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Your search keyword '"Masaki Hashiyada"' showing total 8 results
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8 results on '"Masaki Hashiyada"'

1. Characterization of cytoskeletal and structural effects of INF2 variants causing glomerulopathy and neuropathy

Author

Hiroko Ueda, Quynh Thuy Huong Tran, Linh Nguyen Truc Tran, Koichiro Higasa, Yoshiki Ikeda, Naoyuki Kondo, Masaki Hashiyada, Chika Sato, Yoshinori Sato, Akira Ashida, Saori Nishio, Yasunori Iwata, Hiroyuki Iida, Daisuke Matsuoka, Yoshihiko Hidaka, Kenji Fukui, Suzu Itami, Norihito Kawashita, Keisuke Sugimoto, Kandai Nozu, Motoshi Hattori, and Hiroyasu Tsukaguchi

Subjects
Medicine, Science
Abstract

Abstract Focal segmental glomerulosclerosis (FSGS) is a common glomerular injury leading to end-stage renal disease. Monogenic FSGS is primarily ascribed to decreased podocyte integrity. Variants between residues 184 and 245 of INF2, an actin assembly factor, produce the monogenic FSGS phenotype. Meanwhile, variants between residues 57 and 184 cause a dual-faceted disease involving peripheral neurons and podocytes (Charcot–Marie–Tooth CMT/FSGS). To understand the molecular basis for INF2 disorders, we compared structural and cytoskeletal effects of INF2 variants classified into two subgroups: One (G73D, V108D) causes the CMT/FSGS phenotype, and the other (T161N, N202S) produces monogenic FSGS. Molecular dynamics analysis revealed that all INF2 variants show distinct flexibility compared to the wild-type INF2 and could affect stability of an intramolecular interaction between their N- and C-terminal segments. Immunocytochemistry of cells expressing INF2 variants showed fewer actin stress fibers, and disorganization of cytoplasmic microtubule arrays. Notably, CMT/FSGS variants caused more prominent changes in mitochondrial distribution and fragmentation than FSGS variants and these changes correlated with the severity of cytoskeletal disruption. Our results indicate that CMT/FSGS variants are associated with more severe global cellular defects caused by disrupted cytoskeleton-organelle interactions than are FSGS variants. Further study is needed to clarify tissue-specific pathways and/or cellular functions implicated in FSGS and CMT phenotypes

Published
2023
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2. Association of Neonatal Jaundice with Gut Dysbiosis Characterized by Decreased Bifidobacteriales

Author

Shohei Akagawa, Yuko Akagawa, Sohsaku Yamanouchi, Yoshiki Teramoto, Masahiro Yasuda, Sadayuki Fujishiro, Jiro Kino, Masato Hirabayashi, Kenji Mine, Takahisa Kimata, Masaki Hashiyada, Atsushi Akane, Shoji Tsuji, and Kazunari Kaneko

Subjects
microbiota, neonatal jaundice, bilirubin, 16S rRNA gene sequencing, Bifidobacteriales, Microbiology, QR1-502
Abstract

Neonatal jaundice, caused by excess serum bilirubin levels, is a common condition in neonates. Imbalance in the gut microbiota is believed to play a role in the development of neonatal jaundice. Thus, we aimed to reveal the gut microbiota characteristics in neonates with jaundice. 16S rRNA gene sequencing was performed on stool samples collected on day 4 from 26 neonates with jaundice (serum total bilirubin > 15.0 mg/dL) and 17 neonates without jaundice (total serum bilirubin < 10.0 mg/dL). All neonates were born full term, with normal weight, by vaginal delivery, and were breastfed. Neonates who were administered antibiotics, had serum direct bilirubin levels above 1 mg/dL, or had conditions possibly leading to hemolytic anemia were excluded. The median serum bilirubin was 16.0 mg/dL (interquartile range: 15.5–16.8) and 7.4 mg/dL (interquartile range: 6.8–8.3) for the jaundice and non-jaundice groups, respectively. There was no difference in the alpha diversity indices. Meanwhile, in the jaundice group, linear discriminant analysis effect size revealed that Bifidobacteriales were decreased at the order level, while Enterococcaceae were increased and Bifidobacteriaceae were decreased at the family level. Bifidobacteriaceae may act preventatively because of their suppressive effect on beta-glucuronidase, leading to accelerated deconjugation of conjugated bilirubin in the intestine. In summary, neonates with jaundice had dysbiosis characterized by a decreased abundance of Bifidobacteriales.

Published
2021
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3. Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study

Author

Yoko Nakai, Shohei Akagawa, Sadayuki Fujishiro, Yuko Akagawa, Mitsuru Yamagishi, Sohsaku Yamanouchi, Takahisa Kimata, Atsushi Ohashi, Masaki Hashiyada, Atsushi Akane, Shoji Tsuji, and Kazunari Kaneko

Subjects
Nutrition and Dietetics, Medicine (miscellaneous)
Abstract

Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota. However, studies on the composition of gut microbiota in children with SMIDs are limited. Therefore, we aimed to examine the characteristics of the gut microbiota in children with SMIDs.16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years). Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups. Daily dietary fiber intake in the SMID group was evaluated using questionnaires.The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group. Beta diversity analysis identified different clusters. Compared with the HC group, Clostridiales and butyric acid-producing bacteria were less abundant and Bacteroidales were more abundant in the SMID group. Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake. Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.

Published
2022
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5. Dysbiosis in Gut Microbiota in Children Born Preterm Who Developed Autism Spectrum Disorder: A Pilot Study

Author

Sadayuki Fujishiro, Shoji Tsuji, Shohei Akagawa, Yuko Akagawa, Sohsaku Yamanouchi, Yuko Ishizaki, Masaki Hashiyada, Atsushi Akane, and Kazunari Kaneko

Subjects
Developmental and Educational Psychology
Abstract

The gut microbiota was reported to differ between children with autism spectrum disorder (ASD) and typically developing (TD) children, and dysbiosis of the gut microbiota in preterm infants is common. Here, we explored the characteristics of gut microbiota in children born preterm with ASD. We performed 16S rRNA gene sequencing using stool samples from ASD children born preterm and TD children born preterm. Alpha diversity was significantly greater in the ASD group. A comparison of beta diversity showed different clusters. Linear discriminant analysis effect size analysis revealed significantly more Firmicutes in the ASD group compared with the TD group. In conclusion, the gut microbiota in children born preterm differs between children with ASD and TD.

Published
2022

6. Allele frequencies of 31 autosomal short tandem repeat (auSTR) loci obtained using the Precision ID GlobalFiler™ NGS STR Panel v2 in 322 individuals from the Japanese population

Author

Tsukasa Ohuchi, Xueting Guan, Eriko Hirai, Masaki Hashiyada, Sho Manabe, Atsushi Akane, Noboru Adachi, Keiji Tamaki, and Masato Funayama

Subjects
Issues, ethics and legal aspects, Japan, Gene Frequency, Humans, Sequence Analysis, DNA, DNA Fingerprinting, Pathology and Forensic Medicine, Microsatellite Repeats
Abstract

In human identification methods that target short tandem repeats (STRs), massively parallel sequencing (MPS) technology has made it possible to genotype at the level of the specific sequence itself. This allows for the detection of repeat unit variants and single nucleotide polymorphisms (SNPs) adjacent to the STRs. Using the GlobalFiler™ NGS STR Panel v2, Ion S5, and Converge software, this study constructed a Japanese database of 31 autosomal STRs (auSTRs) and two sex markers from 322 individuals. After excluding some sequence errors and stutters, a total of 31 novel alleles were identified. Additionally, using the allele frequencies of 31 auSTR loci, the match probabilities for the length-based and sequence-based data were calculated to be 1.433 × 10

Published
2022

8. Corrigendum to 'Evaluation of probability distribution models for stutter ratios in the typing system of GlobalFiler and 3500xL Genetic Analyzer' [Leg. Med. 52 (2021) 101906]

Author

Atsushi Akane, Takashi Fukagawa, Koji Fujii, Kazumasa Sekiguchi, Keiji Tamaki, Masaki Hashiyada, Sho Manabe, Kana Inoue, and Natsuko Mizuno

Subjects
Issues, ethics and legal aspects, Spectrum analyzer, Computer science, business.industry, Probability distribution, Pattern recognition, Artificial intelligence, Typing, business, Pathology and Forensic Medicine
Published
2022
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