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Introduction: A strong link between voice-hearing experience and childhood trauma has been established. The aim of this study was to identify whether there were unique clusters of childhood trauma subtypes in a sample across the clinical spectrum of auditory verbal hallucinations (AVH) and to examine clinical and phenomenological features across these clusters. Methods: Combining two independent international datasets (the Netherlands and Australia), childhood trauma subtypes were examined using hierarchical cluster analysis. Clinical and phenomenological characteristics were compared across emerging clusters using MANOVA and chi-squared analyses. Results: The total sample (n = 413) included 166 clinical individuals with a psychotic disorder and AVH, 122 non-clinical individuals with AVH and 125 non-clinical individuals without AVH. Three clusters emerged: (1) low trauma (n = 299); (2) emotion-focused trauma (n = 71); (3) multi-trauma (n = 43). The three clusters differed significantly on their AVH ratings of amount of negative content, with trend-level effects for loudness, degree of negative content and degree of experienced distress. Furthermore, perceptions of voices being malevolent, benevolent and resistance towards voices differed significantly. Conclusion: The data revealed different types of childhood trauma had different relationships between clinical and phenomenological features of voice-hearing experiences. Thus, implicating different mechanistic pathways and a need for tailored treatment approaches. [ABSTRACT FROM AUTHOR]

Dynamics of the North Sulawesi food security system is studied in this research, where various factors related to food consumption and production are identified. Since rice is accepted as the main food commodity for the Indonesian population, the source of 50% of the average Indonesian’s daily calorie intake, therefore, the behavior of the food security system studied is related to the condition of the availability of rice to meet the consumption of the population in North Sulawesi Province. System dynamics is used as the methodology with the objectives to identify the various factors that influence rice consumption and production in North Sulawesi Province, and develop model accordingly to simulate the dinamics of rice availability in response to changes in rice consumption and production factors. The simulation carried out for a period of 25 years, namely from 2022 to 2047, resulted that at the rice production side, the current productivity cannot meet the need of North Sulawesi Province population consumption, causing importing rice must be carried out to fill up the gap. By improving the production and consumption aspects, such as using high yield variety of rice and introducing food diversification, the availability of rice become sufficient to meet the consumption needs of the people of North Sulawesi. This study then recommends the improvement for sustainable development of food security system in North Sulawesi Province.

Infectious bronchitis virus (IBV) strains of genotype GVIII have been emerging in Europe in the last decade, but no biological characterization has been reported so far. This paper reports the extensive genetic and biological characterization of IBV strain D2860 of genotype GVIII which was isolated from a Dutch layer flock that showed a drop in egg production. Whole genome sequencing showed that it has a high similarity (95%) to CK/DE/IB80/2016 (commonly known as IB80). Cross-neutralization tests with antigens and serotype-specific antisera of a panel of different non-GVIII genotypes consistently gave less than 2% antigenic cross-relationship with D2860. Five experiments using specified pathogen-free chickens of 0, 4, 29 and 63 weeks of age showed that D2860 was not able to cause clinical signs, drop in egg production, false layers or renal pathology. There was also a distinct lack of ciliostasis at both 5 and 8 days post-inoculation at any age, despite proof of infection by immunohistochemical (IHC) staining, RT-PCR and serology. IHC showed immunostaining between 5 and 8 days post inoculation in epithelial cells of sinuses and conchae, while only a few birds displayed immunostaining in the trachea. In vitro comparison of replication of D2860 and M41 in chicken embryo kidney cells at 37°C and at 41°C indicated that D2860 might have a degree of temperature sensitivity that might cause it to prefer the colder parts of the respiratory tract.

Quasi-two-dimensional (2D) metal halide perovskites (MHPs) are promising photovoltaic (PV) materials because of their impressive optical and optoelectronic properties and improved stability compared to their 3D counterparts. The presence of domains with varying numbers of inorganic layers between the organic spacers ( n -phases), each with different bandgaps, makes the photoinduced carrier dynamics in films of these materials complex and intriguing. Existing interpretations of the ultrafast femto- or picosecond spectroscopy data have been inconsistent, most of them focusing either on exciton/charge transfer from low- n to high- n phases or on hot carrier cooling, but not combined. Here, we present a comprehensive study of the carrier dynamics in the Dion-Jacobson type (PDMA)(MA) ( n -1) Pb n I (3 n +1) (PDMA = 1,4-phenylenedimethylammonium, MA = methylammonium) perovskite, stoichiometrically prepared as ⟨ n ⟩ = 5. Within the film, a coexistence of various n -phases is observed instead of solely the n = 5 phase, resulting in an interesting energy landscape for the motion of excitons and charge carriers. We disentangle hot carrier cooling from exciton transfer between low- n and high- n phases using ultrafast time-resolved photoluminescence and transient absorption spectroscopy. Photophysical modeling by target analysis shows that carrier cooling occurring on a subpicosecond time scale is followed by exciton transfer from low- n into high- n phases in ca. 35 ps when the film is excited by 532 or 490 nm light. Carriers in the high- n phase are much longer lived and decay in a ns time window. Overall, our results provide a comprehensive understanding of the photophysics of this material, which helps to optimize quasi-2D MHP materials for a new generation of PV devices., Competing Interests: The authors declare no competing financial interest., (© 2023 The Authors. Published by American Chemical Society.)

Information on the behavior of chickens hatched in different systems is limited and inconsistent across different studies. Changes in broiler activity can be measured automatically and continuously. The aim of this study was to assess the effects of 3 hatching systems on flock activity using a commercial tracking system, and to compare these findings to individual activity measured under experimental conditions. As this experiment was part of a larger study, it was possible to investigate the effects of vaccination on individual activity. In study 1, flock activity was measured in chickens that hatched either conventionally in the hatchery (HH), in a system which provided nutrition in the hatcher (HF), or on-farm (OH). Chickens were reared in 2 batches, in 12 pens/batch (1,155 animals/pen). One camera recorded top-view images of each pen. A daily activity index (moved pixels/total pixels × 100) was calculated by automated image analysis. In study 2, individual activity was measured under experimental conditions using an ultra-wideband (UWB) system. Chickens from the 3 hatching systems were reared in 3 pens (1 pen/treatment, 30 animals/pen). At d14, UWB-tags were attached to 5 chickens/pen, which tracked the distances moved (DM). In study 1, group level activity showed a significant age × hatching system interaction (F 8,752 = 5.83, P < 0.001). HH and HF chickens showed higher activity levels than OH chickens in wk 1, 4, and 5. In wk 3, higher activity levels were measured in HH compared to HF, and in HF compared to OH pens. In contrast, HH chickens in small groups in study 2 showed lower DM than HF and OH chickens in wk 3 (P < 0.001). DM did not differ between treatments before vaccination, however, thereafter, HH chickens showed longer DM, whereas HF and OH chickens moved less. The results indicate that hatching system affected broiler activity at specific ages. Effects found at flock level could not be reproduced by individual measurements in study 2, although stocking density was comparable., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

In 2 experiments, interactions between trace mineral (Zn, Mn, Cu, Se) source (organic or inorganic) in the broiler breeder diet and egg translucency (high or low) on egg characteristics and embryonic development were investigated. In the first experiment, eggs from old breeders (55-57 wk) and in the second experiment, eggs from prime breeders (34-36 wk) were used. Egg composition and bacterial load on the eggshell were analyzed in fresh eggs. During incubation, metabolic heat production of the embryos (d 8 (E8) to 19 of incubation) and tibia ossification (E8.5-E14.5) were determined daily. At hatch, chicken quality was assessed, including tibia biophysical characteristic. Egg quality was not affected by breeder trace minerals source or egg translucency in both experiments. In both experiments, an interaction between trace minerals source and translucency score was found for egg weight loss during incubation. In inorganic trace minerals fed breeders, a high egg translucency resulted in a higher egg weight loss than a low egg translucency, whereas this difference was not seen in organic trace minerals fed breeders. Embryonic heat production and tibia ossification were not affected by trace minerals source or egg translucency. Chicken quality showed ambiguous results between experiment 1 and 2 regarding trace minerals source in the breeder diet. In experiment 2, high translucent eggs from organic fed breeders hatched later than eggs from the other three treatment groups and additionally, high egg translucency resulted in lower residual yolk weight and higher heart and liver percentage of YFBM compared to low egg translucency. Tibia biophysical characteristics at hatch were not affected by trace minerals source or egg translucency. It can be concluded that organic trace minerals source in broiler breeder diet affects eggshell conductance, particularly in low translucent eggs, but effects on chicken quality and tibia characteristics appears to be limited., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Alternative hatching systems have been developed for broiler chickens to provide immediately feed and water after hatch and reduce the number or severity of early life stressors. Besides beneficial effects of these alternative hatching systems on chick quality and performance, broiler health and welfare may be positively affected as well. Especially offspring from young broiler breeder flocks may benefit, as they have been shown to be more sensitive to preturbations than offspring from older breeder flocks. This study evaluated effects of hatching systems on chick quality, health and welfare of young breeder flock offspring, using 3 different hatching systems: conventional hatchery-hatched (HH), hatchery-fed (HF), and on-farm hatching (OH). A total of 24 pens were used in a completely randomized block design, with 8 pens per hatching system and 30 chickens per pen. Chick quality at hatch and performance until 35 d of age was improved in the HF and OH compared to HH treatment, but only minor effects were found on the welfare indicators: footpad dermatitis, hock burn, cleanliness, skin lesion and gait score. No effect was observed on the dynamics of a humoral immune response after NCD vaccination, given at d 0 and 14 of age, as no differences between NCD titers were found at d 18. Animals were vaccinated with a live attenuated infectious bronchitis vaccine virus (IBV) at d 28 to address treatment related differences to disease resilience. The expressions of inflammation and epithelial integrity related genes in the trachea and histo-pathological changes in the trachea were examined at 3 d after vaccine administration. No differences between treatment groups were observed. Although beneficial effects of HF and OH systems were found for young breeder flock offspring on chick quality at hatch and body weight posthatch, only one effect of alternative hatching systems on welfare and health indicators were found. No effect of hatching system on humoral immune response or disease resilience was found., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Genetically encoded visible fluorescent proteins (VFPs) are a key tool used to visualize cellular processes. However, compared to synthetic fluorophores, VFPs are photophysically complex. This photophysical complexity includes the presence of non-emitting, dark proteins within the ensemble of VFPs. Quantitative fluorescence microcopy approaches that rely on VFPs to obtain molecular insights are hampered by the presence of these dark proteins. To account for the presence of dark proteins, it is necessary to know the fraction of dark proteins ( f dark ) in the ensemble. To date, f dark has rarely been quantified, and different methods to determine f dark have not been compared. Here, we use and compare two different methods to determine the f dark of four commonly used VFPs: EGFP, SYFP2, mStrawberry, and mRFP1. In the first, direct method, we make use of VFP tandems and single-molecule two-color coincidence detection (TCCD). The second method relies on comparing the bright state fluorescence quantum yield obtained by photonic manipulation to the ensemble-averaged fluorescence quantum yield of the VFP. Our results show that, although very different in nature, both methods are suitable to obtain f dark . Both methods show that all four VFPs contain a considerable fraction of dark proteins. We determine f dark values between 30 and 60% for the different VFPs. The high values for f dark of these commonly used VFPs highlight that f dark has to be accounted for in quantitative microscopy and spectroscopy.

Improving leg health will support broiler health and welfare. Known factors to improve leg health are: replacing inorganic by organic macro minerals in the diet, providing environmental enrichments and using slower-growing broilers. However, it remains unknown how fast- and slower-growing broilers respond to a combination of providing organic macro minerals and an elevated platform as enrichment with regard to leg health. Therefore, the aim of this study was to identify whether a combined treatment of organic macro minerals and a platform affected leg health, tibia characteristics, behavior and performance of fast- and slower-growing broilers in a semicommercial setting. The experiment had a 2 × 2 factorial arrangement, with 12.800 fast-growing (Ross 308) and 12.800 slower-growing (Hubbard JA757) broilers that were randomly allocated to a control (i.e., inorganic macro minerals without enrichment) or adapted treatment (i.e., organic macro minerals and a platform). Broilers were housed in groups of 800 per pen (47.5 m 2 ), with 8 replicates per treatment (total of 32 pens). Performance was measured weekly and over the total rearing period. Behavior was observed via scan sampling at a target weight of 0.6 and 1.9 kg for both breeds. Walking ability (gait score), footpad dermatitis, and hock burn were assessed in 10 broilers per pen just prior to slaughter weight. Leg disorders and tibia characteristics were assessed in the same broilers at slaughter weight (2.3 kg). Hardly any interaction effects between breed and treatment were found on leg health, tibia characteristics, behavior or performance, suggesting fast- and slower-growing broilers responded to the treatment similarly. The adapted treatment improved tibia characteristics, and increased locomotion and performance, but did not affect leg disorders, walking ability or contact dermatitis in both fast- and slower-growing broilers. The positive effects of the adapted treatment on tibia characteristics in both fast- and slower-growing broilers may improve leg health, although the current study did not confirm this for leg disorders, walking ability or contact dermatitis., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)

Monitoring the performance of polymer-functionalized surfaces that aim at removing and inactivating viruses is typically labor-intensive and time-consuming. This hampers the development and optimization of such surfaces. Here we present experiments of low complexity that can be used to characterize and quantify the antiviral properties of polymer-functionalized surfaces. We showcase our approach on polyethylenimine (PEI)-coated poly(ether sulfone) (PES) microfiltration membranes. We use a fluorescently labeled model virus to quantify both virus removal and inactivation. We directly quantify the log removal of intact viruses by this membrane using single particle counting. Additionally, we exploit the change in photophysical properties upon disassembly of the virus to show that viruses are inactivated by the PEI coating. Although only a small fraction of intact viruses can pass the membrane, a considerable fraction of inactivated, disassembled viruses are found in the filtrate. Fluorescence microscopy experiments show that most of the viruses left behind on the microfiltration membrane are in the inactivated, disassembled state. Combined, our fluorescence microscopy and spectroscopy experiments show that not only does the model virus adsorb to the PEI coating on the membrane but also the interaction with PEI results in the disassembly of the virus capsid., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)