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Your search keyword '"Nederlof M"' showing total 2 results
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2 results on '"Nederlof M"'

1. Schoon, zoet water in een circulaire waterketen

Author

Nederlof, M., Homeijer, N., Ramaker, M., Kiestra, F., Nederlof, M., Homeijer, N., Ramaker, M., and Kiestra, F.

Abstract

In 2050 moet Nederland volledig circulair zijn. Dat geldt ook voor de waterketen. Maar wat houdt een circulaire waterketen in? In dit artikel wordt voorgesteld bij het definiëren van doelen voor een circulaire economie 1) schoon zoet water mee te nemen als grondstof, 2) drinkwater en proceswater als product en 3) grond- en oppervlaktewater als milieu-compartiment. Daarmee wordt op weg naar 2050 zowel de beschikbaarheid van schoon zoet water geborgd voor de drinkwater- en proceswatervoorziening, als de kwaliteit van grondwater en oppervlaktewater verbeterd.

Published
2022

2. MACSima imaging cyclic staining (MICS) technology reveals combinatorial target pairs for CAR T cell treatment of solid tumors.

Author

Kinkhabwala A, Herbel C, Pankratz J, Yushchenko DA, Rüberg S, Praveen P, Reiß S, Rodriguez FC, Schäfer D, Kollet J, Dittmer V, Martinez-Osuna M, Minnerup L, Reinhard C, Dzionek A, Rockel TD, Borbe S, Büscher M, Krieg J, Nederlof M, Jungblut M, Eckardt D, Hardt O, Dose C, Schumann E, Peters RP, Miltenyi S, Schmitz J, Müller W, and Bosio A

Subjects
Cell Death, Cytotoxicity, Immunologic, High-Throughput Screening Assays, Humans, Neoplasms immunology, Neoplasms pathology, Receptors, Chimeric Antigen genetics, Receptors, Chimeric Antigen metabolism, T-Lymphocytes immunology, T-Lymphocytes metabolism, T-Lymphocytes transplantation, Biomarkers, Tumor metabolism, Epithelial Cell Adhesion Molecule metabolism, Fluorescent Antibody Technique, Immunotherapy, Adoptive, Neoplasms metabolism, Neoplasms therapy, Photobleaching, Single-Cell Analysis, Thy-1 Antigens metabolism
Abstract

Many critical advances in research utilize techniques that combine high-resolution with high-content characterization at the single cell level. We introduce the MICS (MACSima Imaging Cyclic Staining) technology, which enables the immunofluorescent imaging of hundreds of protein targets across a single specimen at subcellular resolution. MICS is based on cycles of staining, imaging, and erasure, using photobleaching of fluorescent labels of recombinant antibodies (REAfinity Antibodies), or release of antibodies (REAlease Antibodies) or their labels (REAdye_lease Antibodies). Multimarker analysis can identify potential targets for immune therapy against solid tumors. With MICS we analysed human glioblastoma, ovarian and pancreatic carcinoma, and 16 healthy tissues, identifying the pair EPCAM/THY1 as a potential target for chimeric antigen receptor (CAR) T cell therapy for ovarian carcinoma. Using an Adapter CAR T cell approach, we show selective killing of cells only if both markers are expressed. MICS represents a new high-content microscopy methodology widely applicable for personalized medicine., (© 2022. The Author(s).)

Published
2022
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