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IntroductionJapan’s teikei movement, recognized as a source of inspiration for Community Supported Agriculture (CSA) in Western countries, is now entering its fifth decade. Built upon trust and shared values, teikei has continued to rely on mutually supportive relationships between organic producers and consumers. The movement’s commitments were originally articulated through the ten principles of teikei, which offer a roadmap to create food systems based on solidarity principles going beyond market transactions. Despite a decline in numbers, teikei groups continue to operate in the midst of societal shifts that are altering food practices and consumption patterns. These changes have had an impact on the implementation of the ten principles and on the power dynamics between producers and consumers.MethodsThis research investigates how such shifts have affected the development of alternative food systems in Japan, the evolution of teikei as a social movement, and the tensions that arise from contrasting notions of agri-food system alterity rooted in decommodified relationships versus market-based transactions. We employ the ten principles as a framework to investigate the transformations of some representative teikei groups over time, and identify three types of shifts: relational, operational, and ideological. These shifts show how different teikei actors have been engaging in realizing the vision of building sustainable agri-food systems through alternative market relations.ResultsThe shifts also underscore the fluid and situated nature of agri-food system alterity within historical, geographical, and cultural relational spaces. The current variations of teikei configurations and the progressive diversification of approaches to address the challenges of upholding the original principles demonstrate the movement’s adaptability over time. However, they also demonstrate the necessity to strike a compromise between conflicting needs.DiscussionThe development of the teikei movement is not only important from an historical and geographically-situated perspective, but also as a dynamic and evolving experiment in the potential and challenges of active food citizenship. The democratic decision-making processes embedded within teikei principles and practices offer a valuable model for understanding how individuals enact their food citizenship and contribute to ongoing transformation of the agri-food system. Simultaneously, these shifts also serve as a warning against how democratic principles can be eroded by conventionalization and neoliberalization, and about the assumptions that arise during the process of building alternative agri-food systems, such as gendered labor.

Imagining sustainable food futures is key to effectively transforming food systems. Yet even transdisciplinary approaches struggle to open up complex and highly segregated food policy governance for co-production and can fail to critically interrogate assumptions, worldviews, and values. In this Perspective we argue that transdisciplinary processes concerned with sustainable food system transformation need to meaningufully engage with critical food futures, and can do so through the use of soft scenario methods to learn about, play with, and experiment in futures. Specifically, soft scenarios contribute in four ways: 1) questioning widely held assumptions about the future; 2) being inclusive to multiple perspectives and worldviews; 3) fostering receptiveness to unimaginable futures; 4) developing futures literacy. Based on insights from a 5-year transdisciplinary action research project on sustainable food transformation across Asia, we demonstrate how these processes play out in narratives, serious games and interactive art featuring soft scenarios. We conclude by discussing the potential for collaboration between transdisciplinary and futures researchers, especially for transforming food systems.

We previously reported that Lys175 in the region of the active site of chymotrypsin (Csin) could be site-selectively modified by using an N-hydroxy succinimide (NHS) ester of the peptidyl derivative containing 1-amino-2-ethylphenylphosphonate diphenyl ester [NHS-Suc-Ala-Ala-PheP(OPh)2]. In this study, the Lys175-selective modification method was expanded to incorporate functional groups into Lys 175 in Csin. Two types of peptidyl phosphonate derivatives with the dansyl group (Dan) as a functional molecule, Dan-β-Ala-[Asp(NHS) or Glu(NHS)]-Ala-Ala-(R)-PheP(OPh)2 (DanD and DanE, respectively), were synthesized, and their action was evaluated when modifying Lys175 in Csin. Ion-exchange chromatography (IEC), fluorescence spectroscopy, and LC-MS/MS were used to analyze the products from the reaction of Csin with DanD or DanE. By IEC and LC-MS/MS, the results showed that DanE reacted with Csin more effectively than DanD to produce the modified Csin (DanMCsin) bearing Dan at Lys175. DanMCsin exhibited an enzymatic activity corresponding to 1/120 of Csin against Suc-Ala-Ala-Phe-pNA. In addition, an effect of Lys175 modification on the access of the proteinaceous Bowman–Birk inhibitor to the active site of DanMCsin was investigated. In conclusion, by using a peptidyl derivative containing 1-amino-2-ethylphenylphosphonate diphenyl ester, we demonstrated that a functional group could be incorporated into Lys175 in Csin.

Games offer unique possibilities for imagining and experimenting with new systems of governance for more sustainable futures – new rules and institutions, new roles, and new dynamic worlds. However, research on sustainability games has mostly investigated games as a type of futures method, largely divorced from its societal contexts. In this paper, we argue that to unlock the potential of gaming for anticipatory governance in the service of a more sustainable future, it is important take a whole-society perspective, and examine the possibilities and challenges offered by contextual factors. Using the Netherlands and Japan as examples, we investigate the following questions: 1) How do governance cultures allow or restrict opportunities for the participatory exploration of futures using games? 2) How does, and can, the game sector in a given context support anticipatory gaming? 3) How do dominant societal relationships with games limit, and offer opportunities for, gaming for anticipatory governance?

(1) Background: When measuring anaerobic work threshold (AT), the conventional V-slope method includes the subjectivity of the examiner, which cannot be eliminated completely. Therefore, we implemented an engineering method using strucchange to objectively search for the inflection point of AT. (2) Methods: Seventeen subjects (15 men and 2 women) were included in the study. The subjects rode an ergometer and performed a ramp load test for 18 min and 30 s. (3) Results: In VE (Ventilation), 11 out of 12 subjects had the same results with 95% confidence intervals for the AT by the strucchange and respiratory metabolic apparatus. In VCO2 (Carbon dioxide emissions), 9 out of 12 subjects had the same results with 95% confidence intervals for the AT with the strucchange and respiratory metabolic apparatus. In VE, 3 out of 12 subjects showed the same results for respiratory metabolic analysis and the AT by the V-slope method. In VCO2, 3 out of 12 subjects showed the same results for the respiratory metabolic analysis and AT by the V-slope method in VCO2. (4) Conclusions: Strucchange was more objective and significant in identifying the AT than the V-slope method.