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BackgroundPost-acute COVID-19 syndrome patients complain of sensory alterations, mainly positive symptoms such as paresthesia or neuropathic pain but also decreased tactile sensation. Using the Semmes–Weinstein monofilament test (SWMT), our study aims to confront recently infected SARS-CoV2 subjects with a control group.MethodsThis is a cross-sectional, single-centric study. We performed the SWMT (North Coast Medical Inc.) on 30 patients with previous SARS-CoV2 infection (COVID group) and 46 controls (control group). These patients did not present comorbidities or sensory impairment and did not take any medications. The control group tested negative for SARS-CoV2 infection since the COVID-19 pandemic; the COVID group was examined for this study after the resolution of the infection. We tested the threshold of tactile sensation of the tips of the thumb, index, and little finger of each hand, one hand at a time; the dorsum and the hypothenar regions were also tested.ResultsBoth groups presented the perception of tactile sensation within the reference value. Despite this result, subclinical changes suggestive of the involvement in peripheral sensory nerve function have been identified in the tested sites in the COVID group compared to the control group. The overall mean target force (grams) was higher in the COVID group than in the control group: 27 (7) vs. 19 (10) mg, p < 0.001.ConclusionControls and the COVID group infection had normal tactile sensation thresholds. However, the COVID group presented a higher threshold than the control group, suggesting a possible subclinical perception of tactile sensation involvement of A-beta nerve fibers.

Malignant pleural mesothelioma (MPM) is an aggressive tumour resistant to treatments. It has been postulated that cancer stem cells (CSCs) persist in tumours causing relapse after multimodality treatment. In the present study, a novel miRNA-based therapy approach is proposed. MPM-derived spheroids have been treated with exosome-delivered miR-126 (exo-miR) and evaluated for their anticancer effect. The exo-miR treatment increased MPM stem-cell like stemness and inhibited cell proliferation. However, at a prolonged time, the up taken miR-126 was released by the cells themselves through exosomes; the inhibition of exosome release by an exosome release inhibitor GW4869 induced miR-126 intracellular accumulation leading to massive cell death and in vivo tumour growth arrest. Autophagy is involved in these processes; miR-126 accumulation induced a protective autophagy and the inhibition of this process by GW4869 generates a metabolic crisis that promotes necroptosis, which was associated with PARP-1 over-expression and cyt-c and AIF release. Here, for the first time, we proposed a therapy against CSCs, a heterogeneous cell population involved in cancer development and relapse.

The cold response of the digital arteries in a cohort of vibration-exposed workers was related to measures of daily vibration exposure expressed in terms of r.m.s. acceleration magnitude normalised to an 8-hour day, and frequency was weighted according to either the frequency weighting Wh defined in ISO 5349-1:2001 (Ah(8) in ms−2 r.m.s) or the hand–arm vascular frequency weighting Wp proposed in the ISO Technical Report 18570:2017 (Ap(8) in ms−2 r.m.s.). The metric Ap(8), which assigns more weight to intermediate- and high-frequency vibrations (31.5–250 Hz), performed better for the prediction of cold-induced digital arterial hyperresponsiveness in the vibration-exposed workers than the measure Ah(8) derived from the conventional ISO frequency weighting, which gives more importance to lower-frequency vibrations (≤16 Hz).

Workers can be exposed daily to foot-transmitted vibration (FTV) from standing on mobile equipment or vibrating platforms and surfaces. This results in a consistent risk of developing neurological, vascular, and musculoskeletal problems. To date, there are no international standards describing procedures with which to evaluate the health risks deriving from long-term exposure to FTV. To study the applicability of hand–arm vibration (HAV) standards to the foot, the biomechanical responses of the hand and foot in terms of the frequency response function upon varying contact conditions were compared. Results evidenced similarities between the responses of the wrist and ankle, with differences in resonance for the fingers and toes. The study confirms that HAV standards are more suitable than whole-body vibration standards for evaluating higher frequency exposure to FTV.