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There is a growing body of work suggesting that social class stereotypes are amplified when people perceive higher levels of economic inequality—that is, the wealthy are perceived as more competent and assertive and the poor as more incompetent and unassertive. The present study tested this prediction in 32 societies and also examines the role of wealth-based categorization in explaining this relationship. We found that people who perceived higher economic inequality were indeed more likely to consider wealth as a meaningful basis for categorization. Unexpectedly, however, higher levels of perceived inequality were associated with perceiving the wealthy as less competent and assertive and the poor as more competent and assertive. Unpacking this further, exploratory analyses showed that the observed tendency to stereotype the wealthy negatively only emerged in societies with lower social mobility and democracy and higher corruption. This points to the importance of understanding how socio-structural features that co-occur with economic inequality may shape perceptions of the wealthy and the poor. © 2022 The Authors. European Journal of Social Psychology published by John Wiley & Sons Ltd. WOS:000880016700001 2-s2.0-85141616689 Social Sciences Citation Index Q2 Article Uluslararası işbirliği ile yapılan - EVET 2022 YÖK - 2022-23 Kasım

The purpose was to assess COVID-19 beliefs and attitudes and examine COVID-19-related changes in sexual behavior of men who have sex with men during 3 time periods: April-July 2020 (T1), August-December 2020 (T2), January-May 2021 (T3).Data were analyzed from 157 men who have sex with men in Ohio recruited to participate in a longitudinal multisite network study of syphilis epidemiology in 3 US cities: Columbus, Ohio; Baltimore, Maryland; and Chicago, Illinois. In April 2020, a COVID-19 module was appended to existing baseline and follow-up surveys to assess beliefs, attitudes, and changes in sexual behavior. Sample characteristics were summarized. Correlations between demographic variables (age, racial identity) and COVID-19 outcomes were examined.In response to COVID-19 social distancing restrictions and self-reported anxiety, some men limited sexual activity at T1, but the majority (n = 105 [67%]) continued to engage in sex. The number of men engaging in sex increased over time (T2: n = 124 [79%]; T3: n = 121 [77%]). At T1, men not in a relationship more frequently reported having less sex compared with prepandemic (n = 39 [57%]). By T3, men in a relationship more frequently reported less sex (n = 32 [54%]). Increased anxiety about sex and condom use was positively correlated with identifying as a man of color (P0.001). Most of the sample reported either starting or increasing online sexual activity during each time period.Future efforts to target sexual health during a pandemic or other health emergencies should prioritize men of color and address the unique perspective of both single and partnered men.

Pseudemys is a genus of commonly occurring freshwater turtles with limited growth information across their long lifespans. We used 11,361 mark-recapture events to estimate the somatic growth rates of P. nelsoni, P. peninsularis, P. concinna suwanniensis, and P. texana from freshwater springs and developed a Bayesian growth model to estimate the species-specific, site-specific, and individual effects on growth. We corroborated evidence for fast juvenile growth and slower adult growth in Pseudemys but found uncommonly fast growth rates, with turtles doubling or tripling in size in the first year. P. texana males had the smallest average maximum size (L∞, 243 mm), while P. c. suwanniensis females had the largest (423 mm). Environmental conditions at springs had significant effects on k, the growth coefficient, but not L∞. We derived, using a ratio of length at maturity to L∞ (71.7% and 87%, males and females), that females matured 1.15–1.57 times older than males except for P. c. suwanniensis, which matured three times older. Given the local abundance declines in many Pseudemys from anthropogenic impacts, this study provides important baseline life history information for Pseudemys species for use in ongoing conservation efforts and presents a novel hierarchical modeling approach using a long-term mark-recapture dataset.

Recent research has highlighted the potential of nostalgic recall of intergroup contact as a means of enhancing intergroup relationships and tackling prejudice. In this article, we review the scarce but promising literature that integrates research on nostalgia and intergroup contact. We outline the mechanisms that explain the link between nostalgic intergroup encounters and improved intergroup attitudes and behavior. We further highlight the benefits that nostalgic contact reverie may have for intergroup relations – and beyond. We then discuss the potential of nostalgic intergroup contact as a strategy for real-world, prejudice reduction interventions. Finally, we draw on current research from the fields of nostalgia and intergroup contact to make suggestions for future research.“… nostalgic memories … lead to a vivid sense of commonality [which] accelerates the process of acquaintance in a community where formerly only barriers may have existed.” [1, p. 454].Nostalgia, “a sentimental longing or wistful affection for the past” [ [2], p. 1266], is a bittersweet, yet predominantly positive emotion [3] that occurs frequently [4,5], and is conceptualized similarly across cultures [6∗]. An intrinsic characteristic of nostalgia is its sociality [5,7∗∗]. Indeed, many of nostalgia's positive consequences emerge as a function of meaningful past relationships that are made accessible during nostalgic reverie [8, 9, 10, 11]. In doing so, nostalgia promotes approach motivation, renders it more likely that people will perceive relationship goals as important and achievable, and enhances self-efficacy and optimism about establishing, maintaining and resolving conflict in relationships [12,13].With these consequences in mind, we argue that nostalgia has excellent potential as a means of bolstering intergroup relationships. To explicate this, we review the burgeoning literature which draws a connection between nostalgia and intergroup contact, highlight the benefits this may have for intergroup relations, and rely on research from the nostalgia and intergroup contact fields to make suggestions for future directions.

BackgroundThe study ofde novovariation is important for assessing biological characteristics of new variation and for studies related to human phenotypes. Software programs exist to callde novovariants and programs also exist to test the burden of these variants in genomic regions; however, I am unaware of a program that fits in between these two aspects ofde novovariant assessment. This intermediate space is important for assessing the quality ofde novovariants and to understand the characteristics of the callsets. For this reason, I developed the R package acorn.Resultsacorn is an R package that examines various features ofde novovariants including subsetting the data by individual(s), variant type, or genomic region; calculating features including variant change counts, variant lengths, and presence/absence at CpG sites; and characteristics of parental age in relation tode novovariant counts.Conclusionsacorn is an R package that fills a critical gap in assessingde novovariants and will be of benefit to many investigators studyingde novovariation.

BackgroundThere is increasing evidence of the efficacy of point-of-use water filters on diarrhea prevalence in numerous global settings, in both observational studies and randomized experiments. Most studies, however, are focused on rural locations. Methods We use self-report household surveys to monitor a set of approximately 10,000 households receiving point-of-use water filters and WASH training in Kibera, Kenya. Twenty-five drinking water sources throughout the 7 neighborhoods were also selected for testing of E. coli, total coliform, bacterial 16S rRNA community sequencing and metals. Albendazole was provided to all households at distribution as part of the standard filter distribution protocol, with a subset of 2,642 households not receiving Albendazole at distribution, instead receiving it at the second follow-up (approximately 5 weeks after filter distribution).ResultsAfter data cleaning, a sample of 6,795 households were analyzed using mixed effects generalized linear models to account for repeated household measurements, geospatial and temporal effects, interviewer and other household covariates. Models predicted self-reported, 2-week prevalence of diarrhea. After accounting for confounding factors, self-reported diarrhea rates dropped from 52.7% to 2.2% after approximately 70 days of filter use. Field testing characterized most water sources (18 out of 25) as unsafe for Total coliforms, many for E. coli (6 out of 25), and one source above WHO health guidelines for arsenic. There was no evidence of a difference in self-reported diarrhea prevalence between households receiving Albendazole at distribution vs. those that didn’t (p>0.05).ConclusionsThe introduction of Sawyer filters to households in a densely populated informal settlement reduced diarrhea and other health related problems. Representative water quality testing indicates a high frequency of drinking water source contamination with E. coli and Total Coliforms but a very low frequency of dissolved metals present, above WHO guidelines for drinking water. Anti-parasitic medication distribution had little to no impact on the results. Future randomized controlled studies with objective health measures are needed to ensure cause-effect impact of the filters, and study of filter longevity in the field continues to be a critical need.

Exploring the lived experiences of surgeons is necessary to understand the changing culture of surgery and the unique challenges of being a woman in surgery. Surgeons have significant experiences and observations best discovered through qualitative study. The purpose of this study is to identify the similarities and differences between the experiences of men and women surgeons after initiation of mandatory microaggression training.Qualitative semi-structured interviews with female and male surgeons and residents were done following a year-long series of training sessions on the detrimental effects of microaggression. Participants were selected using a convenience sampling method. MAXQDA coding software (Verbi) was used to evaluate interview transcripts with thematic analysis.Nineteen surgeons and surgical residents were interviewed. The participants were of equal gender identification, with the majority being attending surgeons. Multiple themes highlighted similarities and differences between male and female participants. Differences were noted in identification of a sensitive personality, family planning considerations, and experiences of bias. Similarities were related to the personality traits required to be successful in surgery, the sacrifice inherent to a surgical career, and the war rhetoric used to describe the comradery of residency.The challenges and rewards of surgery are similar for women and men, but women have additional stressors, including gender-based bias, microaggression, and family planning. These stressors take up energy, decreasing the mental space available for additional roles and affecting the work environment. Microaggression education can incite necessary discussions of bias and provide women with an opportunity to reflect on and share their experiences.

Currently, protein-coding de novo variants and large copy number variants have been identified as important for ~30% of individuals with autism. One approach to identify relevant variation in individuals who lack these types of events is by utilizing newer genomic technologies. In this study, highly accurate PacBio HiFi long-read sequencing was applied to a family with autism, epileptic encephalopathy, cognitive impairment, and mild dysmorphic features (two affected female siblings, unaffected parents, and one unaffected male sibling) with no known clinical variant. From our long-read sequencing data, a de novo missense variant in the KCNC2 gene (encodes Kv3.2) was identified in both affected children. This variant was phased to the paternal chromosome of origin and is likely a germline mosaic. In silico assessment revealed the variant was not in controls, highly conserved, and predicted damaging. This specific missense variant (Val473Ala) has been shown in both an ortholog and paralog of Kv3.2 to accelerate current decay, shift the voltage dependence of activation, and prevent the channel from entering a long-lasting open state. Seven additional missense variants have been identified in other individuals with neurodevelopmental disorders (p = 1.03 × 10

Efforts to increase diversity can often be met with resistance amongst high-status groups. Despite this, little is known about majority-group responses towards increasing gender diversity, and the psychological mechanisms underlying them. Across five studies, we extended intergroup threat theory to advance understanding of resistance towards gender diversity amongst men in masculine domains (Studies 1–3 and 5) and amongst women in feminine domains (Study 4). Experimental evidence from male STEM students (Study 1) and professionals (Studies 2 and 5) revealed that realistic threats underlie resistance. Experimentally reducing realistic threat ( N = 165) reduced negative reactions. Whereas realistic-threat-based resistance towards increasing gender diversity did not extend to women in female-dominated domains (Study 4, N = 105), there was a tendency for women high in ingroup identity to show a similar pattern to men. We discuss how we advance theory on diversity resistance, and discuss strategies which may effectively reduce resistance.

Assessing and managing pain while evaluating risks associated with substance use and substance use disorders continues to be a challenge faced by health care clinicians. The American Society for Pain Management Nursing and the International Nurses Society on Addictions uphold the principle that all persons with co-occurring pain and substance use or substance use disorders have the right to be treated with dignity and respect, and receive evidence-based, high quality assessment, and management for both conditions. The American Society for Pain Management Nursing and International Nurses Society on Addictions have updated their 2012 position statement on this topic supporting an integrated, holistic, multidimensional approach, which includes nonopioid and nonpharmacological modalities. Opioid use disorder is used as an exemplar for substance use disorders and clinical recommendations are included with expanded attention to risk assessment and mitigation with interventions targeted to minimize the risk for relapse or escalation of substance use. Opioids should not be excluded for anyone when indicated for pain management. A team-based approach is critical, promotes the active involvement of the person with pain and their support systems, and includes pain and addiction specialists whenever possible. Health care systems should establish policies and procedures that facilitate and support the principles and recommendations put forth in this article.

Political apologies have been theorized to play an important role in healing and reconciliation processes in post-conflict settings. Whether they actually fulfil this function, however, remains unclear as the voices and perspectives of victim communities have largely been underrepresented in research. To address this, we examined the role of apologies that were offered for the El Mozote massacre (El Salvador), the Jeju 4.3 massacres (Republic of Korea) and Bloody Sunday (United Kingdom), according to members of these communities and the broader public. Although we anticipated that victim community members should find the apology more valuable and meaningful and should, therefore, be more positive about its role in healing and reconciliation processes, we found that this varies across countries. This variation could be explained by people's trust in the country's institutions. Across the samples, we found that the apology was seen as a relatively important gesture. For the apology to be perceived as impactful, however, it had to be seen as a meaningful (i.e. sincere) gesture. Our findings suggest that apologies have a role to play in the aftermath of human rights violations, but that it is essential to take the broader context into account.

Researchers and practitioners have highlighted the necessity to monitor jump strategy metrics as well as the commonly reported outcome measures during the countermovement (CMJ) and drop jump (DJ) tests. However, there is a risk of confusion for practitioners, given the vast range of metrics that now seem to be on offer via analysis software when collecting data from force platforms. As such, practitioners may benefit from a framework that can help guide metric selection for commonly used jump tests, which is the primary purpose of this article. To contextualise the proposed framework, we have provided two examples for how this could work: one for the CMJ and one for the DJ, noting that these tests are commonly utilized by practitioners during routine testing across a range of sport performance and clinical settings.

CXCR5+CD8 T cells have attracted significant interest within multiple areas of immunology, cancer, and infection. This is in part due to their apparent dual functionality. These cells perform as cytotoxic cells in a variety of infection states including LCMV, HBV, HIV and SIV. However, CXCR5+CD8 T cells also associate with B cells in peripheral organs and function to stimulate B cell proliferation, antibody/B cell receptor class-switch, and antibody production. CXCR5+CD8 T cells are similar to CXCR5+CD4 T follicular helpers in their genetic make-up, B cell interactions, and functionality despite possessing elevated programmed cell death 1 and cytotoxic proteins. Within cancer CXCR5+CD8 T cells have risen as potential prognostic markers for overall survival and are functionally cytotoxic within tumor microenvironments. In inflammatory disease and autoimmunity, CXCR5+CD8 T cells are implicated in disease progression. During viral infection and cancer, CXCR5 expression on CD8 T cells generally is indicative of progenitor memory stem-like exhausted cells, which are more responsive to immune checkpoint blockade therapy. The use of immune checkpoint inhibitors to overcome immune exhaustion in cancer, and subsequent consequence of immune adverse events, highlights the dual nature of the cellular immune response. This review will detail the functionality of CXCR5+CD8 T cells in cancer and autoimmunity with potential repercussions during immune checkpoint blockade therapy discussed.

Male sex is a strong risk factor for autism spectrum disorder (ASD). The leading theory for a "female protective effect" (FPE) envisions males and females have "differing thresholds" under a "liability threshold model" (DT-LTM). Specifically, this model posits that females require either a greater number or larger magnitude of risk factors (i.e., greater liability) to manifest ASD, which is supported by the finding that a greater proportion of females with ASD have highly penetrant genetic mutations. Herein, we derive testable hypotheses from the DT-LTM for ASD, investigating heritability, familial recurrence, correlation between ASD penetrance and sex ratio, population traits, clinical features, the stability of the sex ratio across diagnostic changes, and highlight other key prerequisites. Our findings reveal that several key predictions of the DT-LTM are not supported by current data, requiring us to establish a different conceptual framework for evaluating alternate models that explain sex differences in ASD.

CXCR5

The American Society for Pain Management Nursing and the International Nurses Society on Addictions hold the position that persons with co-occurring pain and substance use disorder have the right to be treated with dignity and respect, and receive evidence-based, high-quality assessment and management for both conditions using an integrated, holistic, multidimensional approach. Non-opioid and nonpharmacological approaches to pain management are recommended. Opioids should not be withheld from anyone if necessary to treat pain, and a team-based approach, including pain and addiction specialists, should be utilized when possible. Pain management should include interventions aimed at minimizing the risk for relapse or escalation of problematic substance use, and actively involve the person and their support persons in the plan of care. Institutions should establish policies and procedures that support this position statement.

In the eastern Pacific Ocean, hawksbill sea turtles (Eretmochelys imbricata) are adapted to use coastal habitats and ecosystems uncharacteristic of most other sea turtles. Once considered extirpated from this region, hawksbills had sought refuge in estuaries, nesting on muddy banks among the tangles of mangrove roots. This population is at high risk of bycatch during fishing efforts in the estuaries (blast fishing) and adjacent coastal rocky reefs (gillnets), and is further impacted by habitat degradation from coastal development and climate change. The conservation and population recovery of hawksbills in this region is highly dependent on management actions (e.g., nest relocation, habitat protection, bycatch mitigation), and a better understanding of how hawksbills use and move between distinct habitats will help prioritize conservation efforts. To identify multi-year habitat use and movement patterns, we used stable carbon (δ13C) and nitrogen (δ15N) isotope analysis of skin and bone growth layers to recreate movements between two isotopically distinct habitats, a nearshore rocky reef and a mangrove estuary, the latter distinguishable by low δ13C and δ15N values characteristic of a mangrove-based foodweb. We applied skeletochronology with sequential δ13C and δ15N analysis of annual growth layers, “skeleto+iso,” to a dataset of 70 hawksbill humeri collected from coastal El Salvador. The results revealed at least two unique habitat-use patterns. All turtles, regardless of stranding location, spent time outside of the mangrove estuaries during their early juvenile years (< 35 cm curved carapace length, CCL, age 0–5), showing that an oceanic juvenile stage is likely for this population. Juveniles ca. > 35 cm then began to recruit to nearshore areas, but showed divergent habitat-use as some of turtles occupied the coastal rocky reefs, while others settled into the mangrove estuaries. For turtles recruiting to the estuaries, settlement age and size ranged from 3 to 13 years and 35–65 cm CCL. For the adult turtles, age-at-sexual-maturity ranged from 16 to 26 years, and the maximum reproductive longevity observed was 33 years. The skeleto+iso also showed that adult hawksbills have long-term habitat fidelity, and the results demonstrate the importance of both mangrove estuary and nearshore rocky reefs to the conservation of hawksbills in the eastern Pacific.

Links between parental personality, parenting, and adolescent behavior have been well established. However, extant research is limited by the sole focus on parental Big Five personality, and not taking home and family context into account. These gaps were addressed in two studies. In study 1, context, parental personality, and their interactions were examined as predictors of parenting in separate mother and father models (parents only). In study 2, context, parental personality, and parenting were examined as predictors of adolescent behavioral outcomes (parent–adolescent dyads). Parents (N = 283, 45.6% mothers, Mage = 45.51 years) completed assessments of socioeconomic status (SES), adverse childhood experiences (ACEs), personality (Big Five, Dark Triad), and parenting. Adolescents (N = 257, 51.4% female, Mage = 13.65 years) completed an assessment of behavior. Parent Dark Triad domains explained more variance in parental warmth and hostility than the Big Five, but equivalent variance in adolescent behavior. SES interacted with maternal personality, whereas ACEs interacted with paternal personality, to predict parenting behavior. The results showcase the importance of assessing a wider spectrum of parental personality, and examining contextual factors, in affecting adolescent development.

Detection of de novo variants (DNVs) is critical for studies of disease-related variation and mutation rates. To accelerate DNV calling, we developed a graphics processing units-based workflow. We applied our workflow to whole-genome sequencing data from three parent-child sequenced cohorts including the Simons Simplex Collection (SSC), Simons Foundation Powering Autism Research (SPARK), and the 1000 Genomes Project (1000G) that were sequenced using DNA from blood, saliva, and lymphoblastoid cell lines (LCLs), respectively. The SSC and SPARK DNV callsets were within expectations for number of DNVs, percent at CpG sites, phasing to the paternal chromosome of origin, and average allele balance. However, the 1000G DNV callset was not within expectations and contained excessive DNVs that are likely cell line artifacts. Mutation signature analysis revealed 30% of 1000G DNV signatures matched B-cell lymphoma. Furthermore, we found variants in DNA repair genes and at Clinvar pathogenic or likely-pathogenic sites and significant excess of protein-coding DNVs in IGLL5; a gene known to be involved in B-cell lymphomas. Our study provides a new rapid DNV caller for the field and elucidates important implications of using sequencing data from LCLs for reference building and disease-related projects.

Genomic assemblies of the unicellular green alga Chlamydomonas reinhardtii have provided important resources for researchers. However, assembly errors, large gaps, and unplaced scaffolds as well as strain-specific variants currently impede many types of analysis. By combining PacBio HiFi and Oxford Nanopore long-read technologies, we generated a de novo genome assembly for strain CC-5816, derived from crosses of strains CC-125 and CC-124. Multiple methods of evaluating genome completeness and base-pair error rate suggest that the final telomere-to-telomere assembly is highly accurate. The CC-5816 assembly enabled previously difficult analyses that include characterization of the 17 centromeres, rDNA arrays on three chromosomes, and 56 insertions of organellar DNA into the nuclear genome. Using Nanopore sequencing, we identified sites of cytosine (CpG) methylation, which are enriched at centromeres. We analyzed CRISPR-Cas9 insertional mutants in the PF23 gene. Two of the three alleles produced progeny that displayed patterns of meiotic inviability that suggested the presence of a chromosomal aberration. Mapping Nanopore reads from pf23-2 and pf23-3 onto the CC-5816 genome showed that these two strains each carry a translocation that was initiated at the PF23 gene locus on chromosome 11 and joined with chromosomes 5 or 3, respectively. The translocations were verified by demonstrating linkage between loci on the two translocated chromosomes in meiotic progeny. The three pf23 alleles display the expected short-cilia phenotype, and immunoblotting showed that pf23-2 lacks the PF23 protein. Our CC-5816 genome assembly will undoubtedly provide an important tool for the Chlamydomonas research community.

Bishop, C, Abbott, W, Brashill, C, Turner, A, Lake, J, and Read, P. Bilateral vs. unilateral countermovement jumps: comparing the magnitude and direction of asymmetry in elite academy soccer players. J Strength Cond Res 36(6): 1660-1666, 2022-The aims of this study were to compare the magnitude and direction of asymmetry in comparable bilateral and unilateral countermovement jumps (CMJs). Forty-five elite academy soccer players from under-23 (n = 15), under-18 (n = 16), and under-16 (n = 14) age groups performed bilateral and unilateral CMJs as part of their routine preseason fitness testing. For the magnitude of asymmetry, no significant differences were evident for any metric between tests. However, the eccentric impulse asymmetry was significantly greater than mean force and concentric impulse in both bilateral and unilateral tests (p0.01). For the direction of asymmetry, Kappa coefficients showed poor levels of agreement between test measures for all metrics (mean force = -0.15, concentric impulse = -0.07, and eccentric impulse = -0.13). The mean jump data were also presented relative to the body mass for each group. For the bilateral CMJ, significant differences were evident between groups but showed little consistency in the same group performing better or worse across metrics. For the unilateral CMJ, eccentric impulse was the only metric to show meaningful differences between groups, with the under-18 group performing significantly worse than under-23 and under-16 players. This study highlights that despite the magnitude of asymmetry being similar for each metric between comparable bilateral and unilateral CMJs, consistency in the direction of asymmetry was poor. In essence, if the right limb produced the larger force or impulse during a bilateral CMJ, it was rare for the same limb to perform superior during the unilateral task. Thus, practitioners should be aware that bilateral and unilateral CMJs present different limb dominance characteristics and should not use 1 test to represent the other when measuring between-limb asymmetries.

Bishop, C, Read, P, Chavda, S, Jarvis, P, Brazier, J, Bromley, T, and Turner, A. Magnitude or direction? Seasonal variation of interlimb asymmetry in elite academy soccer players. J Strength Cond Res 36(4): 1031-1037, 2022-Previous research has highlighted a distinct lack of longitudinal data for asymmetry. The aims of this study were to provide seasonal variation data for the magnitude and direction of asymmetry. Eighteen elite male academy soccer players (under-23) performed unilateral countermovement jumps (CMJs) and unilateral drop jumps (DJs) during pre-season, mid-season, and end of season time points. Recorded metrics for asymmetry included jump height and concentric impulse for the CMJ, and jump height and reactive strength index for the DJ. The magnitude of asymmetry showed trivial to small changes throughout the season (CMJ effect size [ES] range = -0.43 to 0.05; DJ ES range = -0.18 to 0.41). However, Kappa coefficients showed poor to substantial levels of agreement for the direction of asymmetry during the CMJ (Kappa = -0.06 to 0.77) and DJ (Kappa = -0.10 to 0.78) throughout the season. These data show that when monitoring asymmetry, the magnitude alone may provide a false impression of consistent scores over time. By contrast, monitoring the direction of asymmetry highlights its task and variable nature and is suggested as a useful tool for practitioners who wish to monitor asymmetry over the course of a competitive soccer season.

Genomic regions subject to purifying selection are more likely to carry disease-causing mutations than regions not under selection. Cross species conservation is often used to identify such regions but with limited resolution to detect selection on short evolutionary timescales such as that occurring in only one species. In contrast, genetic intolerance looks for depletion of variation relative to expectation within a species, allowing species-specific features to be identified. When estimating the intolerance of noncoding sequence, methods strongly leverage variant frequency distributions. As the expected distributions depend on ancestry, if not properly controlled for, ancestral population source may obfuscate signals of selection. We demonstrate that properly incorporating ancestry in intolerance estimation greatly improved variant classification. We provide a genome-wide intolerance map that is conditional on ancestry and likely to be particularly valuable for variant prioritization.

Sorensen, AM, Chavda, S, Comfort, P, Lake, J, and Turner, AN. Intra- and interday reliability of weightlifting variables and correlation to performance during cleans. J Strength Cond Res 36(11): 3008-3014, 2022-The purpose of this investigation was to examine intra- and interday reliability of kinetic and kinematic variables assessed during the clean, assess their relationship to clean performance, and determine their suitability in weightlifting performance analysis. Eight competitive weightlifters performed 3 sets of single repetition cleans with 90% of their 1-repetition maximum (1RM). Force-time data were collected via dual force plates with displacement-time data collected via 3-dimensional motion capture, on 3 separate occasions under the same testing conditions. Seventy kinetic and kinematic variables were analyzed for intra- and interday reliability using intraclass correlation coefficients (ICCs) and the coefficient of variation (CV). Pearson's correlation coefficients were calculated to determine relationships between barbell and body kinematics and ground reaction forces, and for correlations to be deemed as statistically significant, an alpha-level of p ≤ 0.005 was set. Eleven variables were found to have "good" to "excellent" intra- and interday ICC (0.779-0.994 and 0.974-0.996, respectively) and CV (0.64-6.89% and 1.14-6.37%, respectively), with strong correlations ( r = 0.880-0.988) to cleans performed at 90% 1RM. Average resultant force of the weighting 1 (W1) phase demonstrated the best intra- and interday reliability (ICC = 0.994 and 0.996, respectively) and very strong correlation ( r = 0.981) to clean performance. Average bar power from point of lift off to peak bar height exhibited the highest correlation ( r = 0.988) to clean performance. Additional reliable variables with strong correlations to clean performance were found, many of these occurred during or included the W1 phase, which suggests that coaches should pay particular attention to the performance of the W1 phase.