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Your search keyword '"Ostojic, Sanja"' showing total 6 results
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6 results on '"Ostojic, Sanja"'

2. Pentadecapeptide BPC 157 as Therapy for Inferior Caval Vein Embolization: Recovery of Sodium Laurate-Post-Embolization Syndrome in Rats

Author

Smoday, Ivan Maria, primary, Krezic, Ivan, additional, Kalogjera, Luka, additional, Vukovic, Vlasta, additional, Zizek, Helena, additional, Skoro, Marija, additional, Kovac, Katarina Kasnik, additional, Vranes, Hrvoje, additional, Barisic, Ivan, additional, Sikiric, Suncana, additional, Strbe, Sanja, additional, Tepes, Marijan, additional, Oroz, Katarina, additional, Zubcic, Slavica, additional, Stupnisek, Mirjana, additional, Beketic Oreskovic, Lidija, additional, Kavelj, Ivana, additional, Novosel, Luka, additional, Prenc, Matea, additional, Barsic Ostojic, Sanja, additional, Dobric, Ivan, additional, Sever, Marko, additional, Blagaic, Alenka Boban, additional, Skrtic, Anita, additional, Staresinic, Mario, additional, Sjekavica, Ivica, additional, Seiwerth, Sven, additional, and Sikiric, Predrag, additional

Published
2023
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3. A novel and effective natural product-based immunodetection tool for TNT-like compounds

Author

Simonović, Mladen, Ostojic, Sanja, Micic, Darko, Bisercic-Savić, Marjetka, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, Simonović, Mladen, Ostojic, Sanja, Micic, Darko, Bisercic-Savić, Marjetka, Mix, Thorsten, Glumac, Miodrag, and Pejin, Boris

Abstract

This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .

Published
2022

6. A novel and effective natural product-based immunodetection tool for TNT-like compounds.

Author

Simonovic M, Ostojic S, Micic D, Bisercic-Savic M, Mix T, Glumac M, and Pejin B

Subjects
Enzyme-Linked Immunosorbent Assay, Biological Products
Abstract

This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a β-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-β-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (β-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 ± 2 fmol or 157 ± 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose.

Published
2022
Full Text
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