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1. Introduction and Spread of Dengue Virus 3, Florida, USA, May 2022-April 2023

Author

Jones, Forrest K., Morrison, Andrea M., Santiago, Gilberto A., Rysava, Kristyna, Zimler, Rebecca A., Heberlein, Lea A., Kopp, Edgar, Team, Florida Department of Health Bureau of Public Health Laboratory, Saunders, Katharine E., Baudin, Samantha, Rico, Edhelene, Mejia-Echeverri, Alvaro, Taylor-Salmon, Emma, Hill, Verity, Breban, Mallery I., Vogels, Chantal B.F., Grubaugh, Nathan D., Paul, Lauren M., Michael, Scott F., Johansson, Michael A., Adams, Laura E., Munoz-Jordan, Jorge, Paz-Bailey, Gabriela, and Stanek, Danielle R.

Subjects
United States. Centers for Disease Control and Prevention, Genomics -- Health aspects, Infection -- Health aspects, Travelers -- Health aspects, Dengue viruses -- Health aspects, Health
Abstract

Dengue virus (DENV) is not endemic in the continental United States (1); most cases occur among travelers to DENV-endemic areas (2). In Florida, USA, DENV infections are primarily reported among [...]

Published
2024
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2. Instructional Models for Course-Based Research Experience (CRE) Teaching

Author

Hanauer, David I, Graham, Mark J, Arnold, Rachel J, Ayuk, Mary A, Balish, Mitchell F, Beyer, Andrea R, Butela, Kristen A, Byrum, Christine A, Chia, Catherine P, Chung, Hui-Min, Clase, Kari L, Conant, Stephanie, Coomans, Roy J, D’Elia, Tom, Diaz, Jason, Diaz, Arturo, Doty, Jean A, Edgington, Nicholas P, Edwards, Dustin C, Eivazova, Elvira, Emmons, Christine B, Fast, Kayla M, Fisher, Emily J, Fleischacker, Christine L, Frederick, Gregory D, Freise, Amanda C, Gainey, Maria D, Gissendanner, Chris R, Golebiewska, Urszula P, Guild, Nancy A, Hendrickson, Heather L, Herren, Christopher D, Hopson-Fernandes, Margaret S, Hughes, Lee E, Jacobs-Sera, Deborah, Johnson, Allison A, Kirkpatrick, Bridgette L, Klyczek, Karen K, Koga, Ann P, Kotturi, Hari, LeBlanc-Straceski, Janine, Lee-Soety, Julia Y, Leonard, Justin E, Mastropaolo, Matthew D, Merkhofer, Evan C, Michael, Scott F, Mitchell, Jon C, Mohan, Swarna, Monti, Denise L, Noutsos, Christos, Nsa, Imade Y, Peters, Nick T, Plymale, Ruth, Pollenz, Richard S, Porter, Megan L, Rinehart, Claire A, Rosas-Acosta, German, Ross, Joseph F, Rubin, Michael R, Scherer, Anne E, Schroeder, Stephanie C, Shaffer, Christopher D, Sprenkle, Amy B, Sunnen, C Nicole, Swerdlow, Sarah J, Tobiason, Deborah, Tolsma, Sara S, Tsourkas, Philippos K, Ward, Robert E, Ware, Vassie C, Warner, Marcie H, Washington, Jacqueline M, Westover, Kristi M, White, Simon J, Whitefleet-Smith, JoAnn L, Williams, Daniel C, Wolyniak, Michael J, Zeilstra-Ryalls, Jill H, Asai, David J, Hatfull, Graham F, and Sivanathan, Viknesh

Subjects
Curriculum and Pedagogy, Education Systems, Education, Quality Education, Engineering, Faculty, Humans, Mathematics, Models, Educational, Students, Teaching, Curriculum and pedagogy
Abstract

The course-based research experience (CRE) with its documented educational benefits is increasingly being implemented in science, technology, engineering, and mathematics education. This article reports on a study that was done over a period of 3 years to explicate the instructional processes involved in teaching an undergraduate CRE. One hundred and two instructors from the established and large multi-institutional SEA-PHAGES program were surveyed for their understanding of the aims and practices of CRE teaching. This was followed by large-scale feedback sessions with the cohort of instructors at the annual SEA Faculty Meeting and subsequently with a small focus group of expert CRE instructors. Using a qualitative content analysis approach, the survey data were analyzed for the aims of inquiry instruction and pedagogical practices used to achieve these goals. The results characterize CRE inquiry teaching as involving three instructional models: 1) being a scientist and generating data; 2) teaching procedural knowledge; and 3) fostering project ownership. Each of these models is explicated and visualized in terms of the specific pedagogical practices and their relationships. The models present a complex picture of the ways in which CRE instruction is conducted on a daily basis and can inform instructors and institutions new to CRE teaching.

Published
2022

3. Instructional Models for Course-Based Research Experience (CRE) Teaching

Author

Hanauer, David I., Graham, Mark J., Arnold, Rachel J., Ayuk, Mary A., Balish, Mitchell F., Beyer, Andrea R., Butela, Kristen A., Byrum, Christine A., Chia, Catherine P., Chung, Hui-Min, Clase, Kari L., Conant, Stephanie, Coomans, Roy J., D'Elia, Tom, Diaz, Jason, Diaz, Arturo, Doty, Jean A., Edgington, Nicholas P., Edwards, Dustin C., Eivazova, Elvira, Emmons, Christine B., Fast, Kayla M., Fisher, Emily J., Fleischacker, Christine L., Frederick, Gregory D., Freise, Amanda C., Gainey, Maria D., Gissendanner, Chris R., Golebiewska, Urszula P., Guild, Nancy A., Hendrickson, Heather L., Herren, Christopher D., Hopson-Fernandes, Margaret S., Hughes, Lee E., Jacobs-Sera, Deborah, Johnson, Allison A., Kirkpatrick, Bridgette L., Klyczek, Karen K., Koga, Ann P., Kotturi, Hari, LeBlanc-Straceski, Janine, Lee-Soety, Julia Y., Leonard, Justin E., Mastropaolo, Matthew D., Merkhofer, Evan C., Michael, Scott F., Mitchell, Jon C., Mohan, Swarna, Monti, Denise L., Noutsos, Christos, Nsa, Imade Y., Peters, Nick T., Plymale, Ruth, Pollenz, Richard S., Porter, Megan L., Rinehart, Claire A., Rosas-Acosta, German, Ross, Joseph F., Rubin, Michael R., Scherer, Anne E., Schroeder, Stephanie C., Shaffer, Christopher D., Sprenkle, Amy B., Sunnen, C. Nicole, Swerdlow, Sarah J., Tobiason, Deborah, Tolsma, Sara S., Tsourkas, Philippos K., Ward, Robert E., Ware, Vassie C., Warner, Marcie H., Washington, Jacqueline M., Westover, Kristi M., White, Simon J., Whitefleet-Smith, JoAnn L., Williams, Daniel C., Wolyniak, Michael J., Zeilstra-Ryalls, Jill H., Asai, David J., Hatfull, Graham F., and Sivanathan, Viknesh

Abstract

The course-based research experience (CRE) with its documented educational benefits is increasingly being implemented in science, technology, engineering, and mathematics education. This article reports on a study that was done over a period of 3 years to explicate the instructional processes involved in teaching an undergraduate CRE. One hundred and two instructors from the established and large multi-institutional SEA-PHAGES program were surveyed for their understanding of the aims and practices of CRE teaching. This was followed by large-scale feedback sessions with the cohort of instructors at the annual SEA Faculty Meeting and subsequently with a small focus group of expert CRE instructors. Using a qualitative content analysis approach, the survey data were analyzed for the aims of inquiry instruction and pedagogical practices used to achieve these goals. The results characterize CRE inquiry teaching as involving three instructional models: (1) being a scientist and generating data; (2) teaching procedural knowledge; and (3) fostering project ownership. Each of these models is explicated and visualized in terms of the specific pedagogical practices and their relationships. The models present a complex picture of the ways in which CRE instruction is conducted on a daily basis and can inform instructors and institutions new to CRE teaching.

Published
2022
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4. DengueSeq: a pan-serotype whole genome amplicon sequencing protocol for dengue virus

Author

Vogels, Chantal B. F., primary, Hill, Verity, additional, Breban, Mallery I., additional, Chaguza, Chrispin, additional, Paul, Lauren M., additional, Sodeinde, Afeez, additional, Taylor-Salmon, Emma, additional, Ott, Isabel M., additional, Petrone, Mary E., additional, Dijk, Dennis, additional, Jonges, Marcel, additional, Welkers, Matthijs R. A., additional, Locksmith, Timothy, additional, Dong, Yibo, additional, Tarigopula, Namratha, additional, Tekin, Omer, additional, Schmedes, Sarah, additional, Bunch, Sylvia, additional, Cano, Natalia, additional, Jaber, Rayah, additional, Panzera, Charles, additional, Stryker, Ian, additional, Vergara, Julieta, additional, Zimler, Rebecca, additional, Kopp, Edgar, additional, Heberlein, Lea, additional, Herzog, Kaylee S., additional, Fauver, Joseph R., additional, Morrison, Andrea M., additional, Michael, Scott F., additional, and Grubaugh, Nathan D., additional

Published
2024
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5. Travel surveillance uncovers dengue virus dynamics and introductions in the Caribbean

Author

Taylor-Salmon, Emma, primary, Hill, Verity, additional, Paul, Lauren M., additional, Koch, Robert T., additional, Breban, Mallery I., additional, Chaguza, Chrispin, additional, Sodeinde, Afeez, additional, Warren, Joshua L., additional, Bunch, Sylvia, additional, Cano, Natalia, additional, Cone, Marshall, additional, Eysoldt, Sarah, additional, Garcia, Alezaundra, additional, Gilles, Nicadia, additional, Hagy, Andrew, additional, Heberlein, Lea, additional, Jaber, Rayah, additional, Kassens, Elizabeth, additional, Colarusso, Pamela, additional, Davis, Amanda, additional, Baudin, Samantha, additional, Rico, Edhelene, additional, Mejía-Echeverri, Álvaro, additional, Scott, Blake, additional, Stanek, Danielle, additional, Zimler, Rebecca, additional, Muñoz-Jordán, Jorge L., additional, Santiago, Gilberto A., additional, Adams, Laura E., additional, Paz-Bailey, Gabriela, additional, Spillane, Melanie, additional, Katebi, Volha, additional, Paulino-Ramírez, Robert, additional, Mueses, Sayira, additional, Peguero, Armando, additional, Sánchez, Nelissa, additional, Norman, Francesca F., additional, Galán, Juan-Carlos, additional, Huits, Ralph, additional, Hamer, Davidson H., additional, Vogels, Chantal B. F., additional, Morrison, Andrea, additional, Michael, Scott F., additional, and Grubaugh, Nathan D., additional

Published
2024
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6. Models of classroom assessment for course-based research experiences

Author

Hanauer, David I., primary, Zhang, Tong, additional, Graham, Mark J., additional, Adams, Sandra D., additional, Ahumada-Santos, Yesmi Patricia, additional, Alvey, Richard M., additional, Antunes, Mauricio S., additional, Ayuk, Mary A., additional, Elena Báez-Flores, María, additional, Bancroft, Christa T., additional, Bates, Tonya C., additional, Bechman, Meghan J., additional, Behr, Elizabeth, additional, Beyer, Andrea R., additional, Bortz, Rebecca L., additional, Bowder, Dane M., additional, Briggs, Laura A., additional, Brown-Kennerly, Victoria, additional, Buckholt, Michael A., additional, Bullock, Sharon K., additional, Butela, Kristen A., additional, Byrum, Christine A., additional, Caruso, Steven M., additional, Chia, Catherine P., additional, Chong, Rebecca A., additional, Chung, Hui-Min, additional, Clase, Kari L., additional, Coleman, Sean T., additional, Parks Collins, D., additional, Conant, Stephanie B., additional, Condon, Brett M., additional, Connerly, Pamela L., additional, Connors, Bernadette J., additional, Cook-Easterwood, Jennifer E., additional, Crump, Katie E., additional, D’Elia, Tom, additional, Dennis, Megan K., additional, DeVeaux, Linda C., additional, Diacovich, Lautaro, additional, Duffy, Iain, additional, Edgington, Nicholas P., additional, Edwards, Dustin C., additional, Egwuatu, Tenny O. G., additional, Eivazova, Elvira R., additional, Fallest-Strobl, Patricia C., additional, Fillman, Christy L., additional, Findley, Ann M., additional, Fisher, Emily, additional, Fisher, Matthew R., additional, Fogarty, Marie P., additional, Freise, Amanda C., additional, Frost, Victoria J., additional, Gainey, Maria D., additional, Garcia Costas, Amaya M., additional, Garza, Atenea A., additional, Gavin, Hannah E., additional, Ghittoni, Raffaella, additional, Gibb, Bryan, additional, Golebiewska, Urszula P., additional, Grinath, Anna S., additional, Gurney, Susan M. R., additional, Hare, Rebekah F., additional, Heninger, Steven G., additional, Hinz, John M., additional, Hughes, Lee E., additional, Jayachandran, Pradeepa, additional, Johnson, Kristen C., additional, Johnson, Allison A., additional, Kanther, Michelle, additional, Kenna, Margaret, additional, Kirkpatrick, Bridgette L., additional, Klyczek, Karen K., additional, Kohl, Kathryn P., additional, Kuchka, Michael, additional, LaPeruta, Amber J., additional, Lee-Soety, Julia Y., additional, Lewis, Lynn O., additional, Lindberg, Heather M., additional, Madden, Jaclyn A., additional, Markov, Sergei A., additional, Mastropaolo, Matthew D., additional, Mathur, Vinayak, additional, McClory, Sean P., additional, Merkhofer, Evan C., additional, Merkle, Julie A., additional, Michael, Scott F., additional, Mitchell, Jon C., additional, Molloy, Sally D., additional, Monti, Denise L., additional, Mussi, María Alejandra, additional, Nance, Holly, additional, Nieto-Fernandez, Fernando E., additional, Nissen, Jillian C., additional, Nsa, Imade Y., additional, O’Donnell, Mary G., additional, Page, Shallee T., additional, Panagakis, Andrea, additional, Parra-Unda, Jesús Ricardo, additional, Pelletier, Tara A., additional, Perez Morales, Tiara G., additional, Peters, Nick T., additional, Phuntumart, Vipaporn, additional, Pollenz, Richard S., additional, Preuss, Mary L., additional, Puthoff, David P., additional, Raifu, Muideen K., additional, Reyna, Nathan S., additional, Rinehart, Claire A., additional, Rocheleau, Jessica M., additional, Rossier, Ombeline, additional, Rudner, Adam D., additional, Rueschhoff, Elizabeth E., additional, Ryan, Amy, additional, Saha, Sanghamitra, additional, Shaffer, Christopher D., additional, Smith, Mary Ann V., additional, Sprenkle, Amy B., additional, Strong, Christy L., additional, Sunnen, C. Nicole, additional, Tarbox, Brian P., additional, Temple, Louise, additional, Thoemke, Kara R., additional, Thomas, Michael A., additional, Tobiason, Deborah M., additional, Tolsma, Sara S., additional, Garcia, Julie Torruellas, additional, Valentine, Megan S., additional, Vazquez, Edwin, additional, Ward, Robert E., additional, Ward, Catherine M., additional, Ware, Vassie C., additional, Warner, Marcie H., additional, Washington, Jacqueline M., additional, Westholm, Daniel E., additional, Wheaton, Keith A., additional, Wilkes, Beth M., additional, Williams, Elizabeth C., additional, Biederman, William H., additional, Cresawn, Steven G., additional, Heller, Danielle M., additional, Jacobs-Sera, Deborah, additional, Hatfull, Graham F., additional, Asai, David J., additional, and Sivanathan, Viknesh, additional

Published
2023
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7. Travel surveillance uncovers dengue virus dynamics and introductions in the Caribbean

Author

Taylor-Salmon, Emma, primary, Hill, Verity, additional, Paul, Lauren M., additional, Koch, Robert T., additional, Breban, Mallery I., additional, Chaguza, Chrispin, additional, Sodeinde, Afeez, additional, Warren, Joshua L., additional, Bunch, Sylvia, additional, Cano, Natalia, additional, Cone, Marshall, additional, Eysoldt, Sarah, additional, Garcia, Alezaundra, additional, Gilles, Nicadia, additional, Hagy, Andrew, additional, Heberlein, Lea, additional, Jaber, Rayah, additional, Kassens, Elizabeth, additional, Colarusso, Pamela, additional, Davis, Amanda, additional, Baudin, Samantha, additional, Rico, Edhelene, additional, Mejia-Echeverri, Alvaro, additional, Scott, Blake, additional, Stanek, Danielle, additional, Zimler, Rebecca, additional, Munoz-Jordan, Jorge L., additional, Santiago, Gilberto A., additional, Adams, Laura E., additional, Paz-Bailey, Gabriela, additional, Spillane, Melanie, additional, Katebi, Volha, additional, Paulino-Ramirez, Robert, additional, Mueses, Sayira, additional, Peguero, Armando, additional, Sanchez, Nelissa, additional, Norman, Francesca F., additional, Galan, Juan-Carlos Galan, additional, Huits, Ralph Huits, additional, Hamer, Davidison H., additional, Vogels, Chantal B.F., additional, Morrison, Andrea Morrison, additional, Michael, Scott F., additional, and Grubaugh, Nathan D., additional

Published
2023
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8. DengueSeq: A pan-serotype whole genome amplicon sequencing protocol for dengue virus

Author

Vogels, Chantal B.F., primary, Hill, Verity, additional, Breban, Mallery I., additional, Chaguza, Chrispin, additional, Paul, Lauren M., additional, Sodeinde, Afeez, additional, Taylor-Salmon, Emma, additional, Ott, Isabel M., additional, Petrone, Mary E., additional, Dijk, Dennis, additional, Jonges, Marcel, additional, Welkers, Matthijs R.A., additional, Locksmith, Timothy, additional, Dong, Yibo, additional, Tarigopula, Namratha, additional, Tekin, Omer, additional, Schmedes, Sarah, additional, Bunch, Sylvia, additional, Cano, Natalia, additional, Jaber, Rayah, additional, Panzera, Charles, additional, Stryker, Ian, additional, Vergara, Julieta, additional, Zimler, Rebecca, additional, Kopp, Edgar, additional, Heberlein, Lea, additional, Morrison, Andrea M., additional, Michael, Scott F., additional, and Grubaugh, Nathan D., additional

Published
2023
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10. Travel surveillance uncovers dengue virus dynamics and introductions in the Caribbean.

Author

Taylor-Salmon E, Hill V, Paul LM, Koch RT, Breban MI, Chaguza C, Sodeinde A, Warren JL, Bunch S, Cano N, Cone M, Eysoldt S, Garcia A, Gilles N, Hagy A, Heberlein L, Jaber R, Kassens E, Colarusso P, Davis A, Baudin S, Rico E, Mejía-Echeverri Á, Scott B, Stanek D, Zimler R, Muñoz-Jordán JL, Santiago GA, Adams LE, Paz-Bailey G, Spillane M, Katebi V, Paulino-Ramírez R, Mueses S, Peguero A, Sánchez N, Norman FF, Galán JC, Huits R, Hamer DH, Vogels CBF, Morrison A, Michael SF, and Grubaugh ND

Abstract

Dengue is the most prevalent mosquito-borne viral disease in humans, and cases are continuing to rise globally. In particular, islands in the Caribbean have experienced more frequent outbreaks, and all four dengue virus (DENV) serotypes have been reported in the region, leading to hyperendemicity and increased rates of severe disease. However, there is significant variability regarding virus surveillance and reporting between islands, making it difficult to obtain an accurate understanding of the epidemiological patterns in the Caribbean. To investigate this, we used travel surveillance and genomic epidemiology to reconstruct outbreak dynamics, DENV serotype turnover, and patterns of spread within the region from 2009-2022. We uncovered two recent DENV-3 introductions from Asia, one of which resulted in a large outbreak in Cuba, which was previously under-reported. We also show that while outbreaks can be synchronized between islands, they are often caused by different serotypes. Our study highlights the importance of surveillance of infected travelers to provide a snapshot of local introductions and transmission in areas with limited local surveillance and suggests that the recent DENV-3 introductions may pose a major public health threat in the region.

Published
2023
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11. DengueSeq: A pan-serotype whole genome amplicon sequencing protocol for dengue virus.

Author

Vogels CBF, Hill V, Breban MI, Chaguza C, Paul LM, Sodeinde A, Taylor-Salmon E, Ott IM, Petrone ME, Dijk D, Jonges M, Welkers MRA, Locksmith T, Dong Y, Tarigopula N, Tekin O, Schmedes S, Bunch S, Cano N, Jaber R, Panzera C, Stryker I, Vergara J, Zimler R, Kopp E, Heberlein L, Morrison AM, Michael SF, and Grubaugh ND

Abstract

Background: The increasing burden of dengue virus on public health due to more explosive and frequent outbreaks highlights the need for improved surveillance and control. Genomic surveillance of dengue virus not only provides important insights into the emergence and spread of genetically diverse serotypes and genotypes, but it is also critical to monitor the effectiveness of newly implemented control strategies. Here, we present DengueSeq, an amplicon sequencing protocol, which enables whole-genome sequencing of all four dengue virus serotypes., Results: We developed primer schemes for the four dengue virus serotypes, which can be combined into a pan-serotype approach. We validated both approaches using genetically diverse virus stocks and clinical specimens that contained a range of virus copies. High genome coverage (>95%) was achieved for all genotypes, except DENV2 (genotype VI) and DENV 4 (genotype IV) sylvatics, with similar performance of the serotype-specific and pan-serotype approaches. The limit of detection to reach 70% coverage was 10 1 -10 2 RNA copies/μL for all four serotypes, which is similar to other commonly used primer schemes. DengueSeq facilitates the sequencing of samples without known serotypes, allows the detection of multiple serotypes in the same sample, and can be used with a variety of library prep kits and sequencing instruments., Conclusions: DengueSeq was systematically evaluated with virus stocks and clinical specimens spanning the genetic diversity within each of the four dengue virus serotypes. The primer schemes can be plugged into existing amplicon sequencing workflows to facilitate the global need for expanded dengue virus genomic surveillance., Competing Interests: Competing interests The authors declare that they have no competing interests.

Published
2023
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12. Instructional Models for Course-Based Research Experience (CRE) Teaching.

Author

Hanauer DI, Graham MJ, Arnold RJ, Ayuk MA, Balish MF, Beyer AR, Butela KA, Byrum CA, Chia CP, Chung HM, Clase KL, Conant S, Coomans RJ, D'Elia T, Diaz J, Diaz A, Doty JA, Edgington NP, Edwards DC, Eivazova E, Emmons CB, Fast KM, Fisher EJ, Fleischacker CL, Frederick GD, Freise AC, Gainey MD, Gissendanner CR, Golebiewska UP, Guild NA, Hendrickson HL, Herren CD, Hopson-Fernandes MS, Hughes LE, Jacobs-Sera D, Johnson AA, Kirkpatrick BL, Klyczek KK, Koga AP, Kotturi H, LeBlanc-Straceski J, Lee-Soety JY, Leonard JE, Mastropaolo MD, Merkhofer EC, Michael SF, Mitchell JC, Mohan S, Monti DL, Noutsos C, Nsa IY, Peters NT, Plymale R, Pollenz RS, Porter ML, Rinehart CA, Rosas-Acosta G, Ross JF, Rubin MR, Scherer AE, Schroeder SC, Shaffer CD, Sprenkle AB, Sunnen CN, Swerdlow SJ, Tobiason D, Tolsma SS, Tsourkas PK, Ward RE, Ware VC, Warner MH, Washington JM, Westover KM, White SJ, Whitefleet-Smith JL, Williams DC, Wolyniak MJ, Zeilstra-Ryalls JH, Asai DJ, Hatfull GF, and Sivanathan V

Subjects
Engineering, Faculty, Humans, Mathematics, Teaching, Models, Educational, Students
Abstract

The course-based research experience (CRE) with its documented educational benefits is increasingly being implemented in science, technology, engineering, and mathematics education. This article reports on a study that was done over a period of 3 years to explicate the instructional processes involved in teaching an undergraduate CRE. One hundred and two instructors from the established and large multi-institutional SEA-PHAGES program were surveyed for their understanding of the aims and practices of CRE teaching. This was followed by large-scale feedback sessions with the cohort of instructors at the annual SEA Faculty Meeting and subsequently with a small focus group of expert CRE instructors. Using a qualitative content analysis approach, the survey data were analyzed for the aims of inquiry instruction and pedagogical practices used to achieve these goals. The results characterize CRE inquiry teaching as involving three instructional models: 1) being a scientist and generating data; 2) teaching procedural knowledge; and 3) fostering project ownership. Each of these models is explicated and visualized in terms of the specific pedagogical practices and their relationships. The models present a complex picture of the ways in which CRE instruction is conducted on a daily basis and can inform instructors and institutions new to CRE teaching.

Published
2022
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