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5. Interplay between calcium and sarcomeres directs cardiomyocyte maturation during regeneration

Author

Nguyen, Phong D., primary, Gooijers, Iris, additional, Campostrini, Giulia, additional, Verkerk, Arie O., additional, Honkoop, Hessel, additional, Bouwman, Mara, additional, de Bakker, Dennis E. M., additional, Koopmans, Tim, additional, Vink, Aryan, additional, Lamers, Gerda E. M., additional, Shakked, Avraham, additional, Mars, Jonas, additional, Mulder, Aat A., additional, Chocron, Sonja, additional, Bartscherer, Kerstin, additional, Tzahor, Eldad, additional, Mummery, Christine L., additional, de Boer, Teun P., additional, Bellin, Milena, additional, and Bakkers, Jeroen, additional

Published
2023
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6. Interplay between calcium and sarcomeres directs cardiomyocyte maturation during regeneration

Author

Nguyen, Phong D, Gooijers, Iris, Campostrini, Giulia, Verkerk, Arie O, Honkoop, Hessel, Bouwman, Mara, de Bakker, Dennis E M, Koopmans, Tim, Vink, Aryan, Lamers, Gerda E M, Shakked, Avraham, Mars, Jonas, Mulder, Aat A, Chocron, Sonja, Bartscherer, Kerstin, Tzahor, Eldad, Mummery, Christine L, de Boer, Teun P, Bellin, Milena, Bakkers, Jeroen, Nguyen, Phong D, Gooijers, Iris, Campostrini, Giulia, Verkerk, Arie O, Honkoop, Hessel, Bouwman, Mara, de Bakker, Dennis E M, Koopmans, Tim, Vink, Aryan, Lamers, Gerda E M, Shakked, Avraham, Mars, Jonas, Mulder, Aat A, Chocron, Sonja, Bartscherer, Kerstin, Tzahor, Eldad, Mummery, Christine L, de Boer, Teun P, Bellin, Milena, and Bakkers, Jeroen

Abstract

Zebrafish hearts can regenerate by replacing damaged tissue with new cardiomyocytes. Although the steps leading up to the proliferation of surviving cardiomyocytes have been extensively studied, little is known about the mechanisms that control proliferation and redifferentiation to a mature state. We found that the cardiac dyad, a structure that regulates calcium handling and excitation-contraction coupling, played a key role in the redifferentiation process. A component of the cardiac dyad called leucine-rich repeat-containing 10 (Lrrc10) acted as a negative regulator of proliferation, prevented cardiomegaly, and induced redifferentiation. We found that its function was conserved in mammalian cardiomyocytes. This study highlights the importance of the underlying mechanisms required for heart regeneration and their application to the generation of fully functional cardiomyocytes.

Published
2023

7. Interplay between calcium and sarcomeres directs cardiomyocyte maturation during regeneration

Author

Medische Fysiologie, Pathologie Pathologen staf, Circulatory Health, Child Health, Onderzoek, Cardiologie onderzoek 2, Nguyen, Phong D, Gooijers, Iris, Campostrini, Giulia, Verkerk, Arie O, Honkoop, Hessel, Bouwman, Mara, de Bakker, Dennis E M, Koopmans, Tim, Vink, Aryan, Lamers, Gerda E M, Shakked, Avraham, Mars, Jonas, Mulder, Aat A, Chocron, Sonja, Bartscherer, Kerstin, Tzahor, Eldad, Mummery, Christine L, de Boer, Teun P, Bellin, Milena, Bakkers, Jeroen, Medische Fysiologie, Pathologie Pathologen staf, Circulatory Health, Child Health, Onderzoek, Cardiologie onderzoek 2, Nguyen, Phong D, Gooijers, Iris, Campostrini, Giulia, Verkerk, Arie O, Honkoop, Hessel, Bouwman, Mara, de Bakker, Dennis E M, Koopmans, Tim, Vink, Aryan, Lamers, Gerda E M, Shakked, Avraham, Mars, Jonas, Mulder, Aat A, Chocron, Sonja, Bartscherer, Kerstin, Tzahor, Eldad, Mummery, Christine L, de Boer, Teun P, Bellin, Milena, and Bakkers, Jeroen

Published
2023

8. Single-cell profiling of transcriptome and histone modifications with EpiDamID

Author

Rang, Franka J, de Luca, Kim L, de Vries, Sandra S, Valdes-Quezada, Christian, Boele, Ellen, Nguyen, Phong D, Guerreiro, Isabel, Sato, Yuko, Kimura, Hiroshi, Bakkers, Jeroen, Kind, Jop, Rang, Franka J, de Luca, Kim L, de Vries, Sandra S, Valdes-Quezada, Christian, Boele, Ellen, Nguyen, Phong D, Guerreiro, Isabel, Sato, Yuko, Kimura, Hiroshi, Bakkers, Jeroen, and Kind, Jop

Abstract

Recent advances in single-cell sequencing technologies have enabled simultaneous measurement of multiple cellular modalities, but the combined detection of histone post-translational modifications and transcription at single-cell resolution has remained limited. Here, we introduce EpiDamID, an experimental approach to target a diverse set of chromatin types by leveraging the binding specificities of single-chain variable fragment antibodies, engineered chromatin reader domains, and endogenous chromatin-binding proteins. Using these, we render the DamID technology compatible with the genome-wide identification of histone post-translational modifications. Importantly, this includes the possibility to jointly measure chromatin marks and transcription at the single-cell level. We use EpiDamID to profile single-cell Polycomb occupancy in mouse embryoid bodies and provide evidence for hierarchical gene regulatory networks. In addition, we map H3K9me3 in early zebrafish embryogenesis, and detect striking heterochromatic regions specific to notochord. Overall, EpiDamID is a new addition to a vast toolbox to study chromatin states during dynamic cellular processes.

Published
2022

9. Single-cell profiling of transcriptome and histone modifications with EpiDamID

Author

Cardiologie onderzoek 2, Onderzoek, Child Health, Circulatory Health, Rang, Franka J., de Luca, Kim L., de Vries, Sandra S., Valdes-Quezada, Christian, Boele, Ellen, Nguyen, Phong D., Guerreiro, Isabel, Sato, Yuko, Kimura, Hiroshi, Bakkers, Jeroen, Kind, Jop, Cardiologie onderzoek 2, Onderzoek, Child Health, Circulatory Health, Rang, Franka J., de Luca, Kim L., de Vries, Sandra S., Valdes-Quezada, Christian, Boele, Ellen, Nguyen, Phong D., Guerreiro, Isabel, Sato, Yuko, Kimura, Hiroshi, Bakkers, Jeroen, and Kind, Jop

Published
2022

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