rosa26 –LSL–YFPLSL–YFP ; Sox4 fl/fl ) with and without an activating mutation in Kras (Kras LSL–G12D/+ ). Mice were given caerulein to induce pancreatitis. We performed phenotypic analysis by immunohistochemistry, tissue decellularization, and single-cell RNA sequencing. We demonstrated that Sox4 is reactivated in ADM and pancreatic intraepithelial neoplasias. Contrary to findings in other tissues, Sox4 actually counteracts cellular dedifferentiation and helps maintain tissue homeostasis. Moreover, our investigations unveiled the indispensable role of Sox4 in the specification of mucin-producing cells and tuft-like cells from acinar cells. We identified Sox4- dependent non-cell-autonomous mechanisms regulating the stromal reaction during disease progression. Notably, Sox4-inferred targets are activated upon KRAS inactivation and tumor regression. Our results indicate that our transcriptome analysis can be used to investigate conserved mechanisms of tissue injury. We demonstrate that Sox4 restrains acinar dedifferentiation and is necessary for the specification of acinar-derived metaplastic cells in pancreatic injury and cancer initiation and is activated upon Kras ablation and tumor regression in mice. By uncovering novel potential strategies to promote tissue homeostasis, our findings offer new avenues for preventing the development of pancreatic ductal adenocarcinoma. [Display omitted] We have revealed the existence of a genetic program that promotes pancreatic healing in the context of pancreatitis, cancer initiation, and after Kras ablation–induced tumor regression. [ABSTRACT FROM AUTHOR]

The rapid growth in population, urbanization, and industrialization reduces the natural green space environment and increases air pollution on the planet. The green space environment minimizes air pollutants, and swift climate changes and provides fresh and clean air. This study investigates the prevalence of diabetes mellitus in environmentally highly green and less green countries in Europe. Based on the Environmental Performance Index (EPI) score, which rates a country's environment, 43 European countries with 760 million people were selected. The EPI score was recorded from the USA's Environmental Performance Index (EPI) website. 60% of the EPI score is based on seven categories related to ecosystem vitality: biodiversity and habitat, ecosystem, fisheries, climate change, pollution emissions, agriculture, and water resources. 40% of the score is based on four categories related to environmental health: air quality, sanitation and drinking water, heavy metals, and waste management system. The prevalence of diabetes mellitus in all 43 European countries was obtained from the International Diabetes Federation (IDF). The European countries' populations were obtained from the World Bank. We divided the countries into three groups based on each country's EPI score. The 11 countries with an EPI score of more than 75 were classified as highly green space, 24 countries with an EPI score of 50–75 were classified as moderately green space, and 8 countries with an EPI score of less than 50 were classified as less green space environment countries in Europe. The mean prevalence of diabetes mellitus for highly green space environment countries was 7.49 ± 1.53%; moderately green space countries were 8.98 ± 2.38%, and for less green space environment countries was 9.79 ± 3.05%. In countries with less green space (i.e., environment, EPI scores less than 50), the prevalence of DM was significantly higher than in green space countries with EPI scores of more than 75 (p = 0.041). The results revealed a negative correlation between the green space environment (EPI Score) and the prevalence of diabetes mellitus. The prevalence of diabetes mellitus is significantly reduced in environmentally high green space countries than in low green space countries in Europe. This means that countries with environmentally sustainable environment policies have less diabetes. It is suggested to establish strategies to keep the living environment green and clean to minimize air pollution and fight against diabetes mellitus. [ABSTRACT FROM AUTHOR]

Abstract Introduction Antimicrobial resistance (AMR) is a global public health challenge requiring a global response to which Australia has issued a National Antimicrobial Resistance Strategy. The necessity for continued-development of new effective antimicrobials is required to tackle this immediate health threat is clear, but current market conditions may undervalue antimicrobials. We aimed to estimate the health-economic benefits of reducing AMR levels for drug-resistant gram-negative pathogens in Australia, to inform health policy decision-making. Methods A published and validated-dynamic health economic model was adapted to the Australian setting. Over a 10-year time horizon, the model estimates the clinical and economic outcomes associated with reducing current AMR levels, by up to 95%, of three gram-negative pathogens in three hospital-acquired infections, from the perspective of healthcare payers. A willingness-to-pay threshold of AUD$15,000—$45,000 per quality-adjusted life-year (QALY) gained and a 5% discount rate (for costs and benefits) were applied. Results Over ten years, reducing AMR for gram-negative pathogens in Australia is associated with up to 10,251 life-years and 8924 QALYs gained, 9041 bed-days saved and 6644 defined-daily doses of antibiotics avoided. The resulting savings are estimated to be $10.5 million in hospitalisation costs, and the monetary benefit at up to $412.1 million. Discussion Our results demonstrate the clinical and economic value of reducing AMR impact in Australia. Of note, since our analysis only considered a limited number of pathogens in the hospital setting only and for a limited number of infection types, the benefits of counteracting AMR are likely to extend well beyond the ones demonstrated here. Conclusion These estimates demonstrate the consequences of failure to combat AMR in the Australian context. The benefits in mortality and health system costs justify consideration of innovative reimbursement schemes to encourage the development and commercialisation of new effective antimicrobials.

The demand of green hydrogen, that is, the hydrogen produced from water electrolysis, is expected to increase dramatically in the coming years. State‐of‐the‐art proton exchange membrane water electrolysis (PEMWE) uses high loadings of platinum group metals, such as Pt in the electrode where hydrogen is produced. Alternative electrodes based on phosphides, sulfides, nitrides, and other low‐cost alternatives are under investigation. Herein, a simple process for the preparation of RuP electrodes with high activity for the hydrogen evolution reaction (HER) in acidic electrolyte is described. A straightforward one‐pot synthesis that yields RuP nanoparticles with fine‐tuned composition and stoichiometry is presented, as determined by multiple characterization techniques, including lab‐ and synchrotron‐based experiments and theoretical modeling. The RuP nanoparticles exhibit a high activity of 10 mA cm−2 at 36 mV overpotential and a Tafel slope of 30 mV dec−1, which is comparable to Pt/C. Moreover, a RuP catalyst‐coated membrane (CCM) with a low Ru loading of 0.6 mgRu cm−2 is produced and tested in a PEMWE cell configuration, yielding 1.7 A cm−2 at 2 V.

The lignification of plant secondary walls is an important process that provides plants with mechanical support. However, the presence of lignin in the secondary walls affects the readily availability of cellulose required in various industries, including the biofuel, paper, and textile industries. Thus, plants with less lignin are ideal for usage in such industries. Molecular studies have identified genes that regulate plant lignification, including group III plant-specific patatin-related phospholipase genes. Recent studies have reported decreased lignin content when pPLAIIIα, pPLAIIIγ (from Arabidopsis thaliana), and pPLAIIIβ (from Panax ginseng) were overexpressed in Arabidopsis. However, the role played by a closely related gene pPLAIIIδ in lignin biosynthesis has not yet been reported. In this study, we found that overexpression of the pPLAIIIδ significantly reduced the lignin content in secondary cell walls, whereas the silencing of the gene increased secondary walls lignification. Transcript level analysis showed that the key structural and regulatory genes involved in the lignin biosynthesis pathway decreased in overexpression, and increased in plants with silenced pPLAIIIδ. Further analysis revealed that pPLAIIIδ played an influential role in several physiological processes including seed germination, and chlorophyll accumulation. Moreover, the gene also influenced the size of plants and plant organs, including leaves, seeds, and root hairs. Generally, our study provides important insights toward the use of genetic engineering for lignin reduction in plants and provides information about the agronomical and physiological suitability of pPLAIIIδ transgenic plants for utilization in biomass processing industries.

Introduction: Telemedicine is a valuable tool to increase access to health care, especially for patients in rural areas who need to visit a specialist. In place of telemedicine robots, which are costly and complicated, hospitals have implemented successful telemedicine programs using lower-cost tablet technology; opting for tablet technology increases the organizational feasibility of a large-scale telemedicine program. Methods: Vanderbilt University Medical Center (VUMC), in Nashville, Tennessee, USA, launched its teleneurology network program in 2014 to serve patients in surrounding community hospitals who needed a neurology consult. Consults are conducted using an iPad, including examinations and the secure sharing of images and patient information. This article reports on teleneurology consult data and the results of patient and physician satisfaction surveys. Results: Between February 2014 and November 2021, the VUMC teleneurology network program provided consultations for 14 241 patients with a wide variety of neurological diagnoses presenting to 12 community-based hospitals. Patient and community physician satisfaction surveys showed that 96% of physicians were satisfied with the overall care provided, and 89% of patients reported that the telehealth visits met their medical needs. Conclusion: One of the goals of telemedicine programs is to increase access to care. Therefore, it is important that the technology used to implement the program also be accessible in terms of cost and complexity. Tablets are low-cost technology, and their use in telemedicine has been shown to satisfy both physicians and patients with a wide variety of diagnoses.

Objectives: The rapid growth in population, urbanization, and industrialization reduces the natural green space environment and increases air pollution on the planet. The green space environment minimizes air pollutants, and swift climate changes and provides fresh and clean air. This study investigates the prevalence of diabetes mellitus in environmentally highly green and less green countries in Europe. Subjects and methods: Based on the Environmental Performance Index (EPI) score, which rates a country’s environment, 43 European countries with 760 million people were selected. The EPI score was recorded from the USA's Environmental Performance Index (EPI) website. 60% of the EPI score is based on seven categories related to ecosystem vitality: biodiversity and habitat, ecosystem, fisheries, climate change, pollution emissions, agriculture, and water resources. 40% of the score is based on four categories related to environmental health: air quality, sanitation and drinking water, heavy metals, and waste management system. The prevalence of diabetes mellitus in all 43 European countries was obtained from the International Diabetes Federation (IDF). The European countries' populations were obtained from the World Bank. We divided the countries into three groups based on each country's EPI score. The 11 countries with an EPI score of more than 75 were classified as highly green space, 24 countries with an EPI score of 50–75 were classified as moderately green space, and 8 countries with an EPI score of less than 50 were classified as less green space environment countries in Europe. Results: The mean prevalence of diabetes mellitus for highly green space environment countries was 7.49 ± 1.53%; moderately green space countries were 8.98 ± 2.38%, and for less green space environment countries was 9.79 ± 3.05%. In countries with less green space (i.e., environment, EPI scores less than 50), the prevalence of DM was significantly higher than in green space countries with EPI scores of more than 75 (p = 0.041). The results revealed a negative correlation between the green space environment (EPI Score) and the prevalence of diabetes mellitus. Conclusions: The prevalence of diabetes mellitus is significantly reduced in environmentally high green space countries than in low green space countries in Europe. This means that countries with environmentally sustainable environment policies have less diabetes. It is suggested to establish strategies to keep the living environment green and clean to minimize air pollution and fight against diabetes mellitus.