45 results on '"Minghui HE"'
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2. Taxonomic and phylogenetic characterisations of six species of Pleosporales (in Didymosphaeriaceae, Roussoellaceae and Nigrogranaceae) from China
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Hongmin Hu, Minghui He, Youpeng Wu, Sihan Long, Xu Zhang, Lili Liu, Xiangchun Shen, Nalin N. Wijayawardene, Zebin Meng, Qingde Long, Jichuan Kang, and Qirui Li
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Botany ,QK1-989 - Abstract
Pleosporales comprise a diverse group of fungi with a global distribution and significant ecological importance. A survey on Pleosporales (in Didymosphaeriaceae, Roussoellaceae and Nigrogranaceae) in Guizhou Province, China, was conducted. Specimens were identified, based on morphological characteristics and phylogenetic analyses using a dataset composed of ITS, LSU, SSU, tef1 and rpb2 loci. Maximum Likelihood (ML) and Bayesian analyses were performed. As a result, three new species (Neokalmusia karka, Nigrograna schinifolium and N. trachycarpus) have been discovered, along with two new records for China (Roussoella neopustulans and R. doimaesalongensis) and a known species (Roussoella pseudohysterioides). Morphologically similar species and phylogenetically close taxa are compared and discussed. This study provides detailed information and descriptions of all newly-identified taxa.
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- 2023
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3. Advances in Research on Bacterial Oxidation of Mn(II): A Visualized Bibliometric Analysis Based on CiteSpace
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Wentao Mo, Hang Wang, Jianghan Wang, Yue Wang, Yunfei Liu, Yi Luo, Minghui He, Shuang Cheng, Huiting Mei, Jin He, and Jianmei Su
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Mn(II) oxidation ,manganese oxides ,Mn(II)-oxidizing bacteria ,CiteSpace ,bibliometric analysis ,visualized knowledge map ,Biology (General) ,QH301-705.5 - Abstract
Manganese (Mn) pollution poses a serious threat to the health of animals, plants, and humans. The microbial-mediated Mn(II) removal method has received widespread attention because of its rapid growth, high efficiency, and economy. Mn(II)-oxidizing bacteria can oxidize toxic soluble Mn(II) into non-toxic Mn(III/IV) oxides, which can further participate in the transformation of other heavy metals and organic pollutants, playing a crucial role in environmental remediation. This study aims to conduct a bibliometric analysis of research papers on bacterial Mn(II) oxidation using CiteSpace, and to explore the research hotspots and developmental trends within this field between 2008 and 2023. A series of visualized knowledge map analyses were conducted with 469 screened SCI research papers regarding annual publication quantity, author groups and their countries and regions, journal categories, publishing institutions, and keywords. China, the USA, and Japan published the most significant number of research papers on the research of bacterial Mn(II) oxidation. Research hotspots of bacterial Mn(II) oxidation mainly focused on the species and distributions of Mn(II)-oxidizing bacteria, the influencing factors of Mn(II) oxidation, the mechanisms of Mn(II) oxidation, and their applications in environment. This bibliometric analysis provides a comprehensive visualized knowledge map to quickly understand the current advancements, research hotspots, and academic frontiers in bacterial Mn(II) oxidation.
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- 2024
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4. Decoding the metabolomic responses of Caragana tibetica to livestock grazing in fragile ecosystems
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Minghui He, Yanlong Han, Yong Gao, Min Han, and Liqing Duan
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Caragana tibetica ,grazing intensity ,non-volatile metabolites ,regeneration ,stress response ,Plant culture ,SB1-1110 - Abstract
The population of Caragana tibetica, situated on the edge of the typical grassland-to-desert transition in the Mu Us Sandy Land, plays a vital ecological role in maintaining stability within the regional fragile ecosystem. Despite the consistent growth of C. tibetica following animal grazing, the biological mechanisms underlying its compensatory growth in response to livestock consumption remain unclear. Analyzing 48 metabolomic profiles from C. tibetica, our study reveals that the grazing process induces significant changes in the metabolic pathways of C. tibetica branches. Differential metabolites show correlations with soluble protein content, catalase, peroxidase, superoxide dismutase, malondialdehyde, and proline levels. Moreover, machine learning models built on these differential metabolites accurately predict the intensity of C. tibetica grazing (with an accuracy of 83.3%). The content of various metabolites, indicative of plant stress responses, including Enterolactone, Narceine, and Folcepri, exhibits significant variations in response to varying grazing intensities (P
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- 2024
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5. PtoMYB031, the R2R3 MYB transcription factor involved in secondary cell wall biosynthesis in poplar
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Feng Tang, Bo Jiao, Meng Zhang, Minghui He, Ruiying Su, Keming Luo, and Ting Lan
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secondary cell wall biosynthesis ,R2R3-MYB transcription factor ,PtoMYB031 ,transcriptional inhibition complex ,poplar ,Plant culture ,SB1-1110 - Abstract
IntroductionThe biosynthesis of the secondary cell wall (SCW) is orchestrated by an intricate hierarchical transcriptional regulatory network. This network is initiated by first-layer master switches, SCW-NAC transcription factors, which in turn activate the second-layer master switches MYBs. These switches play a crucial role in regulating xylem specification and differentiation during SCW formation. However, the roles of most MYBs in woody plants are yet to be fully understood.MethodsIn this study, we identified and isolated the R2R3-MYB transcription factor, PtoMYB031, from Populus tomentosa. We explored its expression, mainly in xylem tissues, and its role as a transcriptional repressor in the nucleus. We used overexpression and RNA interference techniques in poplar, along with Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays, to analyze the regulatory effects of PtoMYB031.ResultsOverexpression of PtoMYB031 in poplar significantly reduced lignin, cellulose, and hemicellulose content, and inhibited vascular development in stems, resulting in decreased SCW thickness in xylem tissues. Gene expression analysis showed that structural genes involved in SCW biosynthesis were downregulated in PtoMYB031-OE lines. Conversely, RNA interference of PtoMYB031 increased these compounds. Additionally, PtoMYB031 was found to recruit the repressor PtoZAT11, forming a transcriptional inhibition complex.DiscussionOur findings provide new insights into how PtoMYB031, through its interaction with PtoZAT11, forms a complex that can suppress the expression of key regulatory genes, PtoWND1A and PtoWND2B, in SCW biosynthesis. This study enhances our understanding of the transcriptional regulation involved in SCW formation in poplar, highlighting the significant role of PtoMYB031.
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- 2024
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6. Analysis of Lake Area Dynamics and Driving Forces in the Jianghan Plain Based on GEE and SEM for the Period 1990 to 2020
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Minghui He and Yi Liu
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Jianghan Plain ,Google Earth Engine ,water body aera extraction ,PLS-SEM ,driving force analysis ,Science - Abstract
The lakes of Jianghan Plain, as an important component of the water bodies in the middle and lower reaches of the Yangtze River plain, have made significant contributions to maintaining the ecological health and promoting the sustainable development of the Jianghan Plain. However, there is a relatively limited understanding regarding the trends of lake area change for different types of lakes and their dominant factors over the past three decades in the Jianghan Plain. Based on the Google Earth Engine (GEE) platform, combined with the water body index method, the changes in area of three different types of lakes (area > 1 km2) in the Jianghan Lake Group from 1990 to 2020 were extracted and analyzed. Additionally, the Partial least squares structural equation model (PLS-SEM) was utilized to analyze the driving factors affecting the changes in water body area of these lakes. The results show that from 1990 to 2020, the area of the lakes of the wet season and level season exhibited a decreasing trend, decreasing by 893.1 km2 and 77.9 km2, respectively. However, the area of dry season lakes increased by 59.27 km2. The areas of all three types of lakes reached their minimum values in 2006. According to the PLS-SEM results, the continuous changes in the lakes’ area are mainly controlled by environmental factors overall. Furthermore, human factors mainly influence the mutation of the lakes’ area. This study achieved precise extraction of water body areas and accurate analysis of driving factors, providing a basis for a comprehensive understanding of the dynamic changes in the lakes of Jianghan Plain, which is beneficial for the rational utilization and protection of water resources.
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- 2024
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7. Correction: Wu et al. Rust Fungi on Medicinal Plants in Guizhou Province with Descriptions of Three New Species. J. Fungi 2023, 9, 953
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Qianzhen Wu, Minghui He, Tiezhi Liu, Hongmin Hu, Lili Liu, Peng Zhao, and Qirui Li
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n/a ,Biology (General) ,QH301-705.5 - Abstract
Error in Figure [...]
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- 2023
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8. Rust Fungi on Medicinal Plants in Guizhou Province with Descriptions of Three New Species
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Qianzhen Wu, Minghui He, Tiezhi Liu, Hongmin Hu, Lili Liu, Peng Zhao, and Qirui Li
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medicinal plant ,molecular phylogeny ,new taxa ,phytopathogen ,Pucciniales ,Biology (General) ,QH301-705.5 - Abstract
During the research on rust fungi in medicinal plants of Guizhou Province, China, a total of 9 rust fungal species were introduced, including 3 new species (Hamaspora rubi-alceifolii, Nyssopsora altissima, and Phragmidium cymosum), as well as 6 known species (Melampsora laricis-populina, Melampsoridium carpini, Neophysopella ampelopsidis, Nyssopsora koelrezidis, P. rosae-roxburghii, P. tormentillae). Notably, N. ampelopsidis and P. tormentillae were discovered for the first time in China, while M. laricis-populina, Me. carpini, and Ny. koelreuteriae were first documented in Guizhou Province. Morphological observation and molecular phylogenetic analyses of these species with similar taxa were compared to confirm their taxonomic identities, and taxonomic descriptions, illustrations and host species of those rust fungi on medicinal plant are provided.
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- 2023
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9. A smart nanoplatform for enhanced photo-ferrotherapy of hepatocellular carcinoma
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Longguang Tang, Mingjian Ling, Madiha Zahra Syeda, Rui Sun, Minghui He, Qingchun Mu, Xiulong Zhu, Chunming Huang, and Liao Cui
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GSH ,ferrotherapy ,PTT ,hepatocellular carcinoma ,nanoparticle ,Biotechnology ,TP248.13-248.65 - Abstract
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Emerging therapies, such as ferroptosis mediated cancer therapy and phototherapy, offer new opportunities for HCC treatment. The combination of multiple treatments is often more effective than monotherapy, but many of the current treatments are prone to serious side effects, resulting in a serious decline in patients’ quality of life. Therefore, the combination therapy of tumor in situ controllable activation will improve the efficacy and reduce side effects for precise treatment of tumor. Herein, we synthesized a GSH-activatable nanomedicine to synergize photothermal therapy (PTT) and ferrotherapy. We utilized a near-infrared dye SQ890 as both an iron-chelating and a photothermal converter agent, which was encapsulated with a GSH-sensitive polymer (PLGA-SS-mPEG), to attain the biocompatible SQ890@Fe nanoparticles (NPs). In the tumor microenvironment (TME), SQ890@Fe NPs showed a GSH-activated photothermal effect that could increase the Fenton reaction rate. Meanwhile, the depletion of GSH could further increase ferroptosis effect. In turn, the increasing radical generated by ferrotherapy could impair the formation of heat shock proteins (HSPs) which could amplify PTT effects by limiting the self-protection mechanism. Overall, the intelligent nanomedicine SQ890@Fe NPs combines ferrotherapy and PTT to enhance the efficacy and safety of cancer treatment through the mutual promotion of the two treatment mechanisms, providing a new dimension for tumor combination therapy.
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- 2022
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10. FOXO Dictates Initiation of B Cell Development and Myeloid Restriction in Common Lymphoid Progenitors
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Lucía Peña-Pérez, Shabnam Kharazi, Nicolai Frengen, Aleksandra Krstic, Thibault Bouderlique, Julia Hauenstein, Minghui He, Ece Somuncular, Xiaoze Li Wang, Carin Dahlberg, Charlotte Gustafsson, Ann-Sofie Johansson, Julian Walfridsson, Nadir Kadri, Petter Woll, Marcin Kierczak, Hong Qian, Lisa Westerberg, Sidinh Luc, and Robert Månsson
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B cell ,FOXO (forkhead box protein O) ,lineage commitment/specification ,myeloid restriction ,gene regulation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
The development of B cells relies on an intricate network of transcription factors critical for developmental progression and lineage commitment. In the B cell developmental trajectory, a temporal switch from predominant Foxo3 to Foxo1 expression occurs at the CLP stage. Utilizing VAV-iCre mediated conditional deletion, we found that the loss of FOXO3 impaired B cell development from LMPP down to B cell precursors, while the loss of FOXO1 impaired B cell commitment and resulted in a complete developmental block at the CD25 negative proB cell stage. Strikingly, the combined loss of FOXO1 and FOXO3 resulted in the failure to restrict the myeloid potential of CLPs and the complete loss of the B cell lineage. This is underpinned by the failure to enforce the early B-lineage gene regulatory circuitry upon a predominantly pre-established open chromatin landscape. Altogether, this demonstrates that FOXO3 and FOXO1 cooperatively govern early lineage restriction and initiation of B-lineage commitment in CLPs.
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- 2022
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11. Neoadjuvant programmed cell death 1 blockade combined with chemotherapy for resectable esophageal squamous cell carcinoma
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Ning Zhang, Honglei Wang, Fang Wang, Chao Cheng, Shuling Chen, Xiaoyan Wang, Yong Bao, Sui Peng, Yaron Shargall, Fang Peng, Sai-Ching Jim Yeung, Biniam Kidane, Minghui He, Bo Zeng, Shiting Feng, Weixiong Yang, Xiangbin Xing, Siyu Wang, Wenfang Chen, Zhenguo Liu, Christopher W Seder, Kazuo Koyanagi, and Honghe Luo
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Published
- 2022
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12. Dynamic modeling and analysis for a differential modular robot joint with the friction model.
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Yukun Liu, Ruiqing Luo, Minghui He, Liang Du 0002, Sheng Bao, Jianjun Yuan, and Weiwei Wan
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- 2022
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13. A Parameter Identification Method for Coupled Dynamics of Robotic Manipulator with Differential Modular Joints.
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Minghui He, Ruiqing Luo, Yukun Liu, Liang Du 0002, Sheng Bao, Jianjun Yuan, and Weiwei Wan
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- 2022
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14. Research on inertial reference units prescribed performance integral sliding mode angle control
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Ling Zhao, Minghui He, and Xinyue Cao
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Atmospheric Science ,Geophysics ,Space and Planetary Science ,Aerospace Engineering ,General Earth and Planetary Sciences ,Astronomy and Astrophysics - Published
- 2023
15. Self-Healing Multimodal Flexible Optoelectronic Fiber Sensors
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Xiaochun Wang, Guangxue Chen, Kaili Zhang, Ren’ai Li, Zihan Jiang, Hongyou Zhou, Jiulin Gan, and Minghui He
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General Chemical Engineering ,Materials Chemistry ,General Chemistry - Published
- 2023
16. Increased cross-presentation by dendritic cells and enhanced anti-tumour therapy using the Arp2/3 inhibitor CK666
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Mariana M. S. Oliveira, Roberta D’Aulerio, Tracer Yong, Minghui He, Marisa A. P. Baptista, Susanne Nylén, and Lisa S. Westerberg
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Cancer Research ,Oncology - Abstract
Background Dendritic cell (DC) vaccines for cancer therapy offer the possibility to let the patient’s own immune system kill cancer cells. However, DC vaccines have shown less efficacy than expected due to failure to induce cancer cell killing and by activating T regulatory cells. Methods We tested if inhibition of signalling via WASp and Arp2/3 using the small molecule CK666 would enhance DC-mediated killing of tumour cells in vitro and in vivo. Results Using CK666 during the ex vivo phase of antigen processing of ovalbumin (OVA), murine and human DCs showed decreased phagosomal acidification, indicating activation of the cross-presentation pathway. When compared to untreated DCs, DCs treated with CK666 during uptake and processing of OVA-induced increased proliferation of OVA-specific CD8+ OT-I T cells in vitro and in vivo. Using the aggressive B16-mOVA melanoma tumour model, we show that mice injected with CK666-treated DCs and OVA-specific CD8+ OT-I T cells showed higher rejection of B16 melanoma cells when compared to mice receiving non-treated DCs. This resulted in the prolonged survival of tumour-bearing mice receiving CK666-treated DCs. Moreover, combining CK666-treated DCs with the checkpoint inhibitor anti-PD1 further prolonged survival. Conclusion Our data suggest that the small molecule inhibitor CK666 is a good candidate to enhance DC cross-presentation for cancer therapy.
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- 2023
17. Distinct single-cell immune ecosystems distinguish true and de novo HBV-related hepatocellular carcinoma recurrences
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Shuling Chen, Cheng Huang, Guanrui Liao, Huichuan Sun, Yubin Xie, Changyi Liao, Jianping Wang, Minghui He, Huanjing Hu, Zihao Dai, Xiaoxue Ren, Xuezhen Zeng, Zhilong Lin, Guo-Pei Zhang, Wenxuan Xie, Shunli Shen, Shaoqiang Li, Sui Peng, Dong-Ming Kuang, Qiang Zhao, Dan G Duda, and Ming Kuang
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Gastroenterology - Abstract
ObjectiveRevealing the single-cell immune ecosystems in true versus de novo hepatocellular carcinoma (HCC) recurrences could help the optimal development of immunotherapies.DesignWe performed 5’and VDJ single-cell RNA-sequencing on 34 samples from 20 recurrent HCC patients. Bulk RNA-sequencing, flow cytometry, multiplexed immunofluorescence, and in vitro functional analyses were performed on samples from two validation cohorts.ResultsAnalyses of mutational profiles and evolutionary trajectories in paired primary and recurrent HCC samples using whole-exome sequencing identified de novo versus true recurrences, some of which occurred before clinical diagnosis. The tumour immune microenvironment (TIME) of truly recurrent HCCs was characterised by an increased abundance in KLRB1+CD8+T cells with memory phenotype and low cytotoxicity. In contrast, we found an enrichment in cytotoxic and exhausted CD8+T cells in the TIME of de novo recurrent HCCs. Transcriptomic and interaction analyses showed elevated GDF15 expression on HCC cells in proximity to dendritic cells, which may have dampened antigen presentation and inhibited antitumour immunity in truly recurrent lesions. In contrast, myeloid cells’ cross talk with T cells-mediated T cell exhaustion and immunosuppression in the TIME ofde novorecurrent HCCs. Consistent with these findings, a phase 2 trial of neoadjuvant anti-PD-1 immunotherapy showed more responses in de novo recurrent HCC patients.ConclusionTrue and de novo HCC recurrences occur early, have distinct TIME and may require different immunotherapy strategies. Our study provides a source for genomic diagnosis and immune profiling for guiding immunotherapy based on the type of HCC recurrence and the specific TIME.
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- 2023
18. Investigation on the Material Basis of Sijicao Granules in Treating Eczema Based on Network Pharmacology.
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Yitong SHEN, Bo TU, Yaru YANG, Li JIANG, Minghui HE, and Yan LIN
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ECZEMA ,GALLIC acid ,MOLECULAR docking ,CELL anatomy ,CELLULAR signal transduction - Abstract
Objectives] To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical com- position-network pharmacology. [Methods] First of all, the chemical constituents of Polygonum capitatum and Plantago asiatica from Sijicao granules were collected, and the relevant target information of the constituents was collected by TCMSP, PubChem, DisGeNET, GeneCards and STRING databases. Furthermore, Cytoscape 3.8.2 software was used to construct the chemical compounds-target network map of Sijicao granules. Finally, STRING database was used for PPI protein network analysis, GO functional enrichment analysis and KEGG pathway enrich- ment analysis of core targets, and molecular docking between core constituents and protein targets was also performed. [Results] 30 constitu- ents, including quercetin, kaempferol, luteolin, ellagic acid and gallic acid, were discovered to be the key effective compounds of Sijicao gran- ules in the treatment of eczema. And its core action protein targets were PTGS2, NOS2, AKTI, TP53, IL6, HMOX1. What's more, through GO functional enrichment analysis of biological process (BP), cell component (CC), molecular function (MF) analysis and KEGG pathway enrichment analysis, the main pathways of action of Sijicao granules for the treatment of eczema including IL-17 signaling pathway, T cell re- ceptor signaling pathway, cancer signaling pathway, TNF signaling pathway and Relaxin signaling pathway. In addition, molecular docking re- sults displayed that the primary active constituents quercetin, kaempferol and luteolin were well combined with the core protein targets AKTI and IL6. [Conclusions] Sijicao granules could play an important role for the treatment of eczema through multi-component, multi-target, multi-pathway and their interaction. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Supplementary Figure from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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Ming Kuang, Zhenwei Peng, Baogang Peng, Jiaming Lai, Dongming Li, Shunli Shen, Shaoqiang Li, Lixia Xu, Jiehui Tan, Zelong Liu, Zihao Dai, Xiangjun Zhou, Xiaoshuang Li, Yifan Ma, Longqing Tang, Yanyan Han, Minghui He, Huanjing Hu, Yubin Xie, Bin Li, Qian Zhou, Han Xiao, Zebin Chen, Wei Wang, Tianhong Su, Xuezhen Zeng, Jie Mei, Wei Hu, Shuling Chen, and Sui Peng
- Abstract
Supplementary Figure from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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- 2023
20. Data from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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Ming Kuang, Zhenwei Peng, Baogang Peng, Jiaming Lai, Dongming Li, Shunli Shen, Shaoqiang Li, Lixia Xu, Jiehui Tan, Zelong Liu, Zihao Dai, Xiangjun Zhou, Xiaoshuang Li, Yifan Ma, Longqing Tang, Yanyan Han, Minghui He, Huanjing Hu, Yubin Xie, Bin Li, Qian Zhou, Han Xiao, Zebin Chen, Wei Wang, Tianhong Su, Xuezhen Zeng, Jie Mei, Wei Hu, Shuling Chen, and Sui Peng
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A high rate of recurrence after curative therapy is a major challenge for the management of hepatocellular carcinoma (HCC). Currently, no effective adjuvant therapy is available to prevent HCC recurrence. We designed a personalized neoantigen-loaded dendritic cell vaccine and neoantigen-activated T-cell therapy, and used it as adjuvant therapy to treat 10 patients with HCC who had undergone curative resection or radiofrequency ablation in the first stage of a phase II trial (NCT03067493). The primary outcomes were safety and neoantigen-specific immune response. Disease-free survival (DFS) was also evaluated. The immunotherapy was successfully administered to all the patients without unexpected delay and demonstrated a reasonable safety profile with no grade ≥3 treatment-related side effects reported. Seventy percent of patients generated de novo circulating multiclonal neoantigen-specific T-cell responses. Induced neoantigen-specific immunity was maintained over time, and epitope spreading was observed. Patients who generated immune responses to treatment exhibited prolonged DFS compared with nonresponders (P = 0.012), with 71.4% experiencing no relapse for 2 years after curative treatment. High expression of an immune stimulatory signature, enhanced immune-cell infiltration (i.e., CD8+ T cells), and upregulated expression of T-cell inflammatory gene profiles were found in the primary tumors of the responders. In addition, neoantigen depletion (immunoediting) was present in the recurrent tumors compared with the primary tumors (7/9 vs. 1/17, P = 0.014), suggesting that immune evasion occurred under the pressure of immunotherapy. Our study indicates that neoantigen-based combination immunotherapy is feasible, safe, and has the potential to reduce HCC recurrence after curative treatment.
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- 2023
21. A Repeatable Self-Adhesive Liquid-Free Double-Network Ionic Conductor with Tunable Multifunctionality
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Ziyu Hua, Guangxue Chen, Kai Zhao, Ren’ai Li, and Minghui He
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General Materials Science - Abstract
Liquid-free ionic conductors (LFICs) have promising applications in flexible electronics because most ionic conductors currently suffer from ionic liquid leakage or water evaporation issues. However, it has been a formidable challenge for LFICs to achieve long-term repeated self-adhesion on different substrates, especially on soft biological tissues. Based on the double-network design concept, we first fabricate a series of repeatable self-adhesive liquid-free double-network ionic conductors (SALFDNICs), consisting of stretchable first poly(AA-ChCl)-type supramolecular deep eutectic polymer networks and stiff second polydopamine (PDA) networks, which can maintain sufficient dynamic hydrogen bonds and catechol groups in the ionic conductors by preventing the overoxidation of dopamine, thus balancing the contradiction between adhesion and cohesion in liquid-free ionic conductors. Therefore, SALFDNICs can instantly form various interface interaction forces with multiple substrates (adhesion strength up to 757 N/m) and firmly adhere to various substrates for 20 detachment-reattachment cycles with a reduction in adhesion strength of less than 15%. Furthermore, SALFDNICs also have other comprehensive properties, such as optimum self-healing properties (self-healing efficiency of 90%), good stretchability (strain at break of 1200%), and promising conductivity (2.31 × 10
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- 2022
22. Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
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Sui Peng, Shuling Chen, Wei Hu, Jie Mei, Xuezhen Zeng, Tianhong Su, Wei Wang, Zebin Chen, Han Xiao, Qian Zhou, Bin Li, Yubin Xie, Huanjing Hu, Minghui He, Yanyan Han, Longqing Tang, Yifan Ma, Xiaoshuang Li, Xiangjun Zhou, Zihao Dai, Zelong Liu, Jiehui Tan, Lixia Xu, Shaoqiang Li, Shunli Shen, Dongming Li, Jiaming Lai, Baogang Peng, Zhenwei Peng, and Ming Kuang
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Cancer Research ,Carcinoma, Hepatocellular ,Antigens, Neoplasm ,Liver Neoplasms ,Vaccination ,Immunology ,Immunity ,Humans ,Dendritic Cells ,Immunotherapy ,Neoplasm Recurrence, Local ,Cancer Vaccines - Abstract
A high rate of recurrence after curative therapy is a major challenge for the management of hepatocellular carcinoma (HCC). Currently, no effective adjuvant therapy is available to prevent HCC recurrence. We designed a personalized neoantigen-loaded dendritic cell vaccine and neoantigen-activated T-cell therapy, and used it as adjuvant therapy to treat 10 patients with HCC who had undergone curative resection or radiofrequency ablation in the first stage of a phase II trial (NCT03067493). The primary outcomes were safety and neoantigen-specific immune response. Disease-free survival (DFS) was also evaluated. The immunotherapy was successfully administered to all the patients without unexpected delay and demonstrated a reasonable safety profile with no grade ≥3 treatment-related side effects reported. Seventy percent of patients generated de novo circulating multiclonal neoantigen-specific T-cell responses. Induced neoantigen-specific immunity was maintained over time, and epitope spreading was observed. Patients who generated immune responses to treatment exhibited prolonged DFS compared with nonresponders (P = 0.012), with 71.4% experiencing no relapse for 2 years after curative treatment. High expression of an immune stimulatory signature, enhanced immune-cell infiltration (i.e., CD8+ T cells), and upregulated expression of T-cell inflammatory gene profiles were found in the primary tumors of the responders. In addition, neoantigen depletion (immunoediting) was present in the recurrent tumors compared with the primary tumors (7/9 vs. 1/17, P = 0.014), suggesting that immune evasion occurred under the pressure of immunotherapy. Our study indicates that neoantigen-based combination immunotherapy is feasible, safe, and has the potential to reduce HCC recurrence after curative treatment.
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- 2022
23. Self-Adhesive Dry Ionic Conductors Based on Supramolecular Deep Eutectic Polymers
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Kaili Zhang, Ren’ai Li, Guangxue Chen, Xiaochun Wang, and Minghui He
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General Chemical Engineering ,Materials Chemistry ,General Chemistry - Published
- 2022
24. Table S5 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
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Table S5 shows detailed information of structure variations in the six HCC patients with WGS analysis.
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- 2023
25. Figure S1-S17 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Fig.S1 shows flow chart of enrollment for multifocal HCC patients with anti-PD-1 therapy. Fig.S2 shows CT images of the six HCC patients with two tumors in the liver. Fig.S3 shows purity of tumor content of the six HCC patients with two tumors in the liver. Fig.S4 shows HBV integration sites in the six HCC patients with two tumors in the liver. Fig.S5 shows expression level of TERT in the six HCC patients with two tumors in the liver. Fig.S6 shows landscape of inter-chromosomal translocations and copy number alterations in small and large tumors of four cases. Fig.S7 shows number and types of chromosomal rearrangement in the six HCC patients with two tumors in the liver. Fig.S8 shows copy number aberrations in the six HCC patients with two tumors in the liver. Fig.S9 shows mutational landscape of the six HCC patients with two tumors in the liver. Fig.S10 shows non-silent mutations in the six HCC patients with two tumors in the liver. Fig.S11 shows profile of mutational cancer cell fractions of small and large tumors in the six HCC patients. Fig.S12 shows unsupervised clustering of RNA expression in tumors and adjacent normal liver tissues of 12 multifocal HCC patients. Fig.S13 shows expression level of immune functional genes in small and large tumors of 12 multifocal HCC patients. Fig.S14 shows expression level of immune stimulators in small and large tumors of 12 multifocal HCC patients. Fig.S15 shows expression level of immune inhibitors in small and large tumors of 12 multifocal HCC patients. Fig.S16 shows mutations of immune-related genes in the six HCC patients with two tumor nodules in the liver. Fig.S17 shows images of the small and large tumors before and after anti-PD-1 therapy.
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- 2023
26. Data from Multiomic Analysis Reveals Comprehensive Tumor Heterogeneity and Distinct Immune Subtypes in Multifocal Intrahepatic Cholangiocarcinoma
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Sui Peng, Ming Kuang, Jian Ren, Baogang Peng, Jiaming Lai, Dongming Li, Shaoqiang Li, Shunli Shen, Hong Peng, Qianwen Zeng, Xiaoxue Ren, Jianping Wang, Yuanqi Wang, Changyi Liao, Lijuan Liu, Minghui He, Huanjing Hu, Yuhong Cai, Yubin Xie, and Shuling Chen
- Abstract
Purpose:Targeted therapy and immunotherapy are transforming the treatment approach for intrahepatic cholangiocarcinoma (ICC). However, little is known about the intertumor heterogeneity (ITH) of multifocal ICC and its impacts on patient response to these treatments. We aimed to characterize the immunogenomic and epigenomic heterogeneity of multifocal ICC to guide treatment decision making.Experimental Design:We obtained 66 tumor samples from 16 patients with multifocal ICC and characterized the tumor and immune heterogeneity using whole-exome sequencing, bulk and single-cell RNA sequencing, methylation microarray, and multiplex immunostaining. Patients were divided into high- or low-ITH groups according to the median ITH index. Two independent cohorts were used to validate findings. Responses to anti-PD-1 therapy were assessed.Results:Multifocal ICC presented considerable intertumor genomic, transcriptional, and epigenomic heterogeneity within a patient in high ITH group. The immune profile among multiple tumors within a patient was relatively less heterogeneous in high- or low-ITH group, and consistent responses of multiple tumors to anti-PD-1 immunotherapy were observed. Unsupervised clustering of immune markers identified one low and one high immune subtype, with higher immune cell infiltration, closer tumor–immune cell interactions, and upregulated IFN-signature expression in high-immune subtype. Determining expression levels of CD8B and ICOS facilitated this immune classification and prediction of patient prognosis. Finally, promoter DNA methylation contributed to different immune profiles of two subtypes by regulating immune-gene expression.Conclusions:There is comprehensive heterogeneity in the genome, transcriptome, and epigenome of multifocal ICC. On the basis of the less heterogeneous immune profile of ICC, we suggest an immune classification that stratifies patients' prognosis and may support personalized immunotherapy.
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- 2023
27. Supplementary Data from Multiomic Analysis Reveals Comprehensive Tumor Heterogeneity and Distinct Immune Subtypes in Multifocal Intrahepatic Cholangiocarcinoma
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Sui Peng, Ming Kuang, Jian Ren, Baogang Peng, Jiaming Lai, Dongming Li, Shaoqiang Li, Shunli Shen, Hong Peng, Qianwen Zeng, Xiaoxue Ren, Jianping Wang, Yuanqi Wang, Changyi Liao, Lijuan Liu, Minghui He, Huanjing Hu, Yuhong Cai, Yubin Xie, and Shuling Chen
- Abstract
Supplementary Data from Multiomic Analysis Reveals Comprehensive Tumor Heterogeneity and Distinct Immune Subtypes in Multifocal Intrahepatic Cholangiocarcinoma
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- 2023
28. Table S1, Table S2, Table S4, Table S8, Table S10, Table S11 from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
- Author
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Table S1 shows clinicopathologic information of 12 multifocal HCC patients. Table S2 shows PCR primers used for validation of HBV integration sites. Table S4 shows HBV integration analysis in HCC patients with WGS data. Table S8 shows P-value of pathways enrichment analysis in the small and large tumors of 12 multifocal HCC patients. Table S10 shows correlation between the number of rearrangements and immune activity. Table S11 shows detailed clinical data of eight patients with anti-PD-1 therapy.
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- 2023
29. Data from The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas
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Sui Peng, Lixia Xu, Ming Kuang, Jun Yu, Lijian Liang, Baogang Peng, Dongming Li, Lujing Fang, Han Xiao, Xiaoxing Li, Yubin Xie, Shunli Shen, Heping Li, Yu Guo, Minghui He, and Manling Huang
- Abstract
Purpose:Immune checkpoint inhibitor therapy is emerging as the promising option for patients with advanced hepatocellular carcinoma. We aimed to investigate the heterogeneity of different tumor nodules of the same patient with multifocal hepatocellular carcinomas in response to immunotherapy and its molecular mechanisms.Experimental Design:We attained 45 surgical tumor samples including 33 small and 12 large nodules from 12 patients with multifocal hepatocellular carcinoma and evaluated genomic and immune heterogeneity among tumors through whole-genome sequencing and RNA sequencing. IHC was performed to validate the expression of immune markers. The responses to anti–programmed cell death protein-1 (PD-1) therapy in patients with multifocal hepatocellular carcinoma were evaluated.Results:The small and large tumors within the same patient presented with similar genomic characteristics, indicating their same genomic origin. We further found the small tumors had higher immune cell infiltration including more CD8+ T cells, M1 macrophages, and monocytes as compared with large tumors. Besides, the expression of interferon signature predictive of response to anti–PD-1 therapy was significantly upregulated in the small tumors. Moreover, the immune pathways were more vigorous along with less active proliferation pathways in the small tumors. In keeping with this, we found that small nodules were more sensitive to anti–PD-1 therapy than large nodules in patients with multifocal hepatocellular carcinoma.Conclusions:The small tumors in patients with multifocal hepatocellular carcinoma had higher immune cell infiltration and upregulation of immune pathways as compared with the large tumors, which can partially explain the different responses of small and large tumors in the same case to anti–PD-1 therapy.
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- 2023
30. Capitaone A-B, Antioxidant and Cytotoxic Activities Phenolic Compounds from Polygonum Capitatum
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Lei He, Fei Zhong, XingJun Chen, Ya-Ru Yang, XueLong Yan, MingHui He, Xu Zhang, Zhu Zeng, Bo Tu, QingDe Long, and Yan Lin
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- 2023
31. Research Status and Progress of Biomass-Based 3D Printing Materials
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Hanyu Zhao, Ying Jia, Guangxue Chen, Minghui He, Junfei Tian, and Qifeng Chen
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- 2023
32. Superhydrophilic three-dimensional porous spent coffee ground reduced palladium nanoparticles for efficient catalytic reduction
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Shaokai Zhang, Guangxue Chen, Minghui He, Zhaohui Yu, Junfei Tian, Huifang Chan, Xiao-Fang Wan, Congcan Shi, Zhangxiong Wu, and Shenghong Sun
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Materials science ,Water transport ,Metal Nanoparticles ,Selective catalytic reduction ,Hydrogen Peroxide ,Coffee ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Catalysis ,Nanocellulose ,Biomaterials ,Colloid and Surface Chemistry ,Chemical engineering ,Superhydrophilicity ,Water treatment ,Chemical stability ,Porosity ,Palladium - Abstract
The use of functional biodegradable wastes to treat environmental problems would create minimal extra burden to our environment. In this paper, we propose a sustainable and practical strategy to turn spent coffee ground (SCG) into a multifunctional palladium-loaded catalyst for water treatment instead of going into landfill as solid waste. Bleached delignified coffee ground (D-SCG) has a porous structure and a good capability to reduce Pd (II) to Pd (0). A large amount of nanocellulose is formed on the surface of SCG after bleaching by H2O2, which anchors and disperses the palladium nanoparticles (Pd NPs). The D-SCG loaded with Pd NPs (Pd-D-SCG) is superhydrophilic, which facilitates water transport and thus promotes efficient removal of organic pollutants dissolved in water. Pd-D-SCG exhibits excellent room temperature catalytic activity for the removal of 4-nitrophenol (4-NP) and methylene blue (MB) in water and shows good chemical stability and recyclability in water, with no obvious decrease even after five repeated cycles.
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- 2022
33. 3D printed, environment tolerant all-solid-state capacitive ionic skin
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Yixuan Wu, Ling Cai, Guangxue Chen, Fengzhi Yang, and Minghui He
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Renewable Energy, Sustainability and the Environment ,General Materials Science ,General Chemistry - Abstract
By the photopolymerization 3D printing of photopolymerizable deep eutectic solvents, a stable and sensitive solid-state capacitive ionic skin is reported.
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- 2022
34. Systematic Evaluation of Pharmacognostic Identification of Polygonum capitatum.
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Bo TU, Xu ZHANG, Minghui HE, Shanggao LIAO, Yongqin ZENG, and Yan LIN
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CHEMICAL processes ,POLYGONUM ,CRYSTAL whiskers ,PLANT genomes ,POLLEN - Abstract
To investigate the systematic evaluation of pharmacognostic identification of Polygonum capitatum. [Methods] 10 batches of P. capitatum cultivated in Guizhou were chosen for plant samples. Macroscopical identification was conducted on plant roots, stems, leaves, flowers and fruits. The P. capitatum powder was processed for physical and chemical distinction by FeCl3 chromogenic reaction, hydrochloric acid magnesium powder reaction, A1C13 color development reaction and thin-layer chromatography. Microscope identification was carried out on the powder. Plant genome DNeasy Plant Kit was adopted for DNA molecular marker identification. [Results] The results showed that the stem of P. capitatum was tufted, the leaves were oval, 2 to 5 cm long, and 1 to 2 cm wide; the leaf apex was acute and cuneate at the base, the inflorescence was capitate, paired or solitary; the raceme was erect and nearly spherical, and the perianth was light red. Furthermore, for the chromogenic reaction of FeCl3 ethanol extract of P. capitatum, appeared blue and turned to dark blue after long time storing at room temperature. For the reaction of hydrochloric acid magnesium powder, the alcohol extract of P. capitatum, exhibited deep red. In the color reaction of A1C1, the alcohol extract revealed yellow fluorescence under 360 nm UV lamp. Microscope identification of the powder displayed pollen grains, crystal sheath fibers, cellulose, vessels, starch grains, cork cells, and other characteristic fragments. In addition, DNA barcoding electrophoresis results showed that P. capitatum showed a clear and bright single band near 500 bp, and further sequencing results showed that the sequence differences were mainly concentrated in 1TS1 and 1TS2 region. [Conclusions] Systematic evaluation for the identification of P. capitatum is established, which combines with macroscopic identification, physicochemical identification, powder microscope identification, and DNA molecular identification. Finally, the original medicinal material is identified as P. capitatum Buch. -Ham. ex D. Don. [ABSTRACT FROM AUTHOR]
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- 2023
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35. 3D Photoprintable Antistatic Materials with Polymerizable Deep Eutectic Solvents
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Jiahui Su, Shaohui Li, Yonglong Chen, Yanyan Cui, and Minghui He
- Subjects
General Chemical Engineering ,General Chemistry ,Industrial and Manufacturing Engineering - Published
- 2021
36. Mechanistic insight of SARS-CoV-2 infection using human hepatobiliary organoids
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Yi Zhao, Xiaoxue Ren, Jing Lu, Minghui He, Zhe Liu, Lina Yi, Mingle Huang, Ming Kuang, Haipeng Xiao, Joseph JY Sung, Xiaoxing Li, Lixia Xu, and Jun Yu
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Gastroenterology - Published
- 2022
37. Research on design of intersection signal light based on artificial intelligence algorithm
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Minghui He
- Published
- 2022
38. Improvement of GCC Congestion Control Algorithm in Streaming Media Transmission
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Minghui He, Yan Yuan, Kang Li, and Han Peng
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- 2022
39. Distinct Single-cell Immune Ecosystems Distinguish True and De Novo HBV-related Hepatocellular Carcinoma Recurrences
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Shuling Chen, Cheng Huang, Guanrui Liao, Huichuan Sun, Yubin Xie, Jianping Wang, Minghui He, Huanjing Hu, Zihao Dai, Xiaoxue Ren, Xuezhen Zeng, Qianwen Zeng, Guopei Zhang, Changyi Liao, Wenxuan Xie, Shunli Shen, Shaoqiang Li, Sui Peng, Dongming Kuang, Qiang Zhao, Dan G. Duda, and Ming Kuang
- Abstract
SummaryRevealing differential tumor immune microenvironment (TIME) characteristics between true versus de novo hepatocellular carcinoma (HCC) recurrence could help optimal development and use of immunotherapies. Here, we studied the TIME of recurrent HBV-related HCCs by 5’and VDJ single-cell and bulk RNA-sequencing, flow cytometry, and multiplexed immunofluorescence. Analyses of mutational profiles, evolutionary trajectories, and clonal architecture using whole-exome sequencing identified de novo versus true recurrences, some of which occurred before clinical diagnosis. The TIME of truly recurrent HCCs was characterized by an increased abundance in KLRB1+CD8+ T cells with memory phenotype and low cytotoxicity. In contrast, we found an enrichment in cytotoxic and exhausted CD8+ T cells in the TIME of de novo recurrent HCCs. Transcriptomic and interaction analyses showed an upregulated GDF15 expression level on HCC cells in proximity to dendritic cells, which may have dampened antigen presentation and inhibited anti-tumor immunity in the TIME of truly recurrent lesions. In contrast, we found that myeloid cells’ crosstalk with T cells mediated T cell exhaustion and immunosuppression in the TIME of de novo recurrent HCC. In conclusion, our results support genomic diagnosis and immune profiling for guiding immunotherapy implementation based on the type of HCC recurrence and TIME.HighlightsTruly recurrent lesions are seeded before primary tumor diagnosis, and that de novo cancer can occur earlier than the clinically used 2-year limit.ScRNA-seq unravels distinct immune ecosystems in true versus de novo HCC recurrences, highlighting the need for different immunotherapy strategies for two types of HCC recurrence.CD8+ T cells in de novo recurrence displayed cytotoxic and exhausted phenotypes while those in truly recurrent lesions showed a memory phenotype with weak cytotoxicity.HCC cells expressing the inhibitory molecule GDF15 were in the proximity of DCs only in truly recurrent lesions.High GDF15 expression level was associated with truly recurrent HCC and worse prognosis.Graphical abstract
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- 2022
40. Overactive WASp in X-linked neutropenia leads to aberrant B-cell division and accelerated plasma cell generation
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E.A. Deordieva, Lieselot Buedts, Lisa S. Westerberg, Chaohong Liu, Roberta D’Aulerio, Julien Record, Mezida B. Saeed, Peter Vandenberghe, Siobhan O. Burns, Lennart Hammarström, Marton Keszei, Larissa Vasconcelos-Fontes, Yu Xia, Chiara Geyer, Mariana M.S. Oliveira, Charlotte Cunningham-Rundles, Anna Shcherbina, Minghui He, Vinicius Cotta-de-Almeida, Lia Gonçalves Pinho, Lena Bohaumilitzky, Meike Thiemann, Dmitry Pershin, Rhaissa Vieira, Joao Pedro Lopes, Xiaodong Zhao, and Adrian J. Thrasher
- Subjects
Neutropenia ,Wiskott–Aldrich syndrome ,Plasma Cells ,Immunology ,Naive B cell ,macromolecular substances ,Plasma cell ,Biology ,primary immunodeficiency ,plasma cells ,Affinity maturation ,Mice ,X-linked neutropenia ,medicine ,Animals ,Humans ,Immunology and Allergy ,B cell ,B-Lymphocytes ,B cells ,Cell growth ,Germinal center ,Genetic Diseases, X-Linked ,WASp ,medicine.disease ,Molecular biology ,Immunoglobulin A ,medicine.anatomical_structure ,Immunoglobulin class switching ,germinal center ,actin ,Cell Division ,Wiskott-Aldrich Syndrome Protein ,IgA - Abstract
BACKGROUND: B-cell affinity maturation in germinal center relies on regulated actin dynamics for cell migration and cell-to-cell communication. Activating mutations in the cytoskeletal regulator Wiskott-Aldrich syndrome protein (WASp) cause X-linked neutropenia (XLN) with reduced serum level of IgA. OBJECTIVE: We investigated the role of B cells in XLN pathogenesis. METHODS: We examined B cells from 6 XLN patients, 2 of whom had novel R268W and S271F mutations in WASp. By using immunized XLN mouse models that carry the corresponding patient mutations, WASp L272P or WASp I296T, we examined the B-cell response. RESULTS: XLN patients had normal naive B cells and plasmablasts, but reduced IgA+ B cells and memory B cells, and poor B-cell proliferation. On immunization, XLN mice had a 2-fold reduction in germinal center B cells in spleen, but with increased generation of plasmablasts and plasma cells. In vitro, XLN B cells showed reduced immunoglobulin class switching and aberrant cell division as well as increased production of immunoglobulin-switched plasma cells. CONCLUSIONS: Overactive WASp predisposes B cells for premature differentiation into plasma cells at the expense of cell proliferation and immunoglobulin class switching. ispartof: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY vol:149 issue:3 pages:1069-1084 ispartof: location:United States status: published
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- 2022
41. Tailoring the Pore Surface of 3D Covalent Organic Frameworks via Post‐Synthetic Click Chemistry
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Cheng Wang, Bo Gui, Xuefen Liu, Ya Zhang, Pohua Chen, Yuanpeng Cheng, Junliang Sun, and Minghui He
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Materials science ,Covalent bond ,Click chemistry ,Nanotechnology ,General Chemistry ,Gas separation ,General Medicine ,Surface engineering ,Catalysis ,Characterization (materials science) - Abstract
Three-dimensional covalent organic frameworks (3D COFs) have gained increasing attention for their attractive features. However, the development of 3D COFs is strongly restricted, mainly due to their synthetic difficulty and complicated structure determination. Post-synthetic modification, which can avoid these problems by incorporating functional moieties into a predetermined framework, provides an alternative way to construct 3D COFs with specific functions. Herein, we report the designed synthesis and characterization of a series of highly crystalline 3D COFs with different loadings of ethynyl groups. Notably, these alkyne-tagged 3D COFs provide a platform for targeted anchoring various specific groups onto the pore walls via click reactions. Moreover, the pore surface engineering can accordingly change their properties, for example, the obtained click products exhibited higher CO2 /N2 selectivity. We describe a simple but powerful strategy to build functional 3D COFs, which will certainly advance them for a ranging of interesting applications in the future.
- Published
- 2021
42. Research on the Formulation of Rural Planning from the Perspective of Integration of Agriculture, Culture and Tourism: A Case Study of Guifengshan Village, Macheng City, Hubei Province
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Rui Wang, Hong Xu, Xuecheng Hou, and Minghui He
- Subjects
Geography ,Agriculture ,business.industry ,Perspective (graphical) ,Regional science ,business ,Tourism - Abstract
With the gradual construction of the land and space planning system and the acceleration of the urban-rural integrated development process, China’s rural land planning and preparation have ushered in substantial innovations, and at the same time, they are also facing huge challenges. Among them, the low utilization rate of rural land and cultural desertification are the crux of the lagging rural development. This paper takes Guifengshan Village in Macheng City, Hubei Province as an example. Through the analysis of the current advantages of the village, the analysis of challenges, and the evaluation of the suitability of land use, this paper proposes to promote the mutual benefit of agriculture, culture and tourism, and promote the revitalization of Guifengshan Village, forming a “agricultural industry + characteristic cultural creation + red ecology”. “Tourism” jointly develops rural planning strategies. It aims to link rural agriculture, tourism and cultural industries closely on the basis of people-oriented and ecological protection, with income generation as the goal of rural revitalization, to achieve the establishment and extension of the industrial chain, and to promote rural revitalization.
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- 2021
43. Relationship between cumulative exposure to metal mixtures and heart rate among Chinese preschoolers
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Ye Fu, Yun Liu, Yanli Liu, Yan Wang, Meiqin Zhu, Wei Lin, Mingzhu Li, Yang Liu, Minghui He, Lili Yu, and Jing Wang
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China ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Pollution ,Arsenic ,Zinc ,Heart Rate ,Metals, Heavy ,Humans ,Environmental Chemistry ,Child - Abstract
The cumulative exposure to metals affects cardiac conduction, and the effect of polymetallic exposure on heart rate in children is unknown. To evaluate the relationship between cumulative exposure to metal mixtures and heart rate among Chinese preschoolers, the determination of urinary 24 metals was processed by high-resolution inductively coupled plasma-mass spectrometry. Heart rate was recorded when measuring blood pressure after resting 5 min or longer. As a method to compute the Environmental risk score (ERS) according to heart rate under heavy metal mixtures, adaptive elastic net (AENET) with 299 predictors which were formed by the combination of main effects, squared terms, and pairwise interactions of heavy metals with a total number of 23, 23, 253 respectively. To further assess the associations between ERS and heart rate, regression analyses were performed with complex survey designs. The construction of ERS under heart rate-related metal mixtures was returned by AENET in according to 11 main effects (tin, arsenic, zinc, iron, titanium, vanadium, nickel, manganese, cobalt, copper and chromium) and 2 squared terms (tungsten and rubidium). A high correlation was monitored between the alteration of ERS in the study population and heart rate (β = 1.030, 95% CI: 0.730 - 1.330 in 1239; β = 1.085, 95% CI: 0.777 - 1.393 in 1061). Significant associations of ERS with higher heart rates were also pointed out (Ps 0.05). Our study elucidates the association of the cumulative exposure of heavy metals as mixtures and heart rate among Chinese preschoolers. Further research is obliged to corroborate these findings in longitudinal studies.
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- 2022
44. Graphene-assisted construction of electrocatalysts for carbon dioxide reduction
- Author
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Yugang Huang, Hong Zhao, Yinlei Lin, Yuyuan Zhang, Huawen Hu, Dongchu Chen, Jian Zhen Ou, Minghui He, Xuejun Xu, and Lifang Deng
- Subjects
Materials science ,Graphene ,General Chemical Engineering ,chemistry.chemical_element ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Redox ,Industrial and Manufacturing Engineering ,0104 chemical sciences ,law.invention ,Catalysis ,Reaction rate ,chemistry ,law ,Specific surface area ,Environmental Chemistry ,0210 nano-technology ,Carbon ,Electrochemical reduction of carbon dioxide - Abstract
The electrochemical conversion of the greenhouse gas, carbon dioxide (CO2), to energy fuels and value-added chemicals presents one of the most valuable approaches to harvest pollutants and produce renewable energy. However, the stable molecular structure of CO2 and the sluggish reaction kinetics make CO2 reduction reaction (CO2RR) formidably challenging to achieve reaction rate and selectivity practical in industry. Graphene and its derivatives have been considered a group of intriguing materials to develop advanced CO2RR electrocatalysts due to their large specific surface area, remarkable electron transfer ability, superior stability, and easy tunability of the structure and surface properties. Herein, we comprehensively discuss the state-of-the-art electrocatalysts constructed with graphene and derivatives for active and selective CO2RR within the recent five years, mainly including the electrocatalysts with both metal-based (e.g., noble, non-noble, or combined thereof) and non-metal (e.g., doped, modified, defected, or composited) catalytic sites. To present the versatile, high-performance metal-based CO2RR electrocatalysts constructed with graphene, we further subdivide them according to the sizes, oxidation states, metal species synergies, dimensionalities, and versatility. Finally, we provide the challenges and perspectives in this emerging area of utilising CO2 to produce various carbon-based fuels and chemicals via graphene chemistry.
- Published
- 2021
45. 3D printing of ultra-tough, self-healing transparent conductive elastomeric sensors
- Author
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Guangxue Chen, Minghui He, Bin Su, and Ling Cai
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Materials science ,business.industry ,General Chemical Engineering ,Soft robotics ,3D printing ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Elastomer ,01 natural sciences ,Industrial and Manufacturing Engineering ,0104 chemical sciences ,chemistry.chemical_compound ,Photopolymer ,Polymerization ,chemistry ,Self-healing ,Environmental Chemistry ,Electronics ,0210 nano-technology ,business ,Choline chloride - Abstract
Ultra-tough transparent conductive elastomers (TCEs) with self-healing performance are of great significance for research in the fields of soft robotics, optical display electronics, wearable electronic equipment and human–computer interaction. However, their limitation, such as the desolventizing process, low polymerization rate or balance between toughness and self-healing performance, cause difficulty in manufacturing by the 3D printing technique. Here, we propose a novel preparation strategy for 3D printed TCEs based on the photopolymerization of maleic acid (MA)/choline chloride (ChCl) and acrylamide (AAm)/choline chloride type polymerizable deep eutectic solvents (PDESs). PDES can polymerize into a tough and rich in hydrogen bonds poly(PDES) in a few seconds without solvent evaporation. The printed TCEs with precision of 10 µm have high transparency (95.6%) and stable electrical signals at 50% compression for 10,000 cycles. In addition, efficient self-healing performance due to the dynamic hydrogen bonds of poly(PDES) provides broad application prospects for 3D printed TCEs in long-term pressure-resistant intelligent applications.
- Published
- 2021
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