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According to the fourth revised edition of the world health organization classification of hematopoietic tumors, methotrexate (MTX)-associated lymphoproliferative disorder (MTX-LPD), an atypical pathological condition, is categorized under other iatrogenic immunodeficiency-associated lymphoproliferative disorders (OIIA-LPDs). An 80-year-old woman presented with OIIA-LPDs that simultaneously occurred in the gingiva and intestinal mucosa, causing intestinal perforation requiring emergency surgery. She was referred to our hospital because of poor wound healing after maxillary right first molar extraction. She had been prescribed MTX and prednisolone for rheumatoid arthritis and bisphosphonate for osteoporosis. We diagnosed her with medication-related osteonecrosis of the jaw, as the lesion showed bone exposure with mild redness and swelling of the surrounding gingiva and a cytological result of Class II. However, 9 months later, following the exfoliation of a partial sequestrum of the maxillary right first molar, a rapidly growing mass with gingival necrosis appeared, necessitating a biopsy. Simultaneously, abdominal pain worsened, and computed tomography revealed intestinal perforation; an emergency laparotomy was performed to remove the perforation and the surrounding ulcer-prone area. Histopathological examination of both lesions revealed diffuse large B-cell lymphomas. The final diagnosis was of MTX-LPD, as the mass, gingival necrosis, and residual small intestinal ulcer disappeared 2 months after MTX discontinuation. The exposed bone completely disappeared after 24 months, and no recurrence was noted after 60 months following MTX discontinuation. Although intestinal perforation due to MTX-LPD is extremely rare, patients with RA are immunosuppressed. Therefore, MTX-LPD must be recognized as a dangerous condition that requires careful general monitoring in such patients. [ABSTRACT FROM AUTHOR]

The usefulness and effectiveness of telepractice have been reported in recent years. Treatment of cleft palate patients with compensatory articulation is based on perceptual identification. Telepractice using videoconferencing platforms causes voice signal distortion and impacts auditory-perceptual perception. This study aimed acoustically examine voice signal distortion and determine the optimal videoconferencing platforms, in addition to the phonemes that can be discriminated with the same quality as in face-to-face interactions. ATR503 with 50 phoneme-balanced Japanese speech sentences was used as a reference corpus. Four videoconferencing platforms, -Zoom, Cisco Webex, Skype, and Google Meet, -and five devices, -iPhone, Android, iPad Air, Windows, and MacBook Pro were used as transmission conditions to examine voice signal distortions with the objective measure log-spectral distortion (LSD). Tukey's test was conducted to evaluate the degree of consonant distortion related to voicings (voiceless and voiced), places of articulation (bilabial, alveolar, alveolo-palatal, palatal, velar, labial-velar, and glottal), and manners of articulation (plosive, fricative, affricate, tap or flap, nasal, and approximant). With statistically significant differences, voiced, bilabial, labial-velar, nasal, and plosive consonants exhibited smaller distortions. In contrast, voiceless, alveolo-palatal, fricative, and affricate consonants exhibited larger distortions. Google Meet exhibited the lowest distortion among videoconferencing platforms and MacBook exhibited the lowest distortion among devices. This study provides significant insights into the telepractice strategies with the appropriate videoconferencing platform and device, and useful settings for cleft palate patients with compensatory articulations with respect to acoustics., (© 2024 The Author(s). Congenital Anomalies published by John Wiley & Sons Australia, Ltd on behalf of Japanese Teratology Society.)

Background/aim: Bone resolution due to tumor invasion often occurs on the surface of the jaw and is important for clinical prognosis. Although cytokines, such as TNF-α are known to impair osteoblasts, the underlying mechanism remains unclear. Protein myristoylation, a post-translational modification, plays an important role in the development of immune responses and cancerization of cells. A clear understanding of the mechanisms underlying this involvement will provide insights into molecular-targeted therapies. N-myristoyltransferase1 (NMT1), a specific enzyme involved in myristoylation, is expressed in cancer cells and in other normal cells, suggesting that changes in myristoylation may result from the regulation of NMT1 in cancer cells., Materials and Methods: Using newly emerging state-of-the-art techniques such as the Click-it assay, RNA interference, mass spectrometry, immunoprecipitation, immunocytochemistry, and western blotting, the expression of myristoylated proteins and the role of TNF-α stimulation on NMT1 and Sorbs2 binding were evaluated in a murine osteoblastic cell line (MC3T3-E1)., Results: The expression of myristoylated proteins was detected; however, TNF-α stimulation resulted in their inhibition in MC3T3-E1 cells. The expression of NMT1 also increased. Immunoprecipitation and mass spectrometry identified Sorbs2 as a novel binding protein of NMT1, which upon TNF-α stimulation, inhibited myristoylation., Conclusion: The binding between NMT1 and Sorbs2 can regulate myristoylation, and NMT1 can be considered as a potential target molecule for tumor invasion., (Copyright © 2024, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)