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We propose a reconstruction method for coherence holography using deep neural networks. cGAN and U-NET models were developed to reconstruct 3D complex objects from recorded interferograms. Our proposed methods, dubbed deep coherence holography (DCH), predict the non-diffracted fields or the sub-objects included in the 3D object from the captured interferograms, yielding better reconstructed objects than the traditional analytical imaging methods in terms of accuracy, resolution, and time. The DCH needs one image per sub-object as opposed to N images for the traditional sin-fit algorithm, and hence the total reconstruction time is reduced by N ×. Furthermore, with noisy interferograms the DCH amplitude mean square reconstruction error (MSE) is 5×10 4 × and 10 4 × and phase MSE is 10 2 × and 3×10 3 × better than Fourier fringe and sin-fit algorithms, respectively. The amplitude peak signal to noise ratio (PSNR) is 3× and 2× and phase PSNR is 5× and 3× better than Fourier fringe and sin-fit algorithms, respectively. The reconstruction resolution is the same as sin-fit but 2× better than the Fourier fringe analysis technique.

Optical diffraction tomography (ODT) solves an inverse scattering problem to obtain label-free, 3D refractive index (RI) estimation of biological specimens. This work demonstrates 3D RI retrieval methods suitable for partially-coherent ODT systems supported by intensity-only measurements consisting of axial and angular illumination scanning. This framework allows for access to 3D quantitative RI contrast using a simplified non-interferometric technique. We consider a traditional iterative tomographic solver based on a multiple in-plane representation of the optical scattering process and gradient descent optimization adapted for focus-scanning systems, as well as an approach that relies solely on 3D convolutional neural networks (CNNs) to invert the scattering process. The approaches are validated using simulations of the 3D scattering potential for weak phase 3D biological samples.

Digital holographic microscopy is an imaging technique particularly well suited to the study of living cells in culture, as no labeling is required and computed phase maps produce high contrast, quantitative pixel information. A full experiment involves instrument calibration, cell culture quality checks, selection and setup of imaging chambers, a sampling plan, image acquisition, phase and amplitude map reconstruction, and parameter map post-processing to extract information about cell morphology and/or motility. Each step is described below, focusing on results from imaging four human cell lines. Several post-processing approaches are detailed, with an aim of tracking individual cells and dynamics of cell populations., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Multi-wavelength digital holographic microscopy (MWDHM) provides indirect measurements of the refractive index for non-dispersive samples. Successive-shot MWDHM is not appropriate for dynamic samples and single-shot MWDHM significantly increases the complexity of the optical setup due to the need for multiple lasers or a wavelength tunable source. Here we consider deep learning convolutional neural networks for computational phase synthesis to obtain high-speed simultaneous phase estimates on different wavelengths and thus single-shot estimates of the integral refractive index without increased experimental complexity. This novel, to the best of our knowledge, computational concept is validated using cell phantoms consisting of internal refractive index variations representing cytoplasm and membrane-bound organelles, respectively, and a simulation of a realistic holographic recording process. Specifically, in this work we employed data-driven computational techniques to perform accurate dual-wavelength hologram synthesis (hologram-to-hologram prediction), dual-wavelength phase synthesis (unwrapped phase-to-phase prediction), direct phase-to-index prediction using a single wavelength, hologram-to-phase prediction, and 2D phase unwrapping with sharp discontinuities (wrapped-to-unwrapped phase prediction).