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Background and aims: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by language impairment, and challenges with social interaction, communication, and repetitive behaviors. Although genetics are a primary cause of ASD, the exact genes and molecular mechanisms involved in its pathogenesis are not completely clear. The FOXP2 gene encodes a transcription factor that is known for its major role in language development and severe speech problems. The present study aimed to evaluate the role of FOXP2 in ASD etiology, executive functions, and brain activities. Methods: In the present study, we recruited 450 children with ASD and 490 neurotypical control children. Three domains of executive functions (working memory, response inhibition, and vigilance) were assessed. In addition, five-minute eyes closed electroencephalography was obtained from some of the children with ASD and neurotypical children. DNA sequence and expression level of FOXP2 in blood samples of children with ASD and the control group were evaluated by using sequencing and Real-time PCR, respectively. Results: The results showed no mutations but a significant down expression of FOXP2 genes in children with ASD vs. neurotypical children. Several cognitive and executive function deficiencies were detected in children with ASD. Low alpha and gamma bands in the frontal lobe and high theta bands in the occipital lobe were revealed in children with ASD. We also found several correlations between FOXP2 expression levels and clinical assessments. Conclusions: Our finding revealed the down expression of FOXP2, which could be considered as a biomarker for ASD as well as cognitive and executive dysfunction. Based on brain mapping data, FOXP2 may be related to the theta wave abnormality of children with ASD. FOXP2 may be considered a target of novel treatment to improve memory and executive functions. Implications: Our findings highlight the role of FOXP2 mRNA level in ASD etiology, executive functions, and brain wave frequencies. [ABSTRACT FROM AUTHOR]

Context: Broiler chickens are exposed to various stressors, which can affect production performance. The use of dietary probiotics is one of the feasible methods to maintain performance, intestinal health, humeral immunity, and ameliorating physiological stress in the broiler. Aims: In all, 288 1-day-old male broiler chicks were used to evaluate the effect of probiotic supplementation on performance, morphology, and gene expression associated with immune responses in the starter phase of broiler chickens exposed to stress. Methods: In total, 288 1-day-old male broiler chicks (Ross 308) were assigned to evaluate the effects of Bacillus subtilis spore (BSS; Gallipro®200) supplementation at three levels (0, 0.8 × 105 colony-forming units (CFU) and 1.6 × 105 CFU/g feed) with corticosterone (CORT; 4 mg/kg BW at 7–9 days of age) or without CORT (oil) subcutaneous injection. The experiment was conducted in a completely randomised 2 × 3 factorial design. Key results: CORT injection and probiotic supplementation led to an increase and decrease respectively, in food conversion ratio compared with the control group (P < 0.05). CORT injections reduced the expression of TLR4, HSP70 and IgA in all parts of the intestine. However, the TLR4 gene expression increased in jejunum by CORT injections. BSS supplementation induced the expression of TLR4 in all sections of the intestine, both in stress and non-stress conditions. In stress-exposed chickens, IgA expression in the jejunum was affected by BSS supplementation. Interaction effects between CORT injections and BSS supplementation on the gene expression were significant in different parts of the intestine. Conclusions: Chicks exposed to stress conditions induced by CORT injection showed that the ileum was more susceptible than were the other parts, especially for TLR4 and HSP70 gene expression. BSS-supplemented birds showed more capability to face the stress condition, probably due to intestinal health and immunity-modulated effect of BSS. Implication: Formulating a ration with a probiotic can be more effective in stress conditions, through modulating immune-responsive genes in the intestine. The effects of various environmental stresses on the behavioral, physiological, and performance responses of birds have been studied for years. It has been shown that probiotics, also known direct-feed microbes, substantially influence nutrient digestibility, gut microflora and immunity, and inhibit pathogens and, ultimately, improve growth performance in broilers. Our findings suggested that formulating a ration with probiotic can be more effective in stress condition, with immune response genes modulating the effect in the intestine of broiler chicken. [ABSTRACT FROM AUTHOR]