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En el presente artículo, se han buscado las pistas de la aplicación del Teatro del Oprimido en Oaxaca, esperando que sea un peldaño en el proceso de transformación social. Esta investigación se realizó al cursar la Maestría en Teatro y Artes Escénicas durante los años 2021 al 2022 y, tiene un alcance exploratorio y metodología mixta, donde se documentaron los eventos y procesos suscitados del 2009 al 2021.

Los animales son las principales víctimas de la historia, esto fue mencionado por primera vez en el texto descrito por Peter Singer La liberación animal. Enfocarse en una ética para el bienestar animal implica situar la mirada en la capacidad de sentir qué experimentan los animales. El bienestar animal es la capacidad de los animales de sentir dolor, sufrimiento, es decir, experimentan emociones.La experimentación animal no es un tema reciente, es un problema que ha existido desde hace muchos años. Un pilar fundamental es el comité de ética institucional, su función es evaluar cada paso antes, y durante la experimentación, verificando el programa, procedimiento, justificación, bienestar, ofrecer un digno y adecuado alojamiento que sea confortable. Cualquier experimentación con animales debe tener una justificación científica, evitar el dolor, sufrimiento, así como tener personal capacitado para salvaguardar su bienestar

Los objetivos fueron analizar la estructura y variabilidad genética del bisonte americano con marcadores genéticos de tipo SNP. Se muestrearon 174 bisontes y se analizaron 42,366 SNP distribuidos en los 29 cromosomas. Se estimó la heterocigosis esperada (He) y observada (Ho), contenido de información polimórfica (CIP), índice de fijación (FIS), índice de Shannon (IS), desequilibrio de ligamiento y relación de parentesco (Rij; %), así como el tamaño efectivo de población (Ne). Se realizó un análisis de estructura genética para inferir cuántas líneas o genomas (k) definen la población. Se identificó y seleccionó un panel con 2,135 SNP polimórficos, con un promedio de 74 SNP por cromosoma. En el proceso de exclusión, 84.5 % fueron monomórficos, 8.5 % con porcentaje de usables menor a 90 %, 6.3 % con frecuencia del alelo menor inferior a 0.01 y 0.70 % por desequilibrio Hardy-Weinberg (P

RESUMEN | La camaronicultura es una actividad en constante crecimiento, lo que ha llevado al deterioro de los ecosistemas por la deposición de efluentes que impactan al medio ambiente, generando no solo daños a los ecosistemas sino también a la misma calidad del agua que ingresa al cultivo. Esta investigación busca evaluar la eficiencia de consorcios microbianos autóctonos (in vitro) en la biorremediación del efluente de una finca camaronera ubicada en el sector Cabello Manabí, Ecuador. Para ello, se analizaron los parámetros: fosfatos, amonio, nitritos, nitratos, sólidos en suspensión, oxígeno disuelto, temperatura, pH, DBO5 y coliformes totales. Se utilizó un diseño completamente aleatorio con un factor y 4 niveles y se realizaron 4 tratamientos: consorcio bacteriano (T1) incluyendo la levadura Saccharomyces cerevisiae, consorcio de microalgas (T2), consorcio de hongos (T3) conformado por Trichoderma harzianum, S. cerevisiae, Lactobacillus acidophilus, Bacillus subtilis, Lactobacillus plantarum, y como (T4) un consorcio combinado de los tres tratamientos anteriores. Se observó una diferencia estadísticamente significativa en T2 con respecto a los demás tratamientos, pues debido a sus actividades metabólicas se logró la biorremediación de elementos como fosfatos, compuestos nitrogenados y coliformes totales, mientras que en los demás parámetros no se logró una mejora significativa. El proceso llevado a cabo por las microalgas permitió ajustar los parámetros analizados a criterios de calidad del agua para ser reutilizados en actividades agrícolas y ganaderas e incluso vertidos en cuerpos de agua dulce, concluyendo que la aplicación de microalgas autóctonas de la zona es útil para la biorremediación de los efluentes camaroneros de la localidad. ABSTRACT | Shrimp farming is a constantly growing activity, which has led to the deterioration of ecosystems due to the deposition of effluents that impact the environment, which generates not only damage to ecosystems but also to the same quality of the water that enters the cultivation area. That is why this research seeks to evaluate the efficiency of autochthonous microbial consortia (in vitro) in the bioremediation of the effluent of a shrimp farm located in the Cabello Manabí sector - Ecuador. For this, the following parameters were analyzed: phosphates, ammonium, nitrites, nitrates, suspended solids, dissolved oxygen, temperature, pH, BOD5 and total coliforms. A Completely Randomized Design with one factor and 4 levels was used, using 4 treatments: bacterial consortium (T1) including the yeast Saccharomyces cerevisiae., microalgae consortium (T2)., fungal consortium (T3) made up of Trichoderma harzianum, S. cerevisiae, Lactobacillus acidophilus, Bacillus subtilis, Lactobacillus plantarum., and as (T4) a combined consortium of the three previous treatments. A statistically significant difference was observed in T2 with respect to the other treatments, since thanks to its metabolic activities, the bioremediation of elements such as phosphates, nitrogen compounds and total coliforms was observed, while in the other parameters a significant improvement was not achieved. The process carried out by the microalgae made it possible to adjust the parameters analyzed to water quality criteria to be reused in agricultural and livestock activities and even discharged into freshwater bodies, concluding that the application of microalgae native to the area was useful for the bioremediation of shrimp effluents from the locality.

Introduction: Primary autism is a heterogeneous neurobehavioral disorder of uncertain etiology in which both genes and the environment contribute to the pathogenesis of the disorder. Objective: To identify family inherited, prenatal and perinatal risk factors in Cuban children with primary autism. Material and Methods: An observational case-control study (1:1) was carried out in children with primary autism, treated at "Juan Manuel Márquez" Pediatric Hospital, Havana, in the period from October of 2014 to September of 2019. The sample was made up of 126 cases and 126 controls. Data on neuropsychiatric diseases, prenatal and perinatal history of three generations were collected. Multivariate logistic regression was performed to identify risk factors related to primary autism. Results: The odds of presenting primary autism were approximately seven and four times higher in children of mothers and fathers of advanced ages, respectively. A history of language disorders and epilepsy in first-degree relatives conferred 27- and 24-fold higher odds of presenting with autism, respectively. The odds of presenting autism were approximately ten times greater in children born to pregnant women with anemia, eight times in children born to pregnant women who had bleeding during pregnancy, and 18 times in those born to mothers with a history of pregestational diabetes mellitus. Conclusions: The history of inherited neuropsychiatric diseases and prenatal and perinatal environmental factors related to hypoxemic events are risk factors for primary autism in the sample of Cuban children studied.

Objective To determine if inappropriate tapering/discontinuation of opioids to Alberta patients occurred from mid-2013–2020, as unintended consequences of prescribing guidelines, regulations and policies in response to the North American opioid crisis.Design A population-based, repeated cross-sectional time-series study.Setting Alberta, Canada.Participants Residents of Alberta, Canada aged 18 and older who received an opioid dispense from a community pharmacy from 2013 to 2020.Main outcome measures The prevalence of potential rapid tapering was measured at a given date (reference day), enveloped by a data window. Dose changes were measured as oral morphine equivalents (OME) per patient, at multiple time points (‘data window’ around a reference day). Chronic recipients were identified, and their prescriptions were contrasted 90 days before and after the reference day to measure OME/day changes.Results Approximately 9000 dispenses (totalling ~6 million OME) per day were analysed from 2013 to 2020. The total number of opioid recipients was highly cyclic in nature (peaking in winter). The number of chronic opioid recipients remained somewhat stable from ~70K in 2013 to ~86K at the end of 2020. The number of chronic high and very high dose recipients presented a significant decrease after 2017. Approximately 11%–12% of chronic high-dose recipients experienced potential rapid dose tapering at a rate of 50% or more prereference to postreference day at any given point of time. For chronic very high dose recipients, approximately 11.5% experience potential rapid dose tapering at a rate of 50% or more prereference to postreference day at any given point of time. Potential discontinuation remained constant and the interventions did not have a significant impact on the trend.Conclusion The evidence suggests that changes in prescribing guidelines were not associated with an increase of rapid opioid tapering/discontinuation in Alberta.

Abstract The emergence of the novel SARS-CoV-2 virus is the most important public-health issue of our time. Understanding the diverse clinical presentations of the ensuing disease, COVID-19, remains a critical unmet need. Here we present a comprehensive listing of the diverse clinical indications associated with COVID-19. We explore the theory that anti-SARS-CoV-2 antibodies could cross-react with endogenous human proteins driving some of the pathologies associated with COVID-19. We describe a novel computational approach to estimate structural homology between SARS-CoV-2 proteins and human proteins. Antibodies are more likely to interrogate 3D-structural epitopes than continuous linear epitopes. This computational workflow identified 346 human proteins containing a domain with high structural homology to a SARS-CoV-2 Wuhan strain protein. Of these, 102 proteins exhibit functions that could contribute to COVID-19 clinical pathologies. We present a testable hypothesis to delineate unexplained clinical observations vis-à-vis COVID-19 and a tool to evaluate the safety-risk profile of potential COVID-19 therapies.

Summary: Here, we describe a bioinformatics pipeline that evaluates the interactions between coagulation-related proteins and genetic variants with SARS-CoV-2 proteins. This pipeline searches for host proteins that may bind to viral protein and identifies and scores the protein genetic variants to predict the disease pathogenesis in specific subpopulations. Additionally, it is able to find structurally similar motifs and identify potential binding sites within the host-viral protein complexes to unveil viral impact on regulated biological processes and/or host-protein impact on viral invasion or reproduction.For complete details on the use and execution of this protocol, please refer to Holcomb et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.