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To explore the effect of recombinant human growth hormone on burn wound healing by regulating vascular endothelial growth factor and micro vessel density. Sixty rats were randomly divided into control group (n=20) (group A), low dose recombinant human growth hormone intervention group (n=20) (group B) and high dose recombinant human growth hormone intervention group (n=20) (group C). After the deep II burn wounds were formed in all rats, 1.0 ml saline was injected into the back burn wounds in the group A, 1.0 ml of 0.2 IU/ml recombinant human growth hormone saline solution was injected into the back burn wounds in the low dose recombinant human growth hormone intervention group, and 0.4 IU/ml recombinant human growth hormone saline solution was injected into the back burn wounds in the group C every day. The time of scab removal and hair growth, vascular endothelial growth factor expression, micro vessel density, oxidative stress index and inflammation index were compared in each group. Rats in group B showed shorter decrustation and hair growth times than those in group A, while rats in group C showed shorter decrustation and hair growth times. In group A, vascular endothelial growth factor expression was higher than in group B, and in group C, vascular endothelial growth factor expression was higher than in group B. In group B, the level of micro vessel density was higher than in group A, and in group C, it was higher than in group A. Superoxide dismutase levels in the group B were higher than those in the group A, and malondialdehyde levels in the group C were higher than those in the group B, while malondialdehyde levels in the group C were lower than those in the group A. As compared to group A, the levels of interleukin-6, tumor necrosis factor-alpha, and interleukin-1 alpha were reduced in group B, while in group C they were decreased. Recombinant human growth hormone can promote burn wound repair by regulating vascular endothelial growth factor and micro vessel density. [ABSTRACT FROM AUTHOR]

Introduction: Psoriasis is a chronic inflammatory immunemediated cutaneous disorder. Histologically, it shows epidermal proliferation and neovascularisation in papillary dermis. Histological grading of psoriasis is done using Trozak Histological assessment Score (THS). Angiogenesis drives psoriasis. Vascular Endothelial Growth Factor (VEGF) and Cluster of Differentiation (CD34)/Micro Vessel Density (MVD) are proangiogenic cytokines in psoriasis and are overexpressed in psoriatic skin when compared to normal controls. Aim: 1) To analyse and compare the IHC expression of VEGF and CD34/MVD in skin biopsies of psoriasis cases and controls; 2) To compare the expression of VEGF, CD34/MVD with histological grade done by THS. Materials and Methods: A retrospective study was done in the Department of Pathology at Dr. B.R. Ambedkar Medical College and Hospital, Bengaluru, Karnataka, India, between September 2020 to August 2021. Twenty five cases and 25 controls (normal skin) were included in the present study. Histopathology/Immunohistochemistry (IHC) for VEGF and CD34 was performed. Suprabasilar layers of the epidermis were assessed for VEGF expression. Cell showing granular positivity within the cytoplasm was considered positive for VEGF. The mean percentage of positive cells was determined in a minimum of five areas at X400 magnification. CD34/MVD was calculated as the number of vessels in three high density locations. The MVD/High Power Field (HPF) for each individual lesion and graded as 21 per HPF. The THS was done as per standard protocol. Results: The age range in the present study was 18-68 years with male:female ratio of 1.2:1. An increased VEGF expression (p-value

Background: The role of the basic fibroblast growth factor (bFGF) has well known in the angiogenesis and ulcer healing. In this study, we aimed to evaluate the effects of bFGF on tissue repair in a rat oral mucosal wound. Methods: Musosal wound induced on the lip mucosa of rats and bFGF was injected along the edge of the mucosal defect immediately after surgery. The tissues were collected on days 3, 7 and 14 after the wound induction. The micro vessel density (MVD) and CD34 expression were done by histochemical studies. Results: The bFGF significantly accelerated granulation tissue formation and MVD was increased three days after ulcer induction but decreased 14 days after surgery. The MVD was significantly higher in the bFGF-treated group. The wound area was decreased in all groups time-dependently and a statistically significant difference (p value?) was observed between the bFGF-treated group and untreated group. The wound area was smaller in the bFGF-treated group compared to the untreated group. Conclusions: Our data demonstrated that bFGF can accelerated and facilitated wound healing. [ABSTRACT FROM AUTHOR]

Introduction. Neoplastic microenvironment represents a frequently studied subject in numerous studies. Angiogenesis, as a part of it, is a prognostic factor and a therapeutic target in several neoplastic diseases. Cases presentation. We describe the angiogenetic process in a small series of extra gastrointestinal stromal tumours (EGISTs) and corelate it with the proliferation index. We retrospectively searched our institutions databases for EGIST cases. The resulting data were reviewed by two pathologists. Immunohistochemistry (IHC) was performed to highlight the vascular structure of the tumours and determine the proliferative index using ki67 staining. Subsequently, the whole slide scanned digital images were used to assess micro vessel density and vascular surface. Statistical analysis of measurements data yielded a negative correlation between the tumour proliferative index and vascular surface normalized to examined surface of the tumour (r=-0.5506 and p=0.0051). A correlation between micro vessel density and proliferative index of the tumour could not be established in our series (p =0.035). Conclusions. Highly proliferative EGISTs have a reduced micro vessel surface compared to low grade tumours, possibly due to a limited development rate of the angiogenesis during the tumours’ growth. Due to the rarity of this sarcoma, our study is limited by the small number of cases, as well as by the preanalytical procedures performed on the tissue that may alter the vascular area in different manners. Further studies on larger series are needed to confirm our results and to investigate other morphological parameters, to assess the utility of anti-angiogenic therapies.