Osseous cyst-like lesions belong to the recurrent findings in the locomotor system of the horse. Their cause is still not clearly understood. Their significance in the diagnosis of lameness is also difficult to determine. The objectives of the present study were to investigate the frequency of occurrence of osseous cyst-like lesions in the equine hoof and to compare radiographic and magnetic resonance imaging findings. In addition, the characteristics of the different osseous cyst-like lesions should be presented in relation to the clinical symptoms. In this study, 195 limbs of 176 horses were analyzed. Horses with radiographic suspicion of an osseous cyst-like lesion in the coffin joint or navicular bone were included and those horses in which this was confirmed or refuted. For the control group, all horses in the period that did not show any deviating findings in radiographic and MRI examinations were also recorded. Actual osseous cyst-like lesions were present in 109 limbs of 103 horses. Group 1 included 71 limbs of 68 lame horses with osseous cyst-like lesions in the coffin bone, middle phalanx or navicular bone that had positive anesthesia. Group 2 included 27 limbs of 26 horses with osseous cyst-like lesions that did not receive anesthesia. Group 3, which included 11 limbs from 11 horses, included sound horses with osseous cyst-like lesions in the coffin bone, middle phalanx or navicular bone. Of these 11 horses, 2 horses were already included in group 1 with a lame limb. Group 4, which included horses that had a suspected osseous cyst-like lesion on radiography that was not confirmed on MRI, included 19 limbs from 18 horses. In group 5, the control group without osseous cyst-like lesions and other findings in radiographic and MRI examination, 67 limbs of 55 horses were included. The age of the horses with osseous cyst-like lesions ranged from 1 to 22 years and was normally distributed (p=0.175). The majority of the patients were Warmbloods with 86 horses (82%). 12 patients (12%) belonged to Western horses, under which Paint Horses and Quarter Horses were grouped. For sport, 38 horses (37%) of the patient stock were used. For recreational use, there were 54 horses (52%). Of 109 osseous cyst-like lesions, 104 (95%) occurred on the forelimbs. The left forelimb was affected in 46% (49 horses) and the right forelimb in 49% (52 horses) of the patients. 28% of the osseous cyst-like lesions were found in the coffin bone and most commonly midsagittally, 21% in the middle phalanx and most commonly midsagittally, and 51% in the navicular bone, most commonly at the distal border. By MRI, these could be assigned predominantly to the flexor cortex (93%). The most common form appeared circular (65%) with a sclerotic rim (94%). Among these, the osseous cyst-like lesions in the coffin joint had more frequent communications with the joint (77%) than those of the navicular bone (14%). The size of osseous cyst-like lesions in the coffin bone was often somewhat overestimated radiographically and those in the navicular bone underestimated. Osseous cyst-like lesions in the navicular bone were significantly more common in older horses (p=0.029) and in pleasure horses (p=0.02). The agreement of radiographic and MRI findings of osseous cyst-like lesions in the coffin bone could be classified as moderate (Ƙ=0.515). Thus, a sensitivity of 74.1% and a specificity of 77.9% can be determined for radiographic detection of osseous cyst-like lesions in the distal toe. 10% of horses with osseous cyst-like lesions were sound. 24% of the limbs with panesthesia had findings other than the osseous cyst-like lesion in the distal limb, so these were also omitted from the assessment of lameness severity because it could not be ruled out that the lameness was due to other findings. Lameness caused by the osseous cyst-like lesion was found in 52 limbs. The degree of lameness varied from grade 1 to grade 4. The size of osseous cyst-like lesion did not show any relation to the degree of lameness. 69% of the limbs with osseous cyst-like lesions showed bursitis in lame horses. The presence of bursitis, coffin joint filling, and deep digital flexor tendon lesion resulted in more severe lameness. Whereas the degree of sclerosis did not seem to have an effect on the degree of lameness. Overall, it can be concluded that osseous cyst-like lesions occur more frequently in the navicular bone than in other bones of the hoof. The horses are significantly older when these osseous cyst-like lesions occur. There were no correlations between lameness and characteristics of the osseous cyst-like lesions, while age and use showed correlations. [ABSTRACT FROM AUTHOR]