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Abstract Background With the improvement of living standards, implant dentures have become the choice of more and more patients with missing teeth. Dentures are not natural teeth, and may cause different degrees of negative impacts on patients in physiological, psychological and social aspects. The objective of this study was to examine the current status of psychological resilience among implant denture patients and the factors that influence it. Additionally, we aimed to analyse the correlation between psychological resilience, social support, and oral health behaviours, with a view to providing a scientific basis for the development of targeted interventions for patients with implant dentures. Methods A survey of 238 patients with implant dentures was conducted in the Department of Stomatology of the Affiliated Hospital of Jiangnan University and Wuxi Stomatological Hospital. The survey included a self-designed general information questionnaire, disease condition questionnaire, The Connor-Davidson Resilience Scale (CD-RISC), The Social Support Rating Scale (SSRS), and The oral health behavior scale. Results The CD-RISC score of patients with implant dentures was 66.49 ± 15.59, while the SSRS score was 37.90 ± 7.73, and the oral health behaviour score was 34.73 ± 7.92. Multiple linear regression analysis showed that age, economic status, social support, and oral health behavioural status were factors influencing the psychological resilience of patients with implant dentures (all P

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease. Four major genes associated with ALS—SOD1, TARDBP, FUS, and C9orf72—have been identified, with the fused in sarcoma (FUS) gene demonstrating considerable genetic heterogeneity. Our research group has previously established an induced pluripotent stem (iPS) cell line harboring the c.1562G > A mutation in the FUS gene. The objective of this study is to create another iPS cell line featuring the pathogenic c.1126C > T mutation in the FUS gene. This research aims not only to establish a disease model for ALS linked to FUS mutations but also to pave the way for potential therapeutic interventions.

With global warming accelerating, the heavily populated and rapidly urbanized coastal regions of the Pearl River Delta (PRD) and the Yangtze River Delta (YRD) stand as representative areas with mounting concerns about extreme heat stress. This study analyzes differentiated effects of temperature (TAS) and relative humidity (RH) on human heat stress measured by wet-bulb globe temperature (WBGT) in those urban regions based on machine learning and mathematical derivation, while also examining the impacts of global warming and urbanization on prospective heat risks. To generate fine-scale climate projections targeted at the PRD and YRD, two global projections forced by Representative Concentration Pathway (RCP) 8.5 scenario are dynamically downscaled using non-hydrostatic Regional Climate Model version 4.7 (RegCM4), with the urban density and extent updated every year based on Shared Socioeconomic Pathways 5-8.5 (SSP5) scenario, thereby incorporating the transient urban growth into future projections. The bias-corrected downscaled simulations effectively capture the distinct interdependencies between TAS and RH on WBGT across different regions, similar to the observed patterns during the historical period. While the absolute contribution of TAS to WBGT is larger than RH regardless of warming levels and regions, the relative increase in RH becomes more pronounced with warming. Under RCP8.5 scenario, unprecedentedly extreme WBGT is projected to emerge in the far future (2080–2099). In contrast, the effect of urbanization appears to be more dominant in the near future (2030–2049) as urban density under SSP5 scenario is projected to peak around the 2040s and gradually decrease afterwards. The reduction of RH is found in the intensely urbanized areas locally, but it does not significantly lower WBGT because the positive contribution of increased TAS is more dominant. As a result, highly urbanized regions still exhibit higher WBGT compared to other areas. In addition, urban heat island effect is more pronounced for compact areas with high urban density (i.e., PRD) and at night. Despite the smaller temperature increase from urban heat island effect compared to global warming, it can play a critical role in exacerbating heat stress, adding to the already dangerous humid and hot conditions.

The Simplified Wet Bulb Globe Temperature (sWBGT) is widely used in heat stress assessments for climate-change studies, but its limitations have not been thoroughly explored. Building on recent critiques of sWBGT's use for current climate on global scale, this study examines sWBGT's biases using dynamically-downscaled sub-daily climate projections under multiple future emission scenarios. The analysis is aimed at understanding caveats in the application of sWBGT and the uncertainties in existing climate change analysis dependent on sWBGT. Results indicate sWBGT's biases are heavily influenced by local near-surface air temperature, with overestimation of heat stress in East Asia regions, particularly hot and humid areas, due to static assumptions of radiation and wind speed. This overestimation is amplified in warmer climates, leading to exaggerated projected heat stress increases in future. In contrast, underestimations are found for heat stress levels attributed to low wind speeds and strong radiations, such as over the Tibetan Plateau and certain extreme events. Additionally, sWBGT underestimates variability in extreme heatwave events compared to WBGT in both current and future climates, irrespective of overestimation in absolute heatwave intensities. This study emphasizes the limitations of sWBGT, especially in future warmer climates. Importance of sub-daily data for capturing daily maximum heat stress level and reflecting diurnal variations in different components is also discussed. In conclusion, we recommend using Liljegren's model (i.e., physics-based calculation) with high-resolution sub-daily climate data for more accurate outdoor heat stress assessments in climate change studies.

Vaccarin is a flavonoid glycoside, which has a variety of pharmacological properties and plays a protective role in diabetes and its complications, but its mechanism is unclear. In this study, we aim to investigate whether histone deacetylase 1(HDAC1), a gene that plays a pivotal role in regulating eukaryotic gene expression, is the target of miR-570-3p in diabetic vascular endothelium, and the potential molecular mechanism of vaccarin regulating endothelial inflammatory injury through miR-570-3p/HDAC1 pathway. The HFD and streptozotocin (STZ) induced diabetes mice model, a classical type 2 diabetic model, was established. The aorta of diabetic mice displayed a decrease of miR-570-3p, the elevation of HDAC1, and inflammatory injury, which were alleviated by vaccarin. Next, we employed the role of vaccarin in regulating endothelial cells miR-570-3p and HDAC1 under hyperglycemia conditions in vitro. We discovered that overexpression of HDAC1 counteracted the inhibitory effect of vaccarin on inflammatory injury in human umbilical vein endothelial cells (HUVECs). Manipulation of miRNA levels in HUVECs was achieved by transfecting cells with miR-570-3p mimic and inhibitor. Overexpression of miR-570-3p could decrease the expression of downstream components of HDAC1 including TNF-α, IL-1β, and malondialdehyde, while increasing GSH-Px activity in HUVECs under hyperglycemic conditions. Nevertheless, such phenomenon was completely reversed by miR-570-3p inhibitor, and administration of miR-570-3p inhibitor could block the inhibition of vaccarin on HDAC1 and inflammatory injury. Luciferase reporter assay confirmed the 3′- UTR of the HDAC1 gene was a direct target of miR-570-3p. In summary, our findings suggest that vaccarin alleviates endothelial inflammatory injury in diabetes by mediating miR-570-3p/HDAC1 pathway. Our study provides a new pathogenic link between deregulation of miRNA expression in the vascular endothelium of diabetes and inflammatory injury and provides new ideas, insights, and choices for the scope of application and medicinal value of vaccarin and some potential biomarkers or targets in diabetic endothelial dysfunction and vascular complications.

[Objectives] To investigate the mechanisms and pharmacologic effects of Citri Reticulatae Pericarpium against keloids by network pharmacology systematically. [Methods] TCMSP, Uniprot and BATMAN-TCM databases were used to obtain the active constituents and targets of Citri Reticulatae Pericarpium. "Keloid" was used as key word to search for related therapeutic targets from Drug Bank, OMIM, TTD, and GEO databases. The Chinese medicine compound-target network was constructed by Cytoscape software. Besides, gene ontology (GO) and Kyoto Encyclopedia of genes and genome enrichment analysis were also performed. Afterward, Discovery Studio software was used to assess the interaction of key components and genes. [Results] Five active components of Citri Reticulatae Pericarpium, 773 compound targets and 676 keloid treatment targets were obtained in the databases. After the intersection, there are 47 targets of Citri Reticulatae Pericarpium for treating keloids. Hub genes were identified such as MMP9, IL6, TNF, TP53, and VEGFA, which were enriched in tumor necrosis factor-α, nuclear factor kappa-B, and other signaling pathways. The molecular docking stimulation confirmed the interaction between the MMP9 and three components of Citri Reticulatae Pericarpium. [Conclusions] Citri Reticulatae Pericarpium may play an important role in treating keloids through modulating genes and signaling pathways. The present study sheds light on the mechanisms of active compounds of Citri Reticulatae Pericarpium for the treatment of keloids. [ABSTRACT FROM AUTHOR]

This paper reviewed the shortcomings of conventional therapies for ischemic stroke and discussed the methods of vascular regenera- tion, stem cell differentiation, homing and neuronal remodeling after ischemic stroke, to provide potential therapeutic ideas for ischemic stroke. [ABSTRACT FROM AUTHOR]