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ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. The involvement of ADP-ribosylation in the life cycle of Dictyostelium and some filamentous fungi has also been demonstrated. However, the role of this process in pathogenic oomycetes has never been addressed. Here, we show that the Phytophthora infestans genome contains two PARP-like protein genes (PiPARP1 and PiPARP2), and provide evidence of PARylation activity for one of them (PiPARP2). Using dsRNA-mediated RNA silencing of the PiPARP2 gene and chemical (pharmacological) inhibition of PARP activity by 3-aminobenzamide (3AB) PARP inhibitor, we demonstrate the critical functional role of ADP-ribosylation in Phytophthora mycelium growth. Virulence test on detached leaves also suggests an important role of ADP-ribosylation in Phytophthora host plant colonisation and pathogenesis. On a practical level, our data suggest that targeting the PARylation system may constitute a novel powerful approach for the management of Phytophthora diseases. [ABSTRACT FROM AUTHOR]

ADP-ribosylation (ADPRylation) is a mechanism which post-translationally modifies proteins in eukaryotes in order to regulate a broad range of biological processes including programmed cell death, cell signaling, DNA repair, and responses to biotic and abiotic stresses. Poly(ADP-ribosyl) polymerases (PARPs) play a key role in the process of ADPRylation, which modifies target proteins by attaching ADP-ribose molecules. Here, we investigated whether and how PARP1 and PARylation modulate responses of Nicotiana benthamiana plants to methyl viologen (MV)-induced oxidative stress. It was found that the burst of reactive oxygen species (ROS), cell death, and loss of tissue viability invoked by MV in N. benthamiana leaves was significantly delayed by both the RNA silencing of the PARP1 gene and by applying the pharmacological inhibitor 3-aminobenzamide (3AB) to inhibit PARylation activity. This in turn reduced the accumulation of PARylated proteins and significantly increased the gene expression of major ROS scavenging enzymes including SOD (NbMnSOD; mitochondrial manganese SOD), CAT (NbCAT2), GR (NbGR), and APX (NbAPX5), and inhibited cell death. This mechanism may be part of a broader network that regulates plant sensitivity to oxidative stress through various genetically programmed pathways. [ABSTRACT FROM AUTHOR]

The Pleistocene-Holocene climate transition resulted in a dramatic reduction in ground-water recharge in many aquifers in arid and semiarid regions throughout the world. This study conducted numerical experiments to compare the evolution of groundwater hydraulics and age patterns in arid and semiarid aquifers in response to transient conditions associated with recharge decline from the Pleistocene to the Holocene. Our results show that after a rapid reduction in recharge, the amplitude of water-table undulations and regional ground-water slope both reduced. This resulted in a general, and relatively rapid, contraction of local flow systems and an increase in the extent of intermediate and regional systems. The previous hierarchy of local, intermediate, and regional flow systems was completely replaced by largely horizontal and regional flow patterns after -10,000 yr. However, in stark contrast, we observed that the original Pleistocene age patterns have remained almost unchanged throughout the 10,000 yr Holocene period. Thus, groundwater age is more likely to be indicative of past rather than current flow systems. Consequently, due to this age persistence, the use of modern groundwater age data to calibrate models or compute recharge with methods that do not account for this potentially significant spatial and temporal mismatch between age and hydraulics will be misleading and erroneous. This has significant implications for hydrogeologic analyses. The findings of this study may also apply to areas that have undergone dramatic changes in land cover or land use that strongly influence transient groundwater recharge processes. [ABSTRACT FROM AUTHOR]