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Abstract Desmodesmus spp. are one of the most dominant components of phytoplankton, which are present in most water bodies. However, identification of the species based only on morphological data is challenging. The aim of the present study was to provide a comprehensive understanding of the actual distribution of the Desmodesmus species in Saga City, Saga Prefecture, Japan. In the present study, 38 water bodies were surveyed between June 2017 and March 2023. A total of 86 culture strains were established from the samples collected from the 21 sites, and identified by molecular phylogenetic analysis, comparison of ITS2 rRNA secondary structures, and observation of surface microstructure. In total, four new species, including D. notatus Demura sp. nov., D. lamellatus Demura sp. nov., D. fragilis Demura sp. nov., and D. reticulatus Demura sp. nov. were proposed and 17 Desmodesmus species were identified as described species. The present study revealed > 20 Desmodesmus species, exhibiting high genetic diversity in a small area.

Fish sauce is produced at high salt concentrations (>20%) to inhibit the growth of harmful microorganisms. The salt-reduced fish sauce (10% salt) was prepared under pressurized CO2 (pCO2) conditions at 30 °C and 5 MPa for 3 months (FSCO2), from Sardinops melanostictus, Odontamblyopus lacepedii, Trachurus japonicus, Konosirus punctatus, and their collective mixture, as well as unused fish mixture obtained from the Ariake Sea in Japan. FSCO2 exhibited significantly better microbial quality and free amino acid content, lighter color, standardized odor (dashi-like odor), and umami richness qualities compared to fish sauces prepared using the conventional method (FScon) (20% salt), as previously demonstrated, after a fermentation period of 2 months. Bacterial flora analysis implied that the standardization of odor and umami richness may not be the result of specific microbial metabolism. Even when using previously unused fish, it was possible to produce FSCO2 equivalent to that produced by conventional sardines and other fish. These results indicate that the quality of fish sauce can be improved. The flavor of FSCO2 became similar regardless of the type of fish and fermentation period using pCO2 during fermentation, leading to the effective utilization of unutilized fish as a resource for high-quality salt-reduced fish sauce.

Microalgae are now known as potential microorganisms in the production of chemicals, fuel, and food. Since microalgae live in the sea and the river, they need to be harvested and separated and cultured for further usage. In this study, to separate microalgae, a bed of magnetite-containing gel (Mag gel, 190 µm) was packed in the column by the application of a magnetic field for the separative elution of injected microalgae (including mainly four species), cultured at Saga University in Japan. The applied magnetic field was set at a constant and dynamic-convex manner. At a constant magnetic field of 0.4–1.1 T, the elution percentage of the microalgae at less than 5 µm was 30–50%. At 1.1 T, the larger-sized microalgae were eluted at a percentage of 20%, resulting in the structural change of the bed by the applied magnetic field. In a convex-like change of the magnetic field at 1.1 T ⇄ 0.4 T, the smaller-sized microalgae were selectively eluted, whereas at 1.1 T ⇄ 0.8 T, the larger-sized microalgae were eluted. Dynamic convex-like changes by the magnetic field selectively eluted the microalgae, leading to the separation and the extraction of potential microalgae.

Phycobiliprotein (PB), which is produced by cyanobacteria, has potential applications as a valuable pharmaceutical product. In this study, PB was separated by using a nonwoven-fabric membrane (Rx-1) that contained quaternary amino groups. PB was extracted from a Nostoc commune, which is a type of microalga, by disrupting the cell walls by a freeze–thaw method. Two types of biomolecules, namely PB and other biomolecules X, were identified by gel permeation chromatography. Permeation of the PB-containing solution through Rx-1 increased the purity of the PB in the filtrate from 0.25 to 0.45. The adsorption constant (Kp) and maximum adsorbed amount of each protein on Rx-1 (qmax) were determined by fitting the experimental data to a mathematical model. The obtained values for Kp and qmax were used to simulate scaled-up treatment of a PB-containing solution. Permeation of a PB-containing solution (3.0 L at 1.2 × 10−3 m³/h) through Rx-1 (volume of scale-up membrane, 8.0 × 10−4 m³) enabled PB separation for 26 h. Further PB purification could be achieved by using the Rx-1 membrane in combination with precipitation with ammonium sulfate solution and an ultrafiltration membrane. [ABSTRACT FROM AUTHOR]